Methanolic extract of Teucrium polium L. potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines
A growing body of evidence indicates that specific phytochemicals can increase an efficacy of cancer chemotherapy. To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubicin in...
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| Veröffentlicht in: | Experimental oncology 2008-06, Vol.30 (2), p.133 |
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| creator | Rajabalian, S |
| description | A growing body of evidence indicates that specific phytochemicals can increase an efficacy of cancer chemotherapy.
To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubicin in vitro.
Skmel-3, Saos-2, SW480, MCF-7, KB, EJ and A431 cell lines were used. Cytotoxicity was determined by MTT assay and colony formation assay. The nature of interactions was analyzed by combination index (CI) method. Apoptotic cells were assessed by DAPI staining.
The vincristine/Me-TP, vinblastine/Me-TP and doxorubicin/Me-TP combinations showed a strong synergistic effect in the cell growth inhibition (0.13 |
| format | Article |
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To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubicin in vitro.
Skmel-3, Saos-2, SW480, MCF-7, KB, EJ and A431 cell lines were used. Cytotoxicity was determined by MTT assay and colony formation assay. The nature of interactions was analyzed by combination index (CI) method. Apoptotic cells were assessed by DAPI staining.
The vincristine/Me-TP, vinblastine/Me-TP and doxorubicin/Me-TP combinations showed a strong synergistic effect in the cell growth inhibition (0.13<CI<0.36). Similar results were observed by colony formation assay. Furthermore, the combinations of vincristine/Me-TP and vinblastine/Me-TP resulted in a massive apoptosis (>80%) compared to effect of individual drugs (0-3%). At the additional experiments, Me-TP reduced marginally to significantly the cytotoxic effects of vincristine and vinblastine toward the human fibroblasts (p<0.05 to 0.001).
The present study suggests that Me-TP has the potential to be an effective and safe chemosensitizer agent for cancer therapy.</description><identifier>ISSN: 1812-9269</identifier><identifier>PMID: 18566577</identifier><language>eng</language><publisher>Ukraine</publisher><subject>Antineoplastic Agents - pharmacology ; Antineoplastic Combined Chemotherapy Protocols - therapeutic use ; Apoptosis ; Cell Line, Tumor ; Dose-Response Relationship, Drug ; Doxorubicin - administration & dosage ; Drug Screening Assays, Antitumor ; Humans ; Inhibitory Concentration 50 ; Plant Extracts - metabolism ; Teucrium - metabolism ; Vinblastine - administration & dosage ; Vincristine - administration & dosage</subject><ispartof>Experimental oncology, 2008-06, Vol.30 (2), p.133</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18566577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rajabalian, S</creatorcontrib><title>Methanolic extract of Teucrium polium L. potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines</title><title>Experimental oncology</title><addtitle>Exp Oncol</addtitle><description>A growing body of evidence indicates that specific phytochemicals can increase an efficacy of cancer chemotherapy.
To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubicin in vitro.
Skmel-3, Saos-2, SW480, MCF-7, KB, EJ and A431 cell lines were used. Cytotoxicity was determined by MTT assay and colony formation assay. The nature of interactions was analyzed by combination index (CI) method. Apoptotic cells were assessed by DAPI staining.
The vincristine/Me-TP, vinblastine/Me-TP and doxorubicin/Me-TP combinations showed a strong synergistic effect in the cell growth inhibition (0.13<CI<0.36). Similar results were observed by colony formation assay. Furthermore, the combinations of vincristine/Me-TP and vinblastine/Me-TP resulted in a massive apoptosis (>80%) compared to effect of individual drugs (0-3%). At the additional experiments, Me-TP reduced marginally to significantly the cytotoxic effects of vincristine and vinblastine toward the human fibroblasts (p<0.05 to 0.001).
