Procaterol-stimulated Increases in Ciliary Bend Amplitude and Ciliary Beat Frequency in Mouse Bronchioles
The beating cilia play a key role in lung mucociliary transport. The ciliary beating frequency (CBF) and ciliary bend amplitude (CBA) of isolated mouse bronchiolar ciliary cells were measured using a light microscope equipped with a high-speed camera (500 Hz). Procaterol (aβ 2 -agonist) increased CB...
Gespeichert in:
| Veröffentlicht in: | Cellular physiology and biochemistry 2012-01, Vol.29 (3-4), p.511-522 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 522 |
|---|---|
| container_issue | 3-4 |
| container_start_page | 511 |
| container_title | Cellular physiology and biochemistry |
| container_volume | 29 |
| creator | Komatani-Tamiya, Nobuyo Daikoku, Eriko Takemura, Yoshizumi Shimamoto, Chikao Nakano, Takashi Iwasaki, Yoshinobu Kohda, Yuka Matsumura, Hitoshi Marunaka, Yoshinori Nakahari, Takashi |
| description | The beating cilia play a key role in lung mucociliary transport. The ciliary beating frequency (CBF) and ciliary bend amplitude (CBA) of isolated mouse bronchiolar ciliary cells were measured using a light microscope equipped with a high-speed camera (500 Hz). Procaterol (aβ 2 -agonist) increased CBA and CBF in a dose dependent manner via cAMP. The time course of CBA increase is distinct from that of CBF increase: procaterol at 10 nM first increased CBA and then CBF. Moreover, 10 pM procaterol increased CBA, not CBF, whereas 10 nM procaterol increased both CBA and CBF. Concentration-response studies of procaterol demonstrated that the CBA curve was shifted to a lower concentration than the CBF curve, which suggests that CBA regulation is different from CBF regulation. Measurements of microbead movements on the bronchiole of lung slices revealed that 10 pM procaterol increased the rate of ciliary transport by 37% and 10 nM procaterol increased it by 70%. In conclusion, we have shown that increased CBA is of particular importance for increasing the bronchiolar ciliary transport rate, although CBF also plays a role in increasing it. |
| doi_str_mv | 10.1159/000338505 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_22508058</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1002511792</sourcerecordid><originalsourceid>FETCH-LOGICAL-c435t-bddb41f304bb228064a8c196ce1cefbc201ff4bea602b97bf1e097f44cd8a0a53</originalsourceid><addsrcrecordid>eNptkDtPwzAUhS0EouUxsCMUiQWGwLUTN87YVhQqFdEB5sh2rsGQR7GTof8eVy1lYbKP7udzjw8hFxTuKOX5PQAkieDAD8iQpozGeZaJw3AHymORi2xATrz_hCCznB2TAWMcBHAxJHbpWi07dG0V-87WfRVEGc0b7VB69JFtoqmtrHTraIJNGY3rVWW7vsRIBvU3kl00c_jdY6PXm0fPbe8xmri20R-2rdCfkSMjK4_nu_OUvM0eXqdP8eLlcT4dL2KdJryLVVmqlJoEUqUYEzBKpdA0H2mkGo3SDKgxqUI5AqbyTBmKkGcmTXUpJEienJKbre_KtSGO74raeo1VJRsMmQoKwDiloYiA3m5R7VrvHZpi5Wwd_hOgYtNssW82sFc7217VWO7J3yoDcL0FvqR7R7cHpsvJ1qJYlSZQl_9Suy0_au6JSw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1002511792</pqid></control><display><type>article</type><title>Procaterol-stimulated Increases in Ciliary Bend Amplitude and Ciliary Beat Frequency in Mouse Bronchioles</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Komatani-Tamiya, Nobuyo ; Daikoku, Eriko ; Takemura, Yoshizumi ; Shimamoto, Chikao ; Nakano, Takashi ; Iwasaki, Yoshinobu ; Kohda, Yuka ; Matsumura, Hitoshi ; Marunaka, Yoshinori ; Nakahari, Takashi</creator><creatorcontrib>Komatani-Tamiya, Nobuyo ; Daikoku, Eriko ; Takemura, Yoshizumi ; Shimamoto, Chikao ; Nakano, Takashi ; Iwasaki, Yoshinobu ; Kohda, Yuka ; Matsumura, Hitoshi ; Marunaka, Yoshinori ; Nakahari, Takashi</creatorcontrib><description>The beating cilia play a key role in lung mucociliary transport. The ciliary beating frequency (CBF) and ciliary bend amplitude (CBA) of isolated mouse bronchiolar ciliary cells were measured using a light