Dynamics of AHL mediated quorum sensing under flow and non-flow conditions

Quorum sensing (QS) describes the capability of microbes to communicate with each other by the aid of small molecules. Here we investigate the dynamics of QS-regulated gene expression induced by acylhomoserine lactones (AHLs) in Pseudomonas putida IsoF containing a green fluorescent protein-based AH...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Physical biology 2012-04, Vol.9 (2), p.026007-026007
Hauptverfasser: Meyer, Andrea, Megerle, Judith A, Kuttler, Christina, Müller, Johannes, Aguilar, Claudio, Eberl, Leo, Hense, Burkhard A, Rädler, Joachim O
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 026007
container_issue 2
container_start_page 026007
container_title Physical biology
container_volume 9
creator Meyer, Andrea
Megerle, Judith A
Kuttler, Christina
Müller, Johannes
Aguilar, Claudio
Eberl, Leo
Hense, Burkhard A
Rädler, Joachim O
description Quorum sensing (QS) describes the capability of microbes to communicate with each other by the aid of small molecules. Here we investigate the dynamics of QS-regulated gene expression induced by acylhomoserine lactones (AHLs) in Pseudomonas putida IsoF containing a green fluorescent protein-based AHL reporter. The fluorescence time course of individual colonies is monitored following the external addition of a defined AHL concentration to cells which had previously reached the QS-inactive state in AHL-free medium. Using a microfluidic setup the experiment is performed both under flow and non-flow conditions. We find that without supplying external AHL gene expression is induced without flow while flow suppresses the induction. Both without and with flow, at a low AHL concentration the fluorescence onset is significantly delayed while fluorescence starts to increase directly upon the addition of AHL at a high concentration. The differences between no flow and flow can be accounted for using a two-compartment model. This indicates AHL accumulation in a volume which is not affected by the flow. The experiments furthermore show significant cell-to-cell and colony-to-colony variability which is discussed in the context of a compartmentalized QS mechanism.
doi_str_mv 10.1088/1478-3975/9/2/026007
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_22476057</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1011180719</sourcerecordid><originalsourceid>FETCH-LOGICAL-c348t-73e0e0ac5f338192f4dbc1ab1607cdbb9186eff6d86b99bb911ad1bb9a4e018b3</originalsourceid><addsrcrecordid>eNp9kMlOwzAQQC0EoqXwBwj5yCXEdtLYPlZlKagSFzhbjhfkKrFbuxHi70lIqThxmkVvZjQPgGuM7jBiLMclZVnB6TznOckRqRCiJ2B6bJ_-ySfgIqUNQoQTRM_BhJCSVmhOp-Dl_svL1qkEg4WL1Rq2Rju5NxruuhC7Fibjk_MfsPPaRGib8Aml19AHn_0UKnjt9i74dAnOrGySuTrEGXh_fHhbrrL169PzcrHOVFGyfUYLgwySam6LgmFObKlrhWWNK0SVrmuOWWWsrTSras6HGkuN-yhLgzCrixm4HfduY9h1Ju1F65IyTSO9CV0SGGGMGaKY92g5oiqGlKKxYhtdK-NXD4nBohgUiUGR4IKI0WI_dnO40NW9j-PQr7YeQCPgwlZsQhd9__D_O78BuQV72Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1011180719</pqid></control><display><type>article</type><title>Dynamics of AHL mediated quorum sensing under flow and non-flow conditions</title><source>MEDLINE</source><source>Institute of Physics Journals</source><creator>Meyer, Andrea ; Megerle, Judith A ; Kuttler, Christina ; Müller, Johannes ; Aguilar, Claudio ; Eberl, Leo ; Hense, Burkhard A ; Rädler, Joachim O</creator><creatorcontrib>Meyer, Andrea ; Megerle, Judith A ; Kuttler, Christina ; Müller, Johannes ; Aguilar, Claudio ; Eberl, Leo ; Hense, Burkhard A ; Rädler, Joachim O</creatorcontrib><description>Quorum sensing (QS) describes the capability of microbes to communicate with each other by the aid of small molecules. Here we investigate the dynamics of QS-regulated gene expression induced by acylhomoserine lactones (AHLs) in Pseudomonas putida IsoF containing a green fluorescent protein-based AHL reporter. The fluorescence time course of individual colonies is monitored following the external addition of a defined AHL concentration to cells which had previously reached the QS-inactive state in AHL-free medium. Using a microfluidic setup the experiment is performed both under flow and non-flow conditions. We find that without supplying external AHL gene expression is induced without flow while flow suppresses the induction. Both without and with flow, at a low AHL concentration the fluorescence onset is significantly delayed while fluorescence starts to increase directly upon the addition of AHL at a high concentration. The differences between no flow and flow can be accounted for using a two-compartment model. This indicates AHL accumulation in a volume which is not affected by the flow. The experiments furthermore show significant cell-to-cell and colony-to-colony variability which is discussed in the context of a compartmentalized QS mechanism.