Trypanocide, cytotoxic, and antifungal activities of Momordica charantia
Context: Chagas disease, caused by Trypanosoma cruzi, is a public health problem. Currently, chemotherapy is the only available treatment for this disease, and the drugs used, nifurtimox and benzonidazol, present high toxicity levels. An alternative for replacing these drugs are natural extracts fro...
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| Veröffentlicht in: | Pharmaceutical biology 2012-02, Vol.50 (2), p.162-166 |
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| creator | Santos, Karla K.A. Matias, Edinardo F.F. Sobral-Souza, Celestina E. Tintino, Saulo R. Morais-Braga, Maria F.B. Guedes, Glaucia M.M. Santos, Francisco A.V. A. Sousa, Ana Carla Rolón, Miriam Vega, Celeste de Arias, Antonieta Rojas Costa, José G.M. Menezes, Irwin R.A. Coutinho, Henrique D.M. |
| description | Context: Chagas disease, caused by Trypanosoma cruzi, is a public health problem. Currently, chemotherapy is the only available treatment for this disease, and the drugs used, nifurtimox and benzonidazol, present high toxicity levels. An alternative for replacing these drugs are natural extracts from Momordica charantia L. (Cucurbitaceae) used in traditional medicine because of their antimicrobial and biological activities.
Objective: In this study, we evaluated the extract of M. charantia for its antiepimastigote, antifungal, and cytotoxic activities.
Materials and methods: An ethanol extract of leaves from M. charantia was prepared. To research in vitro antiepimastigote activity, T. cruzi CL-B5 clone was used. Epimastigotes were inoculated at a concentration of 1 × 105 cells/mL in 200 µl tryptose-liver infusion. For the cytotoxicity assay, J774 macrophages were used. The antifungal activity was evaluated by microdilution using strains of Candida albicans, Candida tropicalis, and Candida krusei.
Results: The effective concentration capable of killing 50% of parasites (IC50) was 46.06 µg/mL. The minimum inhibitory concentration (MIC) was ≤ 1024 µg/mL. Metronidazole showed a potentiation of its antifungal effect when combined with an extract of M. charantia.
Conclusions: Our results indicate that M. charantia could be a source of plant-derived natural products with antiepimastigote and antifungal-modifying activity with moderate toxicity. |
| doi_str_mv | 10.3109/13880209.2011.581672 |
| format | Article |
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Objective: In this study, we evaluated the extract of M. charantia for its antiepimastigote, antifungal, and cytotoxic activities.
Materials and methods: An ethanol extract of leaves from M. charantia was prepared. To research in vitro antiepimastigote activity, T. cruzi CL-B5 clone was used. Epimastigotes were inoculated at a concentration of 1 × 105 cells/mL in 200 µl tryptose-liver infusion. For the cytotoxicity assay, J774 macrophages were used. The antifungal activity was evaluated by microdilution using strains of Candida albicans, Candida tropicalis, and Candida krusei.
Results: The effective concentration capable of killing 50% of parasites (IC50) was 46.06 µg/mL. The minimum inhibitory concentration (MIC) was ≤ 1024 µg/mL. Metronidazole showed a potentiation of its antifungal effect when combined with an extract of M. charantia.
Conclusions: Our results indicate that M. charantia could be a source of plant-derived natural products with antiepimastigote and antifungal-modifying activity with moderate toxicity.</description><identifier>ISSN: 1388-0209</identifier><identifier>EISSN: 1744-5116</identifier><identifier>DOI: 10.3109/13880209.2011.581672</identifier><identifier>PMID: 22235885</identifier><language>eng</language><publisher>England: Informa Healthcare</publisher><subject>Animals ; antiepimastigote activity ; Antifungal Agents - isolation & purification ; Antifungal Agents - pharmacology ; Antifungal Agents - toxicity ; Candida - drug effects ; Candida spp ; Cell Line ; Chagas disease ; Drug Synergism ; Inhibitory Concentration 50 ; Macrophages - drug effects ; Macrophages - metabolism ; Medicine, Traditional ; Metronidazole - administration & dosage ; Metronidazole - pharmacology ; Mice ; Microbial Sensitivity Tests ; modifying activity ; Momordica charantia - chemistry ; Parasitic Sensitivity Tests ; Plant Extracts - administration & dosage ; Plant Extracts - pharmacology ; Plant Extracts - toxicity ; Plant Leaves ; Toxicity Tests ; Trypanocidal Agents - isolation & purification ; Trypanocidal Agents - pharmacology ; Trypanocidal Agents - toxicity ; Trypanosoma cruzi - drug effects</subject><ispartof>Pharmaceutical biology, 2012-02, Vol.50 (2), p.162-166</ispartof><rights>2012 Informa Healthcare USA, Inc. