Photobiological and Ultrastructural Studies of Nanoparticles of Poly(lactic-co-glycolic acid)-Containing Bacteriochlorophyll-a as a Photosensitizer Useful for PDT Treatment
The interaction of polymeric nanoparticles formulated from the biodegradable polymer poly(DL-lactide-co-glycolide) loaded with bacteriochlorophyll-a was studied in homogeneous solution and in vitro in the presence of a macrophage cell line (P388-D1-ATCC). Photodynamic therapy (PDT) activity after di...
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| Veröffentlicht in: | Drug delivery 2005-05, Vol.12 (3), p.159-164 |
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| creator | Gomes, Anderson J. Lunardi, Laurelúcia O. Marchetti, Juliana M. Lunardi, Claure N. Tedesco, Antonio C. |
| description | The interaction of polymeric nanoparticles formulated from the biodegradable polymer poly(DL-lactide-co-glycolide) loaded with bacteriochlorophyll-a was studied in homogeneous solution and in vitro in the presence of a macrophage cell line (P388-D1-ATCC). Photodynamic therapy (PDT) activity after different laser doses also was investigated. Scanning electron microscopy analysis of cell phagocyte nanoparticles showed that after 30 min of incubation most of the nanoparticles are in a clear adhesion process to the cell surface. The majority of nanoparticles became phagocytic after 2 hr of incubation time. After laser irradiation of the dye-containing system a total photodamage by nanoparticle phagocyte cells was observed and the cell survival was quantified by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test. Our results indicate that polymeric nanoparticles work as an efficient drug delivery system for PDT drugs. This approach can be widely used for many other hydrophobic photosensitizers with higher aggregation tendency in neoplastic cell treatment. |
| doi_str_mv | 10.1080/10717540590931846 |
| format | Article |
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Photodynamic therapy (PDT) activity after different laser doses also was investigated. Scanning electron microscopy analysis of cell phagocyte nanoparticles showed that after 30 min of incubation most of the nanoparticles are in a clear adhesion process to the cell surface. The majority of nanoparticles became phagocytic after 2 hr of incubation time. After laser irradiation of the dye-containing system a total photodamage by nanoparticle phagocyte cells was observed and the cell survival was quantified by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test. Our results indicate that polymeric nanoparticles work as an efficient drug delivery system for PDT drugs. 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This approach can be widely used for many other hydrophobic photosensitizers with higher aggregation tendency in neoplastic cell treatment.</description><subject>Animals</subject><subject>Bacteriochlorophyll A - chemistry</subject><subject>Bacteriochlorophyll A - pharmacology</subject><subject>Bacteriochlorophyll A - therapeutic use</subject><subject>Bacteriochlorophyll-a</subject><subject>Cell Line</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - radiation effects</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Data Interpretation, Statistical</subject><subject>Lactic Acid - chemistry</subject><subject>Light</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - radiation effects</subject><subject>Macrophages - ultrastructure</subject><subject>Microscopy, Electron, Scanning - methods</subject><subject>Nanoparticle</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - ultrastructure</subject><subject>Particle Size</subject><subject>Phagocytosis - drug effects</subject><subject>Phagocytosis - radiation effects</subject><subject>Photochemotherapy - methods</subject><subject>Photodynamic Therapy</subject><subject>Photosensitizing Agents - chemistry</subject><subject>Photosensitizing Agents - pharmacology</subject><subject>Photosensitizing Agents - therapeutic use</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Polylactic Acid-Polyglycolic Acid Copolymer</subject><subject>Polymers - chemistry</subject><subject>Technology, Pharmaceutical - methods</subject><subject>Time Factors</subject><subject>Ultrastructural</subject><issn>1071-7544</issn><issn>1521-0464</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2KFDEUhQtRnHH0AdxIVuIsSvNfFXSj7S8M2mD3uriVSnVlSCc9SYqhfCYf0mg3iAizSrj3O4fLOVX1lOCXBLf4FcENaQTHQmHFSMvlveqcCEpqzCW_X_5lXxeAn1WPUrrGGLeEiofVGZGYipaL8-rnego59Da4sLMaHAI_oK3LEVKOs85zLLPveR6sSSiM6Cv4cICYrXbHwTq45YUDXSa1DvXOLTo4qxFoO1zWq-AzWG_9Dr0rjIk26MmFGA7T4lwNCBIC9OeGZHyy2f4wEW2TGWeHxhDR-v0GbaKBvDc-P64ejOCSeXJ6L6rtxw-b1ef66tunL6u3V7XmmOe6Z0PLgRgmgTPMCVWqkRRLJkbagxG0gb5VilElZUNg5EqSBrOBmr5nvaLsonp-9D3EcDOblLu9Tdo4B96EOXWyxVwJ0RSQHEEdQ0rRjN0h2j3EpSO4-11R919FRfPsZD73ezP8VZw6KcCbI2B9SWAPtyG6ocuwlNzGCF7b1LG7_F__I58MuDxpiKa7DnP0Jbg7rvsFXDG0QA</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Gomes, Anderson J.</creator><creator>Lunardi, Laurelúcia O.</creator><creator>Marchetti, Juliana M.</creator><creator>Lunardi, Claure N.</creator><creator>Tedesco, Antonio C.