Intranasal immunisation with a 62 kDa proteinase combined with cholera toxin or CpG adjuvant protects against Trichomonas vaginalis genital tract infections in mice
Trichomonosis, caused by the protozoan parasite Trichomonas vaginalis, is one of the most frequent sexually transmitted diseases and is widely spread in all continents. Trichomonas vaginalis as well as other protozoan organisms have high levels of proteolitic activity mainly of the cysteine–proteina...
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| Veröffentlicht in: | International journal for parasitology 2005-11, Vol.35 (13), p.1333-1337 |
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| container_title | International journal for parasitology |
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| creator | Hernández, Hilda M. Figueredo, Mabel Garrido, Nidia Sánchez, Lizet Sarracent, Jorge |
| description | Trichomonosis, caused by the protozoan parasite
Trichomonas vaginalis, is one of the most frequent sexually transmitted diseases and is widely spread in all continents.
Trichomonas vaginalis as well as other protozoan organisms have high levels of proteolitic activity mainly of the cysteine–proteinase type. This activity is necessary for recognition and adhesion of the parasite to the superficial epithelial cells of the host. In the present study, we show that intranasal immunisation with a 62
kDa cysteine–proteinase purified from
T. vaginalis excretion–secretion products in combination with cholera toxin or with synthetic oligodeoxynucleotides (ODN) that contain unmethylated CpG motifs (CpG–ODN) elicits 62
kDa specific IgG and IgA in vaginal lavage fluid and specific IgG in serum. This immunisation protocol resulted in enhanced elimination of parasites following intravaginal challenge of BALB/c mice. |
| doi_str_mv | 10.1016/j.ijpara.2005.08.010 |
| format | Article |
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Trichomonas vaginalis, is one of the most frequent sexually transmitted diseases and is widely spread in all continents.
Trichomonas vaginalis as well as other protozoan organisms have high levels of proteolitic activity mainly of the cysteine–proteinase type. This activity is necessary for recognition and adhesion of the parasite to the superficial epithelial cells of the host. In the present study, we show that intranasal immunisation with a 62
kDa cysteine–proteinase purified from
T. vaginalis excretion–secretion products in combination with cholera toxin or with synthetic oligodeoxynucleotides (ODN) that contain unmethylated CpG motifs (CpG–ODN) elicits 62
kDa specific IgG and IgA in vaginal lavage fluid and specific IgG in serum. This immunisation protocol resulted in enhanced elimination of parasites following intravaginal challenge of BALB/c mice.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/j.ijpara.2005.08.010</identifier><identifier>PMID: 16202417</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvant ; Adjuvants, Immunologic ; Administration, Intranasal ; Animals ; Antibodies, Protozoan - biosynthesis ; Antigens, Protozoan - immunology ; Biological and medical sciences ; Cholera Toxin - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Immunization - methods ; Immunoglobulin A - biosynthesis ; Immunoglobulin G - biosynthesis ; Life cycle. Host-agent relationship. Pathogenesis ; Mice ; Mice, Inbred BALB C ; Oligodeoxyribonucleotides - immunology ; Peptide Hydrolases - immunology ; Protection ; Proteinase ; Protozoa ; Protozoan Vaccines - immunology ; Trichomonas vaginalis ; Trichomonas vaginalis - immunology ; Trichomonas Vaginitis - immunology ; Trichomonas Vaginitis - prevention & control ; Vagina - immunology</subject><ispartof>International journal for parasitology, 2005-11, Vol.35 (13), p.1333-1337</ispartof><rights>2005 Australian Society for Parasitology Inc</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpara.2005.08.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17230622$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16202417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hernández, Hilda M.</creatorcontrib><creatorcontrib>Figueredo, Mabel</creatorcontrib><creatorcontrib>Garrido, Nidia</creatorcontrib><creatorcontrib>Sánchez, Lizet</creatorcontrib><creatorcontrib>Sarracent, Jorge</creatorcontrib><title>Intranasal immunisation with a 62 kDa proteinase combined with cholera toxin or CpG adjuvant protects against Trichomonas vaginalis genital tract infections in mice</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Trichomonosis, caused by the protozoan parasite
Trichomonas vaginalis, is one of the most frequent sexually transmitted diseases and is widely spread in all continents.