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e8ee8a30e1958089a2ab642f35c3363779f3a68131d1f7575f8c6fd66cb4938c3</citedby><cites>FETCH-LOGICAL-c417t-e8ee8a30e1958089a2ab642f35c3363779f3a68131d1f7575f8c6fd66cb4938c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22235885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santos, Karla K.A.</creatorcontrib><creatorcontrib>Matias, Edinardo F.F.</creatorcontrib><creatorcontrib>Sobral-Souza, Celestina E.</creatorcontrib><creatorcontrib>Tintino, Saulo R.</creatorcontrib><creatorcontrib>Morais-Braga, Maria F.B.</creatorcontrib><creatorcontrib>Guedes, Glaucia M.M.</creatorcontrib><creatorcontrib>Santos, Francisco A.V.</creatorcontrib><creatorcontrib>A. Sousa, Ana Carla</creatorcontrib><creatorcontrib>Rolón, Miriam</creatorcontrib><creatorcontrib>Vega, Celeste</creatorcontrib><creatorcontrib>de Arias, Antonieta Rojas</creatorcontrib><creatorcontrib>Costa, José G.M.</creatorcontrib><creatorcontrib>Menezes, Irwin R.A.</creatorcontrib><creatorcontrib>Coutinho, Henrique D.M.</creatorcontrib><title>Trypanocide, cytotoxic, and antifungal activities of Momordica charantia</title><title>Pharmaceutical biology</title><addtitle>Pharm Biol</addtitle><description>Context: Chagas disease, caused by Trypanosoma cruzi, is a public health problem. Currently, chemotherapy is the only available treatment for this disease, and the drugs used, nifurtimox and benzonidazol, present high toxicity levels. An alternative for replacing these drugs are natural extracts from Momordica charantia L. (Cucurbitaceae) used in traditional medicine because of their antimicrobial and biological activities.
Objective: In this study, we evaluated the extract of M. charantia for its antiepimastigote, antifungal, and cytotoxic activities.
Materials and methods: An ethanol extract of leaves from M. charantia was prepared. To research in vitro antiepimastigote activity, T. cruzi CL-B5 clone was used. Epimastigotes were inoculated at a concentration of 1 × 105 cells/mL in 200 µl tryptose-liver infusion. For the cytotoxicity assay, J774 macrophages were used. The antifungal activity was evaluated by microdilution using strains of Candida albicans, Candida tropicalis, and Candida krusei.
Results: The effective concentration capable of killing 50% of parasites (IC50) was 46.06 µg/mL. The minimum inhibitory concentration (MIC) was ≤ 1024 µg/mL. Metronidazole showed a potentiation of its antifungal effect when combined with an extract of M. charantia.
Conclusions: Our results indicate that M. charantia could be a source of plant-derived natural products with antiepimastigote and antifungal-modifying activity with moderate toxicity.</description><subject>Animals</subject><subject>antiepimastigote activity</subject><subject>Antifungal Agents - isolation & purification</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antifungal Agents - toxicity</subject><subject>Candida - drug effects</subject><subject>Candida spp</subject><subject>Cell Line</subject><subject>Chagas disease</subject><subject>Drug Synergism</subject><subject>Inhibitory Concentration 50</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - metabolism</subject><subject>Medicine, Traditional</subject><subject>Metronidazole - administration & dosage</subject><subject>Metronidazole - pharmacology</subject><subject>Mice</subject><subject>Microbial Sensitivity Tests</subject><subject>modifying activity</subject><subject>Momordica charantia - chemistry</subject><subject>Parasitic Sensitivity Tests</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - toxicity</subject><subject>Plant Leaves</subject><subject>Toxicity Tests</subject><subject>Trypanocidal Agents - isolation & purification</subject><subject>Trypanocidal Agents - pharmacology</subject><subject>Trypanocidal Agents - toxicity</subject><subject>Trypanosoma cruzi - drug effects</subject><issn>1388-0209</issn><issn>1744-5116</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAUhoMobk7_gUh_wDrz0aTpjSJDnTDxZl6HszRxGW0z0k7tv7elTvBmF-Hk4nnfkzwIXRM8YwRnt4RJiSnOZhQTMuOSiJSeoDFJkyTmhIjT7t4hcc-M0EVdbzHGnDF-jkaUUsal5GO0WIV2B5XXLjfTSLeNb_y309MIqrw7jbP76gOKCHTjPl3jTB15G7360ofcaYj0BkKPwSU6s1DU5up3TtD70-NqvoiXb88v84dlrBOSNrGRxkhg2JCMSywzoLAWCbWMa8YES9PMMhCSMJITm_KUW6mFzYXQ6yRjUrMJSoZeHXxdB2PVLrgSQqsIVr0YdRCjejFqENPFbobYbr8uTf4XOpjogPsBcJX1oYQvH4pcNdAWPtjui9rVff3RFXf_GjYGimajIRi19ftQdVaOv_EHKG6FXw</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Santos, Karla K.A.</creator><creator>Matias, Edinardo F.F.</creator><creator>Sobral-Souza, Celestina E.