DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae
The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced. Herein we describe the cloning and sequencing of DPB2, the gene for the second largest subunit of DNA polymerase II, and the...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1991-06, Vol.88 (11), p.4601-4605 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Araki, H. (Osaka University, Suita, Osaka, Japan) Hamatake, R.K Johnston, L.H Sugino, A |
| description | The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced. Herein we describe the cloning and sequencing of DPB2, the gene for the second largest subunit of DNA polymerase II, and the isolation of temperature-sensitive dpb2 mutations. The DNA sequence revealed an open reading frame encoding a protein of Mr 79,461 and lacking significant sequence similarity to any protein in data bases. Disruption of DPB2 was lethal for the cell and the temperature-sensitive dpb2-1 mutant was partially defective in DNA synthesis at the restrictive temperature, indicating that the DPB2 protein is required for normal yeast chromosomal replication. Furthermore, the DNA polymerase II complex was difficult to obtain from dpb2-1 mutant cells, suggesting that a stable DNA polymerase II complex requires DPB2 and is essential for chromosomal replication. The DPB2 transcript periodically fluctuated during the cell cycle and, like those of other genes encoding DNA replication proteins, peaked at the G1/S phase boundary |
| doi_str_mv | 10.1073/pnas.88.11.4601 |
| format | Article |
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(Osaka University, Suita, Osaka, Japan) ; Hamatake, R.K ; Johnston, L.H ; Sugino, A</creator><creatorcontrib>Araki, H. (Osaka University, Suita, Osaka, Japan) ; Hamatake, R.K ; Johnston, L.H ; Sugino, A</creatorcontrib><description>The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced. Herein we describe the cloning and sequencing of DPB2, the gene for the second largest subunit of DNA polymerase II, and the isolation of temperature-sensitive dpb2 mutations. The DNA sequence revealed an open reading frame encoding a protein of Mr 79,461 and lacking significant sequence similarity to any protein in data bases. Disruption of DPB2 was lethal for the cell and the temperature-sensitive dpb2-1 mutant was partially defective in DNA synthesis at the restrictive temperature, indicating that the DPB2 protein is required for normal yeast chromosomal replication. Furthermore, the DNA polymerase II complex was difficult to obtain from dpb2-1 mutant cells, suggesting that a stable DNA polymerase II complex requires DPB2 and is essential for chromosomal replication. The DPB2 transcript periodically fluctuated during the cell cycle and, like those of other genes encoding DNA replication proteins, peaked at the G1/S phase boundary</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.88.11.4601</identifier><identifier>PMID: 2052544</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biochemistry ; Biological and medical sciences ; Cell Cycle ; Cell growth ; Chromosomes ; Chromosomes, Fungal ; CLONACION ; CLONAGE ; Cloning, Molecular ; CODE GENETIQUE ; CODIGO GENETICO ; DNA ; DNA Polymerase II - genetics ; DNA Polymerase II - isolation & purification ; DNA Replication ; DNA-directed DNA polymerase ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Fungal ; Genes. Genome ; Genetic mutation ; Genomic Library ; Hot Temperature ; Macromolecular Substances ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; MUTANT ; MUTANTES ; Mutation ; NUCLEOTIDE ; Nucleotide sequences ; NUCLEOTIDOS ; PEPTIDE ; PEPTIDOS ; Plasmids ; Restriction Mapping ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - growth & development ; Transcription, Genetic ; TRANSFERASAS ; TRANSFERASE ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1991-06, Vol.88 (11), p.4601-4605</ispartof><rights>Copyright 1991 The National Academy of Sciences of the United States of America</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-c93ed19b789df7a6e9e7a5ef5a1a923096a49c5367c9f4ab1ec882f8fae91e6f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/88/11.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2357100$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2357100$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19775427$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2052544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Araki, H. (Osaka University, Suita, Osaka, Japan)</creatorcontrib><creatorcontrib>Hamatake, R.K</creatorcontrib><creatorcontrib>Johnston, L.H</creatorcontrib><creatorcontrib>Sugino, A</creatorcontrib><title>DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced. Herein we describe the cloning and sequencing of DPB2, the gene for the second largest subunit of DNA polymerase II, and the isolation of temperature-sensitive dpb2 mutations. The DNA sequence revealed an open reading frame encoding a protein of Mr 79,461 and lacking significant sequence similarity to any protein in data bases. Disruption of DPB2 was lethal for the cell and the temperature-sensitive dpb2-1 mutant was partially defective in DNA synthesis at the restrictive temperature, indicating that the DPB2 protein is required for normal yeast chromosomal replication. Furthermore, the DNA polymerase II complex was difficult to obtain from dpb2-1 mutant cells, suggesting that a stable DNA polymerase II complex requires DPB2 and is essential for chromosomal replication. The DPB2 transcript periodically fluctuated during the cell cycle and, like those