The Effect of Tween 80 on Eggshell Permeabilization in Galleria Mellonella (L.) (Lepidoptera, Pyralidae)

The development of a species-specific protocol for dechorionation/permeabilization of insect eggs is a necessary prerequisite to cryopreserve the embryos. Here we tested different procedures based on heptane or the surfactant Tween 80 as an alternative to alkane, evaluating their efficacy and toxici...

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Veröffentlicht in:	Cryo-Letters 2010-07, Vol.31 (4), p.291-300
Hauptverfasser:	Cosi, Elena, Abidalla, Muhamad T., Roversi, Pio Federico
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Membrane Permeability - drug effects Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacokinetics Dechorionation Diverse techniques Eggshell Fundamental and applied biological sciences. Psychology Galleria Mellonella Heptanes - pharmacology Insecta Invertebrates Lepidoptera - embryology Life cycle. Embryology. Development Molecular and cellular biology Ovum - drug effects Ovum - metabolism Permeabilization Physiology. Development Polysorbates - pharmacology Sodium Hypochlorite - pharmacology Surface-Active Agents - pharmacology Thiosulfates - pharmacology Tween 80 Vitelline Membrane - drug effects Vitelline Membrane - metabolism
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