Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes
A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designa...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2008-05, Vol.46 (5), p.1747-1751 |
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| container_title | Journal of Clinical Microbiology |
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| creator | Gyarmati, Péter Conze, Tim Zohari, Siamak LeBlanc, Neil Nilsson, Mats Landegren, Ulf Banér, Johan Belák, Sándor |
| description | A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods. |
| doi_str_mv | 10.1128/JCM.02292-07 |
| format | Article |
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The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. 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The method uses padlock probe chemistry combined with a microarray output for detection. The outstanding feature of this assay is its capability to designate both the HA and the NA of an AIV sample from a single reaction mixture. A panel of 77 influenza virus strains was tested representing the entire assortment of the two antigens. One hundred percent (77/77) of the samples tested were identified as AIV, and 97% (75/77) were subtyped correctly in accordance with previous examinations performed by classical diagnostic methods. Testing of heterologous pathogens verified the specificity of the assay. This assay is a convenient and practical tool for the study of AIVs, providing important HA and NA data more rapidly than conventional methods.</description><subject>Animals</subject><subject>Avian influenza virus</subject><subject>Biological and medical sciences</subject><subject>DNA Primers - genetics</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Hemagglutinin Glycoproteins, Influenza Virus - genetics</topic><topic>Influenza in Birds - virology</topic><topic>Influenza virus</topic><topic>MEDICIN</topic><topic>MEDICINE</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Neuraminidase - genetics</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Orthomyxoviridae - classification</topic><topic>Orthomyxoviridae - genetics</topic><topic>Poultry</topic><topic>Sensitivity and Specificity</topic><topic>Viral Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gyarmati, Péter</creatorcontrib><creatorcontrib>Conze, Tim</creatorcontrib><creatorcontrib>Zohari, Siamak</creatorcontrib><creatorcontrib>LeBlanc, Neil</creatorcontrib><creatorcontrib>Nilsson, Mats</creatorcontrib><creatorcontrib>Landegren, Ulf</creatorcontrib><creatorcontrib>Banér, Johan</creatorcontrib><creatorcontrib>Belák, Sándor</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Uppsala universitet</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gyarmati, Péter</au><au>Conze, Tim</au><au>Zohari, Siamak</au><au>LeBlanc, Neil</au><au>Nilsson, Mats</au><au>Landegren, Ulf</au><au>Banér, Johan</au><au>Belák, Sándor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>46</volume><issue>5</issue><spage>1747</spage><epage>1751</epage><pages>1747-1751</pages><issn>0095-1137</issn><issn>1098-660X</issn><eissn>1098-660X</eissn><eissn>1098-5530</eissn><coden>JCMIDW</coden><abstract>A subtyping assay for both the hemagglutinin (HA) and neuraminidase (NA) surface antigens of the avian influenza virus (AIV) has been developed. 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| subjects | Animals Avian influenza virus Biological and medical sciences DNA Primers - genetics Fundamental and applied biological sciences. Psychology Hemagglutinin Glycoproteins, Influenza Virus - genetics Influenza in Birds - virology Influenza virus MEDICIN MEDICINE Microbiology Miscellaneous Neuraminidase - genetics Nucleic Acid Amplification Techniques - methods Oligonucleotide Array Sequence Analysis - methods Orthomyxoviridae - classification Orthomyxoviridae - genetics Poultry Sensitivity and Specificity Viral Proteins - genetics Virology |
| title | Simultaneous Genotyping of All Hemagglutinin and Neuraminidase Subtypes of Avian Influenza Viruses by Use of Padlock Probes |
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