Comparative Evaluation of an Automated Repetitive-Sequence-Based PCR Instrument versus Pulsed-Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak
A semiautomated, repetitive-sequence-based PCR (rep-PCR) instrument (DiversiLab system) was evaluated in comparison with pulsed-field gel electrophoresis (PFGE) to investigate an outbreak of Serratia marcescens infections in a neonatal intensive care unit (NICU). A selection of 36 epidemiologically...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2010-05, Vol.48 (5), p.1690-1695 |
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| creator | Ligozzi, Marco Fontana, Roberta Aldegheri, Marco Scalet, Giovanna Lo Cascio, Giuliana |
| description | A semiautomated, repetitive-sequence-based PCR (rep-PCR) instrument (DiversiLab system) was evaluated in comparison with pulsed-field gel electrophoresis (PFGE) to investigate an outbreak of Serratia marcescens infections in a neonatal intensive care unit (NICU). A selection of 36 epidemiologically related and 8 epidemiologically unrelated isolates was analyzed. Among the epidemiologically related isolates, PFGE identified five genetically unrelated patterns. Thirty-two isolates from patients and wet nurses showed the same PFGE profile (pattern A). Genetically unrelated PFGE patterns were found in one patient (pattern B), in two wet nurses (patterns C and D), and in an environmental isolate from the NICU (pattern G). Rep-PCR identified seven different patterns, three of which included the 32 isolates of PFGE type A. One or two band differences in isolates of these three types allowed isolates to be categorized as similar and included in a unique cluster. Isolates of different PFGE types were also of unrelated rep-PCR types. All of the epidemiologically unrelated isolates were of different PFGE and rep-PCR types. The level of discrimination exhibited by rep-PCR with the DiversiLab system allowed us to conclude that this method was able to identify genetic similarity in a spatio-temporal cluster of S. marcescens isolates. |
| doi_str_mv | 10.1128/JCM.01528-09 |
| format | Article |
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A selection of 36 epidemiologically related and 8 epidemiologically unrelated isolates was analyzed. Among the epidemiologically related isolates, PFGE identified five genetically unrelated patterns. Thirty-two isolates from patients and wet nurses showed the same PFGE profile (pattern A). Genetically unrelated PFGE patterns were found in one patient (pattern B), in two wet nurses (patterns C and D), and in an environmental isolate from the NICU (pattern G). Rep-PCR identified seven different patterns, three of which included the 32 isolates of PFGE type A. One or two band differences in isolates of these three types allowed isolates to be categorized as similar and included in a unique cluster. Isolates of different PFGE types were also of unrelated rep-PCR types. All of the epidemiologically unrelated isolates were of different PFGE and rep-PCR types. The level of discrimination exhibited by rep-PCR with the DiversiLab system allowed us to conclude that this method was able to identify genetic similarity in a spatio-temporal cluster of S. marcescens isolates.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.01528-09</identifier><identifier>PMID: 20237095</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Adult ; Automation - methods ; Bacterial Typing Techniques - methods ; Bacteriology ; Biological and medical sciences ; Cluster Analysis ; Cross Infection - epidemiology ; Cross Infection - microbiology ; Disease Outbreaks ; DNA, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field - methods ; Environmental Microbiology ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Genotype ; Hand - microbiology ; Humans ; Infant, Newborn ; Intensive Care Units, Neonatal ; Italy - epidemiology ; Microbiology ; Miscellaneous ; Molecular Epidemiology ; Nurses ; Polymerase Chain Reaction - methods ; Repetitive Sequences, Nucleic Acid ; Serratia Infections - epidemiology ; Serratia Infections - microbiology ; Serratia marcescens - classification ; Serratia marcescens - genetics ; Serratia marcescens - isolation & purification</subject><ispartof>Journal of Clinical Microbiology, 2010-05, Vol.48 (5), p.1690-1695</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010, American Society for Microbiology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-74a6d991ab8061da633566c70b804bb9c1342f18a655b6c6be68c964c23775bc3</citedby><cites>FETCH-LOGICAL-c463t-74a6d991ab8061da633566c70b804bb9c1342f18a655b6c6be68c964c23775bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2863922/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2863922/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,3187,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22753473$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20237095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ligozzi, Marco</creatorcontrib><creatorcontrib>Fontana, Roberta</creatorcontrib><creatorcontrib>Aldegheri, Marco</creatorcontrib><creatorcontrib>Scalet, Giovanna</creatorcontrib><creatorcontrib>Lo Cascio, Giuliana</creatorcontrib><title>Comparative Evaluation of an Automated Repetitive-Sequence-Based PCR Instrument versus Pulsed-Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>A semiautomated, repetitive-sequence-based PCR (rep-PCR) instrument (DiversiLab system) was evaluated in comparison with pulsed-field gel electrophoresis (PFGE) to investigate an outbreak of Serratia marcescens infections in a neonatal intensive care unit (NICU). A selection of 36 epidemiologically related and 8 epidemiologically unrelated isolates was analyzed. Among the epidemiologically related isolates, PFGE identified five genetically unrelated patterns. Thirty-two isolates from patients and wet nurses showed the same PFGE profile (pattern A). Genetically unrelated PFGE patterns were found in one patient (pattern B), in two wet nurses (patterns C and D), and in an environmental isolate from the NICU (pattern G). Rep-PCR identified seven different patterns, three of which included the 32 isolates of PFGE type A. One or two band differences in isolates of these three types allowed isolates to be categorized as similar and included in a unique cluster. Isolates of different PFGE types were also of unrelated rep-PCR types. All of the epidemiologically unrelated isolates were of different PFGE and rep-PCR types. The level of discrimination exhibited by rep-PCR with the DiversiLab system allowed us to conclude that this method was able to identify genetic similarity in a spatio-temporal cluster of S. marcescens isolates.</description><subject>Adult</subject><subject>Automation - methods</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cluster Analysis</subject><subject>Cross Infection - epidemiology</subject><subject>Cross Infection - microbiology</subject><subject>Disease Outbreaks</subject><subject>DNA, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Environmental Microbiology</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Hand - microbiology</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Intensive Care Units, Neonatal</subject><subject>Italy - epidemiology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>Nurses</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Serratia Infections - epidemiology</subject><subject>Serratia Infections - microbiology</subject><subject>Serratia marcescens - classification</subject><subject>Serratia marcescens - genetics</subject><subject>Serratia marcescens - isolation & purification</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkV9v0zAUxS0EYqPwxjOYB8QLGf6TOMkL0qi6MTTYtDKJN-vGvWm9JXGxnSI-HN8Ndy0Dnmzr_nTO8T2EPOfsiHNRvfs0_XzEeCGqjNUPyCFndZUpxb49JIeM1UXGuSwPyJMQbhjjeV4Uj8mBYEKWaXhIfk1dvwYP0W6QzjbQjenqBupaCgM9HqPrIeKCXuEao91S2Ry_jzgYzD5ASJPL6RU9G0L0Y49DpBv0YQz0cuzSMDux2C3oKXZ01qGJ3q1XzmOwgdqBxhXSOcZoh-WdX3r4bRKgPXiDweAQ6BcXnHG9hS65tEljm-5ijI1HuH1KHrWQjJ7tzwm5Ppl9nX7Mzi9Oz6bH55nJlYxZmYNa1DWHpmKKL0BJWShlSpbeedPUhstctLwCVRSNMqpBVZla5SZtqSwaIyfk_U53PTY9LlKw6KHTa29T0p_agdX_Twa70ku30aJSshYiCbzZC3iXthei7m36X9fBgG4MukyJhBKMJfLtjjTeheCxvXfhTG8L16lwfVe4ZnXCX_yb7B7-03ACXu8BCAa61sNgbPjLibKQebKfkFc7bmWXqx_Wo4bQ6xvT67zSheaq3mZ7uWNacBqWPulczwXjkvFKVnlRy98LcMwb</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Ligozzi, Marco</creator><creator>Fontana, Roberta</creator><creator>Aldegheri, Marco</creator><creator>Scalet, Giovanna</creator><creator>Lo Cascio, Giuliana</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20100501</creationdate><title>Comparative Evaluation of an Automated Repetitive-Sequence-Based PCR Instrument versus Pulsed-Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak</title><author>Ligozzi, Marco ; Fontana, Roberta ; Aldegheri, Marco ; Scalet, Giovanna ; Lo Cascio, Giuliana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-74a6d991ab8061da633566c70b804bb9c1342f18a655b6c6be68c964c23775bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Automation - methods</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cluster Analysis</topic><topic>Cross Infection - epidemiology</topic><topic>Cross Infection - microbiology</topic><topic>Disease Outbreaks</topic><topic>DNA, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field - methods</topic><topic>Environmental Microbiology</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Hand - microbiology</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Intensive Care Units, Neonatal</topic><topic>Italy - epidemiology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>Nurses</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Repetitive Sequences, Nucleic Acid</topic><topic>Serratia Infections - epidemiology</topic><topic>Serratia Infections - microbiology</topic><topic>Serratia marcescens - classification</topic><topic>Serratia marcescens - genetics</topic><topic>Serratia marcescens - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ligozzi, Marco</creatorcontrib><creatorcontrib>Fontana, Roberta</creatorcontrib><creatorcontrib>Aldegheri, Marco</creatorcontrib><creatorcontrib>Scalet, Giovanna</creatorcontrib><creatorcontrib>Lo Cascio, Giuliana</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ligozzi, Marco</au><au>Fontana, Roberta</au><au>Aldegheri, Marco</au><au>Scalet, Giovanna</au><au>Lo Cascio, Giuliana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Evaluation of an Automated Repetitive-Sequence-Based PCR Instrument versus Pulsed-Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>48</volume><issue>5</issue><spage>1690</spage><epage>1695</epage><pages>1690-1695</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>A semiautomated, repetitive-sequence-based PCR (rep-PCR) instrument (DiversiLab system) was evaluated in comparison with pulsed-field gel electrophoresis (PFGE) to investigate an outbreak of Serratia marcescens infections in a neonatal intensive care unit (NICU). A selection of 36 epidemiologically related and 8 epidemiologically unrelated isolates was analyzed. Among the epidemiologically related isolates, PFGE identified five genetically unrelated patterns. Thirty-two isolates from patients and wet nurses showed the same PFGE profile (pattern A). Genetically unrelated PFGE patterns were found in one patient (pattern B), in two wet nurses (patterns C and D), and in an environmental isolate from the NICU (pattern G). Rep-PCR identified seven different patterns, three of which included the 32 isolates of PFGE type A. One or two band differences in isolates of these three types allowed isolates to be categorized as similar and included in a unique cluster. Isolates of different PFGE types were also of unrelated rep-PCR types. All of the epidemiologically unrelated isolates were of different PFGE and rep-PCR types. The level of discrimination exhibited by rep-PCR with the DiversiLab system allowed us to conclude that this method was able to identify genetic similarity in a spatio-temporal cluster of S. marcescens isolates.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20237095</pmid><doi>10.1128/JCM.01528-09</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Automation - methods Bacterial Typing Techniques - methods Bacteriology Biological and medical sciences Cluster Analysis Cross Infection - epidemiology Cross Infection - microbiology Disease Outbreaks DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field - methods Environmental Microbiology Epidemiology Fundamental and applied biological sciences. Psychology Genotype Hand - microbiology Humans Infant, Newborn Intensive Care Units, Neonatal Italy - epidemiology Microbiology Miscellaneous Molecular Epidemiology Nurses Polymerase Chain Reaction - methods Repetitive Sequences, Nucleic Acid Serratia Infections - epidemiology Serratia Infections - microbiology Serratia marcescens - classification Serratia marcescens - genetics Serratia marcescens - isolation & purification |
| title | Comparative Evaluation of an Automated Repetitive-Sequence-Based PCR Instrument versus Pulsed-Field Gel Electrophoresis in the Setting of a Serratia marcescens Nosocomial Infection Outbreak |
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