Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm
Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil mic...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1990-02, Vol.56 (2), p.401-408 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Trevors, J.T. (University of Guelph, Guelph, Ontario, Canada) Elsas, J.D. van Overbeek, L.S. van Starodub, M.E |
| description | Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed with groundwater. The presence of wheat roots growing downward in the microcosms contributed only slightly to the movement of P. fluorescens C5t cells to lower soil regions of the loamy sand microcosms, but enhanced downward transport in the loam microcosms. Furthermore, the introduced P. fluorescens C5t cells were detected in the effluent water samples even after three flushes of groundwater and 10 days of incubation. As evidenced by a comparison of counts from immunofluorescence and selective plating, nonculturable C5t cells occurred in day 10 soil and percolated water samples, primarily of the loamy sand microcosms. Vertical soil microcosms that use water movement may be useful in studying the survival and transport of genetically engineered bacteria in soil under a variety of conditions prior to field testing |
| doi_str_mv | 10.1128/aem.56.2.401-408.1990 |
| format | Article |
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(University of Guelph, Guelph, Ontario, Canada) ; Elsas, J.D. van ; Overbeek, L.S. van ; Starodub, M.E</creator><creatorcontrib>Trevors, J.T. (University of Guelph, Guelph, Ontario, Canada) ; Elsas, J.D. van ; Overbeek, L.S. van ; Starodub, M.E</creatorcontrib><description>Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed with groundwater. The presence of wheat roots growing downward in the microcosms contributed only slightly to the movement of P. fluorescens C5t cells to lower soil regions of the loamy sand microcosms, but enhanced downward transport in the loam microcosms. Furthermore, the introduced P. fluorescens C5t cells were detected in the effluent water samples even after three flushes of groundwater and 10 days of incubation. As evidenced by a comparison of counts from immunofluorescence and selective plating, nonculturable C5t cells occurred in day 10 soil and percolated water samples, primarily of the loamy sand microcosms. Vertical soil microcosms that use water movement may be useful in studying the survival and transport of genetically engineered bacteria in soil under a variety of conditions prior to field testing</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.56.2.401-408.1990</identifier><identifier>PMID: 2106286</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Biological and medical sciences ; Biotechnology ; Colony Count, Microbial ; Environment and pollution ; Fluorescent Antibody Technique ; Fresh Water ; Fundamental and applied biological sciences. Psychology ; Genetic Engineering ; Genetically engineered organisms behavior (microorganisms, plants, animals) ; GENIE GENETIQUE ; ground water ; Industrial applications and implications. Economical aspects ; INGENIERIA GENETICA ; Nucleic Acid Hybridization ; PROCESOS DE TRANSPORTE EN EL SUELO ; PSEUDOMONAS ; Pseudomonas fluorescens - genetics ; Pseudomonas fluorescens - growth & development ; Pseudomonas fluorescens - physiology ; Soil Microbiology ; soils (loam) ; SOL SABLEUX ; SOL SABLOLIMONEUX ; SUELO ARENOSO ; SUELO FRANCO ; SUPERVIVENCIA ; SURVIE ; TRANSPORT DANS LE SOL ; Triticum ; vertical distribution ; water infiltration ; Water Microbiology</subject><ispartof>Applied and Environmental Microbiology, 1990-02, Vol.56 (2), p.401-408</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c664t-38b494e97ca5986989ad1c64ae6309c42b48297c1e5486a58a7e303dfe0889433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC183352/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC183352/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19400778$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2106286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trevors, J.T. (University of Guelph, Guelph, Ontario, Canada)</creatorcontrib><creatorcontrib>Elsas, J.D. van</creatorcontrib><creatorcontrib>Overbeek, L.S. van</creatorcontrib><creatorcontrib>Starodub, M.E</creatorcontrib><title>Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed with groundwater. The presence of wheat roots growing downward in the microcosms contributed only slightly to the movement of P. fluorescens C5t cells to lower soil regions of the loamy sand microcosms, but enhanced downward transport in the loam microcosms. Furthermore, the introduced P. fluorescens C5t cells were detected in the effluent water samples even after three flushes of groundwater and 10 days of incubation. As evidenced by a comparison of counts from immunofluorescence and selective plating, nonculturable C5t cells occurred in day 10 soil and percolated water samples, primarily of the loamy sand microcosms. Vertical soil microcosms that use water movement may be useful in studying the survival and transport of genetically engineered bacteria in soil under a variety of conditions prior to field testing</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Colony Count, Microbial</subject><subject>Environment and pollution</subject><subject>Fluorescent Antibody Technique</subject><subject>Fresh Water</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Engineering</subject><subject>Genetically engineered organisms behavior (microorganisms, plants, animals)</subject><subject>GENIE GENETIQUE</subject><subject>ground water</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>INGENIERIA GENETICA</subject><subject>Nucleic Acid Hybridization</subject><subject>PROCESOS DE TRANSPORTE EN EL SUELO</subject><subject>PSEUDOMONAS</subject><subject>Pseudomonas fluorescens - genetics</subject><subject>Pseudomonas fluorescens - growth & development</subject><subject>Pseudomonas