Orally administered lactoperoxidase increases expression of the FK506 binding protein 5 gene in epithelial cells of the small intestine of mice: A DNA microarray study
Lactoperoxidase (LPO) is a component of milk and other external secretions. To study the influence of ingested LPO on the digestive tract, we performed DNA microarray analysis of the small intestine of mice administered LPO. LPO administration upregulated 78 genes, including genes involved in metabo...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2007-09, Vol.71 (9), p.2274-2282 |
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| creator | Wakabayashi, H.(Morinaga Milk Industry Co. Ltd., Zama, Kanagawa (Japan). Food Science and Technology Inst.) Miyauchi, H Shin, K Yamauchi, K Matsumoto, I Abe, K Takase, M |
| description | Lactoperoxidase (LPO) is a component of milk and other external secretions. To study the influence of ingested LPO on the digestive tract, we performed DNA microarray analysis of the small intestine of mice administered LPO. LPO administration upregulated 78 genes, including genes involved in metabolism, immunity, apoptosis, and the cell cycle, and downregulated nine genes, including immunity-related genes. The most upregulated gene was FK506 binding protein 5 (FKBP5), a glucocorticoid regulating immunophilin. The upregulation of this gene was confirmed by quantitative RT-PCR in other samples. In situ hybridization revealed that expression of the FKBP5 gene in the crypt epithelial cells of the small intestine was enhanced by LPO. These results suggest that ingested LPO modulates gene expression in the small intestine and especially increases FKBP5 gene expression in the epithelial cells of the intestine. |
| doi_str_mv | 10.1271/bbb.70255 |
| format | Article |
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Ltd., Zama, Kanagawa (Japan). Food Science and Technology Inst.) ; Miyauchi, H ; Shin, K ; Yamauchi, K ; Matsumoto, I ; Abe, K ; Takase, M</creator><creatorcontrib>Wakabayashi, H.(Morinaga Milk Industry Co. Ltd., Zama, Kanagawa (Japan). Food Science and Technology Inst.) ; Miyauchi, H ; Shin, K ; Yamauchi, K ; Matsumoto, I ; Abe, K ; Takase, M</creatorcontrib><description>Lactoperoxidase (LPO) is a component of milk and other external secretions. To study the influence of ingested LPO on the digestive tract, we performed DNA microarray analysis of the small intestine of mice administered LPO. LPO administration upregulated 78 genes, including genes involved in metabolism, immunity, apoptosis, and the cell cycle, and downregulated nine genes, including immunity-related genes. The most upregulated gene was FK506 binding protein 5 (FKBP5), a glucocorticoid regulating immunophilin. The upregulation of this gene was confirmed by quantitative RT-PCR in other samples. In situ hybridization revealed that expression of the FKBP5 gene in the crypt epithelial cells of the small intestine was enhanced by LPO. These results suggest that ingested LPO modulates gene expression in the small intestine and especially increases FKBP5 gene expression in the epithelial cells of the intestine.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.70255</identifier><identifier>PMID: 17827675</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Administration, Oral ; ADN ; Animals ; Biological and medical sciences ; Cattle ; Cell Line ; DNA ; DNA microarray ; Down-Regulation ; EPITELIO ; epithelial cells ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; EPITHELIUM ; EXPRESION GENICA ; EXPRESSION DES GENES ; Female ; FK506 binding protein 5 ; Fundamental and applied biological sciences. Psychology ; GENE EXPRESSION ; INTESTIN ; Intestine, Small - drug effects ; Intestine, Small - metabolism ; INTESTINES ; INTESTINOS ; LACTOPEROXIDASA ; LACTOPEROXIDASE ; Lactoperoxidase - administration & dosage ; Lactoperoxidase - pharmacology ; LACTOPEROXYDASE ; MICE ; NUCLEOTIDE SEQUENCE ; Oligonucleotide Array Sequence Analysis ; RATON ; RNA, Messenger - genetics ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; small intestine ; SOURIS ; Tacrolimus Binding Proteins - genetics ; Tacrolimus Binding Proteins - metabolism ; Up-Regulation</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2007-09, Vol.71 (9), p.2274-2282</ispartof><rights>2007 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2007</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c662t-42a4e34f8cc290696d1e8fef922bdfec2502a97b5203b83019246632a59198553</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19203403$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17827675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wakabayashi, H.(Morinaga Milk Industry Co. Ltd., Zama, Kanagawa (Japan). Food Science and Technology Inst.)</creatorcontrib><creatorcontrib>Miyauchi, H</creatorcontrib><creatorcontrib>Shin, K</creatorcontrib><creatorcontrib>Yamauchi, K</creatorcontrib><creatorcontrib>Matsumoto, I</creatorcontrib><creatorcontrib>Abe, K</creatorcontrib><creatorcontrib>Takase, M</creatorcontrib><title>Orally administered lactoperoxidase increases expression of the FK506 binding protein 5 gene in epithelial cells of the small intestine of mice: A DNA microarray study</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Lactoperoxidase (LPO) is a component of milk and other external secretions. To study the influence of ingested LPO on the digestive tract, we performed DNA microarray analysis of the small intestine of mice administered LPO. LPO administration upregulated 78 genes, including genes involved in metabolism, immunity, apoptosis, and the cell cycle, and downregulated nine genes, including immunity-related genes. The most upregulated gene was FK506 binding protein 5 (FKBP5), a glucocorticoid regulating immunophilin. The upregulation of this gene was confirmed by quantitative RT-PCR in other samples. In situ hybridization revealed that expression of the FKBP5 gene in the crypt epithelial cells of the small intestine was enhanced by LPO. These results suggest that ingested LPO modulates gene expression in the small intestine and especially increases FKBP5 gene expression in the epithelial cells of the intestine.