The effect of ionizing irradiation on the oxidation of palmitate-1-14C by thymus and liver
New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours followin...
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| Veröffentlicht in: | Radiation research 1968-11, Vol.36 (2), p.208 |
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| creator | Miras, C J Mantzos, J D Legakis, N J Levis, G M |
| description | New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxidation by liver preparations was not significantly affected. The rate of oxidation by thymus was decreased to less than one-third of the control value within 12 hours from irradiation and, at 24 hours, was almost completely abolished. Increased ATP concentration could increase only to a small extent the oxidation by thymus preparations of irradiated animals. Oxidation by isolated thymus mitochondria was also inhibited by irradiation. Counting of the water-soluble oxidation products of palmitate-1-14C suggests that the inhibition is not due to the impairment of the reactions of the citric acid cycle. The non-esterified fatty acid concentration of thymus was not altered at 12 hours following irradiation. Esterification of K+-palmitate-l-14C into the thymus lipids was not affected 12 hours after irradiation. |
| doi_str_mv | 10.2307/3572646 |
| format | Article |
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K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxidation by liver preparations was not significantly affected. The rate of oxidation by thymus was decreased to less than one-third of the control value within 12 hours from irradiation and, at 24 hours, was almost completely abolished. Increased ATP concentration could increase only to a small extent the oxidation by thymus preparations of irradiated animals. Oxidation by isolated thymus mitochondria was also inhibited by irradiation. Counting of the water-soluble oxidation products of palmitate-1-14C suggests that the inhibition is not due to the impairment of the reactions of the citric acid cycle. The non-esterified fatty acid concentration of thymus was not altered at 12 hours following irradiation. Esterification of K+-palmitate-l-14C into the thymus lipids was not affected 12 hours after irradiation.</description><identifier>ISSN: 0033-7587</identifier><identifier>DOI: 10.2307/3572646</identifier><identifier>PMID: 17387940</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Carbon Radioisotopes - metabolism ; Cells, Cultured ; Dose-Response Relationship, Radiation ; Female ; Liver - metabolism ; Liver - radiation effects ; Male ; Mitochondria - metabolism ; Mitochondria - radiation effects ; Oxidation-Reduction - radiation effects ; Palmitates - metabolism ; Rabbits ; Radiation Dosage ; Thymus Gland - metabolism ; Thymus Gland - radiation effects ; Whole-Body Irradiation</subject><ispartof>Radiation research, 1968-11, Vol.36 (2), p.208</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17387940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miras, C J</creatorcontrib><creatorcontrib>Mantzos, J D</creatorcontrib><creatorcontrib>Legakis, N J</creatorcontrib><creatorcontrib>Levis, G M</creatorcontrib><title>The effect of ionizing irradiation on the oxidation of palmitate-1-14C by thymus and liver</title><title>Radiation research</title><addtitle>Radiat Res</addtitle><description>New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxidation by liver preparations was not significantly affected. The rate of oxidation by thymus was decreased to less than one-third of the control value within 12 hours from irradiation and, at 24 hours, was almost completely abolished. Increased ATP concentration could increase only to a small extent the oxidation by thymus preparations of irradiated animals. Oxidation by isolated thymus mitochondria was also inhibited by irradiation. Counting of the water-soluble oxidation products of palmitate-1-14C suggests that the inhibition is not due to the impairment of the reactions of the citric acid cycle. The non-esterified fatty acid concentration of thymus was not altered at 12 hours following irradiation. Esterification of K+-palmitate-l-14C into the thymus lipids was not affected 12 hours after irradiation.