Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León
The aim of the present study was to determine the presence of Borrelia burgdorferi antibodies in horses from the metropolitan area of Monterrey, Nuevo León, México. Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on a...
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Veröffentlicht in: | Revista latinoamericana de microbiología (1970) 2001-10, Vol.43 (4), p.161 |
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creator | Salinas-Mélendez, J A Galván de la Garza, S Riojas-Valdés, V M Wong González, A Avalos-Ramírez, R |
description | The aim of the present study was to determine the presence of Borrelia burgdorferi antibodies in horses from the metropolitan area of Monterrey, Nuevo León, México. Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. All sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed by Western blot. The serological test yielded 34 positive sera at 1:64 dilution, and from them 6 were positive at 1:128 dilution, 3 at 1:256, and only one at 1:512. Confirmation of the infection by Western blot was obtained only in the sample positive at the 1:512 dilution. These results shown a low frequency of seropositivity to B. burgdorferi of the horses in the area, confirming previous studies indicating that in northeast Mexico Lyme disease is present in different animal species. |
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Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. All sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed by Western blot. The serological test yielded 34 positive sera at 1:64 dilution, and from them 6 were positive at 1:128 dilution, 3 at 1:256, and only one at 1:512. Confirmation of the infection by Western blot was obtained only in the sample positive at the 1:512 dilution. These results shown a low frequency of seropositivity to B. burgdorferi of the horses in the area, confirming previous studies indicating that in northeast Mexico Lyme disease is present in different animal species.</description><identifier>ISSN: 0187-4640</identifier><identifier>PMID: 17061503</identifier><language>eng</language><publisher>Mexico</publisher><subject>Animals ; Antibodies, Bacterial - blood ; Arachnid Vectors - microbiology ; Bites and Stings - complications ; Bites and Stings - microbiology ; Blotting, Western ; Borrelia burgdorferi - immunology ; Female ; Fluorescent Antibody Technique, Indirect ; Horse Diseases - diagnosis ; Horse Diseases - epidemiology ; Horse Diseases - transmission ; Horses - immunology ; Horses - parasitology ; Ixodes - microbiology ; Lyme Disease - diagnosis ; Lyme Disease - epidemiology ; Lyme Disease - transmission ; Lyme Disease - veterinary ; Male ; Mexico - epidemiology ; Tick Infestations - veterinary</subject><ispartof>Revista latinoamericana de microbiología (1970), 2001-10, Vol.43 (4), p.161</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17061503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salinas-Mélendez, J A</creatorcontrib><creatorcontrib>Galván de la Garza, S</creatorcontrib><creatorcontrib>Riojas-Valdés, V M</creatorcontrib><creatorcontrib>Wong González, A</creatorcontrib><creatorcontrib>Avalos-Ramírez, R</creatorcontrib><title>Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León</title><title>Revista latinoamericana de microbiología (1970)</title><addtitle>Rev Latinoam Microbiol</addtitle><description>The aim of the present study was to determine the presence of Borrelia burgdorferi antibodies in horses from the metropolitan area of Monterrey, Nuevo León, México. Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. All sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed by Western blot. The serological test yielded 34 positive sera at 1:64 dilution, and from them 6 were positive at 1:128 dilution, 3 at 1:256, and only one at 1:512. Confirmation of the infection by Western blot was obtained only in the sample positive at the 1:512 dilution. These results shown a low frequency of seropositivity to B. burgdorferi of the horses in the area, confirming previous studies indicating that in northeast Mexico Lyme disease is present in different animal species.