Engineering Broad Root-Knot Resistance in Transgenic Plants by RNAi Silencing of a Conserved and Essential Root-Knot Nematode Parasitism Gene
Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative p...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2006-09, Vol.103 (39), p.14302-14306 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 14306 |
|---|---|
| container_issue | 39 |
| container_start_page | 14302 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 103 |
| creator | Huang, Guozhong Allen, Rex Davis, Eric L. Baum, Thomas J. Hussey, Richard S. |
| description | Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence this parasitism gene in RKN and validate that the parasitism gene has an essential function in RKN parasitism of plants. Ingestion of 16D10 dsRNA in vitro silenced the target parasitism gene in RKN and resulted in reduced nematode infectivity. In vivo expression of 16D10 dsRNA in Arabidopsis resulted in resistance effective against the four major RKN species. Because no known natural resistance gene has this wide effective range of RKN resistance, bioengineering crops expressing dsRNA that silence target RKN parasitism genes to disrupt the parasitic process represents a viable and flexible means of developing novel durable RKN-resistant crops and could provide crops with unprecedented broad resistance to RKN. |
| doi_str_mv | 10.1073/pnas.0604698103 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmed_primary_16985000</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30050369</jstor_id><sourcerecordid>30050369</sourcerecordid><originalsourceid>FETCH-LOGICAL-c596t-2aaeadaf784b7e93c8ac53b2270432d9d21e42bea7aaf57da3f65af60d48f3a13</originalsourceid><addsrcrecordid>eNqF0k1vEzEQBuAVAtFQOHMCWRyQOGw7Xu-HfalUolAQValCOVuT3dngaGMH21vRH8F_xlGipnDpybL8zKvxaLLsNYcTDo043VgMJ1BDWSvJQTzJJhwUz-tSwdNsAlA0uSyL8ih7EcIKAFQl4Xl2xBOv0nWS_ZnZpbFE3tgl--gddmzuXMy_WhfZnIIJEW1LzFh249GGJVnTsusBbQxsccfmV-eGfTcD2Xab4HqGbOpsIH9LHUPbsVkIZKPB4UHwFa0xuo7YNXoMJpqwZhdk6WX2rMch0Kv9eZz9-DS7mX7OL79dfJmeX-ZtpeqYF4iEHfaNLBcNKdFKbCuxKIoGSlF0qis4lcWCsEHsq6ZD0dcV9jV0pewFcnGcne1yN-NiTV2bGvQ46I03a_R32qHR_75Y81Mv3a3mVQNcling_T7Au18jhajXJrQ0pLmQG4OupQLOuXwUciUqKWSd4Lv_4MqN3qYp6AJ4IRtVNgmd7lDrXQie-vuWOejtQujtQujDQqSKtw9_evD7DUjgwx5sKw9xQguleSmg0P04DJF-x2TZIzaRNzuyCtH5eyMAKhC1En8BTmrWLQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>201287947</pqid></control><display><type>article</type><title>Engineering Broad Root-Knot Resistance in Transgenic Plants by RNAi Silencing of a Conserved and Essential Root-Knot Nematode Parasitism Gene</title><source>MEDLINE</source><source>Jstor Complete Legacy</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Huang, Guozhong ; Allen, Rex ; Davis, Eric L. ; Baum, Thomas J. ; Hussey, Richard S.</creator><creatorcontrib>Huang, Guozhong ; Allen, Rex ; Davis, Eric L. ; Baum, Thomas J. ; Hussey, Richard S.</creatorcontrib><description>Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence this parasitism gene in RKN and validate that the parasitism gene has an essential function in RKN parasitism of plants. Ingestion of 16D10 dsRNA in vitro silenced the target parasitism gene in RKN and resulted in reduced nematode infectivity. In vivo expression of 16D10 dsRNA in Arabidopsis resulted in resistance effective against the four major RKN species. Because no known natural resistance gene has this wide effective range of RKN resistance, bioengineering crops expressing dsRNA that silence target RKN parasitism genes to disrupt the parasitic process represents a viable and flexible means of developing novel durable RKN-resistant crops and could provide crops with unprecedented broad resistance to RKN.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0604698103</identifier><identifier>PMID: 16985000</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Animals ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - parasitology ; Base Sequence ; Biological Sciences ; Conserved Sequence - genetics ; Double stranded RNA ; Gene expression ; Gene Expression Regulation, Plant ; Genes ; Genes, Helminth - genetics ; Hybridity ; Molecular Sequence Data ; Nematoda ; Nematoda - genetics ; Nematodes ; Parasites ; Peptides ; Plant Diseases - genetics ; Plant parasitic nematodes ; Plant roots ; Plant Roots - parasitology ; Plants ; Plants, Genetically Modified ; Ribonucleic acid ; RNA ; RNA - metabolism ; RNA Interference ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Seedlings - metabolism ; Small interfering RNA ; Transgenic plants</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2006-09, Vol.103 (39), p.14302-14306</ispartof><rights>Copyright 2006 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Sep 26, 2006</rights><rights>2006 by The National Academy of Sciences of the USA 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-2aaeadaf784b7e93c8ac53b2270432d9d21e42bea7aaf57da3f65af60d48f3a13</citedby><cites>FETCH-LOGICAL-c596t-2aaeadaf784b7e93c8ac53b2270432d9d21e42bea7aaf57da3f65af60d48f3a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/103/39.