Improved cartilage regeneration utilizing mesenchymal stem cells in TGF-beta1 gene-activated scaffolds
Recently, bone marrow-derived mesenchymal stem cells (MSCs) have been paid more attention for cartilage regeneration. This study evaluated the potential of using MSCs seeded in plasmid transforming growth factor beta1 (pTGF-beta1)-activated three-dimensional chitosan/gelatin scaffolds for improving...
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| Veröffentlicht in: | Tissue engineering. Part A 2009-09, Vol.15 (9), p.2687 |
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| container_title | Tissue engineering. Part A |
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| creator | Diao, Huajia Wang, Jinliang Shen, Chao Xia, Suhua Guo, Ting Dong, Lei Zhang, Chenyu Chen, Jiangning Zhao, Jianning Zhang, Junfeng |
| description | Recently, bone marrow-derived mesenchymal stem cells (MSCs) have been paid more attention for cartilage regeneration. This study evaluated the potential of using MSCs seeded in plasmid transforming growth factor beta1 (pTGF-beta1)-activated three-dimensional chitosan/gelatin scaffolds for improving cartilage repair in vivo. Significant cell proliferation and transforming growth factor beta1 protein expression were observed in vitro in pTGFbeta1-activated scaffolds. Transforming growth factor beta1-activated scaffolds showed high collagen type II and aggrecan expression and low collagen type I expression during in vitro cultivation. MSC-based pTGF-beta1-activated scaffolds also exhibited cartilage histology with high secretion of collagen type II in vitro under the stimulation of pTGF-beta1. In rabbits with full-thickness cartilage defects, the implantation of MSC-based pTGF-beta1-activated scaffolds not only significantly promoted chondrogenic differentiation of MSCs and hyalin-like cartilage matrix synthesis, but also remarkably improved the overall repair of rabbit cartilage defects and exhibited favorable tissue integrity at 10 weeks postsurgery. These results suggest that MSC-based localized pTGF-beta1-activated scaffolds have potential applications for in vivo cartilage repair. |
| doi_str_mv | 10.1089/ten.tea.2008.0621 |
| format | Article |
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This study evaluated the potential of using MSCs seeded in plasmid transforming growth factor beta1 (pTGF-beta1)-activated three-dimensional chitosan/gelatin scaffolds for improving cartilage repair in vivo. Significant cell proliferation and transforming growth factor beta1 protein expression were observed in vitro in pTGFbeta1-activated scaffolds. Transforming growth factor beta1-activated scaffolds showed high collagen type II and aggrecan expression and low collagen type I expression during in vitro cultivation. MSC-based pTGF-beta1-activated scaffolds also exhibited cartilage histology with high secretion of collagen type II in vitro under the stimulation of pTGF-beta1. In rabbits with full-thickness cartilage defects, the implantation of MSC-based pTGF-beta1-activated scaffolds not only significantly promoted chondrogenic differentiation of MSCs and hyalin-like cartilage matrix synthesis, but also remarkably improved the overall repair of rabbit cartilage defects and exhibited favorable tissue integrity at 10 weeks postsurgery. These results suggest that MSC-based localized pTGF-beta1-activated scaffolds have potential applications for in vivo cartilage repair.</description><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2008.0621</identifier><identifier>PMID: 19216641</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Cartilage - drug effects ; Cartilage - physiology ; Cartilage, Articular - drug effects ; Cartilage, Articular - pathology ; Cattle ; Cell Proliferation - drug effects ; Chitosan - pharmacology ; Collagen Type II - metabolism ; Enzyme-Linked Immunosorbent Assay ; Gelatin - pharmacology ; Gene Expression Regulation - drug effects ; Green Fluorescent Proteins - metabolism ; Hyaline Cartilage - drug effects ; Hyaline Cartilage - metabolism ; Hyaline Cartilage - pathology ; Immunohistochemistry ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Mesenchymal Stromal Cells - ultrastructure ; Organ Specificity - drug effects ; Organ Specificity - genetics ; Rabbits ; Regeneration - drug effects ; Regeneration - physiology ; Tissue Scaffolds - chemistry ; Transforming Growth Factor beta1 - genetics ; Wound Healing - drug effects</subject><ispartof>Tissue engineering. 