Combined proton NMR imaging and spectral analysis of locust embryonic development
Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collected in vivo using a 4.7-tesla proton (1H) NMR imaging spectrometer. The 100 × 100 × 500 μ m image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embr...
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Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1987-08, Vol.84 (15), p.5297-5300 |
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description | Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collected in vivo using a 4.7-tesla proton (1H) NMR imaging spectrometer. The 100 × 100 × 500 μ m image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embryonic development were slow enough that the time delay between imaging and spectral measurements in negligible. Thus, image and spectral data correspond closely and approximate real-time observations of embryonic changes. The above procedure was applied every 12 hr during the entire course of development. This analytical approach demonstrates that imaging and localized spectroscopy can be used to visualize and assess changes in embryonic water and lipid content in concert with the development of a living embryo. |
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The 100 × 100 × 500 μ m image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embryonic development were slow enough that the time delay between imaging and spectral measurements in negligible. Thus, image and spectral data correspond closely and approximate real-time observations of embryonic changes. The above procedure was applied every 12 hr during the entire course of development. This analytical approach demonstrates that imaging and localized spectroscopy can be used to visualize and assess changes in embryonic water and lipid content in concert with the development of a living embryo.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.15.5297</identifier><identifier>PMID: 16593861</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Acrididae ; Agriculture ; Biological and medical sciences ; Biological Sciences: Developmental Biology ; DESARROLLO EMBRIONARIO ; Developmental biology ; DEVELOPPEMENT EMBRYONNAIRE ; Eggs ; EMBRYONIC DEVELOPMENT ; Embryos ; ESPECTROSCOPIA RMN ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation technics, apparatus ; Graphics ; Imaging ; Lipids ; Locusts ; NMR SPECTROSCOPY ; Protons ; SCHISTOCERCA ; SCHISTOCERCA GREGARIA ; SPECTROSCOPIE RMN ; Spectroscopy ; Tissues, organs and organisms biophysics</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-08, Vol.84 (15), p.5297-5300</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-e09dd2993594e7e8e49a63b287dfe2c3486f41973694f7ffe0d357de6821194d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/15.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30024$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30024$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8333928$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16593861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gassner, G</creatorcontrib><creatorcontrib>Lohman, J.A.B</creatorcontrib><title>Combined proton NMR imaging and spectral analysis of locust embryonic development</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collected in vivo using a 4.7-tesla proton (1H) NMR imaging spectrometer. The 100 × 100 × 500 μ m image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embryonic development were slow enough that the time delay between imaging and spectral measurements in negligible. Thus, image and spectral data correspond closely and approximate real-time observations of embryonic changes. The above procedure was applied every 12 hr during the entire course of development. This analytical approach demonstrates that imaging and localized spectroscopy can be used to visualize and assess changes in embryonic water and lipid content in concert with the development of a living embryo.