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Photobiological and Ultrastructural Studies of Nanoparticles of Poly(lactic-co-glycolic acid)-Containing Bacteriochlorophyll-a as a Photosensitizer Useful for PDT Treatment</title><author>Gomes, Anderson J. ; Lunardi, Laurelúcia O. ; Marchetti, Juliana M. ; Lunardi, Claure N. ; Tedesco, Antonio C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-b3d84a1e36a430412997620635f2bae527ab8993296671af4961703d2ebb3b923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Bacteriochlorophyll A - chemistry</topic><topic>Bacteriochlorophyll A - pharmacology</topic><topic>Bacteriochlorophyll A - therapeutic use</topic><topic>Bacteriochlorophyll-a</topic><topic>Cell Line</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - radiation effects</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>Data Interpretation, Statistical</topic><topic>Lactic Acid - chemistry</topic><topic>Light</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - radiation effects</topic><topic>Macrophages - ultrastructure</topic><topic>Microscopy, Electron, Scanning - methods</topic><topic>Nanoparticle</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - ultrastructure</topic><topic>Particle Size</topic><topic>Phagocytosis - drug effects</topic><topic>Phagocytosis - radiation effects</topic><topic>Photochemotherapy - methods</topic><topic>Photodynamic Therapy</topic><topic>Photosensitizing Agents - chemistry</topic><topic>Photosensitizing Agents - pharmacology</topic><topic>Photosensitizing Agents - therapeutic use</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Polylactic Acid-Polyglycolic Acid Copolymer</topic><topic>Polymers - chemistry</topic><topic>Technology, Pharmaceutical - methods</topic><topic>Time Factors</topic><topic>Ultrastructural</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gomes, Anderson J.</creatorcontrib><creatorcontrib>Lunardi, Laurelúcia O.</creatorcontrib><creatorcontrib>Marchetti, Juliana M.</creatorcontrib><creatorcontrib>Lunardi, Claure N.</creatorcontrib><creatorcontrib>Tedesco, Antonio C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Drug delivery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gomes, Anderson J.</au><au>Lunardi, Laurelúcia O.</au><au>Marchetti, Juliana M.</au><au>Lunardi, Claure N.</au><au>Tedesco, Antonio C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photobiological and Ultrastructural Studies of Nanoparticles of Poly(lactic-co-glycolic acid)-Containing Bacteriochlorophyll-a as a Photosensitizer Useful for PDT Treatment</atitle><jtitle>Drug delivery</jtitle><addtitle>Drug Deliv</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>12</volume><issue>3</issue><spage>159</spage><epage>164</epage><pages>159-164</pages><issn>1071-7544</issn><eissn>1521-0464</eissn><abstract>The interaction of polymeric nanoparticles formulated from the biodegradable polymer poly(DL-lactide-co-glycolide) loaded with bacteriochlorophyll-a was studied in homogeneous solution and in vitro in the presence of a macrophage cell line (P388-D1-ATCC). Photodynamic therapy (PDT) activity after different laser doses also was investigated. Scanning electron microscopy analysis of cell phagocyte nanoparticles showed that after 30 min of incubation most of the nanoparticles are in a clear adhesion process to the cell surface. The majority of nanoparticles became phagocytic after 2 hr of incubation time. After laser irradiation of the dye-containing system a total photodamage by nanoparticle phagocyte cells was observed and the cell survival was quantified by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide test. Our results indicate that polymeric nanoparticles work as an efficient drug delivery system for PDT drugs. This approach can be widely used for many other hydrophobic photosensitizers with higher aggregation tendency in neoplastic cell treatment.</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>16025845</pmid><doi>10.1080/10717540590931846</doi><tpages>6</tpages></addata></record> |
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| ispartof | Drug delivery, 2005-05, Vol.12 (3), p.159-164 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Taylor & Francis:Master (3349 titles) |
| subjects | Animals Bacteriochlorophyll A - chemistry Bacteriochlorophyll A - pharmacology Bacteriochlorophyll A - therapeutic use Bacteriochlorophyll-a Cell Line Cell Membrane - drug effects Cell Membrane - radiation effects Cell Membrane - ultrastructure Cell Survival - drug effects Cell Survival - radiation effects Data Interpretation, Statistical Lactic Acid - chemistry Light Macrophages - drug effects Macrophages - radiation effects Macrophages - ultrastructure Microscopy, Electron, Scanning - methods Nanoparticle Nanostructures - chemistry Nanostructures - ultrastructure Particle Size Phagocytosis - drug effects Phagocytosis - radiation effects Photochemotherapy - methods Photodynamic Therapy Photosensitizing Agents - chemistry Photosensitizing Agents - pharmacology Photosensitizing Agents - therapeutic use Polyglycolic Acid - chemistry Polylactic Acid-Polyglycolic Acid Copolymer Polymers - chemistry Technology, Pharmaceutical - methods Time Factors Ultrastructural |
| title | Photobiological and Ultrastructural Studies of Nanoparticles of Poly(lactic-co-glycolic acid)-Containing Bacteriochlorophyll-a as a Photosensitizer Useful for PDT Treatment |
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