HrpN(ea) Harpin from Erwinia amylovora Triggers Differential Responses on the Nonhost Arabidopsis thaliana Cells and on the Host Apple Cells
Erwinia amylovora is a gram-negative necrogenic bacterium causing fire blight of the Maloideae subfamily of Rosaceae such as apple and pear. It provokes progressive necrosis in aerial parts of susceptible host plants (compatible interaction) and a hypersensitive reaction (HR) when infiltrated in non...
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| Veröffentlicht in: | Molecular plant-microbe interactions 2007, Vol.20 (1), p.94-100 |
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| creator | Reboutier, D Frankart, C Briand, J Biligui, B Laroche, S Rona, J.P Barny, M.A Bouteau, F |
| description | Erwinia amylovora is a gram-negative necrogenic bacterium causing fire blight of the Maloideae subfamily of Rosaceae such as apple and pear. It provokes progressive necrosis in aerial parts of susceptible host plants (compatible interaction) and a hypersensitive reaction (HR) when infiltrated in nonhost plants (incompatible interaction). The HrpN(ea) harpin is a type three secretion system effector secreted by E. amylovora. This protein is involved in pathogenicity and HR-eliciting capacity of E. amylovora. In the present study, we showed that, in nonhost Arabidopsis thaliana cells, purified HrpN(ea) induces cell death and H(2)O(2) production, two nonhost resistance responses, but failed to induce such responses in host MM106 apple cells. Moreover, HrpN(ea) induced an increase in anion current in host MM106 apple cells, at the opposite of the decrease of anion current previously shown to be necessary to induce cell death in nonhost A. thaliana cells. These results suggest that HrpN(ea) induced different signaling pathways, which could account for early induced compatible or incompatible interaction development. |
| doi_str_mv | 10.1094/MPMI-20-0094 |
| format | Article |
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It provokes progressive necrosis in aerial parts of susceptible host plants (compatible interaction) and a hypersensitive reaction (HR) when infiltrated in nonhost plants (incompatible interaction). The HrpN(ea) harpin is a type three secretion system effector secreted by E. amylovora. This protein is involved in pathogenicity and HR-eliciting capacity of E. amylovora. In the present study, we showed that, in nonhost Arabidopsis thaliana cells, purified HrpN(ea) induces cell death and H(2)O(2) production, two nonhost resistance responses, but failed to induce such responses in host MM106 apple cells. Moreover, HrpN(ea) induced an increase in anion current in host MM106 apple cells, at the opposite of the decrease of anion current previously shown to be necessary to induce cell death in nonhost A. thaliana cells. These results suggest that HrpN(ea) induced different signaling pathways, which could account for early induced compatible or incompatible interaction development.</description><identifier>ISSN: 0894-0282</identifier><identifier>EISSN: 1943-7706</identifier><identifier>DOI: 10.1094/MPMI-20-0094</identifier><identifier>PMID: 17249426</identifier><language>eng</language><publisher>United States</publisher><subject>aerial parts ; apples ; Arabidopsis - cytology ; Arabidopsis - drug effects ; Arabidopsis - microbiology ; Arabidopsis thaliana ; bacterial diseases of plants ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Outer Membrane Proteins - pharmacology ; bacterial proteins ; Bacterial Proteins - genetics ; Bacterial Proteins - pharmacology ; biochemical pathways ; Cell Division - drug effects ; disease resistance ; Dose-Response Relationship, Drug ; Electrophysiology ; Erwinia amylovora ; Erwinia amylovora - genetics ; Erwinia amylovora - metabolism ; Erwinia amylovora - pathogenicity ; fruit crops ; harpins ; host plants ; host range ; hydrogen peroxide ; hypersensitive response ; in vitro studies ; ion transport ; Malus - cytology ; Malus - drug effects ; Malus - microbiology ; Malus domestica ; necrosis ; pathogenicity ; Pesticides - pharmacology ; plant pathogenic bacteria ; Potassium Channels - drug effects ; Potassium Channels - physiology ; Type III secretion system ; virulence</subject><ispartof>Molecular plant-microbe interactions, 2007, Vol.20 (1), p.94-100</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17249426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reboutier, D</creatorcontrib><creatorcontrib>Frankart, C</creatorcontrib><creatorcontrib>Briand, J</creatorcontrib><creatorcontrib>Biligui, B</creatorcontrib><creatorcontrib>Laroche, S</creatorcontrib><creatorcontrib>Rona, J.P</creatorcontrib><creatorcontrib>Barny, M.A</creatorcontrib><creatorcontrib>Bouteau, F</creatorcontrib><title>HrpN(ea) Harpin from Erwinia amylovora Triggers Differential Responses on the Nonhost Arabidopsis thaliana Cells and on the Host Apple Cells</title><title>Molecular plant-microbe interactions</title><addtitle>Mol Plant Microbe Interact</addtitle><description>Erwinia amylovora is a gram-negative necrogenic bacterium causing fire blight of the Maloideae subfamily of Rosaceae such as apple and pear. It provokes progressive necrosis in aerial parts of susceptible host plants (compatible interaction) and a hypersensitive reaction (HR) when infiltrated in nonhost plants (incompatible interaction). The HrpN(ea) harpin is a type three secretion system effector secreted by E. amylovora. This protein is involved in pathogenicity and HR-eliciting capacity of E. amylovora. In the present study, we showed that, in nonhost Arabidopsis thaliana cells, purified HrpN(ea) induces cell death and H(2)O(2) production, two nonhost resistance responses, but failed to induce such responses in host MM106 apple cells. Moreover, HrpN(ea) induced an increase in anion current in host MM106 apple cells, at the opposite of the decrease of anion current previously shown to be necessary to induce cell death in nonhost A. thaliana cells. These results suggest that HrpN(ea) induced different signaling pathways, which could account for early induced compatible or incompatible interaction development.</description><subject>aerial parts</subject><subject>apples</subject><subject>Arabidopsis - cytology</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - microbiology</subject><subject>Arabidopsis thaliana</subject><subject>bacterial diseases of plants</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Outer Membrane Proteins - pharmacology</subject><subject>bacterial proteins</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - pharmacology</subject><subject>biochemical pathways</subject><subject>Cell Division - drug effects</subject><subject>disease resistance</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electrophysiology</subject><subject>Erwinia amylovora</subject><subject>Erwinia amylovora - genetics</subject><subject>Erwinia amylovora - metabolism</subject><subject>Erwinia amylovora - pathogenicity</subject><subject>fruit crops</subject><subject>harpins</subject><subject>host plants</subject><subject>host range</subject><subject>hydrogen peroxide</subject><subject>hypersensitive response</subject><subject>in vitro studies</subject><subject>ion transport</subject><subject>Malus - cytology</subject><subject>Malus - drug effects</subject><subject>Malus - microbiology</subject><subject>Malus domestica</subject><subject>necrosis</subject><subject>pathogenicity</subject><subject>Pesticides - pharmacology</subject><subject>plant pathogenic bacteria</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - physiology</subject><subject>Type III secretion system</subject><subject>virulence</subject><issn>0894-0282</issn><issn>1943-7706</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kLFOwzAYhC0EoqWwMYNHGAKO7djJWJVCKrUFQTtHfxK7NUrtyA6gvgMPTURhOt3dpxsOocuY3MUk4_eLl8UsoiQivTlCwzjjLJKSiGM0JGnGI0JTOkBnIbwTEmciSU7RIJaUZ5yKIfrOfbu8UXCLc_CtsVh7t8NT_2WsAQy7feM-nQe88mazUT7gB6O18sp2Bhr8qkLrbFABO4u7rcJLZ7cudHjsoTS1a4MJfQ6NAQt4opomYLD1P53_om3bqEN3jk40NEFd_OkIrR-nq0kezZ-fZpPxPNKxYF2UQiartKZCatAsq2UqlZRKJFTQWpaK1wnjKSNES8poRZigulIUmC61JBVjI3R12G0_yp2qi9abHfh98X9LD1wfAA2ugI03oVi_URL3mzIRPEnZD2IfbqY</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Reboutier, D</creator><creator>Frankart, C</creator><creator>Briand, J</creator><creator>Biligui, B</creator><creator>Laroche, S</creator><creator>Rona, J.P</creator><creator>Barny, M.A</creator><creator>Bouteau, F</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>2007</creationdate><title>HrpN(ea) Harpin from Erwinia amylovora Triggers Differential Responses on the Nonhost Arabidopsis thaliana Cells and on the Host Apple Cells</title><author>Reboutier, D ; Frankart, C ; Briand, J ; Biligui, B ; Laroche, S ; Rona, J.P ; Barny, M.A ; Bouteau, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f163t-8a97c8d267faf39d787e77e65262d7be4d5348300f7232c0362fce2a3fbf70c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>aerial parts</topic><topic>apples</topic><topic>Arabidopsis - cytology</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - microbiology</topic><topic>Arabidopsis thaliana</topic><topic>bacterial diseases of plants</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Outer Membrane Proteins - pharmacology</topic><topic>bacterial proteins</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - pharmacology</topic><topic>biochemical pathways</topic><topic>Cell Division - drug effects</topic><topic>disease resistance</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electrophysiology</topic><topic>Erwinia amylovora</topic><topic>Erwinia amylovora - genetics</topic><topic>Erwinia amylovora - metabolism</topic><topic>Erwinia amylovora - pathogenicity</topic><topic>fruit crops</topic><topic>harpins</topic><topic>host plants</topic><topic>host range</topic><topic>hydrogen peroxide</topic><topic>hypersensitive response</topic><topic>in vitro studies</topic><topic>ion transport</topic><topic>Malus - cytology</topic><topic>Malus - drug effects</topic><topic>Malus - microbiology</topic><topic>Malus domestica</topic><topic>necrosis</topic><topic>pathogenicity</topic><topic>Pesticides - pharmacology</topic><topic>plant pathogenic bacteria</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>Type III secretion system</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reboutier, D</creatorcontrib><creatorcontrib>Frankart, C</creatorcontrib><creatorcontrib>Briand, J</creatorcontrib><creatorcontrib>Biligui, B</creatorcontrib><creatorcontrib>Laroche, S</creatorcontrib><creatorcontrib>Rona, J.P</creatorcontrib><creatorcontrib>Barny, M.A</creatorcontrib><creatorcontrib>Bouteau, F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Molecular plant-microbe interactions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reboutier, D</au><au>Frankart, C</au><au>Briand, J</au><au>Biligui, B</au><au>Laroche, S</au><au>Rona, J.P</au><au>Barny, M.A</au><au>Bouteau, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HrpN(ea) Harpin from Erwinia amylovora Triggers Differential Responses on the Nonhost Arabidopsis thaliana Cells and on the Host Apple Cells</atitle><jtitle>Molecular plant-microbe interactions</jtitle><addtitle>Mol Plant Microbe Interact</addtitle><date>2007</date><risdate>2007</risdate><volume>20</volume><issue>1</issue><spage>94</spage><epage>100</epage><pages>94-100</pages><issn>0894-0282</issn><eissn>1943-7706</eissn><abstract>Erwinia amylovora is a gram-negative necrogenic bacterium causing fire blight of the Maloideae subfamily of Rosaceae such as apple and pear. It provokes progressive necrosis in aerial parts of susceptible host plants (compatible interaction) and a hypersensitive reaction (HR) when infiltrated in nonhost plants (incompatible interaction). The HrpN(ea) harpin is a type three secretion system effector secreted by E. amylovora. This protein is involved in pathogenicity and HR-eliciting capacity of E. amylovora. In the present study, we showed that, in nonhost Arabidopsis thaliana cells, purified HrpN(ea) induces cell death and H(2)O(2) production, two nonhost resistance responses, but failed to induce such responses in host MM106 apple cells. Moreover, HrpN(ea) induced an increase in anion current in host MM106 apple cells, at the opposite of the decrease of anion current previously shown to be necessary to induce cell death in nonhost A. thaliana cells. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | aerial parts apples Arabidopsis - cytology Arabidopsis - drug effects Arabidopsis - microbiology Arabidopsis thaliana bacterial diseases of plants Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - pharmacology bacterial proteins Bacterial Proteins - genetics Bacterial Proteins - pharmacology biochemical pathways Cell Division - drug effects disease resistance Dose-Response Relationship, Drug Electrophysiology Erwinia amylovora Erwinia amylovora - genetics Erwinia amylovora - metabolism Erwinia amylovora - pathogenicity fruit crops harpins host plants host range hydrogen peroxide hypersensitive response in vitro studies ion transport Malus - cytology Malus - drug effects Malus - microbiology Malus domestica necrosis pathogenicity Pesticides - pharmacology plant pathogenic bacteria Potassium Channels - drug effects Potassium Channels - physiology Type III secretion system virulence |
| title | HrpN(ea) Harpin from Erwinia amylovora Triggers Differential Responses on the Nonhost Arabidopsis thaliana Cells and on the Host Apple Cells |
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