Effects of Methotrexate on the Tubal Morphology of Rabbits: Evaluation by Electron Microscopy
Background/Aims: To evaluate by electron microscopy the effects of methotrexate on the tubal morphology of rabbits. Methods: Biopsies were taken from the isthmic portion of both tubes of 4 rabbits in the control group. 1 mg/kg methotrexate was injected in the left tubes and as a control, a 14-gauge...
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| Veröffentlicht in: | Gynecologic and obstetric investigation 2008-01, Vol.65 (4), p.217-221 |
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| container_title | Gynecologic and obstetric investigation |
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| creator | Çetin, M. Turan Arısoy, A. Hakim Tap, Özgül Kaya, Mehmet Ürünsak, İbrahim |
| description | Background/Aims: To evaluate by electron microscopy the effects of methotrexate on the tubal morphology of rabbits. Methods: Biopsies were taken from the isthmic portion of both tubes of 4 rabbits in the control group. 1 mg/kg methotrexate was injected in the left tubes and as a control, a 14-gauge fine needle was used to puncture the right tubes of 10 rabbits in the study group. One month later, tubal biopsies were taken from both tubes of every rabbit in the study group. To mimic pregnancy, 100 IU hCG was injected intramuscularly to every rabbit 24 h before every surgery. On examination by electron microscopy, the effects of methotrexate and of the damage with the fine needle on the tubes were compared to the control group. Results: Young epithelial cells of the methotrexate-injected fallopian tubes kept their normal structure, but others contained important ultrastructural changes. These were: nuclear pyknosis, cytoplasmic vacuolization, dilatation of endoplasmic reticulum cisternae, increased cytoplasmic density and compound cilia. Conclusion: Degenerative changes on epithelial cells caused by the temporary blockage of mitotic activity associated with local methotrexate injection are reversible through the formation of new epithelial cells. |
| doi_str_mv | 10.1159/000113043 |
| format | Article |
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Turan ; Arısoy, A. Hakim ; Tap, Özgül ; Kaya, Mehmet ; Ürünsak, İbrahim</creator><creatorcontrib>Çetin, M. Turan ; Arısoy, A. Hakim ; Tap, Özgül ; Kaya, Mehmet ; Ürünsak, İbrahim</creatorcontrib><description>Background/Aims: To evaluate by electron microscopy the effects of methotrexate on the tubal morphology of rabbits. Methods: Biopsies were taken from the isthmic portion of both tubes of 4 rabbits in the control group. 1 mg/kg methotrexate was injected in the left tubes and as a control, a 14-gauge fine needle was used to puncture the right tubes of 10 rabbits in the study group. One month later, tubal biopsies were taken from both tubes of every rabbit in the study group. To mimic pregnancy, 100 IU hCG was injected intramuscularly to every rabbit 24 h before every surgery. On examination by electron microscopy, the effects of methotrexate and of the damage with the fine needle on the tubes were compared to the control group. Results: Young epithelial cells of the methotrexate-injected fallopian tubes kept their normal structure, but others contained important ultrastructural changes. These were: nuclear pyknosis, cytoplasmic vacuolization, dilatation of endoplasmic reticulum cisternae, increased cytoplasmic density and compound cilia. Conclusion: Degenerative changes on epithelial cells caused by the temporary blockage of mitotic activity associated with local methotrexate injection are reversible through the formation of new epithelial cells.</description><identifier>ISSN: 0378-7346</identifier><identifier>EISSN: 1423-002X</identifier><identifier>DOI: 10.1159/000113043</identifier><identifier>PMID: 18196902</identifier><identifier>CODEN: GOBIDS</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Abortifacient Agents, Nonsteroidal - pharmacology ; Animals ; Biological and medical sciences ; Epithelium - drug effects ; Epithelium - ultrastructure ; Fallopian Tubes - drug effects ; Fallopian Tubes - pathology ; Fallopian Tubes - ultrastructure ; Female ; Gynecology. Andrology. Obstetrics ; Medical sciences ; Methotrexate - pharmacology ; Microscopy, Electron ; Mitosis - drug effects ; Original Article ; Pregnancy ; Rabbits</subject><ispartof>Gynecologic and obstetric investigation, 2008-01, Vol.65 (4), p.217-221</ispartof><rights>2008 S. Karger AG, Basel</rights><rights>2008 INIST-CNRS</rights><rights>(c) 2008 S. Karger AG, Basel</rights><rights>Copyright (c) 2008 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-316d3798c02f772a2ab70802e2df77daecb64ba45dbc34868a8f4dd580f4080a3</citedby><cites>FETCH-LOGICAL-c361t-316d3798c02f772a2ab70802e2df77daecb64ba45dbc34868a8f4dd580f4080a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20389897$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18196902$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Çetin, M. Turan</creatorcontrib><creatorcontrib>Arısoy, A. Hakim</creatorcontrib><creatorcontrib>Tap, Özgül</creatorcontrib><creatorcontrib>Kaya, Mehmet</creatorcontrib><creatorcontrib>Ürünsak, İbrahim</creatorcontrib><title>Effects of Methotrexate on the Tubal Morphology of Rabbits: Evaluation by Electron Microscopy</title><title>Gynecologic and obstetric investigation</title><addtitle>Gynecol Obstet Invest</addtitle><description>Background/Aims: To evaluate by electron microscopy the effects of methotrexate on the tubal morphology of rabbits. Methods: Biopsies were taken from the isthmic portion of both tubes of 4 rabbits in the control group. 