Radiotoxicity of 125I-Iododeoxyuridine in Pre-implantation Mouse Embryos
Summary The radiotoxicity of DNA incorporated 125I in cultured pre-implantation two-cell mouse embryos was investigated and compared with external γ-irradiation. The uptake of 125IdU in the two-cell stage embryos was determined as a function of incubation time and concentration of radioactivity (MBq...
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| Veröffentlicht in: | International journal of radiation biology 1991-09, Vol.60 (3), p.525-532 |
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| container_end_page | 532 |
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| container_issue | 3 |
| container_start_page | 525 |
| container_title | International journal of radiation biology |
| container_volume | 60 |
| creator | Narra, Venkateswara R. Howell, Roger W. Thanki, Kantilal H. Rao, Dandamudi V. |
| description | Summary
The radiotoxicity of DNA incorporated 125I in cultured pre-implantation two-cell mouse embryos was investigated and compared with external γ-irradiation. The uptake of 125IdU in the two-cell stage embryos was determined as a function of incubation time and concentration of radioactivity (MBq/ml) in the medium. The absorbed dose to the embryos was calculated using conventional procedures. The embryo survival curves show that the dose at 37% survival is only about 15 cGy for 125IdU, whereas for 137Cs-photons it is 175 cGy. The extreme toxicity observed is thought to be due to the localized energy deposition of the numerous low energy Auger electrons emitted in the decay of 125I. These results are consistent with earlier observations in mouse testis and cultured cells and point to the need for assessing the radiation risk from incorporated Auger electron emitting radionuclides based on their subcellular distribution. |
| doi_str_mv | 10.1080/09553009114552361 |
| format | Article |
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The radiotoxicity of DNA incorporated 125I in cultured pre-implantation two-cell mouse embryos was investigated and compared with external γ-irradiation. The uptake of 125IdU in the two-cell stage embryos was determined as a function of incubation time and concentration of radioactivity (MBq/ml) in the medium. The absorbed dose to the embryos was calculated using conventional procedures. The embryo survival curves show that the dose at 37% survival is only about 15 cGy for 125IdU, whereas for 137Cs-photons it is 175 cGy. The extreme toxicity observed is thought to be due to the localized energy deposition of the numerous low energy Auger electrons emitted in the decay of 125I. These results are consistent with earlier observations in mouse testis and cultured cells and point to the need for assessing the radiation risk from incorporated Auger electron emitting radionuclides based on their subcellular distribution.</description><identifier>ISSN: 0955-3002</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553009114552361</identifier><identifier>PMID: 1679091</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Cell Survival - radiation effects ; Dose-Response Relationship, Radiation ; Embryo, Mammalian - metabolism ; Embryo, Mammalian - radiation effects ; Female ; Idoxuridine - pharmacokinetics ; In Vitro Techniques ; Iodine Radioisotopes ; Mice</subject><ispartof>International journal of radiation biology, 1991-09, Vol.60 (3), p.525-532</ispartof><rights>1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1991</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553009114552361$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553009114552361$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,59624,59730,60413,60519,61198,61233,61379,61414</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1679091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Narra, Venkateswara R.</creatorcontrib><creatorcontrib>Howell, Roger W.</creatorcontrib><creatorcontrib>Thanki, Kantilal H.</creatorcontrib><creatorcontrib>Rao, Dandamudi V.</creatorcontrib><title>Radiotoxicity of 125I-Iododeoxyuridine in Pre-implantation Mouse Embryos</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol</addtitle><description>Summary
