Dynamic development of the calyx of Held synapse
The calyx of Held is probably the largest synaptic terminal in the brain, forming a unique one-to-one connection in the auditory ventral brainstem. During early development, calyces have many collaterals, whose function is unknown. Using electrophysiological recordings and fast-calcium imaging in br...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2008-04, Vol.105 (14), p.5603-5608 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Rodríguez-Contreras, Adrián van Hoeve, John Silvio Soria Habets, Ron L.P Locher, Heiko Borst, J. Gerard G |
| description | The calyx of Held is probably the largest synaptic terminal in the brain, forming a unique one-to-one connection in the auditory ventral brainstem. During early development, calyces have many collaterals, whose function is unknown. Using electrophysiological recordings and fast-calcium imaging in brain slices, we demonstrate that these collaterals are involved in synaptic transmission. We show evidence that the collaterals are pruned and that the pruning already begins 1 week before the onset of hearing. Using two-photon microscopy to image the calyx of Held in neonate rats, we report evidence that both axons and nascent calyces are structurally dynamic, showing the formation, elimination, extension, or retraction of up to 65% of their collaterals within 1 hour. The observed dynamic behavior of axons may add flexibility in the choice of postsynaptic partners and thereby contribute to ensuring that each principal cell eventually is contacted by a single calyx of Held. |
| doi_str_mv | 10.1073/pnas.0801395105 |
| format | Article |
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Gerard G</creator><creatorcontrib>Rodríguez-Contreras, Adrián ; van Hoeve, John Silvio Soria ; Habets, Ron L.P ; Locher, Heiko ; Borst, J. Gerard G</creatorcontrib><description>The calyx of Held is probably the largest synaptic terminal in the brain, forming a unique one-to-one connection in the auditory ventral brainstem. During early development, calyces have many collaterals, whose function is unknown. Using electrophysiological recordings and fast-calcium imaging in brain slices, we demonstrate that these collaterals are involved in synaptic transmission. We show evidence that the collaterals are pruned and that the pruning already begins 1 week before the onset of hearing. Using two-photon microscopy to image the calyx of Held in neonate rats, we report evidence that both axons and nascent calyces are structurally dynamic, showing the formation, elimination, extension, or retraction of up to 65% of their collaterals within 1 hour. 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Gerard G</creatorcontrib><title>Dynamic development of the calyx of Held synapse</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The calyx of Held is probably the largest synaptic terminal in the brain, forming a unique one-to-one connection in the auditory ventral brainstem. During early development, calyces have many collaterals, whose function is unknown. Using electrophysiological recordings and fast-calcium imaging in brain slices, we demonstrate that these collaterals are involved in synaptic transmission. We show evidence that the collaterals are pruned and that the pruning already begins 1 week before the onset of hearing. Using two-photon microscopy to image the calyx of Held in neonate rats, we report evidence that both axons and nascent calyces are structurally dynamic, showing the formation, elimination, extension, or retraction of up to 65% of their collaterals within 1 hour. The observed dynamic behavior of axons may add flexibility in the choice of postsynaptic partners and thereby contribute to ensuring that each principal cell eventually is contacted by a single calyx of Held.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Auditory Pathways - growth & development</subject><subject>Auditory Pathways - physiology</subject><subject>Axons</subject><subject>Axons - physiology</subject><subject>Biological Sciences</subject><subject>Brain</subject><subject>Brain stem</subject><subject>Brain Stem - growth & development</subject><subject>Brain Stem - physiology</subject><subject>Calcium</subject><subject>Calyx</subject><subject>Ears & hearing</subject><subject>Electrophysiology</subject><subject>Imaging</subject><subject>Innervation</subject><subject>Microscopy</subject><subject>Neurons</subject><subject>Neuroscience</subject><subject>Neurosciences</subject><subject>Postnatal development</subject><subject>Presynaptic Terminals - physiology</subject><subject>Rats</subject><subject>Rodents</subject><subject>Synapses</subject><subject>Synaptic Transmission</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0b9v1DAUB3ALgehRmJmAiAGxpH3-GXuphNpCkSoxQGfLiZ02JycOdlL1_nsc3ekOGNrJsvzx137vIfQWwwmGip6Og0knIAFTxTHwZ2iFQeFSMAXP0QqAVKVkhB2hVymtAUBxCS_REZa04pUQKwQXm8H0XVNYd-98GHs3TEVoi-nOFY3xm4dlc-W8LVKGY3Kv0YvW-OTe7NZjdPP18tf5VXn949v38y_XZSOwnErV1HVFFK8qJziTUlpbAW8c4NpxwmltWeuIsIQ6LCSmNbaWMi45UGJboPQYnW1zx7nunW3yv6Lxeoxdb-JGB9Ppf0-G7k7fhntNiMo9WAI-7QJi-D27NOm-S43z3gwuzElXwJTIDz0JCQghOJEZfvwPrsMch9yFbDDFklOR0ekWNTGkFF27_zIGvcxMLzPTh5nlG-__rvTgd0PK4PMOLDcPcVxjpnmuQbez95N7mDL98DjN4t1WrNMU4p4QzgQWufv7hNYEbW5jl_TNz6U8ACmpUED_AKo6u5Y</recordid><startdate>20080408</startdate><enddate>20080408</enddate><creator>Rodríguez-Contreras, Adrián</creator><creator>van Hoeve, John Silvio Soria</creator><creator>Habets, Ron L.P</creator><creator>Locher, Heiko</creator><creator>Borst, J. 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Gerard G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c618t-9cbb729577e654888dd705ce01be5253bd4fe26d23e16813b1dd34585032df033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Auditory Pathways - growth & development</topic><topic>Auditory Pathways - physiology</topic><topic>Axons</topic><topic>Axons - physiology</topic><topic>Biological Sciences</topic><topic>Brain</topic><topic>Brain stem</topic><topic>Brain Stem - growth & development</topic><topic>Brain Stem - physiology</topic><topic>Calcium</topic><topic>Calyx</topic><topic>Ears & hearing</topic><topic>Electrophysiology</topic><topic>Imaging</topic><topic>Innervation</topic><topic>Microscopy</topic><topic>Neurons</topic><topic>Neuroscience</topic><topic>Neurosciences</topic><topic>Postnatal development</topic><topic>Presynaptic Terminals - physiology</topic><topic>Rats</topic><topic>Rodents</topic><topic>Synapses</topic><topic>Synaptic Transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez-Contreras, Adrián</creatorcontrib><creatorcontrib>van Hoeve, John Silvio Soria</creatorcontrib><creatorcontrib>Habets, Ron L.P</creatorcontrib><creatorcontrib>Locher, Heiko</creatorcontrib><creatorcontrib>Borst, J. 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| subjects | Animals Animals, Newborn Auditory Pathways - growth & development Auditory Pathways - physiology Axons Axons - physiology Biological Sciences Brain Brain stem Brain Stem - growth & development Brain Stem - physiology Calcium Calyx Ears & hearing Electrophysiology Imaging Innervation Microscopy Neurons Neuroscience Neurosciences Postnatal development Presynaptic Terminals - physiology Rats Rodents Synapses Synaptic Transmission |
| title | Dynamic development of the calyx of Held synapse |
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