Vacuolar Glyphosate-Sequestration Correlates with Glyphosate Resistance in Ryegrass (Lolium spp.) from Australia, South America, and Europe: A 31P NMR Investigation
Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by 31P NMR. Extents of glyphosate (i) resistance (LD50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC50), and (iii) transloc...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2012-02, Vol.60 (5), p.1243-1250 |
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| creator | Ge, Xia d’Avignon, D. André Ackerman, Joseph J. H Collavo, Alberto Sattin, Maurizio Ostrander, Elizabeth L Hall, Erin L Sammons, R. Douglas Preston, Christopher |
| description | Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by 31P NMR. Extents of glyphosate (i) resistance (LD50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC50), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD50 and IC50 data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by 31P NMR. All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous 31P NMR studies of horseweed (Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well. |
| doi_str_mv | 10.1021/jf203472s |
| format | Article |
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André ; Ackerman, Joseph J. H ; Collavo, Alberto ; Sattin, Maurizio ; Ostrander, Elizabeth L ; Hall, Erin L ; Sammons, R. Douglas ; Preston, Christopher</creator><creatorcontrib>Ge, Xia ; d’Avignon, D. André ; Ackerman, Joseph J. H ; Collavo, Alberto ; Sattin, Maurizio ; Ostrander, Elizabeth L ; Hall, Erin L ; Sammons, R. Douglas ; Preston, Christopher</creatorcontrib><description>Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by 31P NMR. Extents of glyphosate (i) resistance (LD50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC50), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD50 and IC50 data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by 31P NMR. All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous 31P NMR studies of horseweed (Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf203472s</identifier><identifier>PMID: 22224711</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Australia ; Biological and medical sciences ; Biological Transport ; Conyza canadensis ; Europe ; Food industries ; Fundamental and applied biological sciences. Psychology ; Glycine - analogs & derivatives ; Glycine - metabolism ; Glycine - pharmacology ; Glyphosate ; Herbicide Resistance ; Herbicides - metabolism ; Herbicides - pharmacology ; inhibitory concentration 50 ; lethal dose 50 ; Lolium - chemistry ; Lolium - drug effects ; Lolium - metabolism ; Lolium multiflorum ; Lolium rigidum ; Magnetic Resonance Spectroscopy ; nuclear magnetic resonance spectroscopy ; South America ; vacuoles ; Vacuoles - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2012-02, Vol.60 (5), p.1243-1250</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf203472s$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf203472s$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25625848$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22224711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Xia</creatorcontrib><creatorcontrib>d’Avignon, D. André</creatorcontrib><creatorcontrib>Ackerman, Joseph J. H</creatorcontrib><creatorcontrib>Collavo, Alberto</creatorcontrib><creatorcontrib>Sattin, Maurizio</creatorcontrib><creatorcontrib>Ostrander, Elizabeth L</creatorcontrib><creatorcontrib>Hall, Erin L</creatorcontrib><creatorcontrib>Sammons, R. Douglas</creatorcontrib><creatorcontrib>Preston, Christopher</creatorcontrib><title>Vacuolar Glyphosate-Sequestration Correlates with Glyphosate Resistance in Ryegrass (Lolium spp.) from Australia, South America, and Europe: A 31P NMR Investigation</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by 31P NMR. Extents of glyphosate (i) resistance (LD50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC50), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD50 and IC50 data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by 31P NMR. All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous 31P NMR studies of horseweed (Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well.</description><subject>Australia</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Conyza canadensis</subject><subject>Europe</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycine - analogs & derivatives</subject><subject>Glycine - metabolism</subject><subject>Glycine - pharmacology</subject><subject>Glyphosate</subject><subject>Herbicide Resistance</subject><subject>Herbicides - metabolism</subject><subject>Herbicides - pharmacology</subject><subject>inhibitory concentration 50</subject><subject>lethal dose 50</subject><subject>Lolium - chemistry</subject><subject>Lolium - drug effects</subject><subject>Lolium - metabolism</subject><subject>Lolium multiflorum</subject><subject>Lolium rigidum</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>South America</subject><subject>vacuoles</subject><subject>Vacuoles - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkd1u1DAQhS0EokvhghcA3yCBRIrHsdcOd6tVaSstP9ql3EaT7GTrVRKndgLa9-FBcX-gzIVHY386OsfD2EsQJyAkfNg3UuTKyPiIzUBLkWkA-5jNRHrMrJ7DEXsW414IYbURT9mRTKUMwIz9_oH15FsM_Kw9DFc-4kjZhq4nimPA0fmeL30I1Kb7yH-58eo_kK8pujhiXxN3PV8faBcwRv525Vs3dTwOw8k73gTf8cV0o9c6fM83fkoqi46Cq9OI_ZafTsEP9JEveA7f-JfPa37R_0wO3O7WwnP2pME20ov7fswuP51-X55nq69nF8vFKkPIC8ikwhqUQJ0OQ4XRUBVKo55bQiCbV8pUORUkDcLWommwsoXemlqhVmR1fsxe3ekOU9XRthyC6zAcyr_flYA39wDGGtsmpOguPnB6LrVVNnGv77gGfYm7kJjLjRTJlwDQc50_KGEdy72fQp-ClSDKm4WW_xaa_wEuGY6m</recordid><startdate>20120208</startdate><enddate>20120208</enddate><creator>Ge, Xia</creator><creator>d’Avignon, D. André</creator><creator>Ackerman, Joseph J. H</creator><creator>Collavo, Alberto</creator><creator>Sattin, Maurizio</creator><creator>Ostrander, Elizabeth L</creator><creator>Hall, Erin L</creator><creator>Sammons, R. Douglas</creator><creator>Preston, Christopher</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20120208</creationdate><title>Vacuolar Glyphosate-Sequestration Correlates with Glyphosate Resistance in Ryegrass (Lolium spp.) from Australia, South America, and Europe: A 31P NMR Investigation</title><author>Ge, Xia ; d’Avignon, D. André ; Ackerman, Joseph J. H ; Collavo, Alberto ; Sattin, Maurizio ; Ostrander, Elizabeth L ; Hall, Erin L ; Sammons, R. Douglas ; Preston, Christopher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a1391-24ac140a51407e9751b945a568ea1e83b47b3e9e27a1d8a7fab895d7c4a54e853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Australia</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Conyza canadensis</topic><topic>Europe</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycine - analogs & derivatives</topic><topic>Glycine - metabolism</topic><topic>Glycine - pharmacology</topic><topic>Glyphosate</topic><topic>Herbicide Resistance</topic><topic>Herbicides - metabolism</topic><topic>Herbicides - pharmacology</topic><topic>inhibitory concentration 50</topic><topic>lethal dose 50</topic><topic>Lolium - chemistry</topic><topic>Lolium - drug effects</topic><topic>Lolium - metabolism</topic><topic>Lolium multiflorum</topic><topic>Lolium rigidum</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>South America</topic><topic>vacuoles</topic><topic>Vacuoles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Xia</creatorcontrib><creatorcontrib>d’Avignon, D. André</creatorcontrib><creatorcontrib>Ackerman, Joseph J. H</creatorcontrib><creatorcontrib>Collavo, Alberto</creatorcontrib><creatorcontrib>Sattin, Maurizio</creatorcontrib><creatorcontrib>Ostrander, Elizabeth L</creatorcontrib><creatorcontrib>Hall, Erin L</creatorcontrib><creatorcontrib>Sammons, R. Douglas</creatorcontrib><creatorcontrib>Preston, Christopher</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Xia</au><au>d’Avignon, D. André</au><au>Ackerman, Joseph J. H</au><au>Collavo, Alberto</au><au>Sattin, Maurizio</au><au>Ostrander, Elizabeth L</au><au>Hall, Erin L</au><au>Sammons, R. Douglas</au><au>Preston, Christopher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vacuolar Glyphosate-Sequestration Correlates with Glyphosate Resistance in Ryegrass (Lolium spp.) from Australia, South America, and Europe: A 31P NMR Investigation</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2012-02-08</date><risdate>2012</risdate><volume>60</volume><issue>5</issue><spage>1243</spage><epage>1250</epage><pages>1243-1250</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Lolium spp., ryegrass, variants from Australia, Brazil, Chile, and Italy showing differing levels of glyphosate resistance were examined by 31P NMR. Extents of glyphosate (i) resistance (LD50), (ii) inhibition of 5-enopyruvyl-shikimate-3-phosphate synthase (EPSPS) activity (IC50), and (iii) translocation were quantified for glyphosate-resistant (GR) and glyphosate-sensitive (GS) Lolium multiflorum Lam. variants from Chile and Brazil. For comparison, LD50 and IC50 data for Lolium rigidum Gaudin variants from Italy were also analyzed. All variants showed similar cellular uptake of glyphosate by 31P NMR. All GR variants showed glyphosate sequestration within the cell vacuole, whereas there was minimal or no vacuole sequestration in the GS variants. The extent of vacuole sequestration correlated qualitatively with the level of resistance. Previous 31P NMR studies of horseweed (Conyza canadensis (L.) Cronquist) revealed that glyphosate sequestration imparted glyphosate resistance. Data presented herein suggest that glyphosate vacuolar sequestration is strongly contributing, if not the major contributing, resistance mechanism in ryegrass as well.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>22224711</pmid><doi>10.1021/jf203472s</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; ACS Publications |
| subjects | Australia Biological and medical sciences Biological Transport Conyza canadensis Europe Food industries Fundamental and applied biological sciences. Psychology Glycine - analogs & derivatives Glycine - metabolism Glycine - pharmacology Glyphosate Herbicide Resistance Herbicides - metabolism Herbicides - pharmacology inhibitory concentration 50 lethal dose 50 Lolium - chemistry Lolium - drug effects Lolium - metabolism Lolium multiflorum Lolium rigidum Magnetic Resonance Spectroscopy nuclear magnetic resonance spectroscopy South America vacuoles Vacuoles - metabolism |
| title | Vacuolar Glyphosate-Sequestration Correlates with Glyphosate Resistance in Ryegrass (Lolium spp.) from Australia, South America, and Europe: A 31P NMR Investigation |
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