Evidence for non genomic action of 17β estradiol on transepithelial resistance of human fetal membranes
Background : The role of hormones in the transport mechanisms of human fetal membranes in pregnancy is unclear. Estrogens are essential hormones in pregnancy and they play an important role in the ion transport via membranes. Aim : The aim of this study was to investigate the effect of 17β-estradiol...
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| Veröffentlicht in: | Journal of endocrinological investigation 2011-07, Vol.34 (7), p.510-514 |
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| container_title | Journal of endocrinological investigation |
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| creator | Verikouki, C. Hatzoglou, C. Zavos, A. I. Gourgoulianis, K. I. Molyvdas, P. A. Kallitsaris, A. Messinis, I. E. |
| description | Background
: The role of hormones in the transport mechanisms of human fetal membranes in pregnancy is unclear. Estrogens are essential hormones in pregnancy and they play an important role in the ion transport via membranes.
Aim
: The aim of this study was to investigate the effect of 17β-estradiol on transepithelial electrical resistance in the human amniochorion.
Material and methods
: Specimens of human fetal membranes were obtained. 17β-estradiol, tamoxifen and their combination were added in an Ussing chamber. Transepithelial resistance was measured before and after the addition of each solution.
Results
: An increase in transepithelial resistance was seen after the addition of estradiol to both sides of the membranes. The effect was rapid with a peak at the 1st min of application and dose-depended. Tamoxifen, caused a similar effect but smaller in magnitude and shorter in duration. Tamoxifen in combination with estradiol inhibited only in part the action of estradiol.
Conclusions
: These results suggest that estradiol induces a rapid increase of transepithelial resistance in human fetal membranes in vitro via a non-genomic pathway. It is possible those changes in transepithelial resistance play a role in the control of permeability of human amniochorion. |
| doi_str_mv | 10.3275/7206 |
| format | Article |
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: The role of hormones in the transport mechanisms of human fetal membranes in pregnancy is unclear. Estrogens are essential hormones in pregnancy and they play an important role in the ion transport via membranes.
Aim
: The aim of this study was to investigate the effect of 17β-estradiol on transepithelial electrical resistance in the human amniochorion.
Material and methods
: Specimens of human fetal membranes were obtained. 17β-estradiol, tamoxifen and their combination were added in an Ussing chamber. Transepithelial resistance was measured before and after the addition of each solution.
Results
: An increase in transepithelial resistance was seen after the addition of estradiol to both sides of the membranes. The effect was rapid with a peak at the 1st min of application and dose-depended. Tamoxifen, caused a similar effect but smaller in magnitude and shorter in duration. Tamoxifen in combination with estradiol inhibited only in part the action of estradiol.
Conclusions
: These results suggest that estradiol induces a rapid increase of transepithelial resistance in human fetal membranes in vitro via a non-genomic pathway. It is possible those changes in transepithelial resistance play a role in the control of permeability of human amniochorion.</description><identifier>ISSN: 0391-4097</identifier><identifier>EISSN: 1720-8386</identifier><identifier>DOI: 10.3275/7206</identifier><identifier>PMID: 20651472</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Dose-Response Relationship, Drug ; Electric Impedance ; Endocrinology ; Estradiol - pharmacology ; Estrogen Antagonists - pharmacology ; Extraembryonic Membranes - drug effects ; Extraembryonic Membranes - physiology ; Female ; Genome - drug effects ; Humans ; Medicine ; Medicine & Public Health ; Metabolic Diseases ; Original Articles ; Pregnancy ; Tamoxifen - pharmacology</subject><ispartof>Journal of endocrinological investigation, 2011-07, Vol.34 (7), p.510-514</ispartof><rights>Italian Society of Endocrinology (SIE) 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p240t-b8be1b57c147268d353967026fa2f64fc5f03950b9af74eb449198a663b506e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3275/7206$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3275/7206$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27933,27934,41497,42566,51328</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20651472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verikouki, C.</creatorcontrib><creatorcontrib>Hatzoglou, C.</creatorcontrib><creatorcontrib>Zavos, A. I.</creatorcontrib><creatorcontrib>Gourgoulianis, K. I.</creatorcontrib><creatorcontrib>Molyvdas, P. A.</creatorcontrib><creatorcontrib>Kallitsaris, A.</creatorcontrib><creatorcontrib>Messinis, I. E.</creatorcontrib><title>Evidence for non genomic action of 17β estradiol on transepithelial resistance of human fetal membranes</title><title>Journal of endocrinological investigation</title><addtitle>J Endocrinol Invest</addtitle><addtitle>J Endocrinol Invest</addtitle><description>Background
: The role of hormones in the transport mechanisms of human fetal membranes in pregnancy is unclear. Estrogens are essential hormones in pregnancy and they play an important role in the ion transport via membranes.
Aim
: The aim of this study was to investigate the effect of 17β-estradiol on transepithelial electrical resistance in the human amniochorion.
Material and methods
: Specimens of human fetal membranes were obtained. 17β-estradiol, tamoxifen and their combination were added in an Ussing chamber. Transepithelial resistance was measured before and after the addition of each solution.
Results
: An increase in transepithelial resistance was seen after the addition of estradiol to both sides of the membranes. The effect was rapid with a peak at the 1st min of application and dose-depended. Tamoxifen, caused a similar effect but smaller in magnitude and shorter in duration. Tamoxifen in combination with estradiol inhibited only in part the action of estradiol.
