reporter gene system used to study developmental expression of alternative oxidase and isolate mitochondrial retrograde regulation mutants in Arabidopsis

Perturbation of mitochondrial function causes altered nuclear gene expression in plants. To study this response, called mitochondrial retrograde regulation, and developmental gene expression, a transgenic Arabidopsis thaliana (Col-0) line containing a firefly luciferase gene controlled by a promoter...

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Veröffentlicht in:	Plant molecular biology 2005-04, Vol.57 (6), p.871-888
Hauptverfasser:	Zarkovic, J, Anderson, S.L, Rhoads, D.M
Format:	Artikel
Sprache:	eng
Schlagworte:	alternative oxidase Antimycin A - pharmacology Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - physiology Arabidopsis thaliana AtAOX1a gene Blotting, Northern Cotyledon - genetics Cotyledon - metabolism cotyledons DNA, Bacterial - genetics DNA, Plant - chemistry DNA, Plant - genetics electron transport chain Fluoroacetates - pharmacology gene expression gene expression regulation Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Enzymologic - drug effects Gene Expression Regulation, Plant - drug effects genes Genes, Reporter - genetics leaves luciferase Luciferases - genetics Luciferases - metabolism Luminescent Measurements - methods messenger RNA mitochondria Mitochondria - drug effects Mitochondria - physiology Mitochondrial Proteins Models, Biological Molecular Sequence Data Mutagenesis, Insertional mutants Mutation nucleocytoplasmic interaction nucleotide sequences oxidoreductases Oxidoreductases - genetics Plant Leaves - genetics Plant Leaves - metabolism Plant Proteins Plants, Genetically Modified promoter regions Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism reporter genes RNA, Plant - genetics RNA, Plant - metabolism Seeds - drug effects Seeds - genetics Sequence Analysis, DNA Signal Transduction - drug effects transgenic plants
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