identification, characterization, sequencing and mutagenesis of the genes (hupSL) encoding the small and large subunits of the H2-uptake hydrogenase of Azotobacter chroococcum

Summary The structural genes (hupSL) of the membrane‐bound NiFe‐containing H2‐uptake hydrogenase (Hup) of Azotobacter chroococcum were identified by oligo‐nucleotide screening and sequenced. The small subunit gene (hupS) encodes a signal sequence of 34 amino acids followed by a 310‐amino‐acid, 34 15...

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