Trojan horse mechanism of bacterial pathogenesis against nematodes
Understanding the mechanisms of host-pathogen interaction can provide crucial information for successfully manipulating their relationships. Because of its genetic background and practical advantages over vertebrate model systems, the nematode Caenorhabditis elegans model has become an attractive ho...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2010-09, Vol.107 (38), p.16631-16636 |
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| container_issue | 38 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 107 |
| creator | Niu, Qiuhong Huang, Xiaowei Zhang, Lin Xu, Jianping Yang, Dongmei Wei, Kangbi Niu, Xuemei An, Zhiqiang Bennett, Joan Wennstrom Zou, Chenggang Yang, Jinkui Zhang, Ke-Qin |
| description | Understanding the mechanisms of host-pathogen interaction can provide crucial information for successfully manipulating their relationships. Because of its genetic background and practical advantages over vertebrate model systems, the nematode Caenorhabditis elegans model has become an attractive host for studying microbial pathogenesis. Here we report a "Trojan horse" mechanism of bacterial pathogenesis against nematodes. We show that the bacterium Bacillus nematocida B16 lures nematodes by emitting potent volatile organic compounds that are much more attractive to worms than those from ordinary dietary bacteria. Seventeen B. nematocida-attractant volatile organic compounds are identified, and seven are individually confirmed to lure nematodes. Once the bacteria enter the intestine of nematodes, they secrete two proteases with broad substrate ranges but preferentially target essential intestinal proteins, leading to nematode death. This Trojan horse pattern of bacterium-nematode interaction enriches our understanding of microbial pathogenesis. |
| doi_str_mv | 10.1073/pnas.1007276107 |
| format | Article |
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Because of its genetic background and practical advantages over vertebrate model systems, the nematode Caenorhabditis elegans model has become an attractive host for studying microbial pathogenesis. Here we report a "Trojan horse" mechanism of bacterial pathogenesis against nematodes. We show that the bacterium Bacillus nematocida B16 lures nematodes by emitting potent volatile organic compounds that are much more attractive to worms than those from ordinary dietary bacteria. Seventeen B. nematocida-attractant volatile organic compounds are identified, and seven are individually confirmed to lure nematodes. Once the bacteria enter the intestine of nematodes, they secrete two proteases with broad substrate ranges but preferentially target essential intestinal proteins, leading to nematode death. This Trojan horse pattern of bacterium-nematode interaction enriches our understanding of microbial pathogenesis.</description><identifier>ISSN: 0027-8424</identifier><identifier>ISSN: 1091-6490</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1007276107</identifier><identifier>PMID: 20733068</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Bacillus ; Bacillus - pathogenicity ; Bacillus - physiology ; Bacteria ; Biological Sciences ; Caenorhabditis elegans ; Caenorhabditis elegans - microbiology ; Caenorhabditis elegans - physiology ; Genetics ; Host-pathogen interactions ; Host-Pathogen Interactions - physiology ; Intestine ; Intestines ; Intestines - microbiology ; Mortality ; Nematoda ; Nematodes ; Odorants ; Pathogenesis ; Pathogens ; Peptide Hydrolases - metabolism ; Proteases ; Proteinase ; Proteins ; Soil - parasitology ; Soil nematodes ; Virulence ; Virulence - physiology ; Virulence Factors - metabolism ; VOCs ; Volatile organic compounds ; Volatile Organic Compounds - metabolism ; Worms</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2010-09, Vol.107 (38), p.16631-16636</ispartof><rights>Copyright National Academy of Sciences Sep 21, 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c555t-d259a4f2a7090e1cf498688767ac2d303497a6f1a7f746701e63abecef9de9823</citedby><cites>FETCH-LOGICAL-c555t-d259a4f2a7090e1cf498688767ac2d303497a6f1a7f746701e63abecef9de9823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/107/38.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20779718$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20779718$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53769,53771,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20733068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niu, Qiuhong</creatorcontrib><creatorcontrib>Huang, Xiaowei</creatorcontrib><creatorcontrib>Zhang, Lin</creatorcontrib><creatorcontrib>Xu, Jianping</creatorcontrib><creatorcontrib>Yang, Dongmei</creatorcontrib><creatorcontrib>Wei, Kangbi</creatorcontrib><creatorcontrib>Niu, Xuemei</creatorcontrib><creatorcontrib>An, Zhiqiang</creatorcontrib><creatorcontrib>Bennett, Joan Wennstrom</creatorcontrib><creatorcontrib>Zou, Chenggang</creatorcontrib><creatorcontrib>Yang, Jinkui</creatorcontrib><creatorcontrib>Zhang, Ke-Qin</creatorcontrib><title>Trojan horse mechanism of bacterial pathogenesis against nematodes</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Understanding the mechanisms of host-pathogen interaction can provide crucial information for successfully