Detection of stx and stx genes in Pennsylvanian white-tailed deer
Shiga toxin-producing E. coli carrying the stx(1) and/or stx(2) genes can cause multi-symptomatic illness in humans. A variety of terrestrial and aquatic environmental reservoirs of stx have been described. Culture based detection of microbes in deer species have found a low percentage of samples th...
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| Veröffentlicht in: | Toxins 2011-06, Vol.3 (6), p.640 |
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| creator | Kistler, Whitney M Mulugeta, Surafel Mauro, Steven A |
| description | Shiga toxin-producing E. coli carrying the stx(1) and/or stx(2) genes can cause multi-symptomatic illness in humans. A variety of terrestrial and aquatic environmental reservoirs of stx have been described. Culture based detection of microbes in deer species have found a low percentage of samples that have tested positive for Stx-producing microbes, suggesting that while deer may contain these microbes, their overall abundance in deer is low. In this study, quantitative PCR (qPCR) was utilized to test for the presence of stx genes in white-tailed deer fecal matter in western Pennsylvania. In this culture independent screening, nearly half of the samples tested positive for the stx(2) gene, with a bias towards samples that were concentrated with stx(2). This study, while limited in scope, suggests that deer may be a greater reservoir for stx than was previously thought. |
| doi_str_mv | 10.3390/toxins3060640 |
| format | Article |
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| ispartof | Toxins, 2011-06, Vol.3 (6), p.640 |
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| language | eng |
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| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Animals Deer - microbiology Disease Reservoirs DNA, Bacterial - genetics Environmental Microbiology Feces - chemistry Feces - microbiology Pennsylvania Real-Time Polymerase Chain Reaction Shiga Toxin - genetics Shiga Toxin - isolation & purification Shiga-Toxigenic Escherichia coli - isolation & purification Shiga-Toxigenic Escherichia coli - metabolism |
| title | Detection of stx and stx genes in Pennsylvanian white-tailed deer |
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