</description><identifier>ISSN: 1478-3975</identifier><identifier>ISSN: 1478-3967</identifier><identifier>EISSN: 1478-3975</identifier><identifier>DOI: 10.1088/1478-3975/9/2/026007</identifier><identifier>PMID: 22476057</identifier><identifier>CODEN: PBHIAT</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Acyl-Butyrolactones - metabolism ; autoinducer ; cell-to-cell variation ; Dose-Response Relationship, Drug ; gene expression kinetics ; Gene Expression Regulation, Bacterial ; Green Fluorescent Proteins - metabolism ; Ligases - metabolism ; Models, Biological ; Pseudomonas putida - metabolism ; quantitative model ; Quorum Sensing ; single-cell analysis ; Transcriptional Activation</subject><ispartof>Physical biology, 2012-04, Vol.9 (2), p.026007-026007</ispartof><rights>2012 IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-73e0e0ac5f338192f4dbc1ab1607cdbb9186eff6d86b99bb911ad1bb9a4e018b3</citedby><cites>FETCH-LOGICAL-c348t-73e0e0ac5f338192f4dbc1ab1607cdbb9186eff6d86b99bb911ad1bb9a4e018b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/1478-3975/9/2/026007/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>314,780,784,27922,27923,53844,53891</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22476057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Meyer, Andrea</creatorcontrib><creatorcontrib>Megerle, Judith A</creatorcontrib><creatorcontrib>Kuttler, Christina</creatorcontrib><creatorcontrib>Müller, Johannes</creatorcontrib><creatorcontrib>Aguilar, Claudio</creatorcontrib><creatorcontrib>Eberl, Leo</creatorcontrib><creatorcontrib>Hense, Burkhard A</creatorcontrib><creatorcontrib>Rädler, Joachim O</creatorcontrib><title>Dynamics of AHL mediated quorum sensing under flow and non-flow conditions</title><title>Physical biology</title><addtitle>PhysBio</addtitle><addtitle>Phys. Biol</addtitle><description>Quorum sensing (QS) describes the capability of microbes to communicate with each other by the aid of small molecules. Here we investigate the dynamics of QS-regulated gene expression induced by acylhomoserine lactones (AHLs) in Pseudomonas putida IsoF containing a green fluorescent protein-based AHL reporter. The fluorescence time course of individual colonies is monitored following the external addition of a defined AHL concentration to cells which had previously reached the QS-inactive state in AHL-free medium. Using a microfluidic setup the experiment is performed both under flow and non-flow conditions. We find that without supplying external AHL gene expression is induced without flow while flow suppresses the induction. Both without and with flow, at a low AHL concentration the fluorescence onset is significantly delayed while fluorescence starts to increase directly upon the addition of AHL at a high concentration. The differences between no flow and flow can be accounted for using a two-compartment model. This indicates AHL accumulation in a volume which is not affected by the flow. The experiments furthermore show significant cell-to-cell and colony-to-colony variability which is discussed in the context of a compartmentalized QS mechanism.</description><subject>Acyl-Butyrolactones - metabolism</subject><subject>autoinducer</subject><subject>cell-to-cell variation</subject><subject>Dose-Response Relationship, Drug</subject><subject>gene expression kinetics</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Ligases - metabolism</subject><subject>Models, Biological</subject><subject>Pseudomonas putida - metabolism</subject><subject>quantitative model</subject><subject>Quorum Sensing</subject><subject>single-cell analysis</subject><subject>Transcriptional Activation</subject><issn>1478-3975</issn><issn>1478-3967</issn><issn>1478-3975</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQQC0EoqXwBwj5yCXEdtLYPlZlKagSFzhbjhfkKrFbuxHi70lIqThxmkVvZjQPgGuM7jBiLMclZVnB6TznOckRqRCiJ2B6bJ_-ySfgIqUNQoQTRM_BhJCSVmhOp-Dl_svL1qkEg4WL1Rq2Rju5NxruuhC7Fibjk_MfsPPaRGib8Aml19AHn_0UKnjt9i74dAnOrGySuTrEGXh_fHhbrrL169PzcrHOVFGyfUYLgwySam6LgmFObKlrhWWNK0SVrmuOWWWsrTSras6HGkuN-yhLgzCrixm4HfduY9h1Ju1F65IyTSO9CV0SGGGMGaKY92g5oiqGlKKxYhtdK-NXD4nBohgUiUGR4IKI0WI_dnO40NW9j-PQr7YeQCPgwlZsQhd9__D_O78BuQV72Q</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Meyer, Andrea</creator><creator>Megerle, Judith A</creator><creator>Kuttler, Christina</creator><creator>Müller, Johannes</creator><creator>Aguilar, Claudio</creator><creator>Eberl, Leo</creator><creator>Hense, Burkhard A</creator><creator>Rädler, Joachim O</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120401</creationdate><title>Dynamics