Expression and Alteration of p16 in Diffuse Large B Cell Lymphoma
Objectives: This study aimed to examine (1) the expression of P16 protein relative to sites of presentation, immunophenotypic subgroups and proliferative indices of tumour cells, and (2) the relationship between p16 gene alterations and P16 protein overexpression in 70 cases of diffuse large B cell...
Gespeichert in:
| Veröffentlicht in: | Pathobiology (Basel) 2010-01, Vol.77 (2), p.96-105 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 105 |
|---|---|
| container_issue | 2 |
| container_start_page | 96 |
| container_title | Pathobiology (Basel) |
| container_volume | 77 |
| creator | Lee, Ee-Soo Kim, Lian-Hua Abdullah, Wan Ariffin Peh, Suat-Cheng |
| description | Objectives: This study aimed to examine (1) the expression of P16 protein relative to sites of presentation, immunophenotypic subgroups and proliferative indices of tumour cells, and (2) the relationship between p16 gene alterations and P16 protein overexpression in 70 cases of diffuse large B cell lymphoma (DLBCL). Methods: Expression of P16, CD10, BCL-6, MUM-1 and proliferation marker (Ki-67) was demonstrated by immunohistochemistry. Fluorescence in situ hybridization (FISH) was employed to detect p16 alterations. Results: P16 overexpression was shown in 45.7% (32/70) of the DLBCL cases, and was significantly correlated with CD10 (p = 0.022) and germinal centre B-cell-like (GCB) phenotype (p = 0.022). High expression of P16 was inversely associated with high proliferative activity (Ki-67 index greater than 75%) (p = 0.020). Of the 47 cases that yielded interpretable FISH results, 57.4% (27/47) showed deletions of p16 and 27.7% (13/47) showed gains of p16. P16 overexpression and p16 deletions were mutually exclusive (p = 0.019). There was no correlation between P16 overexpression and p16 gains (p = 0.621). Conclusions: The GCB and non-GCB subgroups of DLBCLs show different patterns of P16 expression. High levels of P16 may mitigate tumour cell proliferation. Gains of p16 do not necessarily increase P16 protein expression. |
| doi_str_mv | 10.1159/000278291 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmed_primary_20332669</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2065308241</sourcerecordid><originalsourceid>FETCH-LOGICAL-c399t-a22d46ff200094abff3ae4405d41a43db8aaf2ccc8ab3b2a3a8c00e125ea85ba3</originalsourceid><addsrcrecordid>eNqF0DtPwzAQB3ALgWgpDOwIWSyIIeBnao-llIcUiQXm6JLYkNI8sBOJfntcpRSJhcln6Xenuz9Cp5RcUyr1DSGETRXTdA-NqWA8IqHeDzWhMmKEqBE68n4ZmCIxOUQjRjhncazHaLb4ap3xvmxqDHWBZ6vOOOg238bilsa4rPFdaW3vDU7AvRl8i-dmtcLJumrfmwqO0YGFlTcn23eCXu8XL_PHKHl-eJrPkijnWncRMFaI2NqwDtECMms5GCGILAQFwYtMAViW57mCjGcMOKicEEOZNKBkBnyCLoe5rWs-e-O7tCp9HjaB2jS9T6dCSKWk1v_LcLyUnPIgL_7IZdO7OpyRSjqVQis9DehqQLlrvHfGpq0rK3DrlJJ0k3-6yz_Y8-3APqtMsZM_gQdwNoCPTZbuFwz935wlhkk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>517549897</pqid></control><display><type>article</type><title>Expression and Alteration of p16 in Diffuse Large B Cell Lymphoma</title><source>MEDLINE</source><source>Karger Journals</source><source>Alma/SFX Local Collection</source><creator>Lee, Ee-Soo ; Kim, Lian-Hua ; Abdullah, Wan Ariffin ; Peh, Suat-Cheng</creator><creatorcontrib>Lee, Ee-Soo ; Kim, Lian-Hua ; Abdullah, Wan Ariffin ; Peh, Suat-Cheng</creatorcontrib><description>Objectives: This study aimed to examine (1) the expression of P16 protein relative to sites of presentation, immunophenotypic subgroups and proliferative indices of tumour cells, and (2) the relationship between p16 gene alterations and P16 protein overexpression in 70 cases of diffuse large B cell lymphoma (DLBCL). Methods: Expression of P16, CD10, BCL-6, MUM-1 and proliferation marker (Ki-67) was demonstrated by immunohistochemistry. Fluorescence in situ hybridization (FISH) was employed to detect p16 alterations. Results: P16 overexpression was shown in 45.7% (32/70) of the DLBCL cases, and was significantly correlated with CD10 (p = 0.022) and germinal centre B-cell-like (GCB) phenotype (p = 0.022). High expression of P16 was inversely associated with high proliferative activity (Ki-67 index greater than 75%) (p = 0.020). Of the 47 cases that yielded interpretable FISH results, 57.4% (27/47) showed deletions of p16 and 27.7% (13/47) showed gains of p16. P16 overexpression and p16 deletions were mutually exclusive (p = 0.019). There was no correlation between P16 overexpression and p16 gains (p = 0.621). Conclusions: The GCB and non-GCB subgroups of DLBCLs show different patterns of P16 expression. High levels of P16 may mitigate tumour cell proliferation. Gains of p16 do not necessarily increase P16 protein expression.