of other genes encoding DNA replication proteins, peaked at the G1/S phase boundary</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle</subject><subject>Cell growth</subject><subject>Chromosomes</subject><subject>Chromosomes, Fungal</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>Cloning, Molecular</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>DNA</subject><subject>DNA Polymerase II - genetics</subject><subject>DNA Polymerase II - isolation & purification</subject><subject>DNA Replication</subject><subject>DNA-directed DNA polymerase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Fungal</subject><subject>Genes. Genome</subject><subject>Genetic mutation</subject><subject>Genomic Library</subject><subject>Hot Temperature</subject><subject>Macromolecular Substances</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>Mutation</subject><subject>NUCLEOTIDE</subject><subject>Nucleotide sequences</subject><subject>NUCLEOTIDOS</subject><subject>PEPTIDE</subject><subject>PEPTIDOS</subject><subject>Plasmids</subject><subject>Restriction Mapping</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><subject>Transcription, Genetic</subject><subject>TRANSFERASAS</subject><subject>TRANSFERASE</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEURkeIqoTCGglB5Q1l06R-zIxtiU0fPCJVgFS6tm6c68TVjJ3aM4X8eyZKaOkGVpZ8zn3pK4pXjE4YleJkFSBPlJowNilryp4UI0Y1G9elpk-LEaVcjlXJy2fF85xvKKW6UnS_2Oe04lVZjoqfF9_P-DHplkgWGJBgsHHuw4JcfD0lq9isW0yQkUynJPezPviOnB0Tn0nC294nnBMXE7HLFNuYY4vD_6rxFjofA_GBXIG1Sxjo2mImFhPe-ewBXxR7DpqML3fvQXH96eOP8y_jy2-fp-enl2NbU9WNrRY4Z3omlZ47CTVqlFChq4CB5oLqGkptK1FLq10JM4ZWKe6UA9QMaycOig_bvqt-1uLcYugSNGaVfAtpbSJ485gEvzSLeGcqJpkYyo925Sne9pg70_pssWkgYOyzUbQWnOr_i6xSWmjKB_FkK9oUc07o7ndh1GwiNZtIjVKGMbOJdKh4-_cJ9_4uw4G_23HIFhqXIFifH9pqKauSy8E73HmbAX_wo0Hv_ykY1zdNh7-6wXyzNW9yF9PDRqKSjNIBv95iB9HAIg3bXF9pJhiXSvwGhZXVqQ</recordid><startdate>19910601</startdate><enddate>19910601</enddate><creator>Araki, H. (Osaka University, Suita, Osaka, Japan)</creator><creator>Hamatake, R.K</creator><creator>Johnston, L.H</creator><creator>Sugino, A</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>M81</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910601</creationdate><title>DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae</title><author>Araki, H. (Osaka University, Suita, Osaka, Japan) ; Hamatake, R.K ; Johnston, L.H ; Sugino, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c608t-c93ed19b789df7a6e9e7a5ef5a1a923096a49c5367c9f4ab1ec882f8fae91e6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle</topic><topic>Cell growth</topic><topic>Chromosomes</topic><topic>Chromosomes, Fungal</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>Cloning, Molecular</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>DNA</topic><topic>DNA Polymerase II - genetics</topic><topic>DNA Polymerase II - isolation & purification</topic><topic>DNA Replication</topic><topic>DNA-directed DNA polymerase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Fungal</topic><topic>Genes. 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(Osaka University, Suita, Osaka, Japan)</au><au>Hamatake, R.K</au><au>Johnston, L.H</au><au>Sugino, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1991-06-01</date><risdate>1991</risdate><volume>88</volume><issue>11</issue><spage>4601</spage><epage>4605</epage><pages>4601-4605</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The Saccharomyces cerevisiae DNA polymerase II holoenzyme consists of five polypeptides. The largest is the catalytic subunit, whose gene (POL2) has been cloned and sequenced. Herein we describe the cloning and sequencing of DPB2, the gene for the second largest subunit of DNA polymerase II, and the isolation of temperature-sensitive dpb2 mutations. The DNA sequence revealed an open reading frame encoding a protein of Mr 79,461 and lacking significant sequence similarity to any protein in data bases. Disruption of DPB2 was lethal for the cell and the temperature-sensitive dpb2-1 mutant was partially defective in DNA synthesis at the restrictive temperature, indicating that the DPB2 protein is required for normal yeast chromosomal replication. Furthermore, the DNA polymerase II complex was difficult to obtain from dpb2-1 mutant cells, suggesting that a stable DNA polymerase II complex requires DPB2 and is essential for chromosomal replication. The DPB2 transcript periodically fluctuated during the cell cycle and, like those of other genes encoding DNA replication proteins, peaked at the G1/S phase boundary</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2052544</pmid><doi>10.1073/pnas.88.11.4601</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Base Sequence Biochemistry Biological and medical sciences Cell Cycle Cell growth Chromosomes Chromosomes, Fungal CLONACION CLONAGE Cloning, Molecular CODE GENETIQUE CODIGO GENETICO DNA DNA Polymerase II - genetics DNA Polymerase II - isolation & purification DNA Replication DNA-directed DNA polymerase Fundamental and applied biological sciences. Psychology Genes Genes, Fungal Genes. Genome Genetic mutation Genomic Library Hot Temperature Macromolecular Substances Molecular and cellular biology Molecular genetics Molecular Sequence Data MUTANT MUTANTES Mutation NUCLEOTIDE Nucleotide sequences NUCLEOTIDOS PEPTIDE PEPTIDOS Plasmids Restriction Mapping SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development Transcription, Genetic TRANSFERASAS TRANSFERASE Yeasts |
| title | DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae |
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