Microbial Pattern Recognition Receptors Mediate M-Cell Uptake of a Gram-Negative Bacterium

The receptors involved in the sampling of particulate microbial antigens by the gut are largely unknown. Here we demonstrate for the first time in an in vitro M-cell model and in situ in isolated murine intestinal segments that the receptors TLR-4, PAF-R, and [alpha]5{szligbeta}1 integrin are all in...

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Veröffentlicht in:Infection and Immunity 2006, Vol.74 (1), p.625-631
Hauptverfasser: Tyrer, Peter, Foxwell, A. Ruth, Cripps, Allan W, Apicella, Michael A, Kyd, Jennelle M
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creator Tyrer, Peter
Foxwell, A. Ruth
Cripps, Allan W
Apicella, Michael A
Kyd, Jennelle M
description The receptors involved in the sampling of particulate microbial antigens by the gut are largely unknown. Here we demonstrate for the first time in an in vitro M-cell model and in situ in isolated murine intestinal segments that the receptors TLR-4, PAF-R, and [alpha]5{szligbeta}1 integrin are all involved in mediating bacterial uptake associated with transcytosis. The pattern of expression of TLR-4 and [alpha]5{szligbeta}1 integrin differed between M cells and enterocytes. There was increased apical expression of TLR-4 in M-cell cultures, and it was present on the apical surface of murine M cells but not enterocytes in situ. In contrast, PAF-R was expressed equally by both cell types in vitro and was abundantly expressed throughout the intestinal epithelium. Inhibition of TLR-4 and PAF-R, but not TLR-2, reduced gram-negative bacterial uptake by both cell types, whereas inhibition of the apically expressed [alpha]5{szligbeta}1 integrin significantly reduced the ability of M cells to translocate bacteria. Hence, the involvement of each receptor was dependent not only on differences in the level of receptor expression but the cellular localization. Using bacteria that had mutations that affected the bacterial lipooligosaccharide structure indicated that the oligosaccharide moiety was important in bacterial uptake. Taken together, the data suggest that pathogen-associated molecular pattern interactions with pattern recognition receptors are key factors in M-cell recognition of intestinal antigens for mucosal immune priming.
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Ruth</creatorcontrib><creatorcontrib>Cripps, Allan W</creatorcontrib><creatorcontrib>Apicella, Michael A</creatorcontrib><creatorcontrib>Kyd, Jennelle M</creatorcontrib><title>Microbial Pattern Recognition Receptors Mediate M-Cell Uptake of a Gram-Negative Bacterium</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>The receptors involved in the sampling of particulate microbial antigens by the gut are largely unknown. Here we demonstrate for the first time in an in vitro M-cell model and in situ in isolated murine intestinal segments that the receptors TLR-4, PAF-R, and [alpha]5{szligbeta}1 integrin are all involved in mediating bacterial uptake associated with transcytosis. The pattern of expression of TLR-4 and [alpha]5{szligbeta}1 integrin differed between M cells and enterocytes. There was increased apical expression of TLR-4 in M-cell cultures, and it was present on the apical surface of murine M cells but not enterocytes in situ. 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source American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central
subjects Animals
Bacteria
Biological and medical sciences
Caco-2 Cells
Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Enterocytes - immunology
Enterocytes - microbiology
Fundamental and applied biological sciences. Psychology
Haemophilus Infections - immunology
Haemophilus influenzae - immunology
Humans
Intestinal Mucosa - cytology
Intestinal Mucosa - metabolism
Intestinal Mucosa - microbiology
Lipopolysaccharides - chemistry
Male
Mice
Mice, Inbred BALB C
Microbiology
Peyer's Patches - cytology
Peyer's Patches - immunology
Peyer's Patches - metabolism
Peyer's Patches - microbiology
Platelet Membrane Glycoproteins - physiology
Receptors, G-Protein-Coupled - physiology
Receptors, Pattern Recognition - physiology
Toll-Like Receptor 4 - physiology
title Microbial Pattern Recognition Receptors Mediate M-Cell Uptake of a Gram-Negative Bacterium
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