Contribution of P2Y(1) receptors to ADP signalling in mouse spinal cord cultures
Mixed neuronal and glial cell spinal cord cultures from neonates express ADP sensitive P2Y(1,12&13) receptors. ADP (10microM) evoked increases in intracellular calcium that were essentially abolished by the P2Y(1) receptor antagonist MRS2179 (10microM), responses were also absent in preparations...
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| Veröffentlicht in: | Neuroscience letters 2008-04, Vol.435 (3), p.190 |
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| creator | Atterbury-Thomas, Amelia E Leon, Catherine Gachet, Christian Forsythe, Ian D Evans, Richard J |
| description | Mixed neuronal and glial cell spinal cord cultures from neonates express ADP sensitive P2Y(1,12&13) receptors. ADP (10microM) evoked increases in intracellular calcium that were essentially abolished by the P2Y(1) receptor antagonist MRS2179 (10microM), responses were also absent in preparations from P2Y(1) receptor deficient mice however UTP (100microM) evoked calcium rises were unaffected. ADP also evoked a robust increase in extracellular signal-regulated protein kinase (ERK) phosphorylation that was of similar magnitude in the cultures from wild type and P2Y(1) receptor deficient mice. These results suggest that ADP acts through P2Y(1) receptors to mediate an increase in intracellular calcium but not to stimulate ERK phosphorylation in the spinal cord. |
| doi_str_mv | 10.1016/j.neulet.2008.02.034 |
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| ispartof | Neuroscience letters, 2008-04, Vol.435 (3), p.190 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Diphosphate - analogs & derivatives Adenosine Diphosphate - metabolism Adenosine Diphosphate - pharmacology Aniline Compounds - metabolism Animals Calcium - metabolism Dose-Response Relationship, Drug Drug Interactions Female Male Mice Mice, Inbred C57BL Mice, Knockout Purinergic P2 Receptor Antagonists Receptors, Purinergic P2 - deficiency Receptors, Purinergic P2 - physiology Receptors, Purinergic P2Y1 Signal Transduction - drug effects Signal Transduction - physiology Spinal Cord - drug effects Spinal Cord - physiology Time Factors Tissue Culture Techniques Uridine Triphosphate - pharmacology Xanthenes - metabolism |
| title | Contribution of P2Y(1) receptors to ADP signalling in mouse spinal cord cultures |
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