Dopaminergic Stimulation of Cyclic AMP Accumulation and Parathyroid Hormone Release from Dispersed Bovine Parathyroid Cells
The effects of dopaminergic agonists and antagonists have been studied in dispersed bovine parathyroid cells. Dopaminergic agonists caused a transient 20- to 40-fold increase in cellular cyclic AMP and a 2- to 3-fold increase in parathyroid hormone release. Dose-response relationships were similar f...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1977-10, Vol.74 (10), p.4210-4213 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4213 |
|---|---|
| container_issue | 10 |
| container_start_page | 4210 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 74 |
| creator | Brown, E. M. Carroll, R. J. Aurbach, G. D. |
| description | The effects of dopaminergic agonists and antagonists have been studied in dispersed bovine parathyroid cells. Dopaminergic agonists caused a transient 20- to 40-fold increase in cellular cyclic AMP and a 2- to 3-fold increase in parathyroid hormone release. Dose-response relationships were similar for cyclic AMP accumulation and hormone release, whether studied by increasing agonist concentration or by increasing concentration of antagonist with constant agonist. The effects on the dopamine receptor could be differentiated from those of the previously characterized β -adrenergic receptor by specific inhibitors. These results appear to represent proof with a homogeneous cell population that dopaminergic receptors linked to adenylate cyclase can regulate a secretory process mediated by cyclic AMP. This system should be useful in further studies on dopamine receptors and should provide a valid tool for determining interactions of radiolabeled ligands with such receptors. |
| doi_str_mv | 10.1073/pnas.74.10.4210 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmed_primary_22076</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>67704</jstor_id><sourcerecordid>67704</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-4a70c64b3e64eaf2b49b5f9888b1c40cde772281a6d4e24d9e2da6a7129500323</originalsourceid><addsrcrecordid>eNp9kU1v1DAURS1EBUNhjQQS8oquMn12nDhZdDFMaYtURMXH2nKcl9ZVEgfbqRjx50k6w4jZsLLse46frUvIawZLBjI9HXodllJMm6XgDJ6QBYOSJbko4SlZAHCZFIKL5-RFCPcAUGYFPCNHnIPMF-T3uRt0Z3v0t9bQb9F2Y6ujdT11DV1vTDudrj7f0JUx4z7SfU1vtNfxbuOdremV853rkX7FFnVA2njX0XMbBvQBa_rBPUwDDow1tm14SY4a3QZ8tVuPyY-Lj9_XV8n1l8tP69V1YkRWxkRoCSYXVYq5QN3wSpRV1pRFUVTMCDA1Ssl5wXReC-SiLpHXOteS8TIDSHl6TM629w5j1WFtsI9et2rwttN-o5y26jDp7Z26dQ9KpKyEYvLf73zvfo4YoupsMNMPdI9uDEqmMksLOYOnW9B4F4LHZj-DgZrLUnNZSop5P5c1GW__fdqef2xnSt_t0ln7mx3oJ_8FVDO2bcRfcSLfbMn7EJ3fo7mUINI_Lfaz8w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73753878</pqid></control><display><type>article</type><title>Dopaminergic Stimulation of Cyclic AMP Accumulation and Parathyroid Hormone Release from Dispersed Bovine Parathyroid Cells</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Brown, E. M. ; Carroll, R. J. ; Aurbach, G. D.</creator><creatorcontrib>Brown, E. M. ; Carroll, R. J. ; Aurbach, G. D.</creatorcontrib><description>The effects of dopaminergic agonists and antagonists have been studied in dispersed bovine parathyroid cells. Dopaminergic agonists caused a transient 20- to 40-fold increase in cellular cyclic AMP and a 2- to 3-fold increase in parathyroid hormone release. Dose-response relationships were similar for cyclic AMP accumulation and hormone release, whether studied by increasing agonist concentration or by increasing concentration of antagonist with constant agonist. The effects on the dopamine receptor could be differentiated from those of the previously characterized β -adrenergic receptor by specific inhibitors. These results appear to represent proof with a homogeneous cell population that dopaminergic receptors linked to adenylate cyclase can regulate a secretory process mediated by cyclic AMP. This system should be useful in further studies on dopamine receptors and should provide a valid tool for determining interactions of radiolabeled ligands with such receptors.