microscope equipped with a high-speed camera (500 Hz). Procaterol (aβ 2 -agonist) increased CBA and CBF in a dose dependent manner via cAMP. The time course of CBA increase is distinct from that of CBF increase: procaterol at 10 nM first increased CBA and then CBF. Moreover, 10 pM procaterol increased CBA, not CBF, whereas 10 nM procaterol increased both CBA and CBF. Concentration-response studies of procaterol demonstrated that the CBA curve was shifted to a lower concentration than the CBF curve, which suggests that CBA regulation is different from CBF regulation. Measurements of microbead movements on the bronchiole of lung slices revealed that 10 pM procaterol increased the rate of ciliary transport by 37% and 10 nM procaterol increased it by 70%. In conclusion, we have shown that increased CBA is of particular importance for increasing the bronchiolar ciliary transport rate, although CBF also plays a role in increasing it.</description><identifier>ISSN: 1015-8987</identifier><identifier>EISSN: 1421-9778</identifier><identifier>DOI: 10.1159/000338505</identifier><identifier>PMID: 22508058</identifier><language>eng</language><publisher>Basel, Switzerland</publisher><subject>Adrenergic beta-2 Receptor Agonists - pharmacology ; Albuterol - pharmacology ; Animals ; Axoneme - metabolism ; Axoneme - physiology ; Bronchioles - drug effects ; Bronchioles - metabolism ; Bronchioles - physiology ; Calcium - pharmacology ; Cilia - drug effects ; Cilia - metabolism ; Cilia - physiology ; Cyclic AMP - metabolism ; Dose-Response Relationship, Drug ; Epithelium - drug effects ; Epithelium - metabolism ; Epithelium - physiology ; Female ; In Vitro Techniques ; Mice ; Mice, Inbred C57BL ; Microspheres ; Mucociliary Clearance ; Original ; Procaterol - pharmacology ; Time Factors</subject><ispartof>Cellular physiology and biochemistry, 2012-01, Vol.29 (3-4), p.511-522</ispartof><rights>2012 S. Karger AG, Basel</rights><rights>Copyright © 2012 S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-bddb41f304bb228064a8c196ce1cefbc201ff4bea602b97bf1e097f44cd8a0a53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22508058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Komatani-Tamiya, Nobuyo</creatorcontrib><creatorcontrib>Daikoku, Eriko</creatorcontrib><creatorcontrib>Takemura, Yoshizumi</creatorcontrib><creatorcontrib>Shimamoto, Chikao</creatorcontrib><creatorcontrib>Nakano, Takashi</creatorcontrib><creatorcontrib>Iwasaki, Yoshinobu</creatorcontrib><creatorcontrib>Kohda, Yuka</creatorcontrib><creatorcontrib>Matsumura, Hitoshi</creatorcontrib><creatorcontrib>Marunaka, Yoshinori</creatorcontrib><creatorcontrib>Nakahari, Takashi</creatorcontrib><title>Procaterol-stimulated Increases in Ciliary Bend Amplitude and Ciliary Beat Frequency in Mouse Bronchioles</title><title>Cellular physiology and biochemistry</title><addtitle>Cell Physiol Biochem</addtitle><description>The beating cilia play a key role in lung mucociliary transport. The ciliary beating frequency (CBF) and ciliary bend amplitude (CBA) of isolated mouse bronchiolar ciliary cells were measured using a light microscope equipped with a high-speed camera (500 Hz). Procaterol (aβ 2 -agonist) increased CBA and CBF in a dose dependent manner via cAMP. The time course of CBA increase is distinct from that of CBF increase: procaterol at 10 nM first increased CBA and then CBF. Moreover, 10 pM procaterol increased CBA, not CBF, whereas 10 nM procaterol increased both CBA and CBF. Concentration-response studies of procaterol demonstrated that the CBA curve was shifted to a lower concentration than the CBF curve, which suggests that CBA regulation is different from CBF regulation. Measurements of microbead movements on the bronchiole of lung slices revealed that 10 pM procaterol increased the rate of ciliary transport by 37% and 10 nM procaterol increased it by 70%. In conclusion, we have shown that increased CBA is of particular importance for increasing the bronchiolar ciliary transport rate, although CBF also plays a role in increasing it.