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30050369$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30050369$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,728,781,785,804,886,27926,27927,53793,53795,58019,58252</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16985000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Guozhong</creatorcontrib><creatorcontrib>Allen, Rex</creatorcontrib><creatorcontrib>Davis, Eric L.</creatorcontrib><creatorcontrib>Baum, Thomas J.</creatorcontrib><creatorcontrib>Hussey, Richard S.</creatorcontrib><title>Engineering Broad Root-Knot Resistance in Transgenic Plants by RNAi Silencing of a Conserved and Essential Root-Knot Nematode Parasitism Gene</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence this parasitism gene in RKN and validate that the parasitism gene has an essential function in RKN parasitism of plants. Ingestion of 16D10 dsRNA in vitro silenced the target parasitism gene in RKN and resulted in reduced nematode infectivity. In vivo expression of 16D10 dsRNA in Arabidopsis resulted in resistance effective against the four major RKN species. Because no known natural resistance gene has this wide effective range of RKN resistance, bioengineering crops expressing dsRNA that silence target RKN parasitism genes to disrupt the parasitic process represents a viable and flexible means of developing novel durable RKN-resistant crops and could provide crops with unprecedented broad resistance to RKN.</description><subject>Animals</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - parasitology</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Conserved Sequence - genetics</subject><subject>Double stranded RNA</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Helminth - genetics</subject><subject>Hybridity</subject><subject>Molecular Sequence Data</subject><subject>Nematoda</subject><subject>Nematoda - genetics</subject><subject>Nematodes</subject><subject>Parasites</subject><subject>Peptides</subject><subject>Plant Diseases - genetics</subject><subject>Plant parasitic nematodes</subject><subject>Plant roots</subject><subject>Plant Roots - parasitology</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA - metabolism</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Seedlings - metabolism</subject><subject>Small interfering RNA</subject><subject>Transgenic plants</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0k1vEzEQBuAVAtFQOHMCWRyQOGw7Xu-HfalUolAQValCOVuT3dngaGMH21vRH8F_xlGipnDpybL8zKvxaLLsNYcTDo043VgMJ1BDWSvJQTzJJhwUz-tSwdNsAlA0uSyL8ih7EcIKAFQl4Xl2xBOv0nWS_ZnZpbFE3tgl--gddmzuXMy_WhfZnIIJEW1LzFh249GGJVnTsusBbQxsccfmV-eGfTcD2Xab4HqGbOpsIH9LHUPbsVkIZKPB4UHwFa0xuo7YNXoMJpqwZhdk6WX2rMch0Kv9eZz9-DS7mX7OL79dfJmeX-ZtpeqYF4iEHfaNLBcNKdFKbCuxKIoGSlF0qis4lcWCsEHsq6ZD0dcV9jV0pewFcnGcne1yN-NiTV2bGvQ46I03a_R32qHR_75Y81Mv3a3mVQNcling_T7Au18jhajXJrQ0pLmQG4OupQLOuXwUciUqKWSd4Lv_4MqN3qYp6AJ4IRtVNgmd7lDrXQie-vuWOejtQujtQujDQqSKtw9_evD7DUjgwx5sKw9xQguleSmg0P04DJF-x2TZIzaRNzuyCtH5eyMAKhC1En8BTmrWLQ</recordid><startdate>20060926</startdate><enddate>20060926</enddate><creator>Huang, Guozhong</creator><creator>Allen, Rex</creator><creator>Davis, Eric L.</creator><creator>Baum, Thomas J.</creator><creator>Hussey, Richard S.