Differences in Leucyl-Transfer RNA's and Synthetase in Soybean Seedlings
Charged leucyl-transfer RNA (leucyl-tRNA) of soybean cotyledons can be fractionated into six radioactive peaks on a Freon column. Four leucyl-tRNA peaks are observed with the homologous hypocotyl system. Hypocotyl synthetase preparations only slightly acylate the other two leucyl-tRNA's (specie...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1969-01, Vol.62 (1), p.202-209 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Anderson, Marianne Bieniek Cherry, Joe H. |
| description | Charged leucyl-transfer RNA (leucyl-tRNA) of soybean cotyledons can be fractionated into six radioactive peaks on a Freon column. Four leucyl-tRNA peaks are observed with the homologous hypocotyl system. Hypocotyl synthetase preparations only slightly acylate the other two leucyl-tRNA's (species 5 and 6). Further, leucyl-tRNA5 and 6 of the hypocotyl are present in small amounts in comparison to the cotyledon. Therefore, the rate-limiting quantities of synthetase and tRNA5 and 6 obtained from the hypocotyl prevent the detection of these two species of tRNA when a homologous hypocotyl system is used. To measure the relative amounts of hypocotyl tRNA's, the cotyledon synthetase preparation is employed. Preliminary results show that 6-benzyladenine increases the acylation of leucyl-tRNA5 and 6 and decreases acylation of tRNA1 in the hypocotyl. Synthetase preparations from cytokinin-treated plants are not significantly affected. |
| doi_str_mv | 10.1073/pnas.62.1.202 |
| format | Article |
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Four leucyl-tRNA peaks are observed with the homologous hypocotyl system. Hypocotyl synthetase preparations only slightly acylate the other two leucyl-tRNA's (species 5 and 6). Further, leucyl-tRNA5 and 6 of the hypocotyl are present in small amounts in comparison to the cotyledon. Therefore, the rate-limiting quantities of synthetase and tRNA5 and 6 obtained from the hypocotyl prevent the detection of these two species of tRNA when a homologous hypocotyl system is used. To measure the relative amounts of hypocotyl tRNA's, the cotyledon synthetase preparation is employed. Preliminary results show that 6-benzyladenine increases the acylation of leucyl-tRNA5 and 6 and decreases acylation of tRNA1 in the hypocotyl. Synthetase preparations from cytokinin-treated plants are not significantly affected.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.62.1.202</identifier><identifier>PMID: 16591727</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Acylation ; Biochemistry ; Biological Sciences: Biochemistry ; Cotyledons ; Enzyme preparations ; Enzymes ; Freon ; Hypocotyls ; RNA ; Soybeans ; Transfer RNA</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1969-01, Vol.62 (1), p.202-209</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-d9fe9d338c26052e731c85e8cc5f8a78432b2ae6cb79f7123c1714052734e71f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/62/1.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/58832$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/58832$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16591727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anderson, Marianne Bieniek</creatorcontrib><creatorcontrib>Cherry, Joe H.</creatorcontrib><title>Differences in Leucyl-Transfer RNA's and Synthetase in Soybean Seedlings</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Charged leucyl-transfer RNA (leucyl-tRNA) of soybean cotyledons can be fractionated into six radioactive peaks on a Freon column. Four leucyl-tRNA peaks are observed with the homologous hypocotyl system. Hypocotyl synthetase preparations only slightly acylate the other two leucyl-tRNA's (species 5 and 6). Further, leucyl-tRNA5 and 6 of the hypocotyl are present in small amounts in comparison to the cotyledon. Therefore, the rate-limiting quantities of synthetase and tRNA5 and 6 obtained from the hypocotyl prevent the detection of these two species of tRNA when a homologous hypocotyl system is used. To measure the relative amounts of hypocotyl tRNA's, the cotyledon synthetase preparation is employed. Preliminary results show that 6-benzyladenine increases the acylation of leucyl-tRNA5 and 6 and decreases acylation of tRNA1 in the hypocotyl. Synthetase preparations from cytokinin-treated plants are not significantly affected.