The neuroprotective KDI domain of gamma 1-laminin is a universal and potent inhibitor of ionotropic glutamate receptors
Previous work from this laboratory indicates that the KDI (Lys-Asp-Ile) domain of gamma 1-laminin promotes functional regeneration of adult rat spinal cord injuries and protects adult rat hippocampal neurons against massive neuronal death induced by intracerebral injection of the glutamate analogue...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroscience research 2005-09, Vol.81 (6), p.797 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 6 |
| container_start_page | 797 |
| container_title | Journal of neuroscience research |
| container_volume | 81 |
| creator | Möykkynen, Tommi Liebkind, Ron Sjöberg, Jari Korpi, Esa R Liesi, Päivi |
| description | Previous work from this laboratory indicates that the KDI (Lys-Asp-Ile) domain of gamma 1-laminin promotes functional regeneration of adult rat spinal cord injuries and protects adult rat hippocampal neurons against massive neuronal death induced by intracerebral injection of the glutamate analogue kainic acid. In the present study, we used patch clamp recordings on cultured human embryonic neocortical neurons and HEK 293 cells expressing recombinant glutamate receptor subunits to study a putative interaction of the KDI with the glutamate system. We show that the KDI domain of gamma 1-laminin is a universal and potent inhibitor of AMPA, kainate, and NMDA subclasses of glutamate receptors, with a noncompetitive action on the AMPA receptor channel activity. Glutamate neurotoxicity plays a key role in both CNS trauma and neurodegenerative disorders, so this unexpected, novel function of the gamma 1-laminin-derived tripeptide may prove clinically valuable in treatment of CNS trauma and/or disease. |
| doi_str_mv | 10.1002/jnr.20523 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmed_primary_16044429</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16044429</sourcerecordid><originalsourceid>FETCH-LOGICAL-p139t-951c70b28d95f82a6674b2acd8a5122a67f0cdd97f6966deea2866819db502323</originalsourceid><addsrcrecordid>eNo1kE1OwzAUhL0A0dKy4ALIF0h5thMnXqLyV1GJTbuuXmKndRXbkeOAuD1BwGo0o29mMYTcMlgxAH5_9nHFoeDigsxBSMhyYHxGrofhDABKFeKKzJiEPM-5mpPP3clQb8YY-hiSaZL9MPTtcUN1cGg9DS09onNIWdahs36K7ECRjn4C44AdRa9pP1V9otafbG1TiD81G3xI06xt6LEbEzpMhkbTmH4ChiW5bLEbzM2fLsj--Wm3fs227y-b9cM265lQKVMFa0qoeaVV0VYcpSzzmmOjKywYn3zZQqO1KluppNTGIK-krJjSdQFccLEgd7-7_Vg7ow99tA7j1-H_AfENu8RcaA</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The neuroprotective KDI domain of gamma 1-laminin is a universal and potent inhibitor of ionotropic glutamate receptors</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Möykkynen, Tommi ; Liebkind, Ron ; Sjöberg, Jari ; Korpi, Esa R ; Liesi, Päivi</creator><creatorcontrib>Möykkynen, Tommi ; Liebkind, Ron ; Sjöberg, Jari ; Korpi, Esa R ; Liesi, Päivi</creatorcontrib><description>Previous work from this laboratory indicates that the KDI (Lys-Asp-Ile) domain of gamma 1-laminin promotes functional regeneration of adult rat spinal cord injuries and protects adult rat hippocampal neurons against massive neuronal death induced by intracerebral injection of the glutamate analogue kainic acid. In the present study, we used patch clamp recordings on cultured human embryonic neocortical neurons and HEK 293 cells expressing recombinant glutamate receptor subunits to study a putative interaction of the KDI with the glutamate system. We show that the KDI domain of gamma 1-laminin is a universal and potent inhibitor of AMPA, kainate, and NMDA subclasses of glutamate receptors, with a noncompetitive action on the AMPA receptor channel activity. Glutamate neurotoxicity plays a key role in both CNS trauma and neurodegenerative disorders, so this unexpected, novel function of the gamma 1-laminin-derived tripeptide may prove clinically valuable in treatment of CNS trauma and/or disease.