The present study suggests that Me-TP has the potential to be an effective and safe chemosensitizer agent for cancer therapy.</description><subject>Antineoplastic Agents - pharmacology</subject><subject>Antineoplastic Combined Chemotherapy Protocols - therapeutic use</subject><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>Dose-Response Relationship, Drug</subject><subject>Doxorubicin - administration & dosage</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Humans</subject><subject>Inhibitory Concentration 50</subject><subject>Plant Extracts - metabolism</subject><subject>Teucrium - metabolism</subject><subject>Vinblastine - administration & dosage</subject><subject>Vincristine - administration & dosage</subject><issn>1812-9269</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kM1OAyEUhWehsbX6CoYHcAwzFAaWpvEvqXHTfXOHgRZDgQBj6tv5aDKtrs49997zLc5FNW9409aiZWJWXaf0iTGjgi2vqlnDKWO06-bVz7vKe3DeGonUMUeQGXmNNmqU0YwHFMqlyPqhTFm5bCCrhPJeIfmdffbHkgM3IAg-FD9RtFYyp4kC5V-CkyqiIY670-7LuEJO2Th1P5newsmcKIM_-jj2RhqHYAfGpYwABXDKTtkzy48JSWUtsiWWbqpLDTap2z9dVJvnp83qtV5_vLytHtd1aNplrvsWi4H3LZWMKI2XWEvM9UA71jBeesESBBOs4Z1oOSEDE5RIQhlnmFDakkV1d8aGsT-oYRuiOUD83v43SX4BmKpy4g</recordid><startdate>200806</startdate><enddate>200806</enddate><creator>Rajabalian, S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>200806</creationdate><title>Methanolic extract of Teucrium polium L. potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines</title><author>Rajabalian, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p124t-b209d8b25c63ef040fc08fd5761680650ca969618792833d6953c35686035523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antineoplastic Agents - pharmacology</topic><topic>Antineoplastic Combined Chemotherapy Protocols - therapeutic use</topic><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>Dose-Response Relationship, Drug</topic><topic>Doxorubicin - administration & dosage</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Humans</topic><topic>Inhibitory Concentration 50</topic><topic>Plant Extracts - metabolism</topic><topic>Teucrium - metabolism</topic><topic>Vinblastine - administration & dosage</topic><topic>Vincristine - administration & dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajabalian, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Experimental oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajabalian, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methanolic extract of Teucrium polium L. potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines</atitle><jtitle>Experimental oncology</jtitle><addtitle>Exp Oncol</addtitle><date>2008-06</date><risdate>2008</risdate><volume>30</volume><issue>2</issue><spage>133</spage><pages>133-</pages><issn>1812-9269</issn><abstract>A growing body of evidence indicates that specific phytochemicals can increase an efficacy of cancer chemotherapy.
To evaluate promoting effect of the methanolic extract of Teucrium Polium (Me-TP) on cytotoxic and apoptotic activity of anticancer drugs of vinblastine, vincristine and doxorubicin in vitro.
Skmel-3, Saos-2, SW480, MCF-7, KB, EJ and A431 cell lines were used. Cytotoxicity was determined by MTT assay and colony formation assay. The nature of interactions was analyzed by combination index (CI) method. Apoptotic cells were assessed by DAPI staining.
The vincristine/Me-TP, vinblastine/Me-TP and doxorubicin/Me-TP combinations showed a strong synergistic effect in the cell growth inhibition (0.13<CI<0.36). Similar results were observed by colony formation assay. Furthermore, the combinations of vincristine/Me-TP and vinblastine/Me-TP resulted in a massive apoptosis (>80%) compared to effect of individual drugs (0-3%). At the additional experiments, Me-TP reduced marginally to significantly the cytotoxic effects of vincristine and vinblastine toward the human fibroblasts (p<0.05 to 0.001).
The present study suggests that Me-TP has the potential to be an effective and safe chemosensitizer agent for cancer therapy.</abstract><cop>Ukraine</cop><pmid>18566577</pmid></addata></record> |
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| ispartof | Experimental oncology, 2008-06, Vol.30 (2), p.133 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antineoplastic Agents - pharmacology Antineoplastic Combined Chemotherapy Protocols - therapeutic use Apoptosis Cell Line, Tumor Dose-Response Relationship, Drug Doxorubicin - administration & dosage Drug Screening Assays, Antitumor Humans Inhibitory Concentration 50 Plant Extracts - metabolism Teucrium - metabolism Vinblastine - administration & dosage Vincristine - administration & dosage |
| title | Methanolic extract of Teucrium polium L. potentiates the cytotoxic and apoptotic effects of anticancer drugs of vincristine, vinblastine and doxorubicin against a panel of cancerous cell lines |
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