</description><subject>Administration, Oral</subject><subject>ADN</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cell Line</subject><subject>DNA</subject><subject>DNA microarray</subject><subject>Down-Regulation</subject><subject>EPITELIO</subject><subject>epithelial cells</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>EPITHELIUM</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Female</subject><subject>FK506 binding protein 5</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE EXPRESSION</subject><subject>INTESTIN</subject><subject>Intestine, Small - drug effects</subject><subject>Intestine, Small - metabolism</subject><subject>INTESTINES</subject><subject>INTESTINOS</subject><subject>LACTOPEROXIDASA</subject><subject>LACTOPEROXIDASE</subject><subject>Lactoperoxidase - administration & dosage</subject><subject>Lactoperoxidase - pharmacology</subject><subject>LACTOPEROXYDASE</subject><subject>MICE</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>RATON</subject><subject>RNA, Messenger - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>small intestine</subject><subject>SOURIS</subject><subject>Tacrolimus Binding Proteins - genetics</subject><subject>Tacrolimus Binding Proteins - metabolism</subject><subject>Up-Regulation</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctu1DAUAFALgehQWPABIG9AYjHF78TsRoXyqigLWEeOcz0YOXZqZ0TzRfwmHmaqbpBY-co696WL0FNKzihr6Ou-788awqS8h1aUi2attGjuoxXRVK1bIekJelTKT0Lqh6QP0QltWtaoRq7Q76tsQliwGUYffZkhw4CDsXOaIKcbP5gC2EeboQYFw82UoRSfIk4Ozz8AX3yWROHex8HHLZ5ymsFHLPEW4j4Rw-QrC94EbCGEcptXxtq3ghnK7Cut36O38AZv8Nsvm32ck8nZLLjMu2F5jB44Ewo8Ob6n6PvFu2_nH9aXV-8_nm8u11YpNq8FMwK4cK21TBOl1UChdeA0Y_3gwDJJmNFNLxnhfcsJ1UwoxZmRmupWSn6KXh7q1k2ud3W2bvRlP7iJkHalUy2n1ev_QqobQRQnFb46wLpQKRlcN2U_mrx0lHT783X1fN3f81X7_Fh0148w3MnjvSp4cQSmWBNcNtH6cud03UsQXp04OB9dyqP5lXIYutksIeXbJP6v_s8Oac6kzmxzVZ--MkJaQihnnP8BYYi96A</recordid><startdate>20070901</startdate><enddate>20070901</enddate><creator>Wakabayashi, H.(Morinaga Milk Industry Co. 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Food Science and Technology Inst.)</creatorcontrib><creatorcontrib>Miyauchi, H</creatorcontrib><creatorcontrib>Shin, K</creatorcontrib><creatorcontrib>Yamauchi, K</creatorcontrib><creatorcontrib>Matsumoto, I</creatorcontrib><creatorcontrib>Abe, K</creatorcontrib><creatorcontrib>Takase, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wakabayashi, H.(Morinaga Milk Industry Co. Ltd., Zama, Kanagawa (Japan). Food Science and Technology Inst.)</au><au>Miyauchi, H</au><au>Shin, K</au><au>Yamauchi, K</au><au>Matsumoto, I</au><au>Abe, K</au><au>Takase, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Orally administered lactoperoxidase increases expression of the FK506 binding protein 5 gene in epithelial cells of the small intestine of mice: A DNA microarray study</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2007-09-01</date><risdate>2007</risdate><volume>71</volume><issue>9</issue><spage>2274</spage><epage>2282</epage><pages>2274-2282</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Lactoperoxidase (LPO) is a component of milk and other external secretions. To study the influence of ingested LPO on the digestive tract, we performed DNA microarray analysis of the small intestine of mice administered LPO. LPO administration upregulated 78 genes, including genes involved in metabolism, immunity, apoptosis, and the cell cycle, and downregulated nine genes, including immunity-related genes. The most upregulated gene was FK506 binding protein 5 (FKBP5), a glucocorticoid regulating immunophilin. The upregulation of this gene was confirmed by quantitative RT-PCR in other samples. In situ hybridization revealed that expression of the FKBP5 gene in the crypt epithelial cells of the small intestine was enhanced by LPO. These results suggest that ingested LPO modulates gene expression in the small intestine and especially increases FKBP5 gene expression in the epithelial cells of the intestine.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>17827675</pmid><doi>10.1271/bbb.70255</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; Free Full-Text Journals in Chemistry |
| subjects | Administration, Oral ADN Animals Biological and medical sciences Cattle Cell Line DNA DNA microarray Down-Regulation EPITELIO epithelial cells Epithelial Cells - drug effects Epithelial Cells - metabolism EPITHELIUM EXPRESION GENICA EXPRESSION DES GENES Female FK506 binding protein 5 Fundamental and applied biological sciences. Psychology GENE EXPRESSION INTESTIN Intestine, Small - drug effects Intestine, Small - metabolism INTESTINES INTESTINOS LACTOPEROXIDASA LACTOPEROXIDASE Lactoperoxidase - administration & dosage Lactoperoxidase - pharmacology LACTOPEROXYDASE MICE NUCLEOTIDE SEQUENCE Oligonucleotide Array Sequence Analysis RATON RNA, Messenger - genetics SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE small intestine SOURIS Tacrolimus Binding Proteins - genetics Tacrolimus Binding Proteins - metabolism Up-Regulation |
| title | Orally administered lactoperoxidase increases expression of the FK506 binding protein 5 gene in epithelial cells of the small intestine of mice: A DNA microarray study |
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