Immunofluorescence Assay for Detection of the Nucleocapsid Antigen of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Cells Derived from Throat Wash Samples of Patients with SARS
An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-as...
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| Veröffentlicht in: | Journal of Clinical Microbiology 2005-05, Vol.43 (5), p.2444-2448 |
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| container_title | Journal of Clinical Microbiology |
| container_volume | 43 |
| creator | Liu, I-Jung Chen, Pei-Jer Yeh, Shiou-Hwei Chiang, Yu-Ping Huang, Li-Min Chang, Ming-Fu Chen, Shey-Ying Yang, Pan-Chyr Chang, Shan-Chwen Wang, Wei-Kung |
| description | An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future. |
| doi_str_mv | 10.1128/JCM.43.5.2444-2448.2005 |
| format | Article |
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It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.43.5.2444-2448.2005</identifier><identifier>PMID: 15872279</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Antigens, Viral - analysis ; Biological and medical sciences ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Nucleocapsid - analysis ; Pharynx - pathology ; Pharynx - virology ; SARS coronavirus ; SARS Virus - immunology ; SARS Virus - isolation & purification ; Severe Acute Respiratory Syndrome - diagnosis ; Severe Acute Respiratory Syndrome - pathology ; Virology</subject><ispartof>Journal of Clinical Microbiology, 2005-05, Vol.43 (5), p.2444-2448</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright © 2005, American Society for Microbiology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-d12b4730ed888e144ae368659f5adfbb59ab3a78ce2008dc6555fd91418fc4483</citedby><cites>FETCH-LOGICAL-c596t-d12b4730ed888e144ae368659f5adfbb59ab3a78ce2008dc6555fd91418fc4483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153760/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1153760/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16765008$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15872279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, I-Jung</creatorcontrib><creatorcontrib>Chen, Pei-Jer</creatorcontrib><creatorcontrib>Yeh, Shiou-Hwei</creatorcontrib><creatorcontrib>Chiang, Yu-Ping</creatorcontrib><creatorcontrib>Huang, Li-Min</creatorcontrib><creatorcontrib>Chang, Ming-Fu</creatorcontrib><creatorcontrib>Chen, Shey-Ying</creatorcontrib><creatorcontrib>Yang, Pan-Chyr</creatorcontrib><creatorcontrib>Chang, Shan-Chwen</creatorcontrib><creatorcontrib>Wang, Wei-Kung</creatorcontrib><creatorcontrib>SARS Research Group of the National Taiwan University College of Medicine-National Taiwan University Hospital</creatorcontrib><creatorcontrib>the SARS Research Group of the National Taiwan University College of Medicine-National Taiwan University Hospital</creatorcontrib><title>Immunofluorescence Assay for Detection of the Nucleocapsid Antigen of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Cells Derived from Throat Wash Samples of Patients with SARS</title><title>Journal of Clinical Microbiology</title><addtitle>J Clin Microbiol</addtitle><description>An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future.</description><subject>Antigens, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Nucleocapsid - analysis</subject><subject>Pharynx - pathology</subject><subject>Pharynx - virology</subject><subject>SARS coronavirus</subject><subject>SARS Virus - immunology</subject><subject>SARS Virus - isolation & purification</subject><subject>Severe Acute Respiratory Syndrome - diagnosis</subject><subject>Severe Acute Respiratory Syndrome - pathology</subject><subject>Virology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1u1DAUhSMEokPhFahZgGCRwU7i2NlUGg1_ReVHTSvYWR7neuIqiQfbmWoekbfC0Yw6sGJjL-53zrWPTpKcETwnJONvPy-_zIt8TudZURRpPPg8w5g-SGYEVzwtS_zzYTLDuKIpITk7SZ54f4sxKQpKHycnhHKWZayaJb8v-n4crO5G68ArGBSghfdyh7R16B0EUMHYAVmNQgvo66g6sEpuvGnQYghmDfezGrbgolqNAdAV-I1xMli3Q_VuaJztAb2uF1f1mzT6W2VkgAYtrbOD3Bo3emQGtISu83GrM9s41FGErltnZUA_pG9RLftNB35a-F0GA0Pw6M6EOIi-T5NHWnYenh3u0-Tmw_vr5af08tvHi-XiMlW0KkPakGxVsBxDwzmHGIiEvOQlrTSVjV6taCVXuWRcQQyUN6qklOqmIgXhWsWc89PkfO-7GVc9NDGy4GQnNs700u2ElUb8OxlMK9Z2KwihOStxNHh1MHD21wg-iN7E5LtODmBHL0rGKl5U2X9BwoqKFmRyZHtQOeu9A33_GoLF1BcR-yKKXFAx9WU6uJj6EpXP__7MUXcoSAReHgDpley0k4My_siVrKQxp8i92HOtWbd3xoGQvhe3qj-ujczZntHSCrl20eemzjDJY08rlmGW_wHjJ-IN</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Liu, I-Jung</creator><creator>Chen, Pei-Jer</creator><creator>Yeh, Shiou-Hwei</creator><creator>Chiang, Yu-Ping</creator><creator>Huang, Li-Min</creator><creator>Chang, Ming-Fu</creator><creator>Chen, Shey-Ying</creator><creator>Yang, Pan-Chyr</creator><creator>Chang, Shan-Chwen</creator><creator>Wang, Wei-Kung</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20050501</creationdate><title>Immunofluorescence Assay for Detection of the Nucleocapsid Antigen of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Cells Derived from Throat Wash Samples of Patients with SARS</title><author>Liu, I-Jung ; Chen, Pei-Jer ; Yeh, Shiou-Hwei ; Chiang, Yu-Ping ; Huang, Li-Min ; Chang, Ming-Fu ; Chen, Shey-Ying ; Yang, Pan-Chyr ; Chang, Shan-Chwen ; Wang, Wei-Kung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-d12b4730ed888e144ae368659f5adfbb59ab3a78ce2008dc6555fd91418fc4483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antigens, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Nucleocapsid - analysis</topic><topic>Pharynx - pathology</topic><topic>Pharynx - virology</topic><topic>SARS coronavirus</topic><topic>SARS Virus - immunology</topic><topic>SARS Virus - isolation & purification</topic><topic>Severe Acute Respiratory Syndrome - diagnosis</topic><topic>Severe Acute Respiratory Syndrome - pathology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, I-Jung</creatorcontrib><creatorcontrib>Chen, Pei-Jer</creatorcontrib><creatorcontrib>Yeh, Shiou-Hwei</creatorcontrib><creatorcontrib>Chiang, Yu-Ping</creatorcontrib><creatorcontrib>Huang, Li-Min</creatorcontrib><creatorcontrib>Chang, Ming-Fu</creatorcontrib><creatorcontrib>Chen, Shey-Ying</creatorcontrib><creatorcontrib>Yang, Pan-Chyr</creatorcontrib><creatorcontrib>Chang, Shan-Chwen</creatorcontrib><creatorcontrib>Wang, Wei-Kung</creatorcontrib><creatorcontrib>SARS Research Group of the National Taiwan University College of Medicine-National Taiwan University Hospital</creatorcontrib><creatorcontrib>the SARS Research Group of the National Taiwan University College of Medicine-National Taiwan University Hospital</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Clinical Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, I-Jung</au><au>Chen, Pei-Jer</au><au>Yeh, Shiou-Hwei</au><au>Chiang, Yu-Ping</au><au>Huang, Li-Min</au><au>Chang, Ming-Fu</au><au>Chen, Shey-Ying</au><au>Yang, Pan-Chyr</au><au>Chang, Shan-Chwen</au><au>Wang, Wei-Kung</au><aucorp>SARS Research Group of the National Taiwan University College of Medicine-National Taiwan University Hospital</aucorp><aucorp>the SARS Research Group of the National Taiwan University College of Medicine-National Taiwan University Hospital</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunofluorescence Assay for Detection of the Nucleocapsid Antigen of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Cells Derived from Throat Wash Samples of Patients with SARS</atitle><jtitle>Journal of Clinical Microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>43</volume><issue>5</issue><spage>2444</spage><epage>2448</epage><pages>2444-2448</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. 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| ispartof | Journal of Clinical Microbiology, 2005-05, Vol.43 (5), p.2444-2448 |
| issn | 0095-1137 1098-660X |
| language | eng |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antigens, Viral - analysis Biological and medical sciences Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Humans Infectious diseases Medical sciences Microbiology Nucleocapsid - analysis Pharynx - pathology Pharynx - virology SARS coronavirus SARS Virus - immunology SARS Virus - isolation & purification Severe Acute Respiratory Syndrome - diagnosis Severe Acute Respiratory Syndrome - pathology Virology |
| title | Immunofluorescence Assay for Detection of the Nucleocapsid Antigen of the Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus in Cells Derived from Throat Wash Samples of Patients with SARS |
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