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.74.10.4210</identifier><identifier>PMID: 22076</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Adrenergic beta-Agonists - pharmacology ; Agonists ; Animals ; B lymphocytes ; Biological Sciences: Biochemistry ; Cattle ; Cell separation ; Cyclic AMP - metabolism ; Dopamine - pharmacology ; Dopamine - physiology ; Dopamine agonists ; Dopamine Antagonists ; Dopamine receptors ; Female ; Hormones ; Inhibitory concentration 50 ; Neurons ; Parathyroid Glands - drug effects ; Parathyroid Glands - metabolism ; Parathyroid Hormone - metabolism ; Receptors ; Time Factors ; Ungulates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1977-10, Vol.74 (10), p.4210-4213</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-4a70c64b3e64eaf2b49b5f9888b1c40cde772281a6d4e24d9e2da6a7129500323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/74/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/67704$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/67704$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, E. M.</creatorcontrib><creatorcontrib>Carroll, R. J.</creatorcontrib><creatorcontrib>Aurbach, G. D.</creatorcontrib><title>Dopaminergic Stimulation of Cyclic AMP Accumulation and Parathyroid Hormone Release from Dispersed Bovine Parathyroid Cells</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The effects of dopaminergic agonists and antagonists have been studied in dispersed bovine parathyroid cells. Dopaminergic agonists caused a transient 20- to 40-fold increase in cellular cyclic AMP and a 2- to 3-fold increase in parathyroid hormone release. Dose-response relationships were similar for cyclic AMP accumulation and hormone release, whether studied by increasing agonist concentration or by increasing concentration of antagonist with constant agonist. The effects on the dopamine receptor could be differentiated from those of the previously characterized β -adrenergic receptor by specific inhibitors. These results appear to represent proof with a homogeneous cell population that dopaminergic receptors linked to adenylate cyclase can regulate a secretory process mediated by cyclic AMP. This system should be useful in further studies on dopamine receptors and should provide a valid tool for determining interactions of radiolabeled ligands with such receptors.</description><subject>Adrenergic beta-Agonists - pharmacology</subject><subject>Agonists</subject><subject>Animals</subject><subject>B lymphocytes</subject><subject>Biological Sciences: Biochemistry</subject><subject>Cattle</subject><subject>Cell separation</subject><subject>Cyclic AMP - metabolism</subject><subject>Dopamine - pharmacology</subject><subject>Dopamine - physiology</subject><subject>Dopamine agonists</subject><subject>Dopamine Antagonists</subject><subject>Dopamine receptors</subject><subject>Female</subject><subject>Hormones</subject><subject>Inhibitory concentration 50</subject><subject>Neurons</subject><subject>Parathyroid Glands - drug effects</subject><subject>Parathyroid Glands - metabolism</subject><subject>Parathyroid Hormone - metabolism</subject><subject>Receptors</subject><subject>Time Factors</subject><subject>Ungulates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAURS1EBUNhjQQS8oquMn12nDhZdDFMaYtURMXH2nKcl9ZVEgfbqRjx50k6w4jZsLLse46frUvIawZLBjI9HXodllJMm6XgDJ6QBYOSJbko4SlZAHCZFIKL5-RFCPcAUGYFPCNHnIPMF-T3uRt0Z3v0t9bQb9F2Y6ujdT11DV1vTDudrj7f0JUx4z7SfU1vtNfxbuOdremV853rkX7FFnVA2njX0XMbBvQBa_rBPUwDDow1tm14SY4a3QZ8tVuPyY-Lj9_XV8n1l8tP69V1YkRWxkRoCSYXVYq5QN3wSpRV1pRFUVTMCDA1Ssl5wXReC-SiLpHXOteS8TIDSHl6TM629w5j1WFtsI9et2rwttN-o5y26jDp7Z26dQ9KpKyEYvLf73zvfo4YoupsMNMPdI9uDEqmMksLOYOnW9B4F4LHZj-DgZrLUnNZSop5P5c1GW__fdqef2xnSt_t0ln7mx3oJ_8FVDO2bcRfcSLfbMn7EJ3fo7mUINI_Lfaz8w</recordid><startdate>19771001</startdate><enddate>19771001</enddate><creator>Brown, E. M.</creator><creator>Carroll, R. J.</creator><creator>Aurbach, G. D.