Receptor Transporter Protein 4 (RTP4) in Endometrium, Ovary, and Peripheral Blood Leukocytes of Pregnant and Cyclic Ewes
Interferon-tau (IFNT) is secreted by the conceptus trophoblast and signals pregnancy recognition in ruminants. IFNT regulates expression of genes in the endometrium, peripheral blood leukocytes (PBLs), and corpus luteum (CL). Microarray analysis identified that expression of (chemosensory) receptor...
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| Veröffentlicht in: | Biology of reproduction 2008-09, Vol.79 (3), p.518-524 |
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| creator | GIFFORD, C. A ASSIRI, A. M SATTERFIELD, M. C SPENCER, T. E OTT, T. L |
| description | Interferon-tau (IFNT) is secreted by the conceptus trophoblast and signals pregnancy recognition in ruminants. IFNT regulates
expression of genes in the endometrium, peripheral blood leukocytes (PBLs), and corpus luteum (CL). Microarray analysis identified
that expression of (chemosensory) receptor transporter protein 4 ( RTP4 ) increased in PBLs during early pregnancy in cows. In the present study, we cloned and characterized RTP4 transcription during early pregnancy in ewes. Endometrium, PBLs, and CL were collected on Days 11, 13, and 15 of the cycle
and on Days 11, 13, 15, 17, and 19 of pregnancy. Northern blot analysis revealed an expected 1.6-kb mRNA and an unexpected
2.6-kb mRNA. In endometria, RTP4 mRNA levels in cyclic ewes remained low, whereas RTP4 mRNA increased from Day 11 to Day 17 in pregnant ewes. Levels of RTP4 mRNA also increased from Day 15 to Day 19 in CL and PBL samples from pregnant ewes only. The RTP4 mRNA was located in the glandular epithelium, stratum compactum, and caruncular stroma. Ovine glandular epithelial cells
were treated with IFNT to determine if IFNT alone could induce RTP4 . IFNT increased RTP4 more than 70-fold at 1.5 h after treatment, with maximal induction of nearly 300-fold above values observed in nontreated
controls at 6 h after treatment. These results indicate that RTP4 mRNA levels are induced in the ovine endometrium, PBLs, and CL by IFNT during early pregnancy and in cell culture in response
to IFNT. If RTP4 expression affects G protein-coupled receptor function, it may be important for establishment of pregnancy
in domestic ruminants. |
| doi_str_mv | 10.1095/biolreprod.108.069468 |
| format | Article |
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expression of genes in the endometrium, peripheral blood leukocytes (PBLs), and corpus luteum (CL). Microarray analysis identified
that expression of (chemosensory) receptor transporter protein 4 ( RTP4 ) increased in PBLs during early pregnancy in cows. In the present study, we cloned and characterized RTP4 transcription during early pregnancy in ewes. Endometrium, PBLs, and CL were collected on Days 11, 13, and 15 of the cycle
and on Days 11, 13, 15, 17, and 19 of pregnancy. Northern blot analysis revealed an expected 1.6-kb mRNA and an unexpected
2.6-kb mRNA. In endometria, RTP4 mRNA levels in cyclic ewes remained low, whereas RTP4 mRNA increased from Day 11 to Day 17 in pregnant ewes. Levels of RTP4 mRNA also increased from Day 15 to Day 19 in CL and PBL samples from pregnant ewes only. The RTP4 mRNA was located in the glandular epithelium, stratum compactum, and caruncular stroma. Ovine glandular epithelial cells
were treated with IFNT to determine if IFNT alone could induce RTP4 . IFNT increased RTP4 more than 70-fold at 1.5 h after treatment, with maximal induction of nearly 300-fold above values observed in nontreated
controls at 6 h after treatment. These results indicate that RTP4 mRNA levels are induced in the ovine endometrium, PBLs, and CL by IFNT during early pregnancy and in cell culture in response
to IFNT. If RTP4 expression affects G protein-coupled receptor function, it may be important for establishment of pregnancy
