Purification and characterization of a novel isozyme of chitinase from Bombyx mori

75-kDa chitinase, which showed potential as a biocontrol agent against Japanese pine sawyer, was characterized after purification from the integument of the fifth instar larvae of Bombyx mori by chromatography on diethylaminoethyl (DEAE)-Toyoperal 650 (M), hydroxylapatite, and Fractogel EMD DEAE 650...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-01, Vol.70 (1), p.252-262
Hauptverfasser:	Kabir, K.E,(Yamaguchi Univ. (Japan). Faculty of Agriculture), Hirowatari, D, Watanabe, K, Koga, D
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Amino Acid Sequence Animals Biological and medical sciences Bombyx - enzymology BOMBYX MORI CHEMICOPHYSICAL PROPERTIES Chitin - chemistry Chitin - metabolism CHITINASE Chitinases - chemistry Chitinases - isolation & purification Chitinases - metabolism CHROMATOGRAPHIE CHROMATOGRAPHY Chromatography, High Pressure Liquid CROMATOGRAFIA Enzyme Stability Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration INSECTICIDAS INSECTICIDE INSECTICIDES Isoenzymes - chemistry Isoenzymes - isolation & purification Isoenzymes - metabolism Kinetics Molecular Sequence Data Molecular Weight properties PROPIEDADES FISICOQUIMICAS PROPRIETE PHYSICOCHIMIQUE PURIFICACION PURIFICATION QUITINASA Substrate Specificity Temperature
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