Combined proton NMR imaging and spectral analysis of locust embryonic development

Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collected in vivo using a 4.7-tesla proton (1H) NMR imaging spectrometer. The 100 × 100 × 500 μ m image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embr...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1987-08, Vol.84 (15), p.5297-5300
Hauptverfasser: Gassner, G, Lohman, J.A.B
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description Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collected in vivo using a 4.7-tesla proton (1H) NMR imaging spectrometer. The 100 × 100 × 500 μ m image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embryonic development were slow enough that the time delay between imaging and spectral measurements in negligible. Thus, image and spectral data correspond closely and approximate real-time observations of embryonic changes. The above procedure was applied every 12 hr during the entire course of development. This analytical approach demonstrates that imaging and localized spectroscopy can be used to visualize and assess changes in embryonic water and lipid content in concert with the development of a living embryo.
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Psychology</topic><topic>General aspects, investigation technics, apparatus</topic><topic>Graphics</topic><topic>Imaging</topic><topic>Lipids</topic><topic>Locusts</topic><topic>NMR SPECTROSCOPY</topic><topic>Protons</topic><topic>SCHISTOCERCA</topic><topic>SCHISTOCERCA GREGARIA</topic><topic>SPECTROSCOPIE RMN</topic><topic>Spectroscopy</topic><topic>Tissues, organs and organisms biophysics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gassner, G</creatorcontrib><creatorcontrib>Lohman, J.A.B</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gassner, G</au><au>Lohman, J.A.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined proton NMR imaging and spectral analysis of locust embryonic development</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-08-01</date><risdate>1987</risdate><volume>84</volume><issue>15</issue><spage>5297</spage><epage>5300</epage><pages>5297-5300</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collected in vivo using a 4.7-tesla proton (1H) NMR imaging spectrometer. 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subjects Acrididae
Agriculture
Biological and medical sciences
Biological Sciences: Developmental Biology
DESARROLLO EMBRIONARIO
Developmental biology
DEVELOPPEMENT EMBRYONNAIRE
Eggs
EMBRYONIC DEVELOPMENT
Embryos
ESPECTROSCOPIA RMN
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
Graphics
Imaging
Lipids
Locusts
NMR SPECTROSCOPY
Protons
SCHISTOCERCA
SCHISTOCERCA GREGARIA
SPECTROSCOPIE RMN
Spectroscopy
Tissues, organs and organisms biophysics
title Combined proton NMR imaging and spectral analysis of locust embryonic development
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