Heterologous Expression of Manganese Peroxidase in Aspergillus niger and Its Effect on Phenanthrene Removal from Soil
A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degra...
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| Veröffentlicht in: | Journal of molecular microbiology and biotechnology 2011-01, Vol.21 (3-4), p.120-129 |
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| container_title | Journal of molecular microbiology and biotechnology |
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| creator | Cortés-Espinosa, Diana V. Absalón, Ángel E. Sanchez, Noé Loera, Octavio Rodríguez-Vázquez, Refugio Fernández, Francisco J. |
| description | A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain. |
| doi_str_mv | 10.1159/000331563 |
| format | Article |
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Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.</description><identifier>ISSN: 2673-1665</identifier><identifier>ISSN: 1464-1801</identifier><identifier>EISSN: 2673-1673</identifier><identifier>EISSN: 1660-2412</identifier><identifier>DOI: 10.1159/000331563</identifier><identifier>PMID: 22286039</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Aspergillus niger - enzymology ; Aspergillus niger - genetics ; Aspergillus niger - metabolism ; Bagasse ; Biotransformation ; Discoloration ; DNA Primers - genetics ; DNA, Fungal - genetics ; Manganese ; Peroxidases - genetics ; Peroxidases - metabolism ; Phanerochaete - enzymology ; Phanerochaete - genetics ; Phenanthrene ; Phenanthrenes - metabolism ; Polycyclic aromatic hydrocarbons ; Polymerase Chain Reaction ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Research Article ; Selection, Genetic ; Soil Pollutants - metabolism ; Sugarcane ; Time Factors ; Transformation, Genetic</subject><ispartof>Journal of molecular microbiology and biotechnology, 2011-01, Vol.21 (3-4), p.120-129</ispartof><rights>2012 S. Karger AG, Basel</rights><rights>Copyright © 2012 S. Karger AG, Basel.</rights><rights>Copyright (c) 2012 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-6e0adffbde3a6bb8b3036d24e793cda2a652a573acfc3070687811f3d6f205dd3</citedby><cites>FETCH-LOGICAL-c399t-6e0adffbde3a6bb8b3036d24e793cda2a652a573acfc3070687811f3d6f205dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22286039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cortés-Espinosa, Diana V.</creatorcontrib><creatorcontrib>Absalón, Ángel E.</creatorcontrib><creatorcontrib>Sanchez, Noé</creatorcontrib><creatorcontrib>Loera, Octavio</creatorcontrib><creatorcontrib>Rodríguez-Vázquez, Refugio</creatorcontrib><creatorcontrib>Fernández, Francisco J.</creatorcontrib><title>Heterologous Expression of Manganese Peroxidase in Aspergillus niger and Its Effect on Phenanthrene Removal from Soil</title><title>Journal of molecular microbiology and biotechnology</title><addtitle>Microb Physiol</addtitle><description>A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.</description><subject>Aspergillus niger - enzymology</subject><subject>Aspergillus niger - genetics</subject><subject>Aspergillus niger - metabolism</subject><subject>Bagasse</subject><subject>Biotransformation</subject><subject>Discoloration</subject><subject>DNA Primers - genetics</subject><subject>DNA, Fungal - genetics</subject><subject>Manganese</subject><subject>Peroxidases - genetics</subject><subject>Peroxidases - metabolism</subject><subject>Phanerochaete - enzymology</subject><subject>Phanerochaete - genetics</subject><subject>Phenanthrene</subject><subject>Phenanthrenes - metabolism</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Polymerase Chain Reaction</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Research Article</subject><subject>Selection, Genetic</subject><subject>Soil Pollutants - metabolism</subject><subject>Sugarcane</subject><subject>Time Factors</subject><subject>Transformation, Genetic</subject><issn>2673-1665</issn><issn>1464-1801</issn><issn>2673-1673</issn><issn>1660-2412</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0T1PwzAQBmALgQABAztClhgQQ8H2NU4yVlWhlUAgPubIic8lJbGLnaDy7zFq6cBie3jek--OkFPOrjlP8hvGGABPJOyQQyFTGPB47G7fMjkgJyEsIhNDLrI02ScHQohMMsgPST_FDr1r3Nz1gU5WS48h1M5SZ-iDsnNlMSB9imRVaxWftaWjsEQ_r5smJmw9R0-V1XTWxbwxWHU0xp_e0SrbvXu0SJ-xdV-qoca7lr64ujkme0Y1AU829xF5u528jqeD-8e72Xh0P6ggz7uBRKa0MaVGULIssxIYSC2GmOZQaSWUTIRKUlCVqYClTGZpxrkBLY1gidZwRC7XdZfeffYYuqKtQ4VNE9uK_RY5z3IYypxHefFPLlzvbfxcwRkwASlwEdXVWlXeheDRFEtft8p_R1T8bqPYbiPa803FvmxRb-Xf7CM4W4MP5eMQt2CT_wH7co3i</recordid><startdate>20110101</startdate><enddate>20110101</enddate><creator>Cortés-Espinosa, Diana V.