</creator><creator>Tintino, Saulo R.</creator><creator>Morais-Braga, Maria F.B.</creator><creator>Guedes, Glaucia M.M.</creator><creator>Santos, Francisco A.V.</creator><creator>A. Sousa, Ana Carla</creator><creator>Rolón, Miriam</creator><creator>Vega, Celeste</creator><creator>de Arias, Antonieta Rojas</creator><creator>Costa, José G.M.</creator><creator>Menezes, Irwin R.A.</creator><creator>Coutinho, Henrique D.M.</creator><general>Informa Healthcare</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201202</creationdate><title>Trypanocide, cytotoxic, and antifungal activities of Momordica charantia</title><author>Santos, Karla K.A. ; Matias, Edinardo F.F. ; Sobral-Souza, Celestina E. ; Tintino, Saulo R. ; Morais-Braga, Maria F.B. ; Guedes, Glaucia M.M. ; Santos, Francisco A.V. ; A. 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Sousa, Ana Carla</creatorcontrib><creatorcontrib>Rolón, Miriam</creatorcontrib><creatorcontrib>Vega, Celeste</creatorcontrib><creatorcontrib>de Arias, Antonieta Rojas</creatorcontrib><creatorcontrib>Costa, José G.M.</creatorcontrib><creatorcontrib>Menezes, Irwin R.A.</creatorcontrib><creatorcontrib>Coutinho, Henrique D.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Pharmaceutical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos, Karla K.A.</au><au>Matias, Edinardo F.F.</au><au>Sobral-Souza, Celestina E.</au><au>Tintino, Saulo R.</au><au>Morais-Braga, Maria F.B.</au><au>Guedes, Glaucia M.M.</au><au>Santos, Francisco A.V.</au><au>A. Sousa, Ana Carla</au><au>Rolón, Miriam</au><au>Vega, Celeste</au><au>de Arias, Antonieta Rojas</au><au>Costa, José G.M.</au><au>Menezes, Irwin R.A.</au><au>Coutinho, Henrique D.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trypanocide, cytotoxic, and antifungal activities of Momordica charantia</atitle><jtitle>Pharmaceutical biology</jtitle><addtitle>Pharm Biol</addtitle><date>2012-02</date><risdate>2012</risdate><volume>50</volume><issue>2</issue><spage>162</spage><epage>166</epage><pages>162-166</pages><issn>1388-0209</issn><eissn>1744-5116</eissn><abstract>Context: Chagas disease, caused by Trypanosoma cruzi, is a public health problem. Currently, chemotherapy is the only available treatment for this disease, and the drugs used, nifurtimox and benzonidazol, present high toxicity levels. An alternative for replacing these drugs are natural extracts from Momordica charantia L. (Cucurbitaceae) used in traditional medicine because of their antimicrobial and biological activities.
Objective: In this study, we evaluated the extract of M. charantia for its antiepimastigote, antifungal, and cytotoxic activities.
Materials and methods: An ethanol extract of leaves from M. charantia was prepared. To research in vitro antiepimastigote activity, T. cruzi CL-B5 clone was used. Epimastigotes were inoculated at a concentration of 1 × 105 cells/mL in 200 µl tryptose-liver infusion. For the cytotoxicity assay, J774 macrophages were used. The antifungal activity was evaluated by microdilution using strains of Candida albicans, Candida tropicalis, and Candida krusei.
Results: The effective concentration capable of killing 50% of parasites (IC50) was 46.06 µg/mL. The minimum inhibitory concentration (MIC) was ≤ 1024 µg/mL. Metronidazole showed a potentiation of its antifungal effect when combined with an extract of M. charantia.
Conclusions: Our results indicate that M. charantia could be a source of plant-derived natural products with antiepimastigote and antifungal-modifying activity with moderate toxicity.</abstract><cop>England</cop><pub>Informa Healthcare</pub><pmid>22235885</pmid><doi>10.3109/13880209.2011.581672</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals antiepimastigote activity Antifungal Agents - isolation & purification Antifungal Agents - pharmacology Antifungal Agents - toxicity Candida - drug effects Candida spp Cell Line Chagas disease Drug Synergism Inhibitory Concentration 50 Macrophages - drug effects Macrophages - metabolism Medicine, Traditional Metronidazole - administration & dosage Metronidazole - pharmacology Mice Microbial Sensitivity Tests modifying activity Momordica charantia - chemistry Parasitic Sensitivity Tests Plant Extracts - administration & dosage Plant Extracts - pharmacology Plant Extracts - toxicity Plant Leaves Toxicity Tests Trypanocidal Agents - isolation & purification Trypanocidal Agents - pharmacology Trypanocidal Agents - toxicity Trypanosoma cruzi - drug effects |
| title | Trypanocide, cytotoxic, and antifungal activities of Momordica charantia |
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