Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH)
The power of chemical synthesis of large cysteine-free polypeptides has been significantly enhanced through the use of nonproteogenic constructs which bear strategically placed thiol groups, enabling native chemical ligation. Central to these much expanded capabilities is the specific, radical-induc...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2011-04, Vol.108 (15), p.5986-5989 |
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| container_issue | 15 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 108 |
| creator | Shang, Shiying Tan, Zhongping Danishefsky, Samuel J |
| description | The power of chemical synthesis of large cysteine-free polypeptides has been significantly enhanced through the use of nonproteogenic constructs which bear strategically placed thiol groups, enabling native chemical ligation. Central to these much expanded capabilities is the specific, radical-induced, metal-free dethiolation, which can be accomplished in aqueous medium. |
| doi_str_mv | 10.1073/pnas.1103118108 |
| format | Article |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2011-04, Vol.108 (15), p.5986-5989 |
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| source | MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; JSTOR |
| subjects | Amino Acid Sequence Amino Acid Substitution Amino acids Antimony Aqueous solutions Biochemistry Chemical synthesis Chemicals Circular Dichroism Cysteine - chemistry Hormones Humans Ligation Molecular Sequence Data parathyroid hormone Parathyroid Hormone - chemical synthesis Parathyroid Hormone - chemistry Peptides Pharmaceutical chemistry Physical Sciences Polypeptides Proteins Solvents synthesis thiols |
| title | Application of the logic of cysteine-free native chemical ligation to the synthesis of Human Parathyroid Hormone (hPTH) |
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