Monoclonal Antibody Detection of CD46 Clustering beneath Neisseria gonorrhoeae Microcolonies
CD46 (membrane cofactor protein), a complement-regulatory protein that participates in innate and acquired immunity, also serves as a receptor for viral and bacterial pathogens. CD46 isoforms terminate in one of two cytoplasmic tails, Cyt1 or Cyt2, which differ in signaling and trafficking propertie...
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| Veröffentlicht in: | Infection and Immunity 2006-04, Vol.74 (4), p.2428-2435 |
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| creator | Weyand, Nathan J Lee, Shaun W Higashi, Dustin L Cawley, Daniel Yoshihara, Paul So, Magdalene |
| description | CD46 (membrane cofactor protein), a complement-regulatory protein that participates in innate and acquired immunity, also serves as a receptor for viral and bacterial pathogens. CD46 isoforms terminate in one of two cytoplasmic tails, Cyt1 or Cyt2, which differ in signaling and trafficking properties. Dissecting the functions of the two cytoplasmic tails in these cellular processes has been hampered by the absence of specific reagents. Here we report the construction of Cyt1- and Cyt2-specific monoclonal antibodies (MAbs). These MAbs recognize unique epitopes within the tails and can be used for immunofluorescence microscopy, immunoblotting, and immunoprecipitation. Studies of Neisseria gonorrhoeae-infected cells with the CD46 tail MAbs demonstrate the differential recruitment of Cyt1 and Cyt2 to the cortical plaque. |
| doi_str_mv | 10.1128/IAI.74.4.2428-2435.2006 |
| format | Article |
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CD46 isoforms terminate in one of two cytoplasmic tails, Cyt1 or Cyt2, which differ in signaling and trafficking properties. Dissecting the functions of the two cytoplasmic tails in these cellular processes has been hampered by the absence of specific reagents. Here we report the construction of Cyt1- and Cyt2-specific monoclonal antibodies (MAbs). These MAbs recognize unique epitopes within the tails and can be used for immunofluorescence microscopy, immunoblotting, and immunoprecipitation. Studies of Neisseria gonorrhoeae-infected cells with the CD46 tail MAbs demonstrate the differential recruitment of Cyt1 and Cyt2 to the cortical plaque.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.74.4.2428-2435.2006</identifier><identifier>PMID: 16552073</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Amino Acid Sequence ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - metabolism ; Applied microbiology ; Bacterial Adhesion - immunology ; Bacteriology ; Biological and medical sciences ; Cell Line, Tumor ; Cell Polarity - immunology ; Cellular Microbiology: Pathogen-Host Cell Molecular Interactions ; Cytoplasm - immunology ; Epithelial Cells - immunology ; Epithelial Cells - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; Membrane Cofactor Protein - genetics ; Membrane Cofactor Protein - immunology ; Membrane Cofactor Protein - metabolism ; Microbiology ; Microscopy, Fluorescence ; Miscellaneous ; Molecular Sequence Data ; Neisseria gonorrhoeae ; Neisseria gonorrhoeae - immunology ; Neisseria gonorrhoeae - isolation & purification ; Neisseria gonorrhoeae - metabolism ; Protein Isoforms - genetics ; Protein Isoforms - immunology ; Protein Isoforms - metabolism ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><ispartof>Infection and Immunity, 2006-04, Vol.74 (4), p.2428-2435</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright © 2006, American Society for Microbiology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-eb917bbdd7e544590b89bd1582ea78ba86564ce35893d07415ebaf9fb926e0583</citedby><cites>FETCH-LOGICAL-c526t-eb917bbdd7e544590b89bd1582ea78ba86564ce35893d07415ebaf9fb926e0583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1418945/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1418945/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,3177,3178,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17645444$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16552073$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weyand, Nathan J</creatorcontrib><creatorcontrib>Lee, Shaun W</creatorcontrib><creatorcontrib>Higashi, Dustin L</creatorcontrib><creatorcontrib>Cawley, Daniel</creatorcontrib><creatorcontrib>Yoshihara, Paul</creatorcontrib><creatorcontrib>So, Magdalene</creatorcontrib><title>Monoclonal Antibody Detection of CD46 Clustering beneath Neisseria gonorrhoeae Microcolonies</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>CD46 (membrane cofactor protein), a complement-regulatory protein that participates in innate and acquired immunity, also serves as a receptor for viral and bacterial pathogens. CD46 isoforms terminate in one of two cytoplasmic tails, Cyt1 or Cyt2, which differ in signaling and trafficking properties. Dissecting the functions of the two cytoplasmic tails in these cellular processes has been hampered by the absence of specific reagents. Here we report the construction of Cyt1- and Cyt2-specific monoclonal antibodies (MAbs). These MAbs recognize unique epitopes within the tails and can be used for immunofluorescence microscopy, immunoblotting, and immunoprecipitation. Studies of Neisseria gonorrhoeae-infected cells with the CD46 tail MAbs demonstrate the differential recruitment of Cyt1 and Cyt2 to the cortical plaque.