Coupling of metabolism and bioconversion: microbial esterification of citronellol with acetyl coenzyme A produced via metabolism of glucose in an interface bioreactor
Microbial esterification of citronellol with acetyl coenzyme A (acetyl-CoA) produced via metabolism of glucose was performed with Hansenula saturnus IFO 0809 in an interface bioreactor by using a hydrophilic carrier (an agar plate or an agar-coated filter pad) and a hydrophobic organic solvent (deca...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1996-07, Vol.62 (7), p.2216-2220 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Oda, S.O. (Kansai Paint Co., Ltd., Hiratsuka, Kanagawa.) Inada, Y Kobayashi, A Kato, A Matsudomi, N Ohta, H |
| description | Microbial esterification of citronellol with acetyl coenzyme A (acetyl-CoA) produced via metabolism of glucose was performed with Hansenula saturnus IFO 0809 in an interface bioreactor by using a hydrophilic carrier (an agar plate or an agar-coated filter pad) and a hydrophobic organic solvent (decane). An increase in the glucose concentration on an agar plate led to a decrease in oxidation of citronellol and an increase in the amount of citronellyl acetate. Fed-batch addition of citronellol efficiently alleviated substrate toxicity and resulted in the accumulation of high levels of citronellyl acetate. Furthermore, triple coupling of acetyl-CoA formation, microbial reduction of citronellal to citronellol, and esterification of the resulting citronellol with acetyl-CoA was also an efficient way to prepare citronellyl acetate. By using coupling systems, citronellyl acetate could be efficiently produced without any acetyl donor |
| doi_str_mv | 10.1128/AEM.62.7.2216-2220.1996 |
| format | Article |
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(Kansai Paint Co., Ltd., Hiratsuka, Kanagawa.) ; Inada, Y ; Kobayashi, A ; Kato, A ; Matsudomi, N ; Ohta, H</creator><creatorcontrib>Oda, S.O. (Kansai Paint Co., Ltd., Hiratsuka, Kanagawa.) ; Inada, Y ; Kobayashi, A ; Kato, A ; Matsudomi, N ; Ohta, H</creatorcontrib><description>Microbial esterification of citronellol with acetyl coenzyme A (acetyl-CoA) produced via metabolism of glucose was performed with Hansenula saturnus IFO 0809 in an interface bioreactor by using a hydrophilic carrier (an agar plate or an agar-coated filter pad) and a hydrophobic organic solvent (decane). An increase in the glucose concentration on an agar plate led to a decrease in oxidation of citronellol and an increase in the amount of citronellyl acetate. Fed-batch addition of citronellol efficiently alleviated substrate toxicity and resulted in the accumulation of high levels of citronellyl acetate. Furthermore, triple coupling of acetyl-CoA formation, microbial reduction of citronellal to citronellol, and esterification of the resulting citronellol with acetyl-CoA was also an efficient way to prepare citronellyl acetate. By using coupling systems, citronellyl acetate could be efficiently produced without any acetyl donor</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.62.7.2216-2220.1996</identifier><identifier>PMID: 16535347</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ACETATE (ESTER) ; ACETATOS (ESTERES) ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ALCOHOLES ; ALCOOL ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; BIOREACTEUR ; BIORREACTORES ; BIOSINTESIS ; BIOSYNTHESE ; Biotechnology ; COENZIMAS ; COENZYME ; Enzymes ; Fundamental and applied biological sciences. 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(Kansai Paint Co., Ltd., Hiratsuka, Kanagawa.)</creatorcontrib><creatorcontrib>Inada, Y</creatorcontrib><creatorcontrib>Kobayashi, A</creatorcontrib><creatorcontrib>Kato, A</creatorcontrib><creatorcontrib>Matsudomi, N</creatorcontrib><creatorcontrib>Ohta, H</creatorcontrib><title>Coupling of metabolism and bioconversion: microbial esterification of citronellol with acetyl coenzyme A produced via metabolism of glucose in an interface bioreactor</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Microbial esterification of citronellol with acetyl coenzyme A (acetyl-CoA) produced via metabolism of glucose was performed with Hansenula saturnus IFO 0809 in an interface bioreactor by using a hydrophilic carrier (an agar plate or an agar-coated filter pad) and a hydrophobic organic solvent (decane). An increase in the glucose