</description><identifier>ISSN: 1015-2008</identifier><identifier>EISSN: 1423-0291</identifier><identifier>DOI: 10.1159/000278291</identifier><identifier>PMID: 20332669</identifier><identifier>CODEN: PATHEF</identifier><language>eng</language><publisher>Basel, Switzerland: S. Karger AG</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Cell Proliferation ; Cells ; Child ; Child, Preschool ; Cyclin-Dependent Kinase Inhibitor p16 - genetics ; Cyclin-Dependent Kinase Inhibitor p16 - metabolism ; Female ; Fluorescence in situ hybridization ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genes, p16 ; Genotype & phenotype ; Humans ; Immunoenzyme Techniques ; In Situ Hybridization, Fluorescence ; Lymphoma ; Lymphoma, Large B-Cell, Diffuse - genetics ; Lymphoma, Large B-Cell, Diffuse - metabolism ; Lymphoma, Large B-Cell, Diffuse - pathology ; Male ; Middle Aged ; Original Paper ; Proteins ; Tumors</subject><ispartof>Pathobiology (Basel), 2010-01, Vol.77 (2), p.96-105</ispartof><rights>2010 S. Karger AG, Basel</rights><rights>Copyright (c) 2010 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-a22d46ff200094abff3ae4405d41a43db8aaf2ccc8ab3b2a3a8c00e125ea85ba3</citedby><cites>FETCH-LOGICAL-c399t-a22d46ff200094abff3ae4405d41a43db8aaf2ccc8ab3b2a3a8c00e125ea85ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2429,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20332669$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Ee-Soo</creatorcontrib><creatorcontrib>Kim, Lian-Hua</creatorcontrib><creatorcontrib>Abdullah, Wan Ariffin</creatorcontrib><creatorcontrib>Peh, Suat-Cheng</creatorcontrib><title>Expression and Alteration of p16 in Diffuse Large B Cell Lymphoma</title><title>Pathobiology (Basel)</title><addtitle>Pathobiology</addtitle><description>Objectives: This study aimed to examine (1) the expression of P16 protein relative to sites of presentation, immunophenotypic subgroups and proliferative indices of tumour cells, and (2) the relationship between p16 gene alterations and P16 protein overexpression in 70 cases of diffuse large B cell lymphoma (DLBCL). Methods: Expression of P16, CD10, BCL-6, MUM-1 and proliferation marker (Ki-67) was demonstrated by immunohistochemistry. Fluorescence in situ hybridization (FISH) was employed to detect p16 alterations. Results: P16 overexpression was shown in 45.7% (32/70) of the DLBCL cases, and was significantly correlated with CD10 (p = 0.022) and germinal centre B-cell-like (GCB) phenotype (p = 0.022). High expression of P16 was inversely associated with high proliferative activity (Ki-67 index greater than 75%) (p = 0.020). Of the 47 cases that yielded interpretable FISH results, 57.4% (27/47) showed deletions of p16 and 27.7% (13/47) showed gains of p16. P16 overexpression and p16 deletions were mutually exclusive (p = 0.019). There was no correlation between P16 overexpression and p16 gains (p = 0.621). Conclusions: The GCB and non-GCB subgroups of DLBCLs show different patterns of P16 expression. High levels of P16 may mitigate tumour cell proliferation. Gains of p16 do not necessarily increase P16 protein expression.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Cell Proliferation</subject><subject>Cells</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - genetics</subject><subject>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</subject><subject>Female</subject><subject>Fluorescence in situ hybridization</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes, p16</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Lymphoma</subject><subject>Lymphoma, Large B-Cell, Diffuse - genetics</subject><subject>Lymphoma, Large B-Cell, Diffuse - metabolism</subject><subject>Lymphoma, Large B-Cell, Diffuse - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Original Paper</subject><subject>Proteins</subject><subject>Tumors</subject><issn>1015-2008</issn><issn>1423-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0DtPwzAQB3ALgWgpDOwIWSyIIeBnao-llIcUiQXm6JLYkNI8sBOJfntcpRSJhcln6Xenuz9Cp5RcUyr1DSGETRXTdA-NqWA8IqHeDzWhMmKEqBE68n4ZmCIxOUQjRjhncazHaLb4ap3xvmxqDHWBZ6vOOOg238bilsa4rPFdaW3vDU7AvRl8i-dmtcLJumrfmwqO0YGFlTcn23eCXu8XL_PHKHl-eJrPkijnWncRMFaI2NqwDtECMms5GCGILAQFwYtMAViW57mCjGcMOKicEEOZNKBkBnyCLoe5rWs-e-O7tCp9HjaB2jS9T6dCSKWk1v_LcLyUnPIgL_7IZdO7OpyRSjqVQis9DehqQLlrvHfGpq0rK3DrlJJ0k3-6yz_Y8-3APqtMsZM_gQdwNoCPTZbuFwz935wlhkk</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Lee, Ee-Soo</creator><creator>Kim, Lian-Hua</creator><creator>Abdullah, Wan Ariffin</creator><creator>Peh, Suat-Cheng</creator><general>S. Karger AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20100101</creationdate><title>Expression and Alteration of p16 