</description><subject>Amino Acid Sequence</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - metabolism</subject><subject>Applied microbiology</subject><subject>Bacterial Adhesion - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Tumor</subject><subject>Cell Polarity - immunology</subject><subject>Cellular Microbiology: Pathogen-Host Cell Molecular Interactions</subject><subject>Cytoplasm - immunology</subject><subject>Epithelial Cells - immunology</subject><subject>Epithelial Cells - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Membrane Cofactor Protein - genetics</subject><subject>Membrane Cofactor Protein - immunology</subject><subject>Membrane Cofactor Protein - metabolism</subject><subject>Microbiology</subject><subject>Microscopy, Fluorescence</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Neisseria gonorrhoeae</subject><subject>Neisseria gonorrhoeae - immunology</subject><subject>Neisseria gonorrhoeae - isolation & purification</subject><subject>Neisseria gonorrhoeae - metabolism</subject><subject>Protein Isoforms - genetics</subject><subject>Protein Isoforms - immunology</subject><subject>Protein Isoforms - metabolism</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAURiMEokPhL9CwgF2C7fi5QRpNeYzUwgK6Q7Js5yYxytjFzoD67_FoRi2sWFm2j7_re09VXWDUYkzk2-162wra0pZQIhtCO9YShPijaoWRkg1jhDyuVghh1SjGxVn1LOcfZUsplU-rM8wLgUS3qr5fxxDdHIOZ63VYvI39XX0JC7jFx1DHod5cUl5v5n1eIPkw1hYCmGWqP4PPuRyZeiwRKU0RDNTX3qXoYgn0kJ9XTwYzZ3hxWs-rmw_vv20-NVdfPm4366vGMcKXBqzCwtq-F8AoZQpZqWyPmSRghLRGcsapg45J1fVIUMzAmkENVhEOiMnuvHp3zL3d2x30DsKSzKxvk9-ZdKej8frfm-AnPcZfGlMsFWUl4M0pIMWfe8iL3vnsYJ5NgLjPmgshMEX4vyBWsshAqoDiCJZx5JxguP8NRvqgUBeFWlBN9UGhPijUB4Xl5cu_m3l4d3JWgNcnwGRn5iGZ4Hx-4ASnZYy0cK-O3OTH6bdPoE3eaV-GcV-2MBdHZjBRmzGVnJuvpDSKMOIUlXJ_AD26uno</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Weyand, Nathan J</creator><creator>Lee, Shaun W</creator><creator>Higashi, Dustin L</creator><creator>Cawley, Daniel</creator><creator>Yoshihara, Paul</creator><creator>So, Magdalene</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20060401</creationdate><title>Monoclonal Antibody Detection of CD46 Clustering beneath Neisseria gonorrhoeae Microcolonies</title><author>Weyand, Nathan J ; Lee, Shaun W ; Higashi, Dustin L ; Cawley, Daniel ; Yoshihara, Paul ; So, Magdalene</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-eb917bbdd7e544590b89bd1582ea78ba86564ce35893d07415ebaf9fb926e0583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - metabolism</topic><topic>Applied microbiology</topic><topic>Bacterial Adhesion - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Line, Tumor</topic><topic>Cell Polarity - immunology</topic><topic>Cellular Microbiology: Pathogen-Host Cell Molecular Interactions</topic><topic>Cytoplasm - immunology</topic><topic>Epithelial Cells - immunology</topic><topic>Epithelial Cells - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Membrane Cofactor Protein - genetics</topic><topic>Membrane Cofactor Protein - immunology</topic><topic>Membrane Cofactor Protein - metabolism</topic><topic>Microbiology</topic><topic>Microscopy, Fluorescence</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Neisseria gonorrhoeae</topic><topic>Neisseria gonorrhoeae - immunology</topic><topic>Neisseria gonorrhoeae - isolation & purification</topic><topic>Neisseria gonorrhoeae - metabolism</topic><topic>Protein Isoforms - genetics</topic><topic>Protein Isoforms - immunology</topic><topic>Protein Isoforms - metabolism</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weyand, Nathan J</creatorcontrib><creatorcontrib>Lee, Shaun W</creatorcontrib><creatorcontrib>Higashi, Dustin L</creatorcontrib><creatorcontrib>Cawley, Daniel</creatorcontrib><creatorcontrib>Yoshihara, Paul</creatorcontrib><creatorcontrib>So, Magdalene</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weyand, Nathan J</au><au>Lee, Shaun W</au><au>Higashi, Dustin L</au><au>Cawley, Daniel</au><au>Yoshihara, Paul</au><au>So, Magdalene</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal Antibody Detection of CD46 Clustering beneath Neisseria gonorrhoeae Microcolonies</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>74</volume><issue>4</issue><spage>2428</spage><epage>2435</epage><pages>2428-2435</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>CD46 (membrane cofactor protein), a complement-regulatory protein that participates in innate and acquired immunity, also serves as a receptor for viral and bacterial pathogens. 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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Amino Acid Sequence Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - metabolism Applied microbiology Bacterial Adhesion - immunology Bacteriology Biological and medical sciences Cell Line, Tumor Cell Polarity - immunology Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Cytoplasm - immunology Epithelial Cells - immunology Epithelial Cells - metabolism Fundamental and applied biological sciences. Psychology Humans Membrane Cofactor Protein - genetics Membrane Cofactor Protein - immunology Membrane Cofactor Protein - metabolism Microbiology Microscopy, Fluorescence Miscellaneous Molecular Sequence Data Neisseria gonorrhoeae Neisseria gonorrhoeae - immunology Neisseria gonorrhoeae - isolation & purification Neisseria gonorrhoeae - metabolism Protein Isoforms - genetics Protein Isoforms - immunology Protein Isoforms - metabolism Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) |
| title | Monoclonal Antibody Detection of CD46 Clustering beneath Neisseria gonorrhoeae Microcolonies |
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