concentration on an agar plate led to a decrease in oxidation of citronellol and an increase in the amount of citronellyl acetate. Fed-batch addition of citronellol efficiently alleviated substrate toxicity and resulted in the accumulation of high levels of citronellyl acetate. Furthermore, triple coupling of acetyl-CoA formation, microbial reduction of citronellal to citronellol, and esterification of the resulting citronellol with acetyl-CoA was also an efficient way to prepare citronellyl acetate. By using coupling systems, citronellyl acetate could be efficiently produced without any acetyl donor</description><subject>ACETATE (ESTER)</subject><subject>ACETATOS (ESTERES)</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ALCOHOLES</subject><subject>ALCOOL</subject><subject>Bioconversions. 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(Kansai Paint Co., Ltd., Hiratsuka, Kanagawa.)</creator><creator>Inada, Y</creator><creator>Kobayashi, A</creator><creator>Kato, A</creator><creator>Matsudomi, N</creator><creator>Ohta, H</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960701</creationdate><title>Coupling of metabolism and bioconversion: microbial esterification of citronellol with acetyl coenzyme A produced via metabolism of glucose in an interface bioreactor</title><author>Oda, S.O. 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(Kansai Paint Co., Ltd., Hiratsuka, Kanagawa.)</creatorcontrib><creatorcontrib>Inada, Y</creatorcontrib><creatorcontrib>Kobayashi, A</creatorcontrib><creatorcontrib>Kato, A</creatorcontrib><creatorcontrib>Matsudomi, N</creatorcontrib><creatorcontrib>Ohta, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oda, S.O. (Kansai Paint Co., Ltd., Hiratsuka, Kanagawa.)</au><au>Inada, Y</au><au>Kobayashi, A</au><au>Kato, A</au><au>Matsudomi, N</au><au>Ohta, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coupling of metabolism and bioconversion: microbial esterification of citronellol with acetyl coenzyme A produced via metabolism of glucose in an interface bioreactor</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>62</volume><issue>7</issue><spage>2216</spage><epage>2220</epage><pages>2216-2220</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Microbial esterification of citronellol with acetyl coenzyme A (acetyl-CoA) produced via metabolism of glucose was performed with Hansenula saturnus IFO 0809 in an interface bioreactor by using a hydrophilic carrier (an agar plate or an agar-coated filter pad) and a hydrophobic organic solvent (decane). An increase in the glucose concentration on an agar plate led to a decrease in oxidation of citronellol and an increase in the amount of citronellyl acetate. Fed-batch addition of citronellol efficiently alleviated substrate toxicity and resulted in the accumulation of high levels of citronellyl acetate. Furthermore, triple coupling of acetyl-CoA formation, microbial reduction of citronellal to citronellol, and esterification of the resulting citronellol with acetyl-CoA was also an efficient way to prepare citronellyl acetate. By using coupling systems, citronellyl acetate could be efficiently produced without any acetyl donor</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16535347</pmid><doi>10.1128/AEM.62.7.2216-2220.1996</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | Applied and Environmental Microbiology, 1996-07, Vol.62 (7), p.2216-2220 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pubmed_primary_16535347 |
| source | American Society for Microbiology; PubMed Central; Alma/SFX Local Collection |
| subjects | ACETATE (ESTER) ACETATOS (ESTERES) ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ALCOHOLES ALCOOL Bioconversions. Hemisynthesis Biological and medical sciences BIOREACTEUR BIORREACTORES BIOSINTESIS BIOSYNTHESE Biotechnology COENZIMAS COENZYME Enzymes Fundamental and applied biological sciences. Psychology GLUCOSA GLUCOSE HANSENULA Metabolism METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS Methods. Procedures. Technologies Microbiology Microorganisms REACCIONES QUIMICAS REACTION CHIMIQUE TRIACILGLICEROL LIPASA TRIACYLGLYCEROL LIPASE |
| title | Coupling of metabolism and bioconversion: microbial esterification of citronellol with acetyl coenzyme A produced via metabolism of glucose in an interface bioreactor |
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