Reactions of Hydrated Electrons with Horseradish Peroxidase: A Pulse Radiolysis Study
Summary Kinetics of the reactions of hydrated electrons with horseradish peroxidase (HRP) have been studied by pulse radiolysis. Hydrated electrons reduce HRP to its ferrous form with a yield of 40%. At higher dose (250 Gy) the formation of compound I followed by its reduction to compound II was als...
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| Veröffentlicht in: | International journal of radiation biology 1991, Vol.59 (3), p.617-623 |
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| container_issue | 3 |
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| container_title | International journal of radiation biology |
| container_volume | 59 |
| creator | G bicka, L. G bicki, J.L. |
| description | Summary
Kinetics of the reactions of hydrated electrons with horseradish peroxidase (HRP) have been studied by pulse radiolysis. Hydrated electrons reduce HRP to its ferrous form with a yield of 40%. At higher dose (250 Gy) the formation of compound I followed by its reduction to compound II was also observed. |
| doi_str_mv | 10.1080/09553009114550551 |
| format | Article |
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Kinetics of the reactions of hydrated electrons with horseradish peroxidase (HRP) have been studied by pulse radiolysis. Hydrated electrons reduce HRP to its ferrous form with a yield of 40%. At higher dose (250 Gy) the formation of compound I followed by its reduction to compound II was also observed.</description><identifier>ISSN: 0955-3002</identifier><identifier>EISSN: 1362-3095</identifier><identifier>DOI: 10.1080/09553009114550551</identifier><identifier>PMID: 1672352</identifier><language>eng</language><publisher>London: Informa UK Ltd</publisher><subject>Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Electrons ; Free Radicals ; Fundamental and applied biological sciences. Psychology ; Horseradish Peroxidase ; Methods. Procedures. Technologies ; Pulse Radiolysis ; Space life sciences</subject><ispartof>International journal of radiation biology, 1991, Vol.59 (3), p.617-623</ispartof><rights>1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-7ce8b169c3cb43a455a9548dc4a1eda1fd44231a0db21a0f8f061ae7cbab64f23</citedby><cites>FETCH-LOGICAL-c431t-7ce8b169c3cb43a455a9548dc4a1eda1fd44231a0db21a0f8f061ae7cbab64f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/09553009114550551$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/09553009114550551$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,777,781,4010,27904,27905,27906,59626,59732,60415,60521,61200,61235,61381,61416</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19671786$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1672352$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>G bicka, L.</creatorcontrib><creatorcontrib>G bicki, J.L.</creatorcontrib><title>Reactions of Hydrated Electrons with Horseradish Peroxidase: A Pulse Radiolysis Study</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol</addtitle><description>Summary
Kinetics of the reactions of hydrated electrons with horseradish peroxidase (HRP) have been studied by pulse radiolysis. Hydrated electrons reduce HRP to its ferrous form with a yield of 40%. At higher dose (250 Gy) the formation of compound I followed by its reduction to compound II was also observed.</description><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Electrons</subject><subject>Free Radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Horseradish Peroxidase</subject><subject>Methods. Procedures. Technologies</subject><subject>Pulse Radiolysis</subject><subject>Space life sciences</subject><issn>0955-3002</issn><issn>1362-3095</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9rVDEQxYModa1-AB-EvOjbauYm95_6Ukp1hYKl2ufL3GTCpmRvapJLvd_eLLtSROjLzJDfOUPmMPYaxHsQnfgg-rqWQvQAqq5FXcMTtgLZVGtZyFO22vMyi-o5e5HSrSiTkN0JO4GmrWRdrdjNNaHOLkyJB8s3i4mYyfALTzrH_eu9y1u-CTFRROPSll9RDL-dwUQf-Rm_mn0ifl1Q8Etyif_Is1lesmcWC3h17Kfs5svFz_PN-vL712_nZ5drrSTkdaupG6HptdSjklhuwL5WndEKgQyCNUpVElCYsSrVdlY0gNTqEcdG2UqesneHvXcx_Jop5WHnkibvcaIwp6ETqlPQQBHCQahjSCmSHe6i22FcBhDDPsrhvyiL581x-TzuyDw4DtkV_vbIMWn0NuKkXXqQ9U0LbdcU3eeDzk02xB3eh-jNkHHxIf41yce-8ekf-5bQ563GSMNtmONU8n3kiD9Vs6EQ</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>G bicka, L.</creator><creator>G bicki, J.L.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Reactions of Hydrated Electrons with Horseradish Peroxidase: A Pulse Radiolysis Study</title><author>G bicka, L. ; G bicki, J.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-7ce8b169c3cb43a455a9548dc4a1eda1fd44231a0db21a0f8f061ae7cbab64f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Electrons</topic><topic>Free Radicals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Horseradish Peroxidase</topic><topic>Methods. Procedures. Technologies</topic><topic>Pulse Radiolysis</topic><topic>Space life sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>G bicka, L.</creatorcontrib><creatorcontrib>G bicki, J.L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>G bicka, L.</au><au>G bicki, J.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reactions of Hydrated Electrons with Horseradish Peroxidase: A Pulse Radiolysis Study</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol</addtitle><date>1991</date><risdate>1991</risdate><volume>59</volume><issue>3</issue><spage>617</spage><epage>623</epage><pages>617-623</pages><issn>0955-3002</issn><eissn>1362-3095</eissn><abstract>Summary
Kinetics of the reactions of hydrated electrons with horseradish peroxidase (HRP) have been studied by pulse radiolysis. Hydrated electrons reduce HRP to its ferrous form with a yield of 40%. At higher dose (250 Gy) the formation of compound I followed by its reduction to compound II was also observed.</abstract><cop>London</cop><pub>Informa UK Ltd</pub><pmid>1672352</pmid><doi>10.1080/09553009114550551</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0955-3002 |
| ispartof | International journal of radiation biology, 1991, Vol.59 (3), p.617-623 |
| issn | 0955-3002 1362-3095 |
| language | eng |
| recordid | cdi_pubmed_primary_1672352 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Electrons Free Radicals Fundamental and applied biological sciences. Psychology Horseradish Peroxidase Methods. Procedures. Technologies Pulse Radiolysis Space life sciences |
| title | Reactions of Hydrated Electrons with Horseradish Peroxidase: A Pulse Radiolysis Study |
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