Reliability of Detecting rRNA Sequences of Chlamydia trachomatis With Fluorescence in Situ Hybridization Without Amplification

To use fluorescence in situ hybridization (FISH) using ribosomal RNA (rRNA) oligonucleotide probes as the target nucleic acid for the detection of Chlamydia trachomatis. Suitable sequences selected from the rRNA sequence of C trachomatis were labeled with a fluorescent dye and used in FISH for detec...

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Veröffentlicht in:	Acta cytologica 2006-05, Vol.50 (3), p.277-283
Hauptverfasser:	Kapur, Sujala, Ahmed, Margoob, Singh, Vineeta, Krupp, Guido, Salhan, Sudha, Mittal, Aruna
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Typing Techniques Biological and medical sciences Cervix Uteri - microbiology Chlamydia Infections - diagnosis Chlamydia Infections - microbiology Chlamydia trachomatis - genetics Chlamydia trachomatis - isolation & purification Female Humans In Situ Hybridization, Fluorescence - methods Investigative techniques, diagnostic techniques (general aspects) Medical sciences Molecular Techniques Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Reproducibility of Results RNA Probes RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Urethra - microbiology
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