1 mg/kg methotrexate was injected in the left tubes and as a control, a 14-gauge fine needle was used to puncture the right tubes of 10 rabbits in the study group. One month later, tubal biopsies were taken from both tubes of every rabbit in the study group. To mimic pregnancy, 100 IU hCG was injected intramuscularly to every rabbit 24 h before every surgery. On examination by electron microscopy, the effects of methotrexate and of the damage with the fine needle on the tubes were compared to the control group. Results: Young epithelial cells of the methotrexate-injected fallopian tubes kept their normal structure, but others contained important ultrastructural changes. These were: nuclear pyknosis, cytoplasmic vacuolization, dilatation of endoplasmic reticulum cisternae, increased cytoplasmic density and compound cilia. Conclusion: Degenerative changes on epithelial cells caused by the temporary blockage of mitotic activity associated with local methotrexate injection are reversible through the formation of new epithelial cells.</description><subject>Abortifacient Agents, Nonsteroidal - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Epithelium - drug effects</subject><subject>Epithelium - ultrastructure</subject><subject>Fallopian Tubes - drug effects</subject><subject>Fallopian Tubes - pathology</subject><subject>Fallopian Tubes - ultrastructure</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Medical sciences</subject><subject>Methotrexate - pharmacology</subject><subject>Microscopy, Electron</subject><subject>Mitosis - drug effects</subject><subject>Original Article</subject><subject>Pregnancy</subject><subject>Rabbits</subject><issn>0378-7346</issn><issn>1423-002X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0M9LHDEUB_BQKnVrPXgvZSi04GHalx-bZHoTWWvBpVAseJHhJZO4Y2c3a5IR979vll0UPCWBD9_38iXkhMI3SqfNdwCglIPgb8iECsZrAHbzlkyAK10rLuQheZ_SfVHTRot35JBq2sgG2ITczrx3Nqcq-Gru8iLk6J4wuyqsqrxw1fVocKjmIa4XYQh3m637g8b0Of2oZo84jJj7Ys2mmg0lKJb7vLcxJBvWmw_kwOOQ3PH-PCJ_L2bX55f11e-fv87PrmrLJc01p7LjqtEWmFeKIUOjQANzrCvvDp01UhgU085YLrTUqL3ouqkGL4pDfkS-7nLXMTyMLuV22SfrhgFXLoypVVRyLTQv8PMreB_GuCq7tYxxJUFCU9DpDm2_kaLz7Tr2S4yblkK7Lbx9LrzYT_vA0Sxd9yL3DRfwZQ8wWRx8xJXt07NjwHWjG1Xcx537h_HOxZeg3Zz_kFqQAQ</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Çetin, M. 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Hakim ; Tap, Özgül ; Kaya, Mehmet ; Ürünsak, İbrahim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-316d3798c02f772a2ab70802e2df77daecb64ba45dbc34868a8f4dd580f4080a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Abortifacient Agents, Nonsteroidal - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - ultrastructure</topic><topic>Fallopian Tubes - drug effects</topic><topic>Fallopian Tubes - pathology</topic><topic>Fallopian Tubes - ultrastructure</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Medical sciences</topic><topic>Methotrexate - pharmacology</topic><topic>Microscopy, Electron</topic><topic>Mitosis - drug effects</topic><topic>Original Article</topic><topic>Pregnancy</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Çetin, M. Turan</creatorcontrib><creatorcontrib>Arısoy, A. 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Turan</au><au>Arısoy, A. Hakim</au><au>Tap, Özgül</au><au>Kaya, Mehmet</au><au>Ürünsak, İbrahim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Methotrexate on the Tubal Morphology of Rabbits: Evaluation by Electron Microscopy</atitle><jtitle>Gynecologic and obstetric investigation</jtitle><addtitle>Gynecol Obstet Invest</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>65</volume><issue>4</issue><spage>217</spage><epage>221</epage><pages>217-221</pages><issn>0378-7346</issn><eissn>1423-002X</eissn><coden>GOBIDS</coden><abstract>Background/Aims: To evaluate by electron microscopy the effects of methotrexate on the tubal morphology of rabbits. Methods: Biopsies were taken from the isthmic portion of both tubes of 4 rabbits in the control group. 1 mg/kg methotrexate was injected in the left tubes and as a control, a 14-gauge fine needle was used to puncture the right tubes of 10 rabbits in the study group. One month later, tubal biopsies were taken from both tubes of every rabbit in the study group. To mimic pregnancy, 100 IU hCG was injected intramuscularly to every rabbit 24 h before every surgery. On examination by electron microscopy, the effects of methotrexate and of the damage with the fine needle on the tubes were compared to the control group. Results: Young epithelial cells of the methotrexate-injected fallopian tubes kept their normal structure, but others contained important ultrastructural changes. These were: nuclear pyknosis, cytoplasmic vacuolization, dilatation of endoplasmic reticulum cisternae, increased cytoplasmic density and compound cilia. Conclusion: Degenerative changes on epithelial cells caused by the temporary blockage of mitotic activity associated with local methotrexate injection are reversible through the formation of new epithelial cells.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>18196902</pmid><doi>10.1159/000113043</doi><tpages>5</tpages></addata></record> |
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| ispartof | Gynecologic and obstetric investigation, 2008-01, Vol.65 (4), p.217-221 |
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| source | Karger Journals; MEDLINE |
| subjects | Abortifacient Agents, Nonsteroidal - pharmacology Animals Biological and medical sciences Epithelium - drug effects Epithelium - ultrastructure Fallopian Tubes - drug effects Fallopian Tubes - pathology Fallopian Tubes - ultrastructure Female Gynecology. Andrology. Obstetrics Medical sciences Methotrexate - pharmacology Microscopy, Electron Mitosis - drug effects Original Article Pregnancy Rabbits |
| title | Effects of Methotrexate on the Tubal Morphology of Rabbits: Evaluation by Electron Microscopy |
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