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19771001</creationdate><title>Dopaminergic Stimulation of Cyclic AMP Accumulation and Parathyroid Hormone Release from Dispersed Bovine Parathyroid Cells</title><author>Brown, E. M. ; Carroll, R. J. ; Aurbach, G. D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-4a70c64b3e64eaf2b49b5f9888b1c40cde772281a6d4e24d9e2da6a7129500323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Adrenergic beta-Agonists - pharmacology</topic><topic>Agonists</topic><topic>Animals</topic><topic>B lymphocytes</topic><topic>Biological Sciences: Biochemistry</topic><topic>Cattle</topic><topic>Cell separation</topic><topic>Cyclic AMP - metabolism</topic><topic>Dopamine - pharmacology</topic><topic>Dopamine - physiology</topic><topic>Dopamine agonists</topic><topic>Dopamine Antagonists</topic><topic>Dopamine receptors</topic><topic>Female</topic><topic>Hormones</topic><topic>Inhibitory concentration 50</topic><topic>Neurons</topic><topic>Parathyroid Glands - drug effects</topic><topic>Parathyroid Glands - metabolism</topic><topic>Parathyroid Hormone - metabolism</topic><topic>Receptors</topic><topic>Time Factors</topic><topic>Ungulates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, E. M.</creatorcontrib><creatorcontrib>Carroll, R. J.</creatorcontrib><creatorcontrib>Aurbach, G. D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, E. M.</au><au>Carroll, R. J.</au><au>Aurbach, G. D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dopaminergic Stimulation of Cyclic AMP Accumulation and Parathyroid Hormone Release from Dispersed Bovine Parathyroid Cells</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1977-10-01</date><risdate>1977</risdate><volume>74</volume><issue>10</issue><spage>4210</spage><epage>4213</epage><pages>4210-4213</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The effects of dopaminergic agonists and antagonists have been studied in dispersed bovine parathyroid cells. Dopaminergic agonists caused a transient 20- to 40-fold increase in cellular cyclic AMP and a 2- to 3-fold increase in parathyroid hormone release. Dose-response relationships were similar for cyclic AMP accumulation and hormone release, whether studied by increasing agonist concentration or by increasing concentration of antagonist with constant agonist. The effects on the dopamine receptor could be differentiated from those of the previously characterized β -adrenergic receptor by specific inhibitors. These results appear to represent proof with a homogeneous cell population that dopaminergic receptors linked to adenylate cyclase can regulate a secretory process mediated by cyclic AMP. This system should be useful in further studies on dopamine receptors and should provide a valid tool for determining interactions of radiolabeled ligands with such receptors.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>22076</pmid><doi>10.1073/pnas.74.10.4210</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1977-10, Vol.74 (10), p.4210-4213 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_22076 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adrenergic beta-Agonists - pharmacology Agonists Animals B lymphocytes Biological Sciences: Biochemistry Cattle Cell separation Cyclic AMP - metabolism Dopamine - pharmacology Dopamine - physiology Dopamine agonists Dopamine Antagonists Dopamine receptors Female Hormones Inhibitory concentration 50 Neurons Parathyroid Glands - drug effects Parathyroid Glands - metabolism Parathyroid Hormone - metabolism Receptors Time Factors Ungulates |
| title | Dopaminergic Stimulation of Cyclic AMP Accumulation and Parathyroid Hormone Release from Dispersed Bovine Parathyroid Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T01%3A16%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dopaminergic%20Stimulation%20of%20Cyclic%20AMP%20Accumulation%20and%20Parathyroid%20Hormone%20Release%20from%20Dispersed%20Bovine%20Parathyroid%20Cells&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Brown,%20E.%20M.&rft.date=1977-10-01&rft.volume=74&rft.issue=10&rft.spage=4210&rft.epage=4213&rft.pages=4210-4213&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.74.10.4210&rft_dat=%3Cjstor_pubme%3E67704%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73753878&rft_id=info:pmid/22076&rft_jstor_id=67704&rfr_iscdi=true |