</description><subject>Acrididae</subject><subject>Agriculture</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences: Developmental Biology</subject><subject>DESARROLLO EMBRIONARIO</subject><subject>Developmental biology</subject><subject>DEVELOPPEMENT EMBRYONNAIRE</subject><subject>Eggs</subject><subject>EMBRYONIC DEVELOPMENT</subject><subject>Embryos</subject><subject>ESPECTROSCOPIA RMN</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation technics, apparatus</subject><subject>Graphics</subject><subject>Imaging</subject><subject>Lipids</subject><subject>Locusts</subject><subject>NMR SPECTROSCOPY</subject><subject>Protons</subject><subject>SCHISTOCERCA</subject><subject>SCHISTOCERCA GREGARIA</subject><subject>SPECTROSCOPIE RMN</subject><subject>Spectroscopy</subject><subject>Tissues, organs and organisms biophysics</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNp9kkuLFDEUhYMoTs_oWhCRWoiuqievqiQLF9KMDxgVH7MO6TzaGlJJmVQN9r83RTdlC-IqhPvde07uCQBPEFwjyMjlEFRec7pGzbrBgt0DKwQFqlsq4H2wghCzmlNMz8B5zrcQQtFw-BCcobYRhLdoBb5sYr_tgjXVkOIYQ_Xp49eq69WuC7tKBVPlweoxKV8uyu9zl6voKh_1lMfK9tu0j6HTlbF31seht2F8BB445bN9fDwvwM3bq--b9_X153cfNm-ua01ZO9YWCmOwEKQR1DLLLRWqJVvMmXEWa0J56ygSjLSCOuachYY0zNiWY4QENeQCvD7MHaZtb40u0sWmHFJxn_Yyqk7-XQndD7mLdxILXnZS-l8d-1P8Odk8yr7L2nqvgo1TloyQBiLEaCFf_pdElJeZcB55eQB1ijkn6xY3CMo5LznnJTmVqJFzXqXj-ekj_vDHgArw4giorJV3SQXd5YXjhBCB-cljZoGlughJN3k_2l_jieK_yQI8PQC3eYxpIUj5TPMqnh2KTkWpdqlYufnGWYOJKFslvwHd1sjM</recordid><startdate>19870801</startdate><enddate>19870801</enddate><creator>Gassner, G</creator><creator>Lohman, J.A.B</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870801</creationdate><title>Combined proton NMR imaging and spectral analysis of locust embryonic development</title><author>Gassner, G ; Lohman, J.A.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-e09dd2993594e7e8e49a63b287dfe2c3486f41973694f7ffe0d357de6821194d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Acrididae</topic><topic>Agriculture</topic><topic>Biological and medical sciences</topic><topic>Biological Sciences: Developmental Biology</topic><topic>DESARROLLO EMBRIONARIO</topic><topic>Developmental biology</topic><topic>DEVELOPPEMENT EMBRYONNAIRE</topic><topic>Eggs</topic><topic>EMBRYONIC DEVELOPMENT</topic><topic>Embryos</topic><topic>ESPECTROSCOPIA RMN</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation technics, apparatus</topic><topic>Graphics</topic><topic>Imaging</topic><topic>Lipids</topic><topic>Locusts</topic><topic>NMR SPECTROSCOPY</topic><topic>Protons</topic><topic>SCHISTOCERCA</topic><topic>SCHISTOCERCA GREGARIA</topic><topic>SPECTROSCOPIE RMN</topic><topic>Spectroscopy</topic><topic>Tissues, organs and organisms biophysics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gassner, G</creatorcontrib><creatorcontrib>Lohman, J.A.B</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gassner, G</au><au>Lohman, J.A.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined proton NMR imaging and spectral analysis of locust embryonic development</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-08-01</date><risdate>1987</risdate><volume>84</volume><issue>15</issue><spage>5297</spage><epage>5300</epage><pages>5297-5300</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Images and spectra of desert locust [Schistocerca gregaria (Forskal)] embryos were collected in vivo using a 4.7-tesla proton (1H) NMR imaging spectrometer. The 100 × 100 × 500 μ m image resolution and 125-nl localized spectral volumes were obtained within minutes of each other. The dynamics of embryonic development were slow enough that the time delay between imaging and spectral measurements in negligible. Thus, image and spectral data correspond closely and approximate real-time observations of embryonic changes. The above procedure was applied every 12 hr during the entire course of development. This analytical approach demonstrates that imaging and localized spectroscopy can be used to visualize and assess changes in embryonic water and lipid content in concert with the development of a living embryo.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>16593861</pmid><doi>10.1073/pnas.84.15.5297</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Acrididae Agriculture Biological and medical sciences Biological Sciences: Developmental Biology DESARROLLO EMBRIONARIO Developmental biology DEVELOPPEMENT EMBRYONNAIRE Eggs EMBRYONIC DEVELOPMENT Embryos ESPECTROSCOPIA RMN Fundamental and applied biological sciences. Psychology General aspects, investigation technics, apparatus Graphics Imaging Lipids Locusts NMR SPECTROSCOPY Protons SCHISTOCERCA SCHISTOCERCA GREGARIA SPECTROSCOPIE RMN Spectroscopy Tissues, organs and organisms biophysics |
title | Combined proton NMR imaging and spectral analysis of locust embryonic development |
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