</description><identifier>ISSN: 0360-4012</identifier><identifier>DOI: 10.1002/jnr.20523</identifier><identifier>PMID: 16044429</identifier><language>eng</language><publisher>United States</publisher><subject>Cell Line ; Cells, Cultured ; Electrophysiology ; Excitatory Amino Acid Antagonists ; Humans ; Immunohistochemistry ; Laminin - pharmacology ; Membrane Potentials - physiology ; Nerve Regeneration - drug effects ; Neurons - physiology ; Neuroprotective Agents - pharmacology ; Patch-Clamp Techniques ; Receptors, AMPA - drug effects ; Receptors, Glutamate - drug effects ; Receptors, N-Methyl-D-Aspartate - drug effects</subject><ispartof>Journal of neuroscience research, 2005-09, Vol.81 (6), p.797</ispartof><rights>(c) 2005 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16044429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Möykkynen, Tommi</creatorcontrib><creatorcontrib>Liebkind, Ron</creatorcontrib><creatorcontrib>Sjöberg, Jari</creatorcontrib><creatorcontrib>Korpi, Esa R</creatorcontrib><creatorcontrib>Liesi, Päivi</creatorcontrib><title>The neuroprotective KDI domain of gamma 1-laminin is a universal and potent inhibitor of ionotropic glutamate receptors</title><title>Journal of neuroscience research</title><addtitle>J Neurosci Res</addtitle><description>Previous work from this laboratory indicates that the KDI (Lys-Asp-Ile) domain of gamma 1-laminin promotes functional regeneration of adult rat spinal cord injuries and protects adult rat hippocampal neurons against massive neuronal death induced by intracerebral injection of the glutamate analogue kainic acid. In the present study, we used patch clamp recordings on cultured human embryonic neocortical neurons and HEK 293 cells expressing recombinant glutamate receptor subunits to study a putative interaction of the KDI with the glutamate system. We show that the KDI domain of gamma 1-laminin is a universal and potent inhibitor of AMPA, kainate, and NMDA subclasses of glutamate receptors, with a noncompetitive action on the AMPA receptor channel activity. Glutamate neurotoxicity plays a key role in both CNS trauma and neurodegenerative disorders, so this unexpected, novel function of the gamma 1-laminin-derived tripeptide may prove clinically valuable in treatment of CNS trauma and/or disease.</description><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Electrophysiology</subject><subject>Excitatory Amino Acid Antagonists</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Laminin - pharmacology</subject><subject>Membrane Potentials - physiology</subject><subject>Nerve Regeneration - drug effects</subject><subject>Neurons - physiology</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Patch-Clamp Techniques</subject><subject>Receptors, AMPA - drug effects</subject><subject>Receptors, Glutamate - drug effects</subject><subject>Receptors, N-Methyl-D-Aspartate - drug effects</subject><issn>0360-4012</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1OwzAUhL0A0dKy4ALIF0h5thMnXqLyV1GJTbuuXmKndRXbkeOAuD1BwGo0o29mMYTcMlgxAH5_9nHFoeDigsxBSMhyYHxGrofhDABKFeKKzJiEPM-5mpPP3clQb8YY-hiSaZL9MPTtcUN1cGg9DS09onNIWdahs36K7ECRjn4C44AdRa9pP1V9otafbG1TiD81G3xI06xt6LEbEzpMhkbTmH4ChiW5bLEbzM2fLsj--Wm3fs227y-b9cM265lQKVMFa0qoeaVV0VYcpSzzmmOjKywYn3zZQqO1KluppNTGIK-krJjSdQFccLEgd7-7_Vg7ow99tA7j1-H_AfENu8RcaA</recordid><startdate>20050915</startdate><enddate>20050915</enddate><creator>Möykkynen, Tommi</creator><creator>Liebkind, Ron</creator><creator>Sjöberg, Jari</creator><creator>Korpi, Esa R</creator><creator>Liesi, Päivi</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>20050915</creationdate><title>The neuroprotective KDI domain of gamma 1-laminin is a universal and potent inhibitor of ionotropic glutamate receptors</title><author>Möykkynen, Tommi ; Liebkind, Ron ; Sjöberg, Jari ; Korpi, Esa R ; Liesi, Päivi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p139t-951c70b28d95f82a6674b2acd8a5122a67f0cdd97f6966deea2866819db502323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Electrophysiology</topic><topic>Excitatory Amino Acid Antagonists</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Laminin - pharmacology</topic><topic>Membrane Potentials - physiology</topic><topic>Nerve Regeneration - drug effects</topic><topic>Neurons - physiology</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Patch-Clamp Techniques</topic><topic>Receptors, AMPA - drug effects</topic><topic>Receptors, Glutamate - drug effects</topic><topic>Receptors, N-Methyl-D-Aspartate - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Möykkynen, Tommi</creatorcontrib><creatorcontrib>Liebkind, Ron</creatorcontrib><creatorcontrib>Sjöberg, Jari</creatorcontrib><creatorcontrib>Korpi, Esa R</creatorcontrib><creatorcontrib>Liesi, Päivi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Möykkynen, Tommi</au><au>Liebkind, Ron</au><au>Sjöberg, Jari</au><au>Korpi, Esa R</au><au>Liesi, Päivi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The neuroprotective KDI domain of gamma 1-laminin is a universal and potent inhibitor of ionotropic glutamate receptors</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J Neurosci Res</addtitle><date>2005-09-15</date><risdate>2005</risdate><volume>81</volume><issue>6</issue><spage>797</spage><pages>797-</pages><issn>0360-4012</issn><abstract>Previous work from this laboratory indicates that the KDI (Lys-Asp-Ile) domain of gamma 1-laminin promotes functional regeneration of adult rat spinal cord injuries and protects adult rat hippocampal neurons against massive neuronal death induced by intracerebral injection of the glutamate analogue kainic acid. In the present study, we used patch clamp recordings on cultured human embryonic neocortical neurons and HEK 293 cells expressing recombinant glutamate receptor subunits to study a putative interaction of the KDI with the glutamate system. We show that the KDI domain of gamma 1-laminin is a universal and potent inhibitor of AMPA, kainate, and NMDA subclasses of glutamate receptors, with a noncompetitive action on the AMPA receptor channel activity. Glutamate neurotoxicity plays a key role in both CNS trauma and neurodegenerative disorders, so this unexpected, novel function of the gamma 1-laminin-derived tripeptide may prove clinically valuable in treatment of CNS trauma and/or disease.</abstract><cop>United States</cop><pmid>16044429</pmid><doi>10.1002/jnr.20523</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0360-4012 |
| ispartof | Journal of neuroscience research, 2005-09, Vol.81 (6), p.797 |
| issn | 0360-4012 |
| language | eng |
| recordid | cdi_pubmed_primary_16044429 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Cell Line Cells, Cultured Electrophysiology Excitatory Amino Acid Antagonists Humans Immunohistochemistry Laminin - pharmacology Membrane Potentials - physiology Nerve Regeneration - drug effects Neurons - physiology Neuroprotective Agents - pharmacology Patch-Clamp Techniques Receptors, AMPA - drug effects Receptors, Glutamate - drug effects Receptors, N-Methyl-D-Aspartate - drug effects |
| title | The neuroprotective KDI domain of gamma 1-laminin is a universal and potent inhibitor of ionotropic glutamate receptors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T15%3A33%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20neuroprotective%20KDI%20domain%20of%20gamma%201-laminin%20is%20a%20universal%20and%20potent%20inhibitor%20of%20ionotropic%20glutamate%20receptors&rft.jtitle=Journal%20of%20neuroscience%20research&rft.au=M%C3%B6ykkynen,%20Tommi&rft.date=2005-09-15&rft.volume=81&rft.issue=6&rft.spage=797&rft.pages=797-&rft.issn=0360-4012&rft_id=info:doi/10.1002/jnr.20523&rft_dat=%3Cpubmed%3E16044429%3C/pubmed%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/16044429&rfr_iscdi=true |