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Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Recently, bone marrow-derived mesenchymal stem cells (MSCs) have been paid more attention for cartilage regeneration. This study evaluated the potential of using MSCs seeded in plasmid transforming growth factor beta1 (pTGF-beta1)-activated three-dimensional chitosan/gelatin scaffolds for improving cartilage repair in vivo. Significant cell proliferation and transforming growth factor beta1 protein expression were observed in vitro in pTGFbeta1-activated scaffolds. Transforming growth factor beta1-activated scaffolds showed high collagen type II and aggrecan expression and low collagen type I expression during in vitro cultivation. MSC-based pTGF-beta1-activated scaffolds also exhibited cartilage histology with high secretion of collagen type II in vitro under the stimulation of pTGF-beta1. In rabbits with full-thickness cartilage defects, the implantation of MSC-based pTGF-beta1-activated scaffolds not only significantly promoted chondrogenic differentiation of MSCs and hyalin-like cartilage matrix synthesis, but also remarkably improved the overall repair of rabbit cartilage defects and exhibited favorable tissue integrity at 10 weeks postsurgery. 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diao, Huajia</au><au>Wang, Jinliang</au><au>Shen, Chao</au><au>Xia, Suhua</au><au>Guo, Ting</au><au>Dong, Lei</au><au>Zhang, Chenyu</au><au>Chen, Jiangning</au><au>Zhao, Jianning</au><au>Zhang, Junfeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved cartilage regeneration utilizing mesenchymal stem cells in TGF-beta1 gene-activated scaffolds</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2009-09</date><risdate>2009</risdate><volume>15</volume><issue>9</issue><spage>2687</spage><pages>2687-</pages><eissn>1937-335X</eissn><abstract>Recently, bone marrow-derived mesenchymal stem cells (MSCs) have been paid more attention for cartilage regeneration. This study evaluated the potential of using MSCs seeded in plasmid transforming growth factor beta1 (pTGF-beta1)-activated three-dimensional chitosan/gelatin scaffolds for improving cartilage repair in vivo. Significant cell proliferation and transforming growth factor beta1 protein expression were observed in vitro in pTGFbeta1-activated scaffolds. Transforming growth factor beta1-activated scaffolds showed high collagen type II and aggrecan expression and low collagen type I expression during in vitro cultivation. MSC-based pTGF-beta1-activated scaffolds also exhibited cartilage histology with high secretion of collagen type II in vitro under the stimulation of pTGF-beta1. In rabbits with full-thickness cartilage defects, the implantation of MSC-based pTGF-beta1-activated scaffolds not only significantly promoted chondrogenic differentiation of MSCs and hyalin-like cartilage matrix synthesis, but also remarkably improved the overall repair of rabbit cartilage defects and exhibited favorable tissue integrity at 10 weeks postsurgery. These results suggest that MSC-based localized pTGF-beta1-activated scaffolds have potential applications for in vivo cartilage repair.</abstract><cop>United States</cop><pmid>19216641</pmid><doi>10.1089/ten.tea.2008.0621</doi></addata></record> |
| fulltext | fulltext |
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| ispartof | Tissue engineering. Part A, 2009-09, Vol.15 (9), p.2687 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cartilage - drug effects Cartilage - physiology Cartilage, Articular - drug effects Cartilage, Articular - pathology Cattle Cell Proliferation - drug effects Chitosan - pharmacology Collagen Type II - metabolism Enzyme-Linked Immunosorbent Assay Gelatin - pharmacology Gene Expression Regulation - drug effects Green Fluorescent Proteins - metabolism Hyaline Cartilage - drug effects Hyaline Cartilage - metabolism Hyaline Cartilage - pathology Immunohistochemistry Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Mesenchymal Stromal Cells - ultrastructure Organ Specificity - drug effects Organ Specificity - genetics Rabbits Regeneration - drug effects Regeneration - physiology Tissue Scaffolds - chemistry Transforming Growth Factor beta1 - genetics Wound Healing - drug effects |
| title | Improved cartilage regeneration utilizing mesenchymal stem cells in TGF-beta1 gene-activated scaffolds |
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