in Diffuse Large B Cell Lymphoma</title><author>Lee, Ee-Soo ; Kim, Lian-Hua ; Abdullah, Wan Ariffin ; Peh, Suat-Cheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-a22d46ff200094abff3ae4405d41a43db8aaf2ccc8ab3b2a3a8c00e125ea85ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Cell Proliferation</topic><topic>Cells</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - genetics</topic><topic>Cyclin-Dependent Kinase Inhibitor p16 - metabolism</topic><topic>Female</topic><topic>Fluorescence in situ hybridization</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes, p16</topic><topic>Genotype & phenotype</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Lymphoma</topic><topic>Lymphoma, Large B-Cell, Diffuse - genetics</topic><topic>Lymphoma, Large B-Cell, Diffuse - metabolism</topic><topic>Lymphoma, Large B-Cell, Diffuse - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Original Paper</topic><topic>Proteins</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Ee-Soo</creatorcontrib><creatorcontrib>Kim, Lian-Hua</creatorcontrib><creatorcontrib>Abdullah, Wan Ariffin</creatorcontrib><creatorcontrib>Peh, Suat-Cheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Pathobiology (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Ee-Soo</au><au>Kim, Lian-Hua</au><au>Abdullah, Wan Ariffin</au><au>Peh, Suat-Cheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and Alteration of p16 in Diffuse Large B Cell Lymphoma</atitle><jtitle>Pathobiology (Basel)</jtitle><addtitle>Pathobiology</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>77</volume><issue>2</issue><spage>96</spage><epage>105</epage><pages>96-105</pages><issn>1015-2008</issn><eissn>1423-0291</eissn><coden>PATHEF</coden><abstract>Objectives: This study aimed to examine (1) the expression of P16 protein relative to sites of presentation, immunophenotypic subgroups and proliferative indices of tumour cells, and (2) the relationship between p16 gene alterations and P16 protein overexpression in 70 cases of diffuse large B cell lymphoma (DLBCL). Methods: Expression of P16, CD10, BCL-6, MUM-1 and proliferation marker (Ki-67) was demonstrated by immunohistochemistry. Fluorescence in situ hybridization (FISH) was employed to detect p16 alterations. Results: P16 overexpression was shown in 45.7% (32/70) of the DLBCL cases, and was significantly correlated with CD10 (p = 0.022) and germinal centre B-cell-like (GCB) phenotype (p = 0.022). High expression of P16 was inversely associated with high proliferative activity (Ki-67 index greater than 75%) (p = 0.020). Of the 47 cases that yielded interpretable FISH results, 57.4% (27/47) showed deletions of p16 and 27.7% (13/47) showed gains of p16. P16 overexpression and p16 deletions were mutually exclusive (p = 0.019). There was no correlation between P16 overexpression and p16 gains (p = 0.621). Conclusions: The GCB and non-GCB subgroups of DLBCLs show different patterns of P16 expression. High levels of P16 may mitigate tumour cell proliferation. Gains of p16 do not necessarily increase P16 protein expression.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>20332669</pmid><doi>10.1159/000278291</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1015-2008 |
| ispartof | Pathobiology (Basel), 2010-01, Vol.77 (2), p.96-105 |
| issn | 1015-2008 1423-0291 |
| language | eng |
| recordid | cdi_pubmed_primary_20332669 |
| source | MEDLINE; Karger Journals; Alma/SFX Local Collection |
| subjects | Adult Aged Aged, 80 and over Cell Proliferation Cells Child Child, Preschool Cyclin-Dependent Kinase Inhibitor p16 - genetics Cyclin-Dependent Kinase Inhibitor p16 - metabolism Female Fluorescence in situ hybridization Gene expression Gene Expression Regulation, Neoplastic Genes, p16 Genotype & phenotype Humans Immunoenzyme Techniques In Situ Hybridization, Fluorescence Lymphoma Lymphoma, Large B-Cell, Diffuse - genetics Lymphoma, Large B-Cell, Diffuse - metabolism Lymphoma, Large B-Cell, Diffuse - pathology Male Middle Aged Original Paper Proteins Tumors |
| title | Expression and Alteration of p16 in Diffuse Large B Cell Lymphoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T10%3A52%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20and%20Alteration%20of%20p16%20in%20Diffuse%20Large%20B%20Cell%20Lymphoma&rft.jtitle=Pathobiology%20(Basel)&rft.au=Lee,%20Ee-Soo&rft.date=2010-01-01&rft.volume=77&rft.issue=2&rft.spage=96&rft.epage=105&rft.pages=96-105&rft.issn=1015-2008&rft.eissn=1423-0291&rft.coden=PATHEF&rft_id=info:doi/10.1159/000278291&rft_dat=%3Cproquest_pubme%3E2065308241%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=517549897&rft_id=info:pmid/20332669&rfr_iscdi=true |