manipulating their relationships. Because of its genetic background and practical advantages over vertebrate model systems, the nematode Caenorhabditis elegans model has become an attractive host for studying microbial pathogenesis. Here we report a "Trojan horse" mechanism of bacterial pathogenesis against nematodes. We show that the bacterium Bacillus nematocida B16 lures nematodes by emitting potent volatile organic compounds that are much more attractive to worms than those from ordinary dietary bacteria. Seventeen B. nematocida-attractant volatile organic compounds are identified, and seven are individually confirmed to lure nematodes. Once the bacteria enter the intestine of nematodes, they secrete two proteases with broad substrate ranges but preferentially target essential intestinal proteins, leading to nematode death. This Trojan horse pattern of bacterium-nematode interaction enriches our understanding of microbial pathogenesis.</description><subject>Animals</subject><subject>Bacillus</subject><subject>Bacillus - pathogenicity</subject><subject>Bacillus - physiology</subject><subject>Bacteria</subject><subject>Biological Sciences</subject><subject>Caenorhabditis elegans</subject><subject>Caenorhabditis elegans - microbiology</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Genetics</subject><subject>Host-pathogen interactions</subject><subject>Host-Pathogen Interactions - physiology</subject><subject>Intestine</subject><subject>Intestines</subject><subject>Intestines - microbiology</subject><subject>Mortality</subject><subject>Nematoda</subject><subject>Nematodes</subject><subject>Odorants</subject><subject>Pathogenesis</subject><subject>Pathogens</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Proteases</subject><subject>Proteinase</subject><subject>Proteins</subject><subject>Soil - parasitology</subject><subject>Soil nematodes</subject><subject>Virulence</subject><subject>Virulence - physiology</subject><subject>Virulence Factors - metabolism</subject><subject>VOCs</subject><subject>Volatile organic compounds</subject><subject>Volatile Organic Compounds - metabolism</subject><subject>Worms</subject><issn>0027-8424</issn><issn>1091-6490</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhzAmIuMAl1HZsj31BohVfUiUOtGdrNpnsZrWxFzuLxL-vo13awgFOtjzPPPLMy9hzwd8JDs3ZLmAuNw4STHl4wBaCO1Eb5fhDtuBcQm2VVCfsSc4bzrnTlj9mJ7L0NtzYBTu_SnGDoVrHlKkaqV1jGPJYxb5aYjtRGnBb7XBaxxUFykOucIVDyFMVaMQpdpSfskc9bjM9O56n7PrTx6uLL_Xlt89fLz5c1q3Weqo7qR2qXiJwx0m0vXLWWAsGsJVdwxvlAE0vEHpQBrgg0-CSWupdR87K5pS9P3h3--VIXUthSrj1uzSMmH75iIP_sxKGtV_Fn146pYqvCN4cBSn-2FOe_DjklrZbDBT32VttQIMw9r8kaC2cdXp2vv0nKaQGa0AaU9DXf6GbuE-hrGz2cSVAuwKdHaA2xZwT9bfzCe7nyP0cub-LvHS8vL-WW_53xgWojsDceacD31gvysfmKV4ckE2eYrqvAAdiVrw61HuMHldpyP76uywr5cI6KUzT3ACiZ8RP</recordid><startdate>20100921</startdate><enddate>20100921</enddate><creator>Niu, Qiuhong</creator><creator>Huang, Xiaowei</creator><creator>Zhang, Lin</creator><creator>Xu, Jianping</creator><creator>Yang, Dongmei</creator><creator>Wei, Kangbi</creator><creator>Niu, Xuemei</creator><creator>An, Zhiqiang</creator><creator>Bennett, Joan Wennstrom</creator><creator>Zou, Chenggang</creator><creator>Yang, Jinkui</creator><creator>Zhang, Ke-Qin</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7T7</scope><scope>5PM</scope></search><sort><creationdate>20100921</creationdate><title>Trojan horse mechanism of bacterial pathogenesis against nematodes</title><author>Niu, Qiuhong ; 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Because of its genetic background and practical advantages over vertebrate model systems, the nematode Caenorhabditis elegans model has become an attractive host for studying microbial pathogenesis. Here we report a "Trojan horse" mechanism of bacterial pathogenesis against nematodes. We show that the bacterium Bacillus nematocida B16 lures nematodes by emitting potent volatile organic compounds that are much more attractive to worms than those from ordinary dietary bacteria. Seventeen B. nematocida-attractant volatile organic compounds are identified, and seven are individually confirmed to lure nematodes. Once the bacteria enter the intestine of nematodes, they secrete two proteases with broad substrate ranges but preferentially target essential intestinal proteins, leading to nematode death. This Trojan horse pattern of bacterium-nematode interaction enriches our understanding of microbial pathogenesis.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>20733068</pmid><doi>10.1073/pnas.1007276107</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bacillus Bacillus - pathogenicity Bacillus - physiology Bacteria Biological Sciences Caenorhabditis elegans Caenorhabditis elegans - microbiology Caenorhabditis elegans - physiology Genetics Host-pathogen interactions Host-Pathogen Interactions - physiology Intestine Intestines Intestines - microbiology Mortality Nematoda Nematodes Odorants Pathogenesis Pathogens Peptide Hydrolases - metabolism Proteases Proteinase Proteins Soil - parasitology Soil nematodes Virulence Virulence - physiology Virulence Factors - metabolism VOCs Volatile organic compounds Volatile Organic Compounds - metabolism Worms |
| title | Trojan horse mechanism of bacterial pathogenesis against nematodes |
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