Analysis of fungal pellets by UV-visible spectrum diffuse reflectance spectroscopy
The application of the UV-visible spectrum diffuse reflectance spectroscopy for the determination of intracellular pH in vivo, for determination of cytochrome content, and for the noninvasive in vivo detection of the redox state of fungal mitochondrial cytochromes in filamentous fungi is introduced....
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| Veröffentlicht in: | Applied and Environmental Microbiology 1993-12, Vol.59 (12), p.4253-4260 |
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| container_title | Applied and Environmental Microbiology |
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| creator | Lestan, D Podgornik, H Perdih, A |
| description | The application of the UV-visible spectrum diffuse reflectance spectroscopy for the determination of intracellular pH in vivo, for determination of cytochrome content, and for the noninvasive in vivo detection of the redox state of fungal mitochondrial cytochromes in filamentous fungi is introduced. The time course of the intracellular pH values, mitochondrial cytochromes, and CO-binding pigments content and the correlations between the actual redox state of cytochrome aa3 and saturation of growth medium with oxygen in pellets of the basidiomycete Phanerochaete chrysosporium were determined. As the test microorganism, the yeast Saccharomyces cerevisiae was used. UV-visible spectrum diffuse reflectance spectroscopy proved to be a promising method for the quick and simple analysis of light-impermeable biological structures for which the classical transmittance spectrophotometric methods are difficult to implement |
| doi_str_mv | 10.1128/aem.59.12.4253-4260.1993 |
| format | Article |
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The time course of the intracellular pH values, mitochondrial cytochromes, and CO-binding pigments content and the correlations between the actual redox state of cytochrome aa3 and saturation of growth medium with oxygen in pellets of the basidiomycete Phanerochaete chrysosporium were determined. As the test microorganism, the yeast Saccharomyces cerevisiae was used. UV-visible spectrum diffuse reflectance spectroscopy proved to be a promising method for the quick and simple analysis of light-impermeable biological structures for which the classical transmittance spectrophotometric methods are difficult to implement</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.59.12.4253-4260.1993</identifier><identifier>PMID: 16349122</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteriology ; Biological and medical sciences ; Biotechnology ; CITOCROMOS ; CYTOCHROME ; ESPECTROMETRIA ; Fundamental and applied biological sciences. Psychology ; Fungi ; MEDICION ; MESURE ; Methods ; MICELIO ; Microbiology ; MITOCHONDRIE ; MITOCONDRIA ; MONOXIDO DE CARBONO ; Morphology, structure, chemical composition ; MYCELIUM ; Mycology ; OXYDE DE CARBONE ; PHANEROCHAETE CHRYSOSPORIUM ; PIGMENT ; PIGMENTOS ; POTENCIAL REDOX ; POTENTIEL REDOX ; RADIACION ULTRAVIOLETA ; RAYONNEMENT ULTRAVIOLET ; Scientific imaging ; SPECTROMETRIE</subject><ispartof>Applied and Environmental Microbiology, 1993-12, Vol.59 (12), p.4253-4260</ispartof><rights>1994 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Dec 1993</rights><rights>Copyright © 1993, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c604t-65c0bb1ab6eaa5c3b0075b6c6a5f3e964b73bdf80f71e9a4439fd18d0b73eef83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC195893/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC195893/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3907283$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16349122$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lestan, D</creatorcontrib><creatorcontrib>Podgornik, H</creatorcontrib><creatorcontrib>Perdih, A</creatorcontrib><title>Analysis of fungal pellets by UV-visible spectrum diffuse reflectance spectroscopy</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The application of the UV-visible spectrum diffuse reflectance spectroscopy for the determination of intracellular pH in vivo, for determination of cytochrome content, and for the noninvasive in vivo detection of the redox state of fungal mitochondrial cytochromes in filamentous fungi is introduced. The time course of the intracellular pH values, mitochondrial cytochromes, and CO-binding pigments content and the correlations between the actual redox state of cytochrome aa3 and saturation of growth medium with oxygen in pellets of the basidiomycete Phanerochaete chrysosporium were determined. As the test microorganism, the yeast Saccharomyces cerevisiae was used. UV-visible spectrum diffuse reflectance spectroscopy proved to be a promising method for the quick and simple analysis of light-impermeable biological structures for which the classical transmittance spectrophotometric methods are difficult to