Trichomonas vaginalis as well as other protozoan organisms have high levels of proteolitic activity mainly of the cysteine–proteinase type. This activity is necessary for recognition and adhesion of the parasite to the superficial epithelial cells of the host. In the present study, we show that intranasal immunisation with a 62
kDa cysteine–proteinase purified from
T. vaginalis excretion–secretion products in combination with cholera toxin or with synthetic oligodeoxynucleotides (ODN) that contain unmethylated CpG motifs (CpG–ODN) elicits 62
kDa specific IgG and IgA in vaginal lavage fluid and specific IgG in serum. This immunisation protocol resulted in enhanced elimination of parasites following intravaginal challenge of BALB/c mice.</description><subject>Adjuvant</subject><subject>Adjuvants, Immunologic</subject><subject>Administration, Intranasal</subject><subject>Animals</subject><subject>Antibodies, Protozoan - biosynthesis</subject><subject>Antigens, Protozoan - immunology</subject><subject>Biological and medical sciences</subject><subject>Cholera Toxin - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunization - methods</subject><subject>Immunoglobulin A - biosynthesis</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Oligodeoxyribonucleotides - immunology</subject><subject>Peptide Hydrolases - immunology</subject><subject>Protection</subject><subject>Proteinase</subject><subject>Protozoa</subject><subject>Protozoan Vaccines - immunology</subject><subject>Trichomonas vaginalis</subject><subject>Trichomonas vaginalis - immunology</subject><subject>Trichomonas Vaginitis - immunology</subject><subject>Trichomonas Vaginitis - prevention & control</subject><subject>Vagina - immunology</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1u1TAQhS0EoreFN0DIG5YJ45_8bZDQLZRKldiUtTVxfG8nJE5k-97C-_CguEoRq5nFd47OzGHsnYBSgKg_jiWNKwYsJUBVQluCgBdsJ9qmK0Co6iXbAUgomkp0F-wyxhFAVErr1-xC1BKkFs2O_bn1KaDHiBOneT55ipho8fyR0gNHXkv-8xr5GpbkKGOO22XuybthI-zDMrmAPC2_yPMl8P16w3EYT2f0aZPZFDkekXxM_D5QVsxLduJnPGbHiSI_Ok8pB8hJbOLkD1mTM8S88pmse8NeHXCK7u3zvGI_vn65338r7r7f3O4_3xVOdjoV4lC1yupOCKw7XTVNpy1oMQyda3HQ0HfKgVVVU_et1crhUENVt6JRtlWqr9UVe7_5rqd-doNZA80Yfpt_78rAh2cAo8XpkD9nKf7nGqmgljJznzbO5bRncsFES85bN1DIt5lhISPAPPVoRrP1aJ56NNCa3KP6C8UPk3E</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Hernández, Hilda M.</creator><creator>Figueredo, Mabel</creator><creator>Garrido, Nidia</creator><creator>Sánchez, Lizet</creator><creator>Sarracent, Jorge</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20051101</creationdate><title>Intranasal immunisation with a 62 kDa proteinase combined with cholera toxin or CpG adjuvant protects against Trichomonas vaginalis genital tract infections in mice</title><author>Hernández, Hilda M. ; Figueredo, Mabel ; Garrido, Nidia ; Sánchez, Lizet ; Sarracent, Jorge</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e294t-1f583c4911a69457794c041dd9e8ad40b93e0c3576b8c43ead60568173c833b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adjuvant</topic><topic>Adjuvants, Immunologic</topic><topic>Administration, Intranasal</topic><topic>Animals</topic><topic>Antibodies, Protozoan - biosynthesis</topic><topic>Antigens, Protozoan - immunology</topic><topic>Biological and medical sciences</topic><topic>Cholera Toxin - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunization - methods</topic><topic>Immunoglobulin A - biosynthesis</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Oligodeoxyribonucleotides - immunology</topic><topic>Peptide Hydrolases - immunology</topic><topic>Protection</topic><topic>Proteinase</topic><topic>Protozoa</topic><topic>Protozoan Vaccines - immunology</topic><topic>Trichomonas vaginalis</topic><topic>Trichomonas vaginalis - immunology</topic><topic>Trichomonas Vaginitis - immunology</topic><topic>Trichomonas Vaginitis - prevention & control</topic><topic>Vagina - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hernández, Hilda M.</creatorcontrib><creatorcontrib>Figueredo, Mabel</creatorcontrib><creatorcontrib>Garrido, Nidia</creatorcontrib><creatorcontrib>Sánchez, Lizet</creatorcontrib><creatorcontrib>Sarracent, Jorge</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hernández, Hilda M.</au><au>Figueredo, Mabel</au><au>Garrido, Nidia</au><au>Sánchez, Lizet</au><au>Sarracent, Jorge</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intranasal immunisation with a 62 kDa proteinase combined with cholera toxin or CpG adjuvant protects against Trichomonas vaginalis genital tract infections in mice</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>35</volume><issue>13</issue><spage>1333</spage><epage>1337</epage><pages>1333-1337</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>Trichomonosis, caused by the protozoan parasite
Trichomonas vaginalis, is one of the most frequent sexually transmitted diseases and is widely spread in all continents.
Trichomonas vaginalis as well as other protozoan organisms have high levels of proteolitic activity mainly of the cysteine–proteinase type. This activity is necessary for recognition and adhesion of the parasite to the superficial epithelial cells of the host. In the present study, we show that intranasal immunisation with a 62
kDa cysteine–proteinase purified from
T. vaginalis excretion–secretion products in combination with cholera toxin or with synthetic oligodeoxynucleotides (ODN) that contain unmethylated CpG motifs (CpG–ODN) elicits 62
kDa specific IgG and IgA in vaginal lavage fluid and specific IgG in serum. This immunisation protocol resulted in enhanced elimination of parasites following intravaginal challenge of BALB/c mice.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16202417</pmid><doi>10.1016/j.ijpara.2005.08.010</doi><tpages>5</tpages></addata></record> |
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| subjects | Adjuvant Adjuvants, Immunologic Administration, Intranasal Animals Antibodies, Protozoan - biosynthesis Antigens, Protozoan - immunology Biological and medical sciences Cholera Toxin - immunology Female Fundamental and applied biological sciences. Psychology Immunization - methods Immunoglobulin A - biosynthesis Immunoglobulin G - biosynthesis Life cycle. Host-agent relationship. Pathogenesis Mice Mice, Inbred BALB C Oligodeoxyribonucleotides - immunology Peptide Hydrolases - immunology Protection Proteinase Protozoa Protozoan Vaccines - immunology Trichomonas vaginalis Trichomonas vaginalis - immunology Trichomonas Vaginitis - immunology Trichomonas Vaginitis - prevention & control Vagina - immunology |
| title | Intranasal immunisation with a 62 kDa proteinase combined with cholera toxin or CpG adjuvant protects against Trichomonas vaginalis genital tract infections in mice |
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