</description><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Arachnid Vectors - microbiology</subject><subject>Bites and Stings - complications</subject><subject>Bites and Stings - microbiology</subject><subject>Blotting, Western</subject><subject>Borrelia burgdorferi - immunology</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Horse Diseases - diagnosis</subject><subject>Horse Diseases - epidemiology</subject><subject>Horse Diseases - transmission</subject><subject>Horses - immunology</subject><subject>Horses - parasitology</subject><subject>Ixodes - microbiology</subject><subject>Lyme Disease - diagnosis</subject><subject>Lyme Disease - epidemiology</subject><subject>Lyme Disease - transmission</subject><subject>Lyme Disease - veterinary</subject><subject>Male</subject><subject>Mexico - epidemiology</subject><subject>Tick Infestations - veterinary</subject><issn>0187-4640</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1j01OwzAUhL0A0ar0CsgHIJJ_0jhdloo_KcCm--q9-Lk1auNiO0g5F0fgYqQCZjPSaOaT5oJNhaxNUValmLB5Su_iLCOMXFyxiTSikguhp-xj1WWPwQ7cUqY2-9Bx2IHvUuZ3IUY6eODYx50N0VH03Hd8H2KixA-hhUz2nOQ98dSPNYRxHgkSD46_hC7TiBhu-WtPn4E39P3VXbNLB4dE8z-fsc3D_Wb9VDRvj8_rVVOcpFK5KJ1zWC5A0tKaGjRai62oKlkvsVWKFIAmROWMbE1dGUKnoK40CoGwNHrGbn6xpx6PZLen6I8Qh-3_df0D7x1Ycw</recordid><startdate>200110</startdate><enddate>200110</enddate><creator>Salinas-Mélendez, J A</creator><creator>Galván de la Garza, S</creator><creator>Riojas-Valdés, V M</creator><creator>Wong González, A</creator><creator>Avalos-Ramírez, R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>200110</creationdate><title>Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León</title><author>Salinas-Mélendez, J A ; Galván de la Garza, S ; Riojas-Valdés, V M ; Wong González, A ; Avalos-Ramírez, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p122t-4fffb45a1e9d78a3bddbc066189bc22e2aa3ebb2f71c7867ebf2a863b00ba973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Arachnid Vectors - microbiology</topic><topic>Bites and Stings - complications</topic><topic>Bites and Stings - microbiology</topic><topic>Blotting, Western</topic><topic>Borrelia burgdorferi - immunology</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Horse Diseases - diagnosis</topic><topic>Horse Diseases - epidemiology</topic><topic>Horse Diseases - transmission</topic><topic>Horses - immunology</topic><topic>Horses - parasitology</topic><topic>Ixodes - microbiology</topic><topic>Lyme Disease - diagnosis</topic><topic>Lyme Disease - epidemiology</topic><topic>Lyme Disease - transmission</topic><topic>Lyme Disease - veterinary</topic><topic>Male</topic><topic>Mexico - epidemiology</topic><topic>Tick Infestations - veterinary</topic><toplevel>online_resources</toplevel><creatorcontrib>Salinas-Mélendez, J A</creatorcontrib><creatorcontrib>Galván de la Garza, S</creatorcontrib><creatorcontrib>Riojas-Valdés, V M</creatorcontrib><creatorcontrib>Wong González, A</creatorcontrib><creatorcontrib>Avalos-Ramírez, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Revista latinoamericana de microbiología (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salinas-Mélendez, J A</au><au>Galván de la Garza, S</au><au>Riojas-Valdés, V M</au><au>Wong González, A</au><au>Avalos-Ramírez, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León</atitle><jtitle>Revista latinoamericana de microbiología (1970)</jtitle><addtitle>Rev Latinoam Microbiol</addtitle><date>2001-10</date><risdate>2001</risdate><volume>43</volume><issue>4</issue><spage>161</spage><pages>161-</pages><issn>0187-4640</issn><abstract>The aim of the present study was to determine the presence of Borrelia burgdorferi antibodies in horses from the metropolitan area of Monterrey, Nuevo León, México. Blood serum was obtained from a total of 100 horses residing at different counties in the area. From each animal data was obtained on age, sex, county of residence, presence of ectoparasites and clinical signs. All sera samples were analyzed by indirect immunofluoresence and the sera that resulted positive to this test was analyzed by Western blot. The serological test yielded 34 positive sera at 1:64 dilution, and from them 6 were positive at 1:128 dilution, 3 at 1:256, and only one at 1:512. Confirmation of the infection by Western blot was obtained only in the sample positive at the 1:512 dilution. These results shown a low frequency of seropositivity to B. burgdorferi of the horses in the area, confirming previous studies indicating that in northeast Mexico Lyme disease is present in different animal species.</abstract><cop>Mexico</cop><pmid>17061503</pmid></addata></record> |
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source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | Animals Antibodies, Bacterial - blood Arachnid Vectors - microbiology Bites and Stings - complications Bites and Stings - microbiology Blotting, Western Borrelia burgdorferi - immunology Female Fluorescent Antibody Technique, Indirect Horse Diseases - diagnosis Horse Diseases - epidemiology Horse Diseases - transmission Horses - immunology Horses - parasitology Ixodes - microbiology Lyme Disease - diagnosis Lyme Disease - epidemiology Lyme Disease - transmission Lyme Disease - veterinary Male Mexico - epidemiology Tick Infestations - veterinary |
title | Antibody detection against Borrelia burgdorferi in horses located in the suburban areas of Monterrey, Nuevo León |
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