in domestic ruminants.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.108.069468</identifier><identifier>PMID: 18495679</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Amino Acid Sequence ; Animals ; Biological and medical sciences ; Corpus Luteum - drug effects ; Corpus Luteum - metabolism ; Endometrium - drug effects ; Endometrium - metabolism ; Estrous Cycle - blood ; Estrous Cycle - drug effects ; Estrous Cycle - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Hormone metabolism and regulation ; Interferon Type I - pharmacology ; Leukocytes - metabolism ; Membrane Transport Proteins - blood ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Molecular Sequence Data ; Ovary - drug effects ; Ovary - metabolism ; Pregnancy - genetics ; Pregnancy - metabolism ; Pregnancy Proteins - pharmacology ; Pregnancy. Parturition. Lactation ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sheep - genetics ; Sheep - metabolism ; Tissue Distribution ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2008-09, Vol.79 (3), p.518-524</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20594879$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18495679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GIFFORD, C. A</creatorcontrib><creatorcontrib>ASSIRI, A. M</creatorcontrib><creatorcontrib>SATTERFIELD, M. C</creatorcontrib><creatorcontrib>SPENCER, T. E</creatorcontrib><creatorcontrib>OTT, T. L</creatorcontrib><title>Receptor Transporter Protein 4 (RTP4) in Endometrium, Ovary, and Peripheral Blood Leukocytes of Pregnant and Cyclic Ewes</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Interferon-tau (IFNT) is secreted by the conceptus trophoblast and signals pregnancy recognition in ruminants. IFNT regulates
expression of genes in the endometrium, peripheral blood leukocytes (PBLs), and corpus luteum (CL). Microarray analysis identified
that expression of (chemosensory) receptor transporter protein 4 ( RTP4 ) increased in PBLs during early pregnancy in cows. In the present study, we cloned and characterized RTP4 transcription during early pregnancy in ewes. Endometrium, PBLs, and CL were collected on Days 11, 13, and 15 of the cycle
and on Days 11, 13, 15, 17, and 19 of pregnancy. Northern blot analysis revealed an expected 1.6-kb mRNA and an unexpected
2.6-kb mRNA. In endometria, RTP4 mRNA levels in cyclic ewes remained low, whereas RTP4 mRNA increased from Day 11 to Day 17 in pregnant ewes. Levels of RTP4 mRNA also increased from Day 15 to Day 19 in CL and PBL samples from pregnant ewes only. The RTP4 mRNA was located in the glandular epithelium, stratum compactum, and caruncular stroma. Ovine glandular epithelial cells
were treated with IFNT to determine if IFNT alone could induce RTP4 . IFNT increased RTP4 more than 70-fold at 1.5 h after treatment, with maximal induction of nearly 300-fold above values observed in nontreated
controls at 6 h after treatment. These results indicate that RTP4 mRNA levels are induced in the ovine endometrium, PBLs, and CL by IFNT during early pregnancy and in cell culture in response
to IFNT. If RTP4 expression affects G protein-coupled receptor function, it may be important for establishment of pregnancy
in domestic ruminants.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Corpus Luteum - drug effects</subject><subject>Corpus Luteum - metabolism</subject><subject>Endometrium - drug effects</subject><subject>Endometrium - metabolism</subject><subject>Estrous Cycle - blood</subject><subject>Estrous Cycle - drug effects</subject><subject>Estrous Cycle - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Hormone metabolism and regulation</subject><subject>Interferon Type I - pharmacology</subject><subject>Leukocytes - metabolism</subject><subject>Membrane Transport Proteins - blood</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Ovary - drug effects</subject><subject>Ovary - metabolism</subject><subject>Pregnancy - genetics</subject><subject>Pregnancy - metabolism</subject><subject>Pregnancy Proteins - pharmacology</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sheep - genetics</subject><subject>Sheep - metabolism</subject><subject>Tissue Distribution</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkF1r2zAUhsVYWdK0P6FFN4MN6lZflqXLLaRbIZBQ0msjy0eNNtsyklMv_36mzUav3vPCw8PhReiKkltKdH5X-dBE6GOop65uidRCqg9oTnOms4JJ9RHNCSEy41zyGTpP6RchVHDGP6EZVULnstBz9OcRLPRDiHgXTZf6EAeIeBvDAL7DAn953G3FVzzdq64OLQzRH9obvHkx8XiDTVfjLUTf7yGaBn9vQqjxGg6_gz0OkHBwkwqeO9MNr-zyaBtv8WqEdIHOnGkSXJ5ygZ7uV7vlz2y9-fGw_LbO9kzqIctFXiipHWfK8Vo664gGARVzdSUUtZwbVylpdQ1OMCONBMeo5KqQ1FVG8QW6fvP2h6qFuuyjb6ffy38TTMDnE2CSNY2bZrA-_ecYybVQ77m9f96PPkKZWtM0k5aX4zgWuuRlThX_C026e54</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>GIFFORD, C. A</creator><creator>ASSIRI, A. M</creator><creator>SATTERFIELD, M. C</creator><creator>SPENCER, T. E</creator><creator>OTT, T. L</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20080901</creationdate><title>Receptor Transporter Protein 4 (RTP4) in Endometrium, Ovary, and Peripheral Blood Leukocytes of Pregnant and Cyclic Ewes</title><author>GIFFORD, C. A ; ASSIRI, A. M ; SATTERFIELD, M. C ; SPENCER, T. E ; OTT, T. L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h269t-5457869f328f3d6fcf09e4eb2fdb481c33afb86c9def42a6a6ef21638761fba83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Corpus Luteum - drug effects</topic><topic>Corpus Luteum - metabolism</topic><topic>Endometrium - drug effects</topic><topic>Endometrium - metabolism</topic><topic>Estrous Cycle - blood</topic><topic>Estrous Cycle - drug effects</topic><topic>Estrous Cycle - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Hormone metabolism and regulation</topic><topic>Interferon Type I - pharmacology</topic><topic>Leukocytes - metabolism</topic><topic>Membrane Transport Proteins - blood</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Ovary - drug effects</topic><topic>Ovary - metabolism</topic><topic>Pregnancy - genetics</topic><topic>Pregnancy - metabolism</topic><topic>Pregnancy Proteins - pharmacology</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sheep - genetics</topic><topic>Sheep - metabolism</topic><topic>Tissue Distribution</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GIFFORD, C. A</creatorcontrib><creatorcontrib>ASSIRI, A. M</creatorcontrib><creatorcontrib>SATTERFIELD, M. C</creatorcontrib><creatorcontrib>SPENCER, T. E</creatorcontrib><creatorcontrib>OTT, T. L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GIFFORD, C. A</au><au>ASSIRI, A. M</au><au>SATTERFIELD, M. C</au><au>SPENCER, T. E</au><au>OTT, T. L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Receptor Transporter Protein 4 (RTP4) in Endometrium, Ovary, and Peripheral Blood Leukocytes of Pregnant and Cyclic Ewes</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>79</volume><issue>3</issue><spage>518</spage><epage>524</epage><pages>518-524</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Interferon-tau (IFNT) is secreted by the conceptus trophoblast and signals pregnancy recognition in ruminants. IFNT regulates
expression of genes in the endometrium, peripheral blood leukocytes (PBLs), and corpus luteum (CL). Microarray analysis identified
that expression of (chemosensory) receptor transporter protein 4 ( RTP4 ) increased in PBLs during early pregnancy in cows. In the present study, we cloned and characterized RTP4 transcription during early pregnancy in ewes. Endometrium, PBLs, and CL were collected on Days 11, 13, and 15 of the cycle
and on Days 11, 13, 15, 17, and 19 of pregnancy. Northern blot analysis revealed an expected 1.6-kb mRNA and an unexpected
2.6-kb mRNA. In endometria, RTP4 mRNA levels in cyclic ewes remained low, whereas RTP4 mRNA increased from Day 11 to Day 17 in pregnant ewes. Levels of RTP4 mRNA also increased from Day 15 to Day 19 in CL and PBL samples from pregnant ewes only. The RTP4 mRNA was located in the glandular epithelium, stratum compactum, and caruncular stroma. Ovine glandular epithelial cells
were treated with IFNT to determine if IFNT alone could induce RTP4 . IFNT increased RTP4 more than 70-fold at 1.5 h after treatment, with maximal induction of nearly 300-fold above values observed in nontreated
controls at 6 h after treatment. These results indicate that RTP4 mRNA levels are induced in the ovine endometrium, PBLs, and CL by IFNT during early pregnancy and in cell culture in response
to IFNT. If RTP4 expression affects G protein-coupled receptor function, it may be important for establishment of pregnancy
in domestic ruminants.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>18495679</pmid><doi>10.1095/biolreprod.108.069468</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 2008-09, Vol.79 (3), p.518-524 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; BioOne Complete |
| subjects | Amino Acid Sequence Animals Biological and medical sciences Corpus Luteum - drug effects Corpus Luteum - metabolism Endometrium - drug effects Endometrium - metabolism Estrous Cycle - blood Estrous Cycle - drug effects Estrous Cycle - metabolism Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Hormone metabolism and regulation Interferon Type I - pharmacology Leukocytes - metabolism Membrane Transport Proteins - blood Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Molecular Sequence Data Ovary - drug effects Ovary - metabolism Pregnancy - genetics Pregnancy - metabolism Pregnancy Proteins - pharmacology Pregnancy. Parturition. Lactation Sequence Analysis, DNA Sequence Homology, Amino Acid Sheep - genetics Sheep - metabolism Tissue Distribution Vertebrates: reproduction |
| title | Receptor Transporter Protein 4 (RTP4) in Endometrium, Ovary, and Peripheral Blood Leukocytes of Pregnant and Cyclic Ewes |
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