</description><subject>Adrenergic beta-2 Receptor Agonists - pharmacology</subject><subject>Albuterol - pharmacology</subject><subject>Animals</subject><subject>Axoneme - metabolism</subject><subject>Axoneme - physiology</subject><subject>Bronchioles - drug effects</subject><subject>Bronchioles - metabolism</subject><subject>Bronchioles - physiology</subject><subject>Calcium - pharmacology</subject><subject>Cilia - drug effects</subject><subject>Cilia - metabolism</subject><subject>Cilia - physiology</subject><subject>Cyclic AMP - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - metabolism</subject><subject>Epithelium - physiology</subject><subject>Female</subject><subject>In Vitro Techniques</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microspheres</subject><subject>Mucociliary Clearance</subject><subject>Original</subject><subject>Procaterol - pharmacology</subject><subject>Time Factors</subject><issn>1015-8987</issn><issn>1421-9778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkDtPwzAUhS0EouUxsCMUiQWGwLUTN87YVhQqFdEB5sh2rsGQR7GTof8eVy1lYbKP7udzjw8hFxTuKOX5PQAkieDAD8iQpozGeZaJw3AHymORi2xATrz_hCCznB2TAWMcBHAxJHbpWi07dG0V-87WfRVEGc0b7VB69JFtoqmtrHTraIJNGY3rVWW7vsRIBvU3kl00c_jdY6PXm0fPbe8xmri20R-2rdCfkSMjK4_nu_OUvM0eXqdP8eLlcT4dL2KdJryLVVmqlJoEUqUYEzBKpdA0H2mkGo3SDKgxqUI5AqbyTBmKkGcmTXUpJEienJKbre_KtSGO74raeo1VJRsMmQoKwDiloYiA3m5R7VrvHZpi5Wwd_hOgYtNssW82sFc7217VWO7J3yoDcL0FvqR7R7cHpsvJ1qJYlSZQl_9Suy0_au6JSw</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Komatani-Tamiya, Nobuyo</creator><creator>Daikoku, Eriko</creator><creator>Takemura, Yoshizumi</creator><creator>Shimamoto, Chikao</creator><creator>Nakano, Takashi</creator><creator>Iwasaki, Yoshinobu</creator><creator>Kohda, Yuka</creator><creator>Matsumura, Hitoshi</creator><creator>Marunaka, Yoshinori</creator><creator>Nakahari, Takashi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120101</creationdate><title>Procaterol-stimulated Increases in Ciliary Bend Amplitude and Ciliary Beat Frequency in Mouse Bronchioles</title><author>Komatani-Tamiya, Nobuyo ; Daikoku, Eriko ; Takemura, Yoshizumi ; Shimamoto, Chikao ; Nakano, Takashi ; Iwasaki, Yoshinobu ; Kohda, Yuka ; Matsumura, Hitoshi ; Marunaka, Yoshinori ; Nakahari, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-bddb41f304bb228064a8c196ce1cefbc201ff4bea602b97bf1e097f44cd8a0a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adrenergic beta-2 Receptor Agonists - pharmacology</topic><topic>Albuterol - pharmacology</topic><topic>Animals</topic><topic>Axoneme - metabolism</topic><topic>Axoneme - physiology</topic><topic>Bronchioles - drug effects</topic><topic>Bronchioles - metabolism</topic><topic>Bronchioles - physiology</topic><topic>Calcium - pharmacology</topic><topic>Cilia - drug effects</topic><topic>Cilia - metabolism</topic><topic>Cilia - physiology</topic><topic>Cyclic AMP - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - metabolism</topic><topic>Epithelium - physiology</topic><topic>Female</topic><topic>In Vitro Techniques</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microspheres</topic><topic>Mucociliary Clearance</topic><topic>Original</topic><topic>Procaterol - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Komatani-Tamiya, Nobuyo</creatorcontrib><creatorcontrib>Daikoku, Eriko</creatorcontrib><creatorcontrib>Takemura, Yoshizumi</creatorcontrib><creatorcontrib>Shimamoto, Chikao</creatorcontrib><creatorcontrib>Nakano, Takashi</creatorcontrib><creatorcontrib>Iwasaki, Yoshinobu</creatorcontrib><creatorcontrib>Kohda, Yuka</creatorcontrib><creatorcontrib>Matsumura, Hitoshi</creatorcontrib><creatorcontrib>Marunaka, Yoshinori</creatorcontrib><creatorcontrib>Nakahari, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Komatani-Tamiya, Nobuyo</au><au>Daikoku, Eriko</au><au>Takemura, Yoshizumi</au><au>Shimamoto, Chikao</au><au>Nakano, Takashi</au><au>Iwasaki, Yoshinobu</au><au>Kohda, Yuka</au><au>Matsumura, Hitoshi</au><au>Marunaka, Yoshinori</au><au>Nakahari, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Procaterol-stimulated Increases in Ciliary Bend Amplitude and Ciliary Beat Frequency in Mouse Bronchioles</atitle><jtitle>Cellular physiology and biochemistry</jtitle><addtitle>Cell Physiol Biochem</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>29</volume><issue>3-4</issue><spage>511</spage><epage>522</epage><pages>511-522</pages><issn>1015-8987</issn><eissn>1421-9778</eissn><abstract>The beating cilia play a key role in lung mucociliary transport. The ciliary beating frequency (CBF) and ciliary bend amplitude (CBA) of isolated mouse bronchiolar ciliary cells were measured using a light microscope equipped with a high-speed camera (500 Hz). Procaterol (aβ 2 -agonist) increased CBA and CBF in a dose dependent manner via cAMP. The time course of CBA increase is distinct from that of CBF increase: procaterol at 10 nM first increased CBA and then CBF. Moreover, 10 pM procaterol increased CBA, not CBF, whereas 10 nM procaterol increased both CBA and CBF. Concentration-response studies of procaterol demonstrated that the CBA curve was shifted to a lower concentration than the CBF curve, which suggests that CBA regulation is different from CBF regulation. Measurements of microbead movements on the bronchiole of lung slices revealed that 10 pM procaterol increased the rate of ciliary transport by 37% and 10 nM procaterol increased it by 70%. In conclusion, we have shown that increased CBA is of particular importance for increasing the bronchiolar ciliary transport rate, although CBF also plays a role in increasing it.</abstract><cop>Basel, Switzerland</cop><pmid>22508058</pmid><doi>10.1159/000338505</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1015-8987 |
| ispartof | Cellular physiology and biochemistry, 2012-01, Vol.29 (3-4), p.511-522 |
| issn | 1015-8987 1421-9778 |
| language | eng |
| recordid | cdi_pubmed_primary_22508058 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adrenergic beta-2 Receptor Agonists - pharmacology Albuterol - pharmacology Animals Axoneme - metabolism Axoneme - physiology Bronchioles - drug effects Bronchioles - metabolism Bronchioles - physiology Calcium - pharmacology Cilia - drug effects Cilia - metabolism Cilia - physiology Cyclic AMP - metabolism Dose-Response Relationship, Drug Epithelium - drug effects Epithelium - metabolism Epithelium - physiology Female In Vitro Techniques Mice Mice, Inbred C57BL Microspheres Mucociliary Clearance Original Procaterol - pharmacology Time Factors |
| title | Procaterol-stimulated Increases in Ciliary Bend Amplitude and Ciliary Beat Frequency in Mouse Bronchioles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T02%3A50%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Procaterol-stimulated%20Increases%20in%20Ciliary%20Bend%20Amplitude%20and%20Ciliary%20Beat%20Frequency%20in%20Mouse%20Bronchioles&rft.jtitle=Cellular%20physiology%20and%20biochemistry&rft.au=Komatani-Tamiya,%20Nobuyo&rft.date=2012-01-01&rft.volume=29&rft.issue=3-4&rft.spage=511&rft.epage=522&rft.pages=511-522&rft.issn=1015-8987&rft.eissn=1421-9778&rft_id=info:doi/10.1159/000338505&rft_dat=%3Cproquest_pubme%3E1002511792%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1002511792&rft_id=info:pmid/22508058&rfr_iscdi=true |