</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060926</creationdate><title>Engineering Broad Root-Knot Resistance in Transgenic Plants by RNAi Silencing of a Conserved and Essential Root-Knot Nematode Parasitism Gene</title><author>Huang, Guozhong ; Allen, Rex ; Davis, Eric L. ; Baum, Thomas J. ; Hussey, Richard S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-2aaeadaf784b7e93c8ac53b2270432d9d21e42bea7aaf57da3f65af60d48f3a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - parasitology</topic><topic>Base Sequence</topic><topic>Biological Sciences</topic><topic>Conserved Sequence - genetics</topic><topic>Double stranded RNA</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes, Helminth - genetics</topic><topic>Hybridity</topic><topic>Molecular Sequence Data</topic><topic>Nematoda</topic><topic>Nematoda - genetics</topic><topic>Nematodes</topic><topic>Parasites</topic><topic>Peptides</topic><topic>Plant Diseases - genetics</topic><topic>Plant parasitic nematodes</topic><topic>Plant roots</topic><topic>Plant Roots - parasitology</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA - metabolism</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Seedlings - metabolism</topic><topic>Small interfering RNA</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Guozhong</creatorcontrib><creatorcontrib>Allen, Rex</creatorcontrib><creatorcontrib>Davis, Eric L.</creatorcontrib><creatorcontrib>Baum, Thomas J.</creatorcontrib><creatorcontrib>Hussey, Richard S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Guozhong</au><au>Allen, Rex</au><au>Davis, Eric L.</au><au>Baum, Thomas J.</au><au>Hussey, Richard S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineering Broad Root-Knot Resistance in Transgenic Plants by RNAi Silencing of a Conserved and Essential Root-Knot Nematode Parasitism Gene</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2006-09-26</date><risdate>2006</risdate><volume>103</volume><issue>39</issue><spage>14302</spage><epage>14306</epage><pages>14302-14306</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Secreted parasitism proteins encoded by parasitism genes expressed in esophageal gland cells mediate infection and parasitism of plants by root-knot nematodes (RKN). Parasitism gene 16D10 encodes a conserved RKN secretory peptide that stimulates root growth and functions as a ligand for a putative plant transcription factor. We used in vitro and in vivo RNA interference approaches to silence this parasitism gene in RKN and validate that the parasitism gene has an essential function in RKN parasitism of plants. Ingestion of 16D10 dsRNA in vitro silenced the target parasitism gene in RKN and resulted in reduced nematode infectivity. In vivo expression of 16D10 dsRNA in Arabidopsis resulted in resistance effective against the four major RKN species. Because no known natural resistance gene has this wide effective range of RKN resistance, bioengineering crops expressing dsRNA that silence target RKN parasitism genes to disrupt the parasitic process represents a viable and flexible means of developing novel durable RKN-resistant crops and could provide crops with unprecedented broad resistance to RKN.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>16985000</pmid><doi>10.1073/pnas.0604698103</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2006-09, Vol.103 (39), p.14302-14306 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_16985000 |
| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Arabidopsis Arabidopsis - genetics Arabidopsis - parasitology Base Sequence Biological Sciences Conserved Sequence - genetics Double stranded RNA Gene expression Gene Expression Regulation, Plant Genes Genes, Helminth - genetics Hybridity Molecular Sequence Data Nematoda Nematoda - genetics Nematodes Parasites Peptides Plant Diseases - genetics Plant parasitic nematodes Plant roots Plant Roots - parasitology Plants Plants, Genetically Modified Ribonucleic acid RNA RNA - metabolism RNA Interference RNA, Messenger - genetics RNA, Messenger - metabolism Seedlings - metabolism Small interfering RNA Transgenic plants |
| title | Engineering Broad Root-Knot Resistance in Transgenic Plants by RNAi Silencing of a Conserved and Essential Root-Knot Nematode Parasitism Gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T10%3A48%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Engineering%20Broad%20Root-Knot%20Resistance%20in%20Transgenic%20Plants%20by%20RNAi%20Silencing%20of%20a%20Conserved%20and%20Essential%20Root-Knot%20Nematode%20Parasitism%20Gene&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Huang,%20Guozhong&rft.date=2006-09-26&rft.volume=103&rft.issue=39&rft.spage=14302&rft.epage=14306&rft.pages=14302-14306&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.0604698103&rft_dat=%3Cjstor_pubme%3E30050369%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=201287947&rft_id=info:pmid/16985000&rft_jstor_id=30050369&rfr_iscdi=true |