Conclusions
: These results suggest that estradiol induces a rapid increase of transepithelial resistance in human fetal membranes in vitro via a non-genomic pathway. It is possible those changes in transepithelial resistance play a role in the control of permeability of human amniochorion.</description><subject>Dose-Response Relationship, Drug</subject><subject>Electric Impedance</subject><subject>Endocrinology</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Antagonists - pharmacology</subject><subject>Extraembryonic Membranes - drug effects</subject><subject>Extraembryonic Membranes - physiology</subject><subject>Female</subject><subject>Genome - drug effects</subject><subject>Humans</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Metabolic Diseases</subject><subject>Original Articles</subject><subject>Pregnancy</subject><subject>Tamoxifen - pharmacology</subject><issn>0391-4097</issn><issn>1720-8386</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1OwzAQhS0EoqXlCsgblgE7_kuWqCoFqRIbWEd2Mm5dJU5kJ0hci4NwJhwVVm9-Po3ePITWlDywXIlHlRN5gZY0aVawQl6iJWElzTgp1QLdxHgihClWqGu0SKigXOVLdNx-ugZ8Ddj2Afve4wP4vnM11vXoUttbTNXPN4Y4Bt24vsVpmEofYXDjEVqnWxwgujjq-Uzij1OnPbYwpk0HnUkwxDW6srqNcPunK_TxvH3fvGT7t93r5mmfDTknY2YKA9QIVc_2ZNEwwUqpSC6tzq3kthY2fSWIKbVVHAznJS0LLSUzgkhQbIXuzneHyXTQVENwnQ5f1f_LCbg_AzGt_AFCdeqn4JOnipJqjrKao2S_JV5jlg</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Verikouki, C.</creator><creator>Hatzoglou, C.</creator><creator>Zavos, A. I.</creator><creator>Gourgoulianis, K. I.</creator><creator>Molyvdas, P. A.</creator><creator>Kallitsaris, A.</creator><creator>Messinis, I. E.</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20110701</creationdate><title>Evidence for non genomic action of 17β estradiol on transepithelial resistance of human fetal membranes</title><author>Verikouki, C. ; Hatzoglou, C. ; Zavos, A. I. ; Gourgoulianis, K. I. ; Molyvdas, P. A. ; Kallitsaris, A. ; Messinis, I. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p240t-b8be1b57c147268d353967026fa2f64fc5f03950b9af74eb449198a663b506e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Dose-Response Relationship, Drug</topic><topic>Electric Impedance</topic><topic>Endocrinology</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Antagonists - pharmacology</topic><topic>Extraembryonic Membranes - drug effects</topic><topic>Extraembryonic Membranes - physiology</topic><topic>Female</topic><topic>Genome - drug effects</topic><topic>Humans</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Metabolic Diseases</topic><topic>Original Articles</topic><topic>Pregnancy</topic><topic>Tamoxifen - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verikouki, C.</creatorcontrib><creatorcontrib>Hatzoglou, C.</creatorcontrib><creatorcontrib>Zavos, A. I.</creatorcontrib><creatorcontrib>Gourgoulianis, K. I.</creatorcontrib><creatorcontrib>Molyvdas, P. A.</creatorcontrib><creatorcontrib>Kallitsaris, A.</creatorcontrib><creatorcontrib>Messinis, I. E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of endocrinological investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verikouki, C.</au><au>Hatzoglou, C.</au><au>Zavos, A. I.</au><au>Gourgoulianis, K. I.</au><au>Molyvdas, P. A.</au><au>Kallitsaris, A.</au><au>Messinis, I. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for non genomic action of 17β estradiol on transepithelial resistance of human fetal membranes</atitle><jtitle>Journal of endocrinological investigation</jtitle><stitle>J Endocrinol Invest</stitle><addtitle>J Endocrinol Invest</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>34</volume><issue>7</issue><spage>510</spage><epage>514</epage><pages>510-514</pages><issn>0391-4097</issn><eissn>1720-8386</eissn><abstract>Background
: The role of hormones in the transport mechanisms of human fetal membranes in pregnancy is unclear. Estrogens are essential hormones in pregnancy and they play an important role in the ion transport via membranes.
Aim
: The aim of this study was to investigate the effect of 17β-estradiol on transepithelial electrical resistance in the human amniochorion.
Material and methods
: Specimens of human fetal membranes were obtained. 17β-estradiol, tamoxifen and their combination were added in an Ussing chamber. Transepithelial resistance was measured before and after the addition of each solution.
Results
: An increase in transepithelial resistance was seen after the addition of estradiol to both sides of the membranes. The effect was rapid with a peak at the 1st min of application and dose-depended. Tamoxifen, caused a similar effect but smaller in magnitude and shorter in duration. Tamoxifen in combination with estradiol inhibited only in part the action of estradiol.
Conclusions
: These results suggest that estradiol induces a rapid increase of transepithelial resistance in human fetal membranes in vitro via a non-genomic pathway. It is possible those changes in transepithelial resistance play a role in the control of permeability of human amniochorion.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>20651472</pmid><doi>10.3275/7206</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Dose-Response Relationship, Drug Electric Impedance Endocrinology Estradiol - pharmacology Estrogen Antagonists - pharmacology Extraembryonic Membranes - drug effects Extraembryonic Membranes - physiology Female Genome - drug effects Humans Medicine Medicine & Public Health Metabolic Diseases Original Articles Pregnancy Tamoxifen - pharmacology |
| title | Evidence for non genomic action of 17β estradiol on transepithelial resistance of human fetal membranes |
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