Epitope mapping of antibodies against S-tagged fusion proteins and molecular weight markers

Monoclonal antibodies against S-tagged fusion proteins expressed in pET vectors were generated and further characterized. Most pET vectors contain a 15meric S-tag as a fusion tag for the detection of recombinant proteins. Two antibodies, G18BA3 and G18BE8, recognized this S-tag in enzyme immunoassay...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2008-02, Vol.72 (2), p.346-351
Hauptverfasser:	Daskalow, K.(University of Potsdam, Golm (Germany)), Boisguerin, P, Jandrig, B, Volkmer, R, Micheel, B, Schenk, J.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence AMINO ACID SEQUENCES Animals Antibodies - chemistry Antibodies - immunology ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES Biological and medical sciences Blotting, Western Electrophoresis, Polyacrylamide Gel ENZIMAS ENZYME ENZYMES Epitope Mapping Fundamental and applied biological sciences. Psychology GENETIC TRANSFORMATION hybridoma IMMUNOENZYME TECHNIQUES Mice Mice, Inbred BALB C Molecular Sequence Data MOLECULAR WEIGHT molecular weight standard MONOCLONAL ANTIBODIES monoclonal antibody PEPTIDE PEPTIDES PEPTIDOS PESO MOLECULAR POIDS MOLECULAIRE PROTEINAS PROTEINAS RECOMBINANTES PROTEINE PROTEINE RECOMBINANTE PROTEINS Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - immunology RECOMBINANT PROTEINS SECUENCIAS DE AMINOACIDOS SEQUENCE D'ACIDES AMINES substitution analysis TECHNIQUE IMMUNOENZYMATIQUE TECNICAS INMUNOENZIMATICAS TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE
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