</creator><creator>Absalón, Ángel E.</creator><creator>Sanchez, Noé</creator><creator>Loera, Octavio</creator><creator>Rodríguez-Vázquez, Refugio</creator><creator>Fernández, Francisco J.</creator><general>S. 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enzymology</topic><topic>Aspergillus niger - genetics</topic><topic>Aspergillus niger - metabolism</topic><topic>Bagasse</topic><topic>Biotransformation</topic><topic>Discoloration</topic><topic>DNA Primers - genetics</topic><topic>DNA, Fungal - genetics</topic><topic>Manganese</topic><topic>Peroxidases - genetics</topic><topic>Peroxidases - metabolism</topic><topic>Phanerochaete - enzymology</topic><topic>Phanerochaete - genetics</topic><topic>Phenanthrene</topic><topic>Phenanthrenes - metabolism</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Polymerase Chain Reaction</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Research Article</topic><topic>Selection, Genetic</topic><topic>Soil Pollutants - metabolism</topic><topic>Sugarcane</topic><topic>Time Factors</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cortés-Espinosa, Diana V.</creatorcontrib><creatorcontrib>Absalón, Ángel E.</creatorcontrib><creatorcontrib>Sanchez, Noé</creatorcontrib><creatorcontrib>Loera, Octavio</creatorcontrib><creatorcontrib>Rodríguez-Vázquez, Refugio</creatorcontrib><creatorcontrib>Fernández, Francisco J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cortés-Espinosa, Diana V.</au><au>Absalón, Ángel E.</au><au>Sanchez, Noé</au><au>Loera, Octavio</au><au>Rodríguez-Vázquez, Refugio</au><au>Fernández, Francisco J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous Expression of Manganese Peroxidase in Aspergillus niger and Its Effect on Phenanthrene Removal from Soil</atitle><jtitle>Journal of molecular microbiology and biotechnology</jtitle><addtitle>Microb Physiol</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>21</volume><issue>3-4</issue><spage>120</spage><epage>129</epage><pages>120-129</pages><issn>2673-1665</issn><issn>1464-1801</issn><eissn>2673-1673</eissn><eissn>1660-2412</eissn><abstract>A strain of Aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from Phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. Transformants were selected based on their resistance to hygromycin B and the discoloration induced on Poly R-478 dye by the peroxidase activity. The recombinant A. niger SBC2-T3 strain developed MnP activity and was able to remove 95% of the initial phenanthrene (400 ppm) from a microcosm soil system after 17 days, whereas the wild strain removed 72% under the same conditions. Transformation success was confirmed by PCR amplification using gene-specific primers, and a single fragment (1,348 bp long, as expected) of the recombinant mnp1 was amplified in the DNA from transformants, which was absent from the parental strain.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>22286039</pmid><doi>10.1159/000331563</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 2673-1665 |
| ispartof | Journal of molecular microbiology and biotechnology, 2011-01, Vol.21 (3-4), p.120-129 |
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| language | eng |
| recordid | cdi_pubmed_primary_22286039 |
| source | MEDLINE; Karger Journals; Alma/SFX Local Collection |
| subjects | Aspergillus niger - enzymology Aspergillus niger - genetics Aspergillus niger - metabolism Bagasse Biotransformation Discoloration DNA Primers - genetics DNA, Fungal - genetics Manganese Peroxidases - genetics Peroxidases - metabolism Phanerochaete - enzymology Phanerochaete - genetics Phenanthrene Phenanthrenes - metabolism Polycyclic aromatic hydrocarbons Polymerase Chain Reaction Recombinant Proteins - genetics Recombinant Proteins - metabolism Research Article Selection, Genetic Soil Pollutants - metabolism Sugarcane Time Factors Transformation, Genetic |
| title | Heterologous Expression of Manganese Peroxidase in Aspergillus niger and Its Effect on Phenanthrene Removal from Soil |
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