Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects
To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combina...
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| Veröffentlicht in: | Redox report : communications in free radical research 2008-02, Vol.13 (1), p.17-22 |
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| creator | Adžić, Miroslav Nićiforović, Ana Zarić, Božidarka Nešković-Konstantinović, Zora Spasić, Snežana D. Jones, David R. Radojčić, Marija B. |
| description | To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4
+
lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4
+
cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4
+
cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour. |
| doi_str_mv | 10.1179/135100008X259088 |
| format | Article |
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+
lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4
+
cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4
+
cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.</description><identifier>ISSN: 1351-0002</identifier><identifier>EISSN: 1743-2928</identifier><identifier>DOI: 10.1179/135100008X259088</identifier><identifier>PMID: 18284847</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>BREAST CANCER ; Breast Neoplasms - pathology ; CATALASE ; Catalase - metabolism ; Catalase - radiation effects ; Cell Culture Techniques - methods ; Female ; Glutathione Peroxidase - metabolism ; Glutathione Peroxidase - radiation effects ; Humans ; IONISING RADIATION ; LEUKOCYTES ; Leukocytes - radiation effects ; NF-kappa B - metabolism ; NF-kappa B - radiation effects ; Oxidative Stress ; Radiation, Ionizing ; Reference Values ; SUPEROXIDE DISMUTASE ; Superoxide Dismutase - metabolism ; Superoxide Dismutase - radiation effects</subject><ispartof>Redox report : communications in free radical research, 2008-02, Vol.13 (1), p.17-22</ispartof><rights>2008 Maney Publishing 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c344t-af48441a1ec5a78be8ec98f1fc8b922e3f1ab3eca63a62955368454c583600713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18284847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adžić, Miroslav</creatorcontrib><creatorcontrib>Nićiforović, Ana</creatorcontrib><creatorcontrib>Zarić, Božidarka</creatorcontrib><creatorcontrib>Nešković-Konstantinović, Zora</creatorcontrib><creatorcontrib>Spasić, Snežana D.</creatorcontrib><creatorcontrib>Jones, David R.</creatorcontrib><creatorcontrib>Radojčić, Marija B.</creatorcontrib><title>Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects</title><title>Redox report : communications in free radical research</title><addtitle>Redox Rep</addtitle><description>To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4
+
lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4
+
cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4
+
cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.</description><subject>BREAST CANCER</subject><subject>Breast Neoplasms - pathology</subject><subject>CATALASE</subject><subject>Catalase - metabolism</subject><subject>Catalase - radiation effects</subject><subject>Cell Culture Techniques - methods</subject><subject>Female</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Glutathione Peroxidase - radiation effects</subject><subject>Humans</subject><subject>IONISING RADIATION</subject><subject>LEUKOCYTES</subject><subject>Leukocytes - radiation effects</subject><subject>NF-kappa B - metabolism</subject><subject>NF-kappa B - radiation effects</subject><subject>Oxidative Stress</subject><subject>Radiation, Ionizing</subject><subject>Reference Values</subject><subject>SUPEROXIDE DISMUTASE</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Superoxide Dismutase - radiation effects</subject><issn>1351-0002</issn><issn>1743-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUuLFDEQgBtR3HX17knq5K016fQjjScZfMGCFwVvTTpdcbKmkzaVRuZH-R-tZQYEwVxSob76ilRV1XMpXkk5jK-l6qTgo7813Si0flBdy6FVdTM2-iHHnK453VxVT4juOFL9qB9XV1I3utXtcF39PmAIYPdQ9oxgU1x88SkSbKlgLN4UhMU7hxmjRQIfoRwRMtLGFEJJwLgnH79DNgvz_ITkYMPstyNmE2AOKS0QcP-R7KncSygFFi_gclphzmiogDXcIMPGBm5MYOICRzShHE9A-3yHttDT6pEzgfDZ5b6pvr5_9-Xwsb79_OHT4e1tbVXblto4_lwrjUTbmUHPqNGO2kln9Tw2DSonzazQml6Zvhm7TvW67VrbadULMUh1U708e7ecfu5IZVo9WZ6UiZh2mgah5CikYlCcQZsTUUY3bdmvJp8mKab7FU3_rohLXlzc-7zi8rfgshMG3pwBH13Kq_mVclimYk4hZZd5Sp4m9V_9H5Oio94</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Adžić, Miroslav</creator><creator>Nićiforović, Ana</creator><creator>Zarić, Božidarka</creator><creator>Nešković-Konstantinović, Zora</creator><creator>Spasić, Snežana D.</creator><creator>Jones, David R.</creator><creator>Radojčić, Marija B.</creator><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080201</creationdate><title>Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects</title><author>Adžić, Miroslav ; Nićiforović, Ana ; Zarić, Božidarka ; Nešković-Konstantinović, Zora ; Spasić, Snežana D. ; Jones, David R. ; Radojčić, Marija B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-af48441a1ec5a78be8ec98f1fc8b922e3f1ab3eca63a62955368454c583600713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>BREAST CANCER</topic><topic>Breast Neoplasms - pathology</topic><topic>CATALASE</topic><topic>Catalase - metabolism</topic><topic>Catalase - radiation effects</topic><topic>Cell Culture Techniques - methods</topic><topic>Female</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Glutathione Peroxidase - radiation effects</topic><topic>Humans</topic><topic>IONISING RADIATION</topic><topic>LEUKOCYTES</topic><topic>Leukocytes - radiation effects</topic><topic>NF-kappa B - metabolism</topic><topic>NF-kappa B - radiation effects</topic><topic>Oxidative Stress</topic><topic>Radiation, Ionizing</topic><topic>Reference Values</topic><topic>SUPEROXIDE DISMUTASE</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Superoxide Dismutase - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adžić, Miroslav</creatorcontrib><creatorcontrib>Nićiforović, Ana</creatorcontrib><creatorcontrib>Zarić, Božidarka</creatorcontrib><creatorcontrib>Nešković-Konstantinović, Zora</creatorcontrib><creatorcontrib>Spasić, Snežana D.</creatorcontrib><creatorcontrib>Jones, David R.</creatorcontrib><creatorcontrib>Radojčić, Marija B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Redox report : communications in free radical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adžić, Miroslav</au><au>Nićiforović, Ana</au><au>Zarić, Božidarka</au><au>Nešković-Konstantinović, Zora</au><au>Spasić, Snežana D.</au><au>Jones, David R.</au><au>Radojčić, Marija B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects</atitle><jtitle>Redox report : communications in free radical research</jtitle><addtitle>Redox Rep</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>13</volume><issue>1</issue><spage>17</spage><epage>22</epage><pages>17-22</pages><issn>1351-0002</issn><eissn>1743-2928</eissn><abstract>To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-κB and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4
+
lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P < 0.001) and after (P < 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P < 0.001] and 9 Gy [P < 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4
+
cells was significantly (P < 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4
+
cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>18284847</pmid><doi>10.1179/135100008X259088</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Redox report : communications in free radical research, 2008-02, Vol.13 (1), p.17-22 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | BREAST CANCER Breast Neoplasms - pathology CATALASE Catalase - metabolism Catalase - radiation effects Cell Culture Techniques - methods Female Glutathione Peroxidase - metabolism Glutathione Peroxidase - radiation effects Humans IONISING RADIATION LEUKOCYTES Leukocytes - radiation effects NF-kappa B - metabolism NF-kappa B - radiation effects Oxidative Stress Radiation, Ionizing Reference Values SUPEROXIDE DISMUTASE Superoxide Dismutase - metabolism Superoxide Dismutase - radiation effects |
| title | Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects |
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