The radiotoxicity of DNA incorporated 125I in cultured pre-implantation two-cell mouse embryos was investigated and compared with external γ-irradiation. The uptake of 125IdU in the two-cell stage embryos was determined as a function of incubation time and concentration of radioactivity (MBq/ml) in the medium. The absorbed dose to the embryos was calculated using conventional procedures. The embryo survival curves show that the dose at 37% survival is only about 15 cGy for 125IdU, whereas for 137Cs-photons it is 175 cGy. The extreme toxicity observed is thought to be due to the localized energy deposition of the numerous low energy Auger electrons emitted in the decay of 125I. These results are consistent with earlier observations in mouse testis and cultured cells and point to the need for assessing the radiation risk from incorporated Auger electron emitting radionuclides based on their subcellular distribution.</description><subject>Animals</subject><subject>Cell Survival - radiation effects</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Embryo, Mammalian - radiation effects</subject><subject>Female</subject><subject>Idoxuridine - pharmacokinetics</subject><subject>In Vitro Techniques</subject><subject>Iodine Radioisotopes</subject><subject>Mice</subject><issn>0955-3002</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1LwzAUhoMoc05_gBdC_0A1H02XojcyNjeYKKLXIU1OWEbbjLTF9d-bsd2IeHVeeHgOvC9CtwTfEyzwAy44ZxgXhGScU5aTMzQmLKcpi-QcjQ88Zkwv0VXbbnFMmIkRGpF8WkRtjJYfyjjf-b3TrhsSbxNC-SpdeeMN-P3QB2dcA4lrkvcAqat3lWo61TnfJK--byGZ12UYfHuNLqyqWrg53Qn6Wsw_Z8t0_faymj2vU0e4IGkuoAQ8pRqX3BRlJoAToFyBZtpmFhutY6WCliCmkGluM01yQYtCA7ZaUDZBd8e_u76swchdcLUKgzw1ivzpyF1jfajVtw-VkZ0aKh9sUI12rWQEy8N-8s9-UX_8pW9AVd1GqwBy6_vQxGryf_sHU3tzdA</recordid><startdate>199109</startdate><enddate>199109</enddate><creator>Narra, Venkateswara R.</creator><creator>Howell, Roger W.</creator><creator>Thanki, Kantilal H.</creator><creator>Rao, Dandamudi V.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>199109</creationdate><title>Radiotoxicity of 125I-Iododeoxyuridine in Pre-implantation Mouse Embryos</title><author>Narra, Venkateswara R. ; Howell, Roger W. ; Thanki, Kantilal H. ; Rao, Dandamudi V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i1581-68ebe072c0b5d9b48e51e25aec3cf4f0dcc55392be87e4c5f4c168299ce0fc823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animals</topic><topic>Cell Survival - radiation effects</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Embryo, Mammalian - radiation effects</topic><topic>Female</topic><topic>Idoxuridine - pharmacokinetics</topic><topic>In Vitro Techniques</topic><topic>Iodine Radioisotopes</topic><topic>Mice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Narra, Venkateswara R.</creatorcontrib><creatorcontrib>Howell, Roger W.</creatorcontrib><creatorcontrib>Thanki, Kantilal H.</creatorcontrib><creatorcontrib>Rao, Dandamudi V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Narra, Venkateswara R.</au><au>Howell, Roger W.</au><au>Thanki, Kantilal H.</au><au>Rao, Dandamudi V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radiotoxicity of 125I-Iododeoxyuridine in Pre-implantation Mouse Embryos</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol</addtitle><date>1991-09</date><risdate>1991</risdate><volume>60</volume><issue>3</issue><spage>525</spage><epage>532</epage><pages>525-532</pages><issn>0955-3002</issn><eissn>1362-3095</eissn><abstract>Summary
The radiotoxicity of DNA incorporated 125I in cultured pre-implantation two-cell mouse embryos was investigated and compared with external γ-irradiation. The uptake of 125IdU in the two-cell stage embryos was determined as a function of incubation time and concentration of radioactivity (MBq/ml) in the medium. The absorbed dose to the embryos was calculated using conventional procedures. The embryo survival curves show that the dose at 37% survival is only about 15 cGy for 125IdU, whereas for 137Cs-photons it is 175 cGy. The extreme toxicity observed is thought to be due to the localized energy deposition of the numerous low energy Auger electrons emitted in the decay of 125I. These results are consistent with earlier observations in mouse testis and cultured cells and point to the need for assessing the radiation risk from incorporated Auger electron emitting radionuclides based on their subcellular distribution.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>1679091</pmid><doi>10.1080/09553009114552361</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0955-3002 |
| ispartof | International journal of radiation biology, 1991-09, Vol.60 (3), p.525-532 |
| issn | 0955-3002 1362-3095 |
| language | eng |
| recordid | cdi_pubmed_primary_1679091 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Animals Cell Survival - radiation effects Dose-Response Relationship, Radiation Embryo, Mammalian - metabolism Embryo, Mammalian - radiation effects Female Idoxuridine - pharmacokinetics In Vitro Techniques Iodine Radioisotopes Mice |
| title | Radiotoxicity of 125I-Iododeoxyuridine in Pre-implantation Mouse Embryos |
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