fluorescens - physiology</subject><subject>Soil Microbiology</subject><subject>soils (loam)</subject><subject>SOL SABLEUX</subject><subject>SOL SABLOLIMONEUX</subject><subject>SUELO ARENOSO</subject><subject>SUELO FRANCO</subject><subject>SUPERVIVENCIA</subject><subject>SURVIE</subject><subject>TRANSPORT DANS LE SOL</subject><subject>Triticum</subject><subject>vertical distribution</subject><subject>water infiltration</subject><subject>Water Microbiology</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktv1TAQhS0EKpfCH0Cq5A3sEsbP2AsWqOIlVQKJdoks32SSGCXxxU5A_fe4uleFrlhYXpzvjGfmmJALBjVj3LzxONdK17yWwCoJpmbWwiOyY2BNpYTQj8kOwNqKcwlPybOcfwCABG3OyBlnoLnRO_L9OvklH2JaaeyppwMuuIbWT9MtxWUIC2LCjn7NuHVxjovPtJ-2mDC3uGSa1-TDQtcxxW0Yiz_HMNE5tCm2Mc_PyZPeTxlfnO5zcvPh_fXlp-rqy8fPl--uqlZruVbC7KWVaJvWK2u0NdZ3rNXSoxZgW8n30vCiMlTSaK-Mb1CA6HoEY6wU4py8PdY9bPsZu9Ja6WtyhxRmn25d9ME9VJYwuiH-cswIoXjxvz75U_y5YV7dHMqA0-QXjFt2jdVSgoL_gkxp1gipC6iOYNlEzgn7-2YYuLv8XMnPKe24K_mVY9xdfsV38e8k965TYEV_ddJ9Lin1Jb025L_FrQRoGlM4euTGMIy_Q0Ln8_zgzYK8PCK9j84PqZS5-WaB6_JJxB9PJrkG</recordid><startdate>19900201</startdate><enddate>19900201</enddate><creator>Trevors, J.T. (University of Guelph, Guelph, Ontario, Canada)</creator><creator>Elsas, J.D. van</creator><creator>Overbeek, L.S. van</creator><creator>Starodub, M.E</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900201</creationdate><title>Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm</title><author>Trevors, J.T. (University of Guelph, Guelph, Ontario, Canada) ; Elsas, J.D. van ; Overbeek, L.S. van ; Starodub, M.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c664t-38b494e97ca5986989ad1c64ae6309c42b48297c1e5486a58a7e303dfe0889433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Colony Count, Microbial</topic><topic>Environment and pollution</topic><topic>Fluorescent Antibody Technique</topic><topic>Fresh Water</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Engineering</topic><topic>Genetically engineered organisms behavior (microorganisms, plants, animals)</topic><topic>GENIE GENETIQUE</topic><topic>ground water</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>INGENIERIA GENETICA</topic><topic>Nucleic Acid Hybridization</topic><topic>PROCESOS DE TRANSPORTE EN EL SUELO</topic><topic>PSEUDOMONAS</topic><topic>Pseudomonas fluorescens - genetics</topic><topic>Pseudomonas fluorescens - growth & development</topic><topic>Pseudomonas fluorescens - physiology</topic><topic>Soil Microbiology</topic><topic>soils (loam)</topic><topic>SOL SABLEUX</topic><topic>SOL SABLOLIMONEUX</topic><topic>SUELO ARENOSO</topic><topic>SUELO FRANCO</topic><topic>SUPERVIVENCIA</topic><topic>SURVIE</topic><topic>TRANSPORT DANS LE SOL</topic><topic>Triticum</topic><topic>vertical distribution</topic><topic>water infiltration</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trevors, J.T. (University of Guelph, Guelph, Ontario, Canada)</creatorcontrib><creatorcontrib>Elsas, J.D. van</creatorcontrib><creatorcontrib>Overbeek, L.S. van</creatorcontrib><creatorcontrib>Starodub, M.E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trevors, J.T. (University of Guelph, Guelph, Ontario, Canada)</au><au>Elsas, J.D. van</au><au>Overbeek, L.S. van</au><au>Starodub, M.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1990-02-01</date><risdate>1990</risdate><volume>56</volume><issue>2</issue><spage>401</spage><epage>408</epage><pages>401-408</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Vertical soil microcosms flushed with groundwater were used to study the influence of water movement on survival and transport of a genetically engineered Pseudomonas fluorescens C5t strain through a loamy sand and a loam soil. Transport of cells introduced into the top 1 cm of the vertical soil microcosms was dependent on the flow rate of water and the number of times microcosms were flushed with groundwater. The presence of wheat roots growing downward in the microcosms contributed only slightly to the movement of P. fluorescens C5t cells to lower soil regions of the loamy sand microcosms, but enhanced downward transport in the loam microcosms. Furthermore, the introduced P. fluorescens C5t cells were detected in the effluent water samples even after three flushes of groundwater and 10 days of incubation. As evidenced by a comparison of counts from immunofluorescence and selective plating, nonculturable C5t cells occurred in day 10 soil and percolated water samples, primarily of the loamy sand microcosms. Vertical soil microcosms that use water movement may be useful in studying the survival and transport of genetically engineered bacteria in soil under a variety of conditions prior to field testing</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>2106286</pmid><doi>10.1128/aem.56.2.401-408.1990</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 1990-02, Vol.56 (2), p.401-408 |
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| language | eng |
| recordid | cdi_pubmed_primary_2106286 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Biological and medical sciences Biotechnology Colony Count, Microbial Environment and pollution Fluorescent Antibody Technique Fresh Water Fundamental and applied biological sciences. Psychology Genetic Engineering Genetically engineered organisms behavior (microorganisms, plants, animals) GENIE GENETIQUE ground water Industrial applications and implications. Economical aspects INGENIERIA GENETICA Nucleic Acid Hybridization PROCESOS DE TRANSPORTE EN EL SUELO PSEUDOMONAS Pseudomonas fluorescens - genetics Pseudomonas fluorescens - growth & development Pseudomonas fluorescens - physiology Soil Microbiology soils (loam) SOL SABLEUX SOL SABLOLIMONEUX SUELO ARENOSO SUELO FRANCO SUPERVIVENCIA SURVIE TRANSPORT DANS LE SOL Triticum vertical distribution water infiltration Water Microbiology |
| title | Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm |
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