of AHL mediated quorum sensing under flow and non-flow conditions</title><author>Meyer, Andrea ; Megerle, Judith A ; Kuttler, Christina ; Müller, Johannes ; Aguilar, Claudio ; Eberl, Leo ; Hense, Burkhard A ; Rädler, Joachim O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-73e0e0ac5f338192f4dbc1ab1607cdbb9186eff6d86b99bb911ad1bb9a4e018b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acyl-Butyrolactones - metabolism</topic><topic>autoinducer</topic><topic>cell-to-cell variation</topic><topic>Dose-Response Relationship, Drug</topic><topic>gene expression kinetics</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Ligases - metabolism</topic><topic>Models, Biological</topic><topic>Pseudomonas putida - metabolism</topic><topic>quantitative model</topic><topic>Quorum Sensing</topic><topic>single-cell analysis</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meyer, Andrea</creatorcontrib><creatorcontrib>Megerle, Judith A</creatorcontrib><creatorcontrib>Kuttler, Christina</creatorcontrib><creatorcontrib>Müller, Johannes</creatorcontrib><creatorcontrib>Aguilar, Claudio</creatorcontrib><creatorcontrib>Eberl, Leo</creatorcontrib><creatorcontrib>Hense, Burkhard A</creatorcontrib><creatorcontrib>Rädler, Joachim O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Physical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meyer, Andrea</au><au>Megerle, Judith A</au><au>Kuttler, Christina</au><au>Müller, Johannes</au><au>Aguilar, Claudio</au><au>Eberl, Leo</au><au>Hense, Burkhard A</au><au>Rädler, Joachim O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamics of AHL mediated quorum sensing under flow and non-flow conditions</atitle><jtitle>Physical biology</jtitle><stitle>PhysBio</stitle><addtitle>Phys. Biol</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>9</volume><issue>2</issue><spage>026007</spage><epage>026007</epage><pages>026007-026007</pages><issn>1478-3975</issn><issn>1478-3967</issn><eissn>1478-3975</eissn><coden>PBHIAT</coden><abstract>Quorum sensing (QS) describes the capability of microbes to communicate with each other by the aid of small molecules. Here we investigate the dynamics of QS-regulated gene expression induced by acylhomoserine lactones (AHLs) in Pseudomonas putida IsoF containing a green fluorescent protein-based AHL reporter. The fluorescence time course of individual colonies is monitored following the external addition of a defined AHL concentration to cells which had previously reached the QS-inactive state in AHL-free medium. Using a microfluidic setup the experiment is performed both under flow and non-flow conditions. We find that without supplying external AHL gene expression is induced without flow while flow suppresses the induction. Both without and with flow, at a low AHL concentration the fluorescence onset is significantly delayed while fluorescence starts to increase directly upon the addition of AHL at a high concentration. The differences between no flow and flow can be accounted for using a two-compartment model. This indicates AHL accumulation in a volume which is not affected by the flow. The experiments furthermore show significant cell-to-cell and colony-to-colony variability which is discussed in the context of a compartmentalized QS mechanism.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>22476057</pmid><doi>10.1088/1478-3975/9/2/026007</doi><tpages>10</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1478-3975
ispartof Physical biology, 2012-04, Vol.9 (2), p.026007-026007
issn 1478-3975
1478-3967
1478-3975
language eng
recordid cdi_pubmed_primary_22476057
source MEDLINE; Institute of Physics Journals
subjects Acyl-Butyrolactones - metabolism
autoinducer
cell-to-cell variation
Dose-Response Relationship, Drug
gene expression kinetics
Gene Expression Regulation, Bacterial
Green Fluorescent Proteins - metabolism
Ligases - metabolism
Models, Biological
Pseudomonas putida - metabolism
quantitative model
Quorum Sensing
single-cell analysis
Transcriptional Activation
title Dynamics of AHL mediated quorum sensing under flow and non-flow conditions
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T04%3A53%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dynamics%20of%20AHL%20mediated%20quorum%20sensing%20under%20flow%20and%20non-flow%20conditions&rft.jtitle=Physical%20biology&rft.au=Meyer,%20Andrea&rft.date=2012-04-01&rft.volume=9&rft.issue=2&rft.spage=026007&rft.epage=026007&rft.pages=026007-026007&rft.issn=1478-3975&rft.eissn=1478-3975&rft.coden=PBHIAT&rft_id=info:doi/10.1088/1478-3975/9/2/026007&rft_dat=%3Cproquest_pubme%3E1011180719%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1011180719&rft_id=info:pmid/22476057&rfr_iscdi=true