</description><subject>Acylation</subject><subject>Biochemistry</subject><subject>Biological Sciences: Biochemistry</subject><subject>Cotyledons</subject><subject>Enzyme preparations</subject><subject>Enzymes</subject><subject>Freon</subject><subject>Hypocotyls</subject><subject>RNA</subject><subject>Soybeans</subject><subject>Transfer RNA</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><recordid>eNp90DtPwzAUBWALgWh5jCwIoSyoU4ofSWwPDBVvqQIJymy5znUJSp1iJ4j-e1JaSlmYruT7Hds6CB0R3CeYs_OZ06Gf0T7pU0y3UJdgSeIskXgbdTGmPBYJTTpoL4Q3jLFMBd5FHZKlknDKu-juqrAWPDgDISpcNITGzMt45LUL7Xn09DDohUi7PHqeu_oVah1g4Z6r-Rh0OwHysnCTcIB2rC4DHK7mPnq5uR5d3sXDx9v7y8EwNkykdZxLCzJnTBia4ZQCZ8SIFIQxqRWai4TRMdWQmTGXlhPKDOEkaSVnCXBi2T66WN47a8ZTyA242utSzXwx1X6uKl2ovxtXvKpJ9aGoSCVP2nxvlffVewOhVtMiGChL7aBqguKMJZJn3zJeSuOrEDzY9SMEq0X3atG9yqgiqu2-9aebP_vVq7JbcLICi9zPeiN_9s9a2aYsa_isW3e8dG-hrvwapkIwyr4A4fCf8Q</recordid><startdate>19690101</startdate><enddate>19690101</enddate><creator>Anderson, Marianne Bieniek</creator><creator>Cherry, Joe H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19690101</creationdate><title>Differences in Leucyl-Transfer RNA's and Synthetase in Soybean Seedlings</title><author>Anderson, Marianne Bieniek ; Cherry, Joe H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-d9fe9d338c26052e731c85e8cc5f8a78432b2ae6cb79f7123c1714052734e71f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>Acylation</topic><topic>Biochemistry</topic><topic>Biological Sciences: Biochemistry</topic><topic>Cotyledons</topic><topic>Enzyme preparations</topic><topic>Enzymes</topic><topic>Freon</topic><topic>Hypocotyls</topic><topic>RNA</topic><topic>Soybeans</topic><topic>Transfer RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anderson, Marianne Bieniek</creatorcontrib><creatorcontrib>Cherry, Joe H.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Anderson, Marianne Bieniek</au><au>Cherry, Joe H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in Leucyl-Transfer RNA's and Synthetase in Soybean Seedlings</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1969-01-01</date><risdate>1969</risdate><volume>62</volume><issue>1</issue><spage>202</spage><epage>209</epage><pages>202-209</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Charged leucyl-transfer RNA (leucyl-tRNA) of soybean cotyledons can be fractionated into six radioactive peaks on a Freon column. Four leucyl-tRNA peaks are observed with the homologous hypocotyl system. Hypocotyl synthetase preparations only slightly acylate the other two leucyl-tRNA's (species 5 and 6). Further, leucyl-tRNA5 and 6 of the hypocotyl are present in small amounts in comparison to the cotyledon. Therefore, the rate-limiting quantities of synthetase and tRNA5 and 6 obtained from the hypocotyl prevent the detection of these two species of tRNA when a homologous hypocotyl system is used. To measure the relative amounts of hypocotyl tRNA's, the cotyledon synthetase preparation is employed. Preliminary results show that 6-benzyladenine increases the acylation of leucyl-tRNA5 and 6 and decreases acylation of tRNA1 in the hypocotyl. Synthetase preparations from cytokinin-treated plants are not significantly affected.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>16591727</pmid><doi>10.1073/pnas.62.1.202</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1969-01, Vol.62 (1), p.202-209 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmed_primary_16591727 |
| source | Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Acylation Biochemistry Biological Sciences: Biochemistry Cotyledons Enzyme preparations Enzymes Freon Hypocotyls RNA Soybeans Transfer RNA |
| title | Differences in Leucyl-Transfer RNA's and Synthetase in Soybean Seedlings |
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