Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens
The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory di...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2008-06, Vol.46 (6), p.2074-2077 |
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| container_title | Journal of Clinical Microbiology |
| container_volume | 46 |
| creator | Benson, Robert Tondella, Maria L Bhatnagar, Julu Carvalho, Maria da Glória S Sampson, Jacquelyn S Talkington, Deborah F Whitney, Anne M Mothershed, Elizabeth McGee, Lesley Carlone, George McClee, Vondguraus Guarner, Jeannette Zaki, Sherif Dejsiri, Surang Cronin, K Han, Jian Fields, Barry S |
| description | The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction. |
| doi_str_mv | 10.1128/JCM.01858-07 |
| format | Article |
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This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JCM.01858-07</identifier><identifier>PMID: 18400916</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacterial Infections - microbiology ; Bacteriology ; Biological and medical sciences ; DNA Primers ; DNA, Bacterial - analysis ; Fundamental and applied biological sciences. 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This assay was compared with real-time PCR assays based upon the same target sequences for the ability detect the target bacteria by use of both stock strains and specimens from respiratory disease patients. The ResPlex I assay is somewhat less sensitive than real-time PCR assays but offers the advantage of multiple assays in a single reaction.</description><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Infections - microbiology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reagent Kits, Diagnostic</subject><subject>Respiratory System - microbiology</subject><subject>Respiratory Tract Infections - diagnosis</subject><subject>Respiratory Tract Infections - microbiology</subject><subject>Sensitivity and Specificity</subject><issn>0095-1137</issn><issn>1098-660X</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAURiMEYsrAjjWYBazIcO04sbNBgnb40xRGw4zEzrp1nNYjJy52UuhL8My4tAywYmXpfkfH1_6y7CGFE0qZfPFhOj8BKkuZg7iVTSjUMq8q-HI7mwDUZU5pIY6yezFeA1DOy_JudkQlTxmtJtmPmdkY59ed6QeCfUNON-hGHKzviW8Jko8-5WQ-usGunflOzqcX5NLoVe-dX25J6wOZe2f06DCQmW1bE5LKoiMzMxj9W_Qa9WDCbnxh4toGHHzYJj4ajIac47DyS9PH-9mdFl00Dw7ncXb15vRy-i4_-_T2_fTVWa55XQy5ZFhQLprFAjTTpm4rYVAKIQGgqKEWctFQjVpCGqLApi5lUwOHBS1ZC7w4zl7uvetx0ZlGp5UDOrUOtsOwVR6t-jfp7Uot_UYxzispWBI8OwiC_zqaOKjORm2cw974MSpBKy6rtM3_QAbABAiawOd7UAcfYzDtzTYU1K5plZpWv5pWIBL-6O8X_IEP1Sbg6QHAqNG1AXtt4w3HgNOa0yJxT_bcyi5X32wwCmOnrnWneKWqxInddz3eMy16hcuQPFefGdAiXQUFBVb8BJ5Zx3M</recordid><startdate>20080601</startdate><enddate>20080601</enddate><creator>Benson, Robert</creator><creator>Tondella, Maria L</creator><creator>Bhatnagar, Julu</creator><creator>Carvalho, Maria da Glória S</creator><creator>Sampson, Jacquelyn S</creator><creator>Talkington, Deborah F</creator><creator>Whitney, Anne M</creator><creator>Mothershed, Elizabeth</creator><creator>McGee, Lesley</creator><creator>Carlone, George</creator><creator>McClee, Vondguraus</creator><creator>Guarner, Jeannette</creator><creator>Zaki, Sherif</creator><creator>Dejsiri, Surang</creator><creator>Cronin, K</creator><creator>Han, Jian</creator><creator>Fields, Barry S</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080601</creationdate><title>Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens</title><author>Benson, Robert ; Tondella, Maria L ; Bhatnagar, Julu ; Carvalho, Maria da Glória S ; Sampson, Jacquelyn S ; Talkington, Deborah F ; Whitney, Anne M ; Mothershed, Elizabeth ; McGee, Lesley ; Carlone, George ; McClee, Vondguraus ; Guarner, Jeannette ; Zaki, Sherif ; Dejsiri, Surang ; Cronin, K ; Han, Jian ; Fields, Barry S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-82a3147dbb0c2ce9f67ea8778000390978bd1cac80877a7ad958d9040b152f043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacterial Infections - microbiology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reagent Kits, Diagnostic</topic><topic>Respiratory System - microbiology</topic><topic>Respiratory Tract Infections - diagnosis</topic><topic>Respiratory Tract Infections - microbiology</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Benson, Robert</creatorcontrib><creatorcontrib>Tondella, Maria L</creatorcontrib><creatorcontrib>Bhatnagar, Julu</creatorcontrib><creatorcontrib>Carvalho, Maria da Glória S</creatorcontrib><creatorcontrib>Sampson, Jacquelyn S</creatorcontrib><creatorcontrib>Talkington, Deborah F</creatorcontrib><creatorcontrib>Whitney, Anne M</creatorcontrib><creatorcontrib>Mothershed, Elizabeth</creatorcontrib><creatorcontrib>McGee, Lesley</creatorcontrib><creatorcontrib>Carlone, George</creatorcontrib><creatorcontrib>McClee, Vondguraus</creatorcontrib><creatorcontrib>Guarner, Jeannette</creatorcontrib><creatorcontrib>Zaki, Sherif</creatorcontrib><creatorcontrib>Dejsiri, Surang</creatorcontrib><creatorcontrib>Cronin, K</creatorcontrib><creatorcontrib>Han, Jian</creatorcontrib><creatorcontrib>Fields, Barry S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Benson, Robert</au><au>Tondella, Maria L</au><au>Bhatnagar, Julu</au><au>Carvalho, Maria da Glória S</au><au>Sampson, Jacquelyn S</au><au>Talkington, Deborah F</au><au>Whitney, Anne M</au><au>Mothershed, Elizabeth</au><au>McGee, Lesley</au><au>Carlone, George</au><au>McClee, Vondguraus</au><au>Guarner, Jeannette</au><au>Zaki, Sherif</au><au>Dejsiri, Surang</au><au>Cronin, K</au><au>Han, Jian</au><au>Fields, Barry S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2008-06-01</date><risdate>2008</risdate><volume>46</volume><issue>6</issue><spage>2074</spage><epage>2077</epage><pages>2074-2077</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>The ResPlex I assay (Qiagen) was designed to amplify and detect DNA of six bacterial respiratory pathogens. 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| identifier | ISSN: 0095-1137 |
| ispartof | Journal of Clinical Microbiology, 2008-06, Vol.46 (6), p.2074-2077 |
| issn | 0095-1137 1098-660X 1098-5530 |
| language | eng |
| recordid | cdi_pubmed_primary_18400916 |
| source | MEDLINE; American Society for Microbiology Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacterial Infections - microbiology Bacteriology Biological and medical sciences DNA Primers DNA, Bacterial - analysis Fundamental and applied biological sciences. Psychology Humans Microbiology Polymerase Chain Reaction - methods Reagent Kits, Diagnostic Respiratory System - microbiology Respiratory Tract Infections - diagnosis Respiratory Tract Infections - microbiology Sensitivity and Specificity |
| title | Development and Evaluation of a Novel Multiplex PCR Technology for Molecular Differential Detection of Bacterial Respiratory Disease Pathogens |
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