</description><subject>Animals</subject><subject>Carbon Radioisotopes - metabolism</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Female</subject><subject>Liver - metabolism</subject><subject>Liver - radiation effects</subject><subject>Male</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - radiation effects</subject><subject>Oxidation-Reduction - radiation effects</subject><subject>Palmitates - metabolism</subject><subject>Rabbits</subject><subject>Radiation Dosage</subject><subject>Thymus Gland - metabolism</subject><subject>Thymus Gland - radiation effects</subject><subject>Whole-Body Irradiation</subject><issn>0033-7587</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1j8lKBDEYhHNQnHEU30DyAtE_e3KUxg0GvIwXL0PSSTTSG90ZsX16GxyhoKiPoqAQuqJwwzjoWy41U0KdoDUA50RLo1fofJo-YclU2TO0opobbQWs0dvuI-KYUqwL7hPOfZd_cveO8zi6kF1ZAF5Ullb_ncMRJDy4ps3FlUgooaLCfl46c3uYsOsCbvJXHC_QaXLNFC-PvkGvD_e76olsXx6fq7stGRjYQoz0tRVGKwBljHXSGM4lM44p6oWFJEUtwGtvtaVUgXGUgZRG6CCUToFv0PXf7nDwbQz7YcytG-f9_0n-C0dUTfU</recordid><startdate>196811</startdate><enddate>196811</enddate><creator>Miras, C J</creator><creator>Mantzos, J D</creator><creator>Legakis, N J</creator><creator>Levis, G M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>196811</creationdate><title>The effect of ionizing irradiation on the oxidation of palmitate-1-14C by thymus and liver</title><author>Miras, C J ; Mantzos, J D ; Legakis, N J ; Levis, G M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p209t-85bc94876006889a58833528a261b490f54c40b7b97911608a12055847d467fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Animals</topic><topic>Carbon Radioisotopes - metabolism</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Female</topic><topic>Liver - metabolism</topic><topic>Liver - radiation effects</topic><topic>Male</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria - radiation effects</topic><topic>Oxidation-Reduction - radiation effects</topic><topic>Palmitates - metabolism</topic><topic>Rabbits</topic><topic>Radiation Dosage</topic><topic>Thymus Gland - metabolism</topic><topic>Thymus Gland - radiation effects</topic><topic>Whole-Body Irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miras, C J</creatorcontrib><creatorcontrib>Mantzos, J D</creatorcontrib><creatorcontrib>Legakis, N J</creatorcontrib><creatorcontrib>Levis, G M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Radiation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miras, C J</au><au>Mantzos, J D</au><au>Legakis, N J</au><au>Levis, G M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of ionizing irradiation on the oxidation of palmitate-1-14C by thymus and liver</atitle><jtitle>Radiation research</jtitle><addtitle>Radiat Res</addtitle><date>1968-11</date><risdate>1968</risdate><volume>36</volume><issue>2</issue><spage>208</spage><pages>208-</pages><issn>0033-7587</issn><abstract>New Zealand rabbits, fasted for 12 hours, were subjected to 500 rads of whole-body irradiation. K+-palmitate-1-14C oxidation was assayed with the 600 X g supernatant of thymus and liver homogenates, in the presence of ATP, at various time intervals from irradiation. For a period of 24 hours following irradiation, oxidation by liver preparations was not significantly affected. The rate of oxidation by thymus was decreased to less than one-third of the control value within 12 hours from irradiation and, at 24 hours, was almost completely abolished. Increased ATP concentration could increase only to a small extent the oxidation by thymus preparations of irradiated animals. Oxidation by isolated thymus mitochondria was also inhibited by irradiation. Counting of the water-soluble oxidation products of palmitate-1-14C suggests that the inhibition is not due to the impairment of the reactions of the citric acid cycle. The non-esterified fatty acid concentration of thymus was not altered at 12 hours following irradiation. Esterification of K+-palmitate-l-14C into the thymus lipids was not affected 12 hours after irradiation.</abstract><cop>United States</cop><pmid>17387940</pmid><doi>10.2307/3572646</doi></addata></record> |
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| ispartof | Radiation research, 1968-11, Vol.36 (2), p.208 |
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| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing |
| subjects | Animals Carbon Radioisotopes - metabolism Cells, Cultured Dose-Response Relationship, Radiation Female Liver - metabolism Liver - radiation effects Male Mitochondria - metabolism Mitochondria - radiation effects Oxidation-Reduction - radiation effects Palmitates - metabolism Rabbits Radiation Dosage Thymus Gland - metabolism Thymus Gland - radiation effects Whole-Body Irradiation |
| title | The effect of ionizing irradiation on the oxidation of palmitate-1-14C by thymus and liver |
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