implement</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CITOCROMOS</subject><subject>CYTOCHROME</subject><subject>ESPECTROMETRIA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>MEDICION</subject><subject>MESURE</subject><subject>Methods</subject><subject>MICELIO</subject><subject>Microbiology</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>MONOXIDO DE CARBONO</subject><subject>Morphology, structure, chemical composition</subject><subject>MYCELIUM</subject><subject>Mycology</subject><subject>OXYDE DE CARBONE</subject><subject>PHANEROCHAETE CHRYSOSPORIUM</subject><subject>PIGMENT</subject><subject>PIGMENTOS</subject><subject>POTENCIAL REDOX</subject><subject>POTENTIEL REDOX</subject><subject>RADIACION ULTRAVIOLETA</subject><subject>RAYONNEMENT ULTRAVIOLET</subject><subject>Scientific imaging</subject><subject>SPECTROMETRIE</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNp9kUuLFDEUhYMoTjv6B1xIIaKravN-LFwMgy8YENR2G5JU0p2hqlImVSP9703T7YzOwlXg3u_c3HsOAA2Ca4SwfGv8sGZqjfCaYkZainltKEUegBWCSraMEP4QrCBUqsWYwjPwpJRrCCGFXD4GZ4gTqhDGK_D1YjT9vsTSpNCEZdyavpl83_u5NHbfbH60N7FE2_umTN7NeRmaLoawFN9kH_paMqP700zFpWn_FDwKpi_-2ek9B5sP779ffmqvvnz8fHlx1ToO6dxy5qC1yFjujWGOWAgFs9xxwwLxilMriO2ChEEgrwylRIUOyQ7WuvdBknPw7jh3WuzgO-fHOZteTzkOJu91MlH_2xnjTm_TjUaKSUWq_s1Jn9PPxZdZD7G4ersZfVqKFoRQKjnGlXz9XxJxwZgQooIv74HXacnV4aIxZApjrniF5BFy1bBSXbxdGUF9iFfXeDVTGmF9iFcf4tWHeKv0xd8n3wlPeVbg1QkwxZk-5JpOLLccUVBgSe723MXt7lfMXpsy3Pu2Qs-PUDBJm22uczbfVLVECk5-A9BcxA4</recordid><startdate>19931201</startdate><enddate>19931201</enddate><creator>Lestan, D</creator><creator>Podgornik, H</creator><creator>Perdih, A</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19931201</creationdate><title>Analysis of fungal pellets by UV-visible spectrum diffuse reflectance spectroscopy</title><author>Lestan, D ; Podgornik, H ; Perdih, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c604t-65c0bb1ab6eaa5c3b0075b6c6a5f3e964b73bdf80f71e9a4439fd18d0b73eef83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CITOCROMOS</topic><topic>CYTOCHROME</topic><topic>ESPECTROMETRIA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>MEDICION</topic><topic>MESURE</topic><topic>Methods</topic><topic>MICELIO</topic><topic>Microbiology</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>MONOXIDO DE CARBONO</topic><topic>Morphology, structure, chemical composition</topic><topic>MYCELIUM</topic><topic>Mycology</topic><topic>OXYDE DE CARBONE</topic><topic>PHANEROCHAETE CHRYSOSPORIUM</topic><topic>PIGMENT</topic><topic>PIGMENTOS</topic><topic>POTENCIAL REDOX</topic><topic>POTENTIEL REDOX</topic><topic>RADIACION ULTRAVIOLETA</topic><topic>RAYONNEMENT ULTRAVIOLET</topic><topic>Scientific imaging</topic><topic>SPECTROMETRIE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lestan, D</creatorcontrib><creatorcontrib>Podgornik, H</creatorcontrib><creatorcontrib>Perdih, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lestan, D</au><au>Podgornik, H</au><au>Perdih, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of fungal pellets by UV-visible spectrum diffuse reflectance spectroscopy</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>59</volume><issue>12</issue><spage>4253</spage><epage>4260</epage><pages>4253-4260</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>The application of the UV-visible spectrum diffuse reflectance spectroscopy for the determination of intracellular pH in vivo, for determination of cytochrome content, and for the noninvasive in vivo detection of the redox state of fungal mitochondrial cytochromes in filamentous fungi is introduced. The time course of the intracellular pH values, mitochondrial cytochromes, and CO-binding pigments content and the correlations between the actual redox state of cytochrome aa3 and saturation of growth medium with oxygen in pellets of the basidiomycete Phanerochaete chrysosporium were determined. As the test microorganism, the yeast Saccharomyces cerevisiae was used. UV-visible spectrum diffuse reflectance spectroscopy proved to be a promising method for the quick and simple analysis of light-impermeable biological structures for which the classical transmittance spectrophotometric methods are difficult to implement</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16349122</pmid><doi>10.1128/aem.59.12.4253-4260.1993</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1993-12, Vol.59 (12), p.4253-4260 |
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| source | American Society for Microbiology; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacteriology Biological and medical sciences Biotechnology CITOCROMOS CYTOCHROME ESPECTROMETRIA Fundamental and applied biological sciences. Psychology Fungi MEDICION MESURE Methods MICELIO Microbiology MITOCHONDRIE MITOCONDRIA MONOXIDO DE CARBONO Morphology, structure, chemical composition MYCELIUM Mycology OXYDE DE CARBONE PHANEROCHAETE CHRYSOSPORIUM PIGMENT PIGMENTOS POTENCIAL REDOX POTENTIEL REDOX RADIACION ULTRAVIOLETA RAYONNEMENT ULTRAVIOLET Scientific imaging SPECTROMETRIE |
| title | Analysis of fungal pellets by UV-visible spectrum diffuse reflectance spectroscopy |
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