Stable genetic transformation of intact Nicotiana cells by the particle bombardment process

We show that the genetic transformation of Nicotiana tabacum can be achieved by bombarding intact cells and tissues with DNA-coated particles. Leaves or suspension culture cells were treated with tungsten microprojectiles carrying plasmid DNA containing a neomycin phosphotransferase gene. Callus har...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1988-11, Vol.85 (22), p.8502-8505
Hauptverfasser:	Klein, T.M, Harper, E.C, Svab, Z, Sanford, J.C, Fromm, M.E, Maliga, P
Format:	Artikel
Sprache:	eng
Schlagworte:	BIOLOGIA Biological and medical sciences Biological Sciences: Botany BIOLOGIE BIOLOGY Cell culture techniques Chemical suspensions Classical genetics, quantitative genetics, hybrids Cultured cells Diploidy DNA Fundamental and applied biological sciences. Psychology GENE GENES GENETIC ENGINEERING GENETIC TRANSFORMATION Genetics of eukaryotes. Biological and molecular evolution GENIE GENETIQUE INGENIERIA GENETICA Leaves Methods, theories and miscellaneous microprojectiles NICOTIANA TABACUM particle gun Plant cells Plants Plasmids TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE transgenic plants
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