Insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells
Baculoviruses are insect pathogens with a relatively slow speed of action, and this has limited their use as control agents of insect pests. Introduction into baculoviruses of genes which code for proteins interfering specifically with insect metabolism or metamorphosis, such as toxins, hormones, an...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1990-09, Vol.56 (9), p.2764-2770 |
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| creator | Martens, J.W.M. (Agricultural University Wageningen, Wageningen, The Netherlands) Honee, G Zuidema, D Lent, J.W.M. van Visser, B Vlak, J.M |
| description | Baculoviruses are insect pathogens with a relatively slow speed of action, and this has limited their use as control agents of insect pests. Introduction into baculoviruses of genes which code for proteins interfering specifically with insect metabolism or metamorphosis, such as toxins, hormones, and enzymes, may enhance the pathogenicity of these viruses. The complete insecticidal crystal protein gene cryIA(b) of Bacillus thuringiensis subsp. aizawai 7.21 was engineered into the nuclear polyhedrosis virus of Autographa californica (AcNPV) in place of the polyhedrin gene. In infected Spodoptera frugiperda cells, the cryIA(b) gene was expressed at a high level without interference with AcNPV production. The crystal protein was found in the cytoplasm of S. frugiperda cells, mainly as large crystals with an ultrastructure similar to that of B. thuringiensis crystals. Infected-cell extracts inhibited feeding of the large cabbage white Pieris brassicae. The toxicity of the crystal protein expressed by AcNPV recombinants was comparable with that of the crystal protein expressed by a corresponding Escherichia coli recombinant |
| doi_str_mv | 10.1128/aem.56.9.2764-2770.1990 |
| format | Article |
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(Agricultural University Wageningen, Wageningen, The Netherlands) ; Honee, G ; Zuidema, D ; Lent, J.W.M. van ; Visser, B ; Vlak, J.M</creator><creatorcontrib>Martens, J.W.M. (Agricultural University Wageningen, Wageningen, The Netherlands) ; Honee, G ; Zuidema, D ; Lent, J.W.M. van ; Visser, B ; Vlak, J.M</creatorcontrib><description>Baculoviruses are insect pathogens with a relatively slow speed of action, and this has limited their use as control agents of insect pests. Introduction into baculoviruses of genes which code for proteins interfering specifically with insect metabolism or metamorphosis, such as toxins, hormones, and enzymes, may enhance the pathogenicity of these viruses. The complete insecticidal crystal protein gene cryIA(b) of Bacillus thuringiensis subsp. aizawai 7.21 was engineered into the nuclear polyhedrosis virus of Autographa californica (AcNPV) in place of the polyhedrin gene. In infected Spodoptera frugiperda cells, the cryIA(b) gene was expressed at a high level without interference with AcNPV production. The crystal protein was found in the cytoplasm of S. frugiperda cells, mainly as large crystals with an ultrastructure similar to that of B. thuringiensis crystals. Infected-cell extracts inhibited feeding of the large cabbage white Pieris brassicae. The toxicity of the crystal protein expressed by AcNPV recombinants was comparable with that of the crystal protein expressed by a corresponding Escherichia coli recombinant</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.56.9.2764-2770.1990</identifier><identifier>PMID: 16348284</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>AUTOGRAPHA CALIFORNICA ; BACILLUS THURINGIENSIS ; Bacillus thuringiensis aizawai ; BACULOVIRIDAE ; Biological and medical sciences ; Biotechnology ; cloning ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genetic engineering ; Genetic technics ; Genetics and Molecular Biology ; Laboratorium voor Virologie ; Laboratory of Virology ; Methods. Procedures. Technologies ; Modification of gene expression level ; Noctuidae ; nuclear polyhedrosis virus ; Pieridae ; Pieris brassicae ; PODER PATOGENO ; POUVOIR PATHOGENE ; PROTEINAS ; PROTEINE ; RECOMBINACION ; RECOMBINAISON ; Spodoptera frugiperda ; spore crystals ; TOXICIDAD ; TOXICITE</subject><ispartof>Applied and Environmental Microbiology, 1990-09, Vol.56 (9), p.2764-2770</ispartof><rights>1991 INIST-CNRS</rights><rights>Copyright © 1990, American Society for Microbiology</rights><rights>Wageningen University & Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c627t-ba39b19c12c5749f728a1ab1dc1f217a01c7090b71caa9749fe607924e94b6ed3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC184840/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC184840/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19652566$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16348284$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martens, J.W.M. (Agricultural University Wageningen, Wageningen, The Netherlands)</creatorcontrib><creatorcontrib>Honee, G</creatorcontrib><creatorcontrib>Zuidema, D</creatorcontrib><creatorcontrib>Lent, J.W.M. van</creatorcontrib><creatorcontrib>Visser, B</creatorcontrib><creatorcontrib>Vlak, J.M</creatorcontrib><title>Insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Baculoviruses are insect pathogens with a relatively slow speed of action, and this has limited their use as control agents of insect pests. Introduction into baculoviruses of genes which code for proteins interfering specifically with insect metabolism or metamorphosis, such as toxins, hormones, and enzymes, may enhance the pathogenicity of these viruses. The complete insecticidal crystal protein gene cryIA(b) of Bacillus thuringiensis subsp. aizawai 7.21 was engineered into the nuclear polyhedrosis virus of Autographa californica (AcNPV) in place of the polyhedrin gene. In infected Spodoptera frugiperda cells, the cryIA(b) gene was expressed at a high level without interference with AcNPV production. The crystal protein was found in the cytoplasm of S. frugiperda cells, mainly as large crystals with an ultrastructure similar to that of B. thuringiensis crystals. Infected-cell extracts inhibited feeding of the large cabbage white Pieris brassicae. The toxicity of the crystal protein expressed by AcNPV recombinants was comparable with that of the crystal protein expressed by a corresponding Escherichia coli recombinant</description><subject>AUTOGRAPHA CALIFORNICA</subject><subject>BACILLUS THURINGIENSIS</subject><subject>Bacillus thuringiensis aizawai</subject><subject>BACULOVIRIDAE</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cloning</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetics and Molecular Biology</subject><subject>Laboratorium voor Virologie</subject><subject>Laboratory of Virology</subject><subject>Methods. Procedures. Technologies</subject><subject>Modification of gene expression level</subject><subject>Noctuidae</subject><subject>nuclear polyhedrosis virus</subject><subject>Pieridae</subject><subject>Pieris brassicae</subject><subject>PODER PATOGENO</subject><subject>POUVOIR PATHOGENE</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>RECOMBINACION</subject><subject>RECOMBINAISON</subject><subject>Spodoptera frugiperda</subject><subject>spore crystals</subject><subject>TOXICIDAD</subject><subject>TOXICITE</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpVkU2P0zAQhi0EYsvCH-AA4QC3FNtx7PiwB7TiY6WVOMCerbHrtIbELnbS0n-PTaqFvWRi53lmMnoRek3wmhDavQc7rlu-lmsqOKupEPleSvwIrQiWXd02DX-MVhhLWVPK8AV6ltIPjDHDvHuKLghvWEc7tkI_b3yyZnLGbWCoIL8d3HSqQl9BpfPRRpfvTTylKdd9DJN1vrK_99GmZDeVPmUwWhNG7Tz4qUjzEA4uzqnKpPvbvjJ2GNJz9KSHIdkX53qJ7j59_H79pb79-vnm-sNtbTgVU62hkZpIQ6hpBZO9oB0Q0GRjSE-JAEyMwBJrQQyALITlWEjKrGSa201zieTS9whb653PD-UhGpdUAKcGpyPEkzrOUfmhlP2skyKUCpzdq8XNd6PdGOunCIPaRzcWp_gPv3i3U9twUKRjHSv-u7Mfw6_ZpkmNLpXtwdsw5ymtIB3mbQbFApoYUoq2v59BsCoZq5yxarmSqmSsSsaqZJzNV___4j_vHGoG3p4BSAaGPoIvu99zkre05TxzbxZu57a7o4tWQRofjs3My4XpISjYxtzn7pskOK9Amj-czMje</recordid><startdate>19900901</startdate><enddate>19900901</enddate><creator>Martens, J.W.M. (Agricultural University Wageningen, Wageningen, The Netherlands)</creator><creator>Honee, G</creator><creator>Zuidema, D</creator><creator>Lent, J.W.M. van</creator><creator>Visser, B</creator><creator>Vlak, J.M</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SS</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope><scope>QVL</scope></search><sort><creationdate>19900901</creationdate><title>Insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells</title><author>Martens, J.W.M. (Agricultural University Wageningen, Wageningen, The Netherlands) ; Honee, G ; Zuidema, D ; Lent, J.W.M. van ; Visser, B ; Vlak, J.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c627t-ba39b19c12c5749f728a1ab1dc1f217a01c7090b71caa9749fe607924e94b6ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>AUTOGRAPHA CALIFORNICA</topic><topic>BACILLUS THURINGIENSIS</topic><topic>Bacillus thuringiensis aizawai</topic><topic>BACULOVIRIDAE</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>cloning</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetics and Molecular Biology</topic><topic>Laboratorium voor Virologie</topic><topic>Laboratory of Virology</topic><topic>Methods. Procedures. Technologies</topic><topic>Modification of gene expression level</topic><topic>Noctuidae</topic><topic>nuclear polyhedrosis virus</topic><topic>Pieridae</topic><topic>Pieris brassicae</topic><topic>PODER PATOGENO</topic><topic>POUVOIR PATHOGENE</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>Spodoptera frugiperda</topic><topic>spore crystals</topic><topic>TOXICIDAD</topic><topic>TOXICITE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martens, J.W.M. (Agricultural University Wageningen, Wageningen, The Netherlands)</creatorcontrib><creatorcontrib>Honee, G</creatorcontrib><creatorcontrib>Zuidema, D</creatorcontrib><creatorcontrib>Lent, J.W.M. van</creatorcontrib><creatorcontrib>Visser, B</creatorcontrib><creatorcontrib>Vlak, J.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>NARCIS:Publications</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martens, J.W.M. (Agricultural University Wageningen, Wageningen, The Netherlands)</au><au>Honee, G</au><au>Zuidema, D</au><au>Lent, J.W.M. van</au><au>Visser, B</au><au>Vlak, J.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1990-09-01</date><risdate>1990</risdate><volume>56</volume><issue>9</issue><spage>2764</spage><epage>2770</epage><pages>2764-2770</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Baculoviruses are insect pathogens with a relatively slow speed of action, and this has limited their use as control agents of insect pests. Introduction into baculoviruses of genes which code for proteins interfering specifically with insect metabolism or metamorphosis, such as toxins, hormones, and enzymes, may enhance the pathogenicity of these viruses. The complete insecticidal crystal protein gene cryIA(b) of Bacillus thuringiensis subsp. aizawai 7.21 was engineered into the nuclear polyhedrosis virus of Autographa californica (AcNPV) in place of the polyhedrin gene. In infected Spodoptera frugiperda cells, the cryIA(b) gene was expressed at a high level without interference with AcNPV production. The crystal protein was found in the cytoplasm of S. frugiperda cells, mainly as large crystals with an ultrastructure similar to that of B. thuringiensis crystals. Infected-cell extracts inhibited feeding of the large cabbage white Pieris brassicae. The toxicity of the crystal protein expressed by AcNPV recombinants was comparable with that of the crystal protein expressed by a corresponding Escherichia coli recombinant</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>16348284</pmid><doi>10.1128/aem.56.9.2764-2770.1990</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1990-09, Vol.56 (9), p.2764-2770 |
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| subjects | AUTOGRAPHA CALIFORNICA BACILLUS THURINGIENSIS Bacillus thuringiensis aizawai BACULOVIRIDAE Biological and medical sciences Biotechnology cloning EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE GENES Genetic engineering Genetic technics Genetics and Molecular Biology Laboratorium voor Virologie Laboratory of Virology Methods. Procedures. Technologies Modification of gene expression level Noctuidae nuclear polyhedrosis virus Pieridae Pieris brassicae PODER PATOGENO POUVOIR PATHOGENE PROTEINAS PROTEINE RECOMBINACION RECOMBINAISON Spodoptera frugiperda spore crystals TOXICIDAD TOXICITE |
| title | Insecticidal activity of a bacterial crystal protein expressed by a recombinant baculovirus in insect cells |
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