Sensitive quantification of bulleyaconitine A in human serum by liquid chromatography-tandem mass spectrometry
A simple and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of bulleyaconitine A (BLA) in human serum. Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Ch...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-07, Vol.73 (7), p.1572-1577 |
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| creator | Xiong, X.(Peking Univ., Beijing (China)) Zhai, S Yao, Z |
| description | A simple and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of bulleyaconitine A (BLA) in human serum. Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Chromatography separation was performed on a Waters Sunfire C18 column (2.1×50 mm, 5 μm) with a gradient mobile phase. Detection was performed in positive mode using multiple reaction monitoring (MRM) of the transition m⁄z 644.2>584.3 for BLA and m⁄z 426.3>175.3 for I.S. The proposed method was validated in a linear range of 0.0587-11.7 ng/ml. Intra- and inter-day precision was better than 9.88% and 7.76%. The recovery for BLA was 97.90%, and for I.S. 89.40%. This method provides a simple, rapid, specific, and sensitive tool for the quantitativedetermination of BLA in human serum, and can be used to monitor serum levels in patients treated with BLA. |
| doi_str_mv | 10.1271/bbb.90064 |
| format | Article |
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Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Chromatography separation was performed on a Waters Sunfire C18 column (2.1×50 mm, 5 μm) with a gradient mobile phase. Detection was performed in positive mode using multiple reaction monitoring (MRM) of the transition m⁄z 644.2>584.3 for BLA and m⁄z 426.3>175.3 for I.S. The proposed method was validated in a linear range of 0.0587-11.7 ng/ml. Intra- and inter-day precision was better than 9.88% and 7.76%. The recovery for BLA was 97.90%, and for I.S. 89.40%. This method provides a simple, rapid, specific, and sensitive tool for the quantitativedetermination of BLA in human serum, and can be used to monitor serum levels in patients treated with BLA.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.90064</identifier><identifier>PMID: 19584546</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Aconitine - administration & dosage ; Aconitine - analogs & derivatives ; Aconitine - blood ; Aconitine - chemistry ; Aconitine - therapeutic use ; ACONITUM ; ALCALOIDE ; ALCALOIDES ; ALKALOIDS ; Analgesics - administration & dosage ; Analgesics - blood ; Analgesics - chemistry ; Analgesics - therapeutic use ; ANALISIS CUANTITATIVO ; ANALYSE QUANTITATIVE ; Biological and medical sciences ; Blood Chemical Analysis - methods ; BLOOD SERUM ; bulleyaconitine A ; Chemical Precipitation ; Chromatography, Liquid ; DRUGS ; ESPECTROMETRIA DE MASAS ; Fundamental and applied biological sciences. Psychology ; HPLC ; human serum ; Humans ; Linear Models ; liquid chromatography-tandem mass spectrometry ; MASS SPECTROMETRY ; MEDICAMENT ; MEDICAMENTOS ; Osteoarthritis - blood ; Osteoarthritis - drug therapy ; Proteins - chemistry ; QUANTITATIVE ANALYSIS ; Reproducibility of Results ; Sensitivity and Specificity ; SERUM SANGUIN ; SPECTROMETRIE DE MASSE ; SUERO SANGUINEO ; Tandem Mass Spectrometry ; Time Factors</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2009-07, Vol.73 (7), p.1572-1577</ispartof><rights>2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-3b684cd060d5893f83b2531abfb9a0695b539bd2be1bfc13bd2c586464be241a3</citedby><cites>FETCH-LOGICAL-c692t-3b684cd060d5893f83b2531abfb9a0695b539bd2be1bfc13bd2c586464be241a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21884981$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19584546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiong, X.(Peking Univ., Beijing (China))</creatorcontrib><creatorcontrib>Zhai, S</creatorcontrib><creatorcontrib>Yao, Z</creatorcontrib><title>Sensitive quantification of bulleyaconitine A in human serum by liquid chromatography-tandem mass spectrometry</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>A simple and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of bulleyaconitine A (BLA) in human serum. Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Chromatography separation was performed on a Waters Sunfire C18 column (2.1×50 mm, 5 μm) with a gradient mobile phase. Detection was performed in positive mode using multiple reaction monitoring (MRM) of the transition m⁄z 644.2>584.3 for BLA and m⁄z 426.3>175.3 for I.S. The proposed method was validated in a linear range of 0.0587-11.7 ng/ml. Intra- and inter-day precision was better than 9.88% and 7.76%. The recovery for BLA was 97.90%, and for I.S. 89.40%. This method provides a simple, rapid, specific, and sensitive tool for the quantitativedetermination of BLA in human serum, and can be used to monitor serum levels in patients treated with BLA.</description><subject>Aconitine - administration & dosage</subject><subject>Aconitine - analogs & derivatives</subject><subject>Aconitine - blood</subject><subject>Aconitine - chemistry</subject><subject>Aconitine - therapeutic use</subject><subject>ACONITUM</subject><subject>ALCALOIDE</subject><subject>ALCALOIDES</subject><subject>ALKALOIDS</subject><subject>Analgesics - administration & dosage</subject><subject>Analgesics - blood</subject><subject>Analgesics - chemistry</subject><subject>Analgesics - therapeutic use</subject><subject>ANALISIS CUANTITATIVO</subject><subject>ANALYSE QUANTITATIVE</subject><subject>Biological and medical sciences</subject><subject>Blood Chemical Analysis - methods</subject><subject>BLOOD SERUM</subject><subject>bulleyaconitine A</subject><subject>Chemical Precipitation</subject><subject>Chromatography, Liquid</subject><subject>DRUGS</subject><subject>ESPECTROMETRIA DE MASAS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HPLC</subject><subject>human serum</subject><subject>Humans</subject><subject>Linear Models</subject><subject>liquid chromatography-tandem mass spectrometry</subject><subject>MASS SPECTROMETRY</subject><subject>MEDICAMENT</subject><subject>MEDICAMENTOS</subject><subject>Osteoarthritis - blood</subject><subject>Osteoarthritis - drug therapy</subject><subject>Proteins - chemistry</subject><subject>QUANTITATIVE ANALYSIS</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>SERUM SANGUIN</subject><subject>SPECTROMETRIE DE MASSE</subject><subject>SUERO SANGUINEO</subject><subject>Tandem Mass Spectrometry</subject><subject>Time Factors</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90l2LFSEYB3CJot1OXfQBCmGp6GI2HV9GL5elVxYKquvh0XH2uMzoOeoU8-1zO6eCiK6Ux9_ziPxF6DEl57Tt6CtjzLkmRPI76JQy3jVS8-4uOiWaykZxQU_Qg5xvCKkFQe-jE6pFLXN5isJnF7Iv_pvD-wVC8aO3UHwMOI7YLNPkVrAxVBEcvsA-4O0yQ8DZpWXGZsWT3y9-wHab4gwlXifYbdemQBjcjGfIGeeds6WeupLWh-jeCFN2j47rBn198_rL5bvm6uPb95cXV42Vui0NM1JxOxBJBqE0GxUzrWAUzGg0EKmFEUyboTWOmtFSVrdWKMklN67lFNgGvTjM3aW4X1wu_eyzddMEwcUl9x1jQqqOiCqf_1e2pGUtlbfw7C94E5cU6it6yjvJFOOVbtDLg7Ip5pzc2O-SnyGtPSX9bVh9Dav_GVa1T48TFzO74Y88plPBsyOAbGEaEwTr82_XUqW4VrQ6fnA-jDHN8D2maegLrFNMv5rYv-5_cmgbIfZwnar68Kmtn4QQQbViPwDafrgq</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Xiong, X.(Peking Univ., Beijing (China))</creator><creator>Zhai, S</creator><creator>Yao, Z</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090701</creationdate><title>Sensitive quantification of bulleyaconitine A in human serum by liquid chromatography-tandem mass spectrometry</title><author>Xiong, X.(Peking Univ., Beijing (China)) ; Zhai, S ; Yao, Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-3b684cd060d5893f83b2531abfb9a0695b539bd2be1bfc13bd2c586464be241a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Aconitine - administration & dosage</topic><topic>Aconitine - analogs & derivatives</topic><topic>Aconitine - blood</topic><topic>Aconitine - chemistry</topic><topic>Aconitine - therapeutic use</topic><topic>ACONITUM</topic><topic>ALCALOIDE</topic><topic>ALCALOIDES</topic><topic>ALKALOIDS</topic><topic>Analgesics - administration & dosage</topic><topic>Analgesics - blood</topic><topic>Analgesics - chemistry</topic><topic>Analgesics - therapeutic use</topic><topic>ANALISIS CUANTITATIVO</topic><topic>ANALYSE QUANTITATIVE</topic><topic>Biological and medical sciences</topic><topic>Blood Chemical Analysis - methods</topic><topic>BLOOD SERUM</topic><topic>bulleyaconitine A</topic><topic>Chemical Precipitation</topic><topic>Chromatography, Liquid</topic><topic>DRUGS</topic><topic>ESPECTROMETRIA DE MASAS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HPLC</topic><topic>human serum</topic><topic>Humans</topic><topic>Linear Models</topic><topic>liquid chromatography-tandem mass spectrometry</topic><topic>MASS SPECTROMETRY</topic><topic>MEDICAMENT</topic><topic>MEDICAMENTOS</topic><topic>Osteoarthritis - blood</topic><topic>Osteoarthritis - drug therapy</topic><topic>Proteins - chemistry</topic><topic>QUANTITATIVE ANALYSIS</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>SERUM SANGUIN</topic><topic>SPECTROMETRIE DE MASSE</topic><topic>SUERO SANGUINEO</topic><topic>Tandem Mass Spectrometry</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiong, X.(Peking Univ., Beijing (China))</creatorcontrib><creatorcontrib>Zhai, S</creatorcontrib><creatorcontrib>Yao, Z</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiong, X.(Peking Univ., Beijing (China))</au><au>Zhai, S</au><au>Yao, Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive quantification of bulleyaconitine A in human serum by liquid chromatography-tandem mass spectrometry</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>73</volume><issue>7</issue><spage>1572</spage><epage>1577</epage><pages>1572-1577</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>A simple and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the quantification of bulleyaconitine A (BLA) in human serum. Samples were extracted from human serum by protein precipitation. Domperidone was used as internal standard (I.S.). Chromatography separation was performed on a Waters Sunfire C18 column (2.1×50 mm, 5 μm) with a gradient mobile phase. Detection was performed in positive mode using multiple reaction monitoring (MRM) of the transition m⁄z 644.2>584.3 for BLA and m⁄z 426.3>175.3 for I.S. The proposed method was validated in a linear range of 0.0587-11.7 ng/ml. Intra- and inter-day precision was better than 9.88% and 7.76%. The recovery for BLA was 97.90%, and for I.S. 89.40%. This method provides a simple, rapid, specific, and sensitive tool for the quantitativedetermination of BLA in human serum, and can be used to monitor serum levels in patients treated with BLA.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>19584546</pmid><doi>10.1271/bbb.90064</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2009-07, Vol.73 (7), p.1572-1577 |
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| recordid | cdi_pubmed_primary_19584546 |
| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Aconitine - administration & dosage Aconitine - analogs & derivatives Aconitine - blood Aconitine - chemistry Aconitine - therapeutic use ACONITUM ALCALOIDE ALCALOIDES ALKALOIDS Analgesics - administration & dosage Analgesics - blood Analgesics - chemistry Analgesics - therapeutic use ANALISIS CUANTITATIVO ANALYSE QUANTITATIVE Biological and medical sciences Blood Chemical Analysis - methods BLOOD SERUM bulleyaconitine A Chemical Precipitation Chromatography, Liquid DRUGS ESPECTROMETRIA DE MASAS Fundamental and applied biological sciences. Psychology HPLC human serum Humans Linear Models liquid chromatography-tandem mass spectrometry MASS SPECTROMETRY MEDICAMENT MEDICAMENTOS Osteoarthritis - blood Osteoarthritis - drug therapy Proteins - chemistry QUANTITATIVE ANALYSIS Reproducibility of Results Sensitivity and Specificity SERUM SANGUIN SPECTROMETRIE DE MASSE SUERO SANGUINEO Tandem Mass Spectrometry Time Factors |
| title | Sensitive quantification of bulleyaconitine A in human serum by liquid chromatography-tandem mass spectrometry |
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