Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc)
Polychlorinated biphenyls (PCBs) are environmental pollutants that are quite toxic to biological systems. This study examined the inhibitory effect of PCB126 and PCB114 on testicular steroidogenesis in male rats. Male Sprague Dawley rats received weekly intraperitoneal injections of PCB126 (0.2 mg/k...
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| Veröffentlicht in: | Toxicology and industrial health 2010-06, Vol.26 (5), p.287 |
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| creator | Han, Dae-Yong Kang, Sang-Rim Park, Oh-Sung Cho, Jae-Hyeon Won, Chung-Kil Park, Hyeon-Soo Park, Kwang-Il Kim, Eun-Hee Kim, Gon-Sup |
| description | Polychlorinated biphenyls (PCBs) are environmental pollutants that are quite toxic to biological systems. This study examined the inhibitory effect of PCB126 and PCB114 on testicular steroidogenesis in male rats. Male Sprague Dawley rats received weekly intraperitoneal injections of PCB126 (0.2 mg/kg) or PCB114 (20 mg/kg) or vehicle (corn oil). Animals from each group were sacrificed at 2, 5 and 8 weeks after the injections. Blood and testis tissue samples were collected for the hormone assay, Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). The testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were assayed, and the expression levels of the mRNA and proteins associated with the testosterone biosynthesis pathway were measured to determine the effect of PCB126 and PCB114 on testicular steroidogenesis. The results showed that the testis weight was significantly higher in the PCB126-treated rats given eight shots. Moreover, the serum testosterone levels were significantly lower in the PCB126 and PCB114-treated groups than the control. The transcription and translation levels of P450(17alpha) and P450(scc) were significantly lower in the PCB126-treated groups than the control. These results suggest that PCB126 may affect testicular steroidogenesis by downregulating P450(17alpha), P450(scc) and have inhibitory effect on the testicular functions. |
| doi_str_mv | 10.1177/0748233710364961 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_20356859</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20356859</sourcerecordid><originalsourceid>FETCH-LOGICAL-p140t-eef01759321f73e9387470f21078f93792c013548b957e9cda78533e079f82353</originalsourceid><addsrcrecordid>eNo1kD1PwzAURS0kREthZ0IeYQg8x3FePKKKL6kSHWCunOS5MUrjyHZBmfjrVCpMV7r36A6HsSsBd0Ig3gMWVS4lCpBloUtxwuaiQMxAajlj5zF-AkBZqvyMzXKQqqyUnrOfte-nput9cINJ1PLajR0NUx95Z76Iu6FztUs-TJyspSZxP_BEMblm35vAY6LgXeu3NFB0kdcTb_33EGh7mJM7wN7ydaHgRqDpx87ccjO0xyY2ze0FO7Wmj3T5lwv28fT4vnzJVm_Pr8uHVTaKAlJGZEGg0jIXFiVpWWGBYHMBWFktUecNCKmKqtYKSTetwUpJSYDaHqwouWDXx99xX--o3YzB7UyYNv8m5C8LkV4g</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc)</title><source>Access via SAGE</source><source>MEDLINE</source><creator>Han, Dae-Yong ; Kang, Sang-Rim ; Park, Oh-Sung ; Cho, Jae-Hyeon ; Won, Chung-Kil ; Park, Hyeon-Soo ; Park, Kwang-Il ; Kim, Eun-Hee ; Kim, Gon-Sup</creator><creatorcontrib>Han, Dae-Yong ; Kang, Sang-Rim ; Park, Oh-Sung ; Cho, Jae-Hyeon ; Won, Chung-Kil ; Park, Hyeon-Soo ; Park, Kwang-Il ; Kim, Eun-Hee ; Kim, Gon-Sup</creatorcontrib><description>Polychlorinated biphenyls (PCBs) are environmental pollutants that are quite toxic to biological systems. This study examined the inhibitory effect of PCB126 and PCB114 on testicular steroidogenesis in male rats. Male Sprague Dawley rats received weekly intraperitoneal injections of PCB126 (0.2 mg/kg) or PCB114 (20 mg/kg) or vehicle (corn oil). Animals from each group were sacrificed at 2, 5 and 8 weeks after the injections. Blood and testis tissue samples were collected for the hormone assay, Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). The testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were assayed, and the expression levels of the mRNA and proteins associated with the testosterone biosynthesis pathway were measured to determine the effect of PCB126 and PCB114 on testicular steroidogenesis. The results showed that the testis weight was significantly higher in the PCB126-treated rats given eight shots. Moreover, the serum testosterone levels were significantly lower in the PCB126 and PCB114-treated groups than the control. The transcription and translation levels of P450(17alpha) and P450(scc) were significantly lower in the PCB126-treated groups than the control. These results suggest that PCB126 may affect testicular steroidogenesis by downregulating P450(17alpha), P450(scc) and have inhibitory effect on the testicular functions.</description><identifier>EISSN: 1477-0393</identifier><identifier>DOI: 10.1177/0748233710364961</identifier><identifier>PMID: 20356859</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Blotting, Western ; Cholesterol Side-Chain Cleavage Enzyme - biosynthesis ; Cholesterol Side-Chain Cleavage Enzyme - genetics ; Cholesterol Side-Chain Cleavage Enzyme - metabolism ; Down-Regulation - drug effects ; Follicle Stimulating Hormone - biosynthesis ; Follicle Stimulating Hormone - blood ; Gene Expression Profiling ; Humans ; Luteinizing Hormone - biosynthesis ; Luteinizing Hormone - blood ; Male ; Organ Size - drug effects ; Polychlorinated Biphenyls - pharmacology ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Steroid 17-alpha-Hydroxylase - biosynthesis ; Steroid 17-alpha-Hydroxylase - genetics ; Steroid 17-alpha-Hydroxylase - metabolism ; Steroids - antagonists & inhibitors ; Steroids - biosynthesis ; Testis - anatomy & histology ; Testis - drug effects ; Testis - metabolism ; Testosterone - biosynthesis ; Testosterone - blood</subject><ispartof>Toxicology and industrial health, 2010-06, Vol.26 (5), p.287</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20356859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Dae-Yong</creatorcontrib><creatorcontrib>Kang, Sang-Rim</creatorcontrib><creatorcontrib>Park, Oh-Sung</creatorcontrib><creatorcontrib>Cho, Jae-Hyeon</creatorcontrib><creatorcontrib>Won, Chung-Kil</creatorcontrib><creatorcontrib>Park, Hyeon-Soo</creatorcontrib><creatorcontrib>Park, Kwang-Il</creatorcontrib><creatorcontrib>Kim, Eun-Hee</creatorcontrib><creatorcontrib>Kim, Gon-Sup</creatorcontrib><title>Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc)</title><title>Toxicology and industrial health</title><addtitle>Toxicol Ind Health</addtitle><description>Polychlorinated biphenyls (PCBs) are environmental pollutants that are quite toxic to biological systems. This study examined the inhibitory effect of PCB126 and PCB114 on testicular steroidogenesis in male rats. Male Sprague Dawley rats received weekly intraperitoneal injections of PCB126 (0.2 mg/kg) or PCB114 (20 mg/kg) or vehicle (corn oil). Animals from each group were sacrificed at 2, 5 and 8 weeks after the injections. Blood and testis tissue samples were collected for the hormone assay, Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). The testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were assayed, and the expression levels of the mRNA and proteins associated with the testosterone biosynthesis pathway were measured to determine the effect of PCB126 and PCB114 on testicular steroidogenesis. The results showed that the testis weight was significantly higher in the PCB126-treated rats given eight shots. Moreover, the serum testosterone levels were significantly lower in the PCB126 and PCB114-treated groups than the control. The transcription and translation levels of P450(17alpha) and P450(scc) were significantly lower in the PCB126-treated groups than the control. These results suggest that PCB126 may affect testicular steroidogenesis by downregulating P450(17alpha), P450(scc) and have inhibitory effect on the testicular functions.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cholesterol Side-Chain Cleavage Enzyme - biosynthesis</subject><subject>Cholesterol Side-Chain Cleavage Enzyme - genetics</subject><subject>Cholesterol Side-Chain Cleavage Enzyme - metabolism</subject><subject>Down-Regulation - drug effects</subject><subject>Follicle Stimulating Hormone - biosynthesis</subject><subject>Follicle Stimulating Hormone - blood</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>Luteinizing Hormone - biosynthesis</subject><subject>Luteinizing Hormone - blood</subject><subject>Male</subject><subject>Organ Size - drug effects</subject><subject>Polychlorinated Biphenyls - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Steroid 17-alpha-Hydroxylase - biosynthesis</subject><subject>Steroid 17-alpha-Hydroxylase - genetics</subject><subject>Steroid 17-alpha-Hydroxylase - metabolism</subject><subject>Steroids - antagonists & inhibitors</subject><subject>Steroids - biosynthesis</subject><subject>Testis - anatomy & histology</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testosterone - biosynthesis</subject><subject>Testosterone - blood</subject><issn>1477-0393</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kD1PwzAURS0kREthZ0IeYQg8x3FePKKKL6kSHWCunOS5MUrjyHZBmfjrVCpMV7r36A6HsSsBd0Ig3gMWVS4lCpBloUtxwuaiQMxAajlj5zF-AkBZqvyMzXKQqqyUnrOfte-nput9cINJ1PLajR0NUx95Z76Iu6FztUs-TJyspSZxP_BEMblm35vAY6LgXeu3NFB0kdcTb_33EGh7mJM7wN7ydaHgRqDpx87ccjO0xyY2ze0FO7Wmj3T5lwv28fT4vnzJVm_Pr8uHVTaKAlJGZEGg0jIXFiVpWWGBYHMBWFktUecNCKmKqtYKSTetwUpJSYDaHqwouWDXx99xX--o3YzB7UyYNv8m5C8LkV4g</recordid><startdate>201006</startdate><enddate>201006</enddate><creator>Han, Dae-Yong</creator><creator>Kang, Sang-Rim</creator><creator>Park, Oh-Sung</creator><creator>Cho, Jae-Hyeon</creator><creator>Won, Chung-Kil</creator><creator>Park, Hyeon-Soo</creator><creator>Park, Kwang-Il</creator><creator>Kim, Eun-Hee</creator><creator>Kim, Gon-Sup</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>201006</creationdate><title>Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc)</title><author>Han, Dae-Yong ; Kang, Sang-Rim ; Park, Oh-Sung ; Cho, Jae-Hyeon ; Won, Chung-Kil ; Park, Hyeon-Soo ; Park, Kwang-Il ; Kim, Eun-Hee ; Kim, Gon-Sup</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p140t-eef01759321f73e9387470f21078f93792c013548b957e9cda78533e079f82353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cholesterol Side-Chain Cleavage Enzyme - biosynthesis</topic><topic>Cholesterol Side-Chain Cleavage Enzyme - genetics</topic><topic>Cholesterol Side-Chain Cleavage Enzyme - metabolism</topic><topic>Down-Regulation - drug effects</topic><topic>Follicle Stimulating Hormone - biosynthesis</topic><topic>Follicle Stimulating Hormone - blood</topic><topic>Gene Expression Profiling</topic><topic>Humans</topic><topic>Luteinizing Hormone - biosynthesis</topic><topic>Luteinizing Hormone - blood</topic><topic>Male</topic><topic>Organ Size - drug effects</topic><topic>Polychlorinated Biphenyls - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Steroid 17-alpha-Hydroxylase - biosynthesis</topic><topic>Steroid 17-alpha-Hydroxylase - genetics</topic><topic>Steroid 17-alpha-Hydroxylase - metabolism</topic><topic>Steroids - antagonists & inhibitors</topic><topic>Steroids - biosynthesis</topic><topic>Testis - anatomy & histology</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testosterone - biosynthesis</topic><topic>Testosterone - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Dae-Yong</creatorcontrib><creatorcontrib>Kang, Sang-Rim</creatorcontrib><creatorcontrib>Park, Oh-Sung</creatorcontrib><creatorcontrib>Cho, Jae-Hyeon</creatorcontrib><creatorcontrib>Won, Chung-Kil</creatorcontrib><creatorcontrib>Park, Hyeon-Soo</creatorcontrib><creatorcontrib>Park, Kwang-Il</creatorcontrib><creatorcontrib>Kim, Eun-Hee</creatorcontrib><creatorcontrib>Kim, Gon-Sup</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Toxicology and industrial health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Dae-Yong</au><au>Kang, Sang-Rim</au><au>Park, Oh-Sung</au><au>Cho, Jae-Hyeon</au><au>Won, Chung-Kil</au><au>Park, Hyeon-Soo</au><au>Park, Kwang-Il</au><au>Kim, Eun-Hee</au><au>Kim, Gon-Sup</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc)</atitle><jtitle>Toxicology and industrial health</jtitle><addtitle>Toxicol Ind Health</addtitle><date>2010-06</date><risdate>2010</risdate><volume>26</volume><issue>5</issue><spage>287</spage><pages>287-</pages><eissn>1477-0393</eissn><abstract>Polychlorinated biphenyls (PCBs) are environmental pollutants that are quite toxic to biological systems. This study examined the inhibitory effect of PCB126 and PCB114 on testicular steroidogenesis in male rats. Male Sprague Dawley rats received weekly intraperitoneal injections of PCB126 (0.2 mg/kg) or PCB114 (20 mg/kg) or vehicle (corn oil). Animals from each group were sacrificed at 2, 5 and 8 weeks after the injections. Blood and testis tissue samples were collected for the hormone assay, Western blotting and reverse transcriptase polymerase chain reaction (RT-PCR). The testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels were assayed, and the expression levels of the mRNA and proteins associated with the testosterone biosynthesis pathway were measured to determine the effect of PCB126 and PCB114 on testicular steroidogenesis. The results showed that the testis weight was significantly higher in the PCB126-treated rats given eight shots. Moreover, the serum testosterone levels were significantly lower in the PCB126 and PCB114-treated groups than the control. The transcription and translation levels of P450(17alpha) and P450(scc) were significantly lower in the PCB126-treated groups than the control. These results suggest that PCB126 may affect testicular steroidogenesis by downregulating P450(17alpha), P450(scc) and have inhibitory effect on the testicular functions.</abstract><cop>England</cop><pmid>20356859</pmid><doi>10.1177/0748233710364961</doi></addata></record> |
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| subjects | Animals Blotting, Western Cholesterol Side-Chain Cleavage Enzyme - biosynthesis Cholesterol Side-Chain Cleavage Enzyme - genetics Cholesterol Side-Chain Cleavage Enzyme - metabolism Down-Regulation - drug effects Follicle Stimulating Hormone - biosynthesis Follicle Stimulating Hormone - blood Gene Expression Profiling Humans Luteinizing Hormone - biosynthesis Luteinizing Hormone - blood Male Organ Size - drug effects Polychlorinated Biphenyls - pharmacology Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Steroid 17-alpha-Hydroxylase - biosynthesis Steroid 17-alpha-Hydroxylase - genetics Steroid 17-alpha-Hydroxylase - metabolism Steroids - antagonists & inhibitors Steroids - biosynthesis Testis - anatomy & histology Testis - drug effects Testis - metabolism